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Hydrolytic driving force. The hydrolysis of pyrophosphate to orthophosphate is important in driving forwards biosynthetic reactions such as the synthesis of DNA. THis hydrolytic reaction is catalyzed in E. coli by a pyrophosphatase that has a mass of 120 kd and consists of 6 identical subunits. For this enzyme, a unit of activity is defined as the amount of enzyme that hydrolyzes 10µmol of pyrophosphate in 15min at 37degrees celsius under std assay conditions. The purified enzyme has a Vmax of 2800 units per milligram of enzyme.
(a)How many moles of substrate are hydrolyzed persecond per milligram of enzyme when the substrate conc is much greater than KM?
(b)How many moles of active sites are there in 1mg of enzyme? Assume that each subunit has one active site.
(c)What's the turnover number of the enzyme? Compare this value with others mentioned in this chapter.
What are the cells that produce the myelin sheath? Of which substance is the myelin sheath formed? In the central nervous system (CNS) the myelin sheath is made by apposition o
ROLE OF PSYCHIATRIC NURSE: In Blocks 2 and 3 you have learnt about various therapeutic interventions for mental disorders like anxiety neurotic disorders, psychotic disorder
Megasporogenesis One of the nucellar cells situated subjacent to the epidermis differentiates into the primary archesporial cell. This cell is larger than the adjacent ce
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Fusion inhibitor After HIV binds to the host cell surface, a conformational change occurs in the transmembrane glycoprotein sub- unit (gp41) of the viral envelope, facilitatin
A 62 year old white male develops pitting edema of both feet. Put on Lasix and metoprolol. Develops hallucinations and confusion. Tapered off. Dies 3 weeks later of lymphatic o
Q. Explain States of matter Ans. Chemistry deals with the nature and composition of matter - anything that occupies space and has mass, and the changes which it undergoes. Matt
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