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In yeast, a new mutation called suc was recently discovered. The suc mutant cannot use sucrose as a source of carbon, and will not grow if the culture medium contains only sucrose as a carbon source. A plasmid library made with cDNA prepared from wild type yeast was used to transform a strain of yeast with two mutations: suc and ura. In this strain the URA gene, which allows yeast to grow in medium depleted in uracil, has been deleted. After transformation the yeast cells were plated on culture medium without uracil and containing sucrose as the only carbon source. a) Why must the plasmid chosen to construct the library contain a functional URA gene? b) Although 80% of the yeast were transformed, only a fraction of the transformed yeast formed colonies on the medium. Could this experiment be called a complementation assay? Explain your reasoning. c) The recombinant plasmid extracted from the surviving colonies contains a gene called SUC2 that codes for convertase, an enzyme needed to metabolize sucrose. Mutations in the SNF gene also lead to an inability to ferment sucrose, but SNF mutants can still ferment glucose. Although the SNF mutants have no mutations in the SUC2 gene, they have no convertase activity. We know that the SNF gene is not involved in translation. Is the SNF gene involved in DNA replication or transcription? Briefly justify your answer. d) The SNF mutation is rescued by another mutation in the gene for histone H4. What is the likely function of the SNF gene?
Explain about the Dietary Fibre? You are all aware that fibre is an important component in the structure of plants. However, fibre as a dietaiy constituent was considered impor
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Antimicrobial Prophylaxis - Controversial The need for prophylaxis in breast surgery, herniorraphy and other "clean" surgical procedures has been controversial. Medical Letter
Ablation experiment is an experiment designed to produce an animal deficient in one or the few cell types, in order to study cell lineage or the cell function. The logic is to mak
In his experiments, Mendel noted that when two traits are involved in a genetic cross, they are inherited independently of each other. The reason for this is that A. genes on the s
a) The elongation level of translation in eukaryotes requires three elongation factors, eEF-IB, eEF-1A and eEF-2, that have similar functions to their prokaryotic counterparts EF-G
#applications of biotechnology..
How will the plant-incorporated protectants rules change EPA's current registration process? The rules clarify which plant-incorporated protectants are needed to be evaluated u
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