What is the likely function of the snf gene, Biology

Assignment Help:

In yeast, a new mutation called suc was recently discovered.  The suc mutant cannot use sucrose as a source of carbon, and will not grow if the culture medium contains only sucrose as a carbon source.  A plasmid library made with cDNA prepared from wild type yeast was used to transform a strain of yeast with two mutations: suc and ura.  In this strain the URA gene, which allows yeast to grow in medium depleted in uracil, has been deleted. After transformation the yeast cells were plated on culture medium without uracil and containing sucrose as the only carbon source.
 
a)  Why must the plasmid chosen to construct the library contain a functional URA gene?
 
b)  Although 80% of the yeast were transformed, only a fraction of the transformed yeast
formed colonies on the medium.  Could this experiment be called a complementation
assay?  Explain your reasoning.
 
c)  The recombinant plasmid extracted from the surviving colonies contains a gene called
SUC2 that codes for convertase, an enzyme needed to metabolize sucrose.  Mutations in
the SNF gene also lead to an inability to ferment sucrose, but SNF mutants can still
ferment glucose.  Although the SNF mutants have no mutations in the SUC2 gene, they
have no convertase activity.  We know that the SNF gene is not involved in translation. 
Is the SNF gene involved in DNA replication or transcription?  Briefly justify your
answer.
 
d)  The SNF mutation is rescued by another mutation in the gene for histone H4.  What is
the likely function of the SNF gene?


Related Discussions:- What is the likely function of the snf gene

Discuss about troponin receptor site, Skeletal muscle A. The myosin hea...

Skeletal muscle A. The myosin head is activated (energized) by the conversion of GTP (that is bound to myosin) to GDP and Pi (that are bound to myosin). B. Detachment of the

Compare between isp and soy flour, Compare between ISP and soy flour Th...

Compare between ISP and soy flour The cost of isolated soybean proteins is five to seven times higher than that of defatted soy flour. On an equivalent protein weight basis, th

What do you understand by polyp?, What do you understand by Polyp? The ...

What do you understand by Polyp? The sessile, asexual stage in the cnidarian life cycle. In some species they are independent organisms; in others, they form colonies where som

Explain the carbohydrates and fat requirements during sepsis, Explain the C...

Explain the Carbohydrates and Fat Requirements during Sepsis? Carbohydrates should constitute nearly 60% to 70% of the total energy. Glucose is the primary calorie substrate in

Riccia, What isthe Riccia

What isthe Riccia

Can you explain the anoxia, Q. What is the anoxia? Anoxia is a situatio...

Q. What is the anoxia? Anoxia is a situation in which there is no available oxygen in the cell without oxygen the respiratory chain stops there is no ATP production the cell do

What kind of agent causes sars, Q. SARS is a disease that appeared in 2003 ...

Q. SARS is a disease that appeared in 2003 with epidemic features in the province of Guangdong, in east China. What kind of agent causes SARS? SARS is caused by a virus from th

Find which two amino acids make up this pocket, Sickling occurs in deoxyhem...

Sickling occurs in deoxyhemoglobin, but not in oxyhemoglobin. Normal deoxyhemoglobin (HbA and HbS) molecules have a small hydrophobic "pocket" on the surface of a ? chain. In oxyhe

Define effect of enery on quality and quantity of human milk, Define Effect...

Define Effect of Enery on quality and quantity of human milk? Energy: In case of chronic under nutrition, an association between postpartum weight loss and lower energy transfe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd