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In at least single eukaryote, Tetrahymena, the pre-rRNA molecule holds an intron. Erasing of the intron in running processing of the pre-rRNA does not require the assistance of any protein! Instead, in the presence of guanosine, GDP, GMP or GTP, the intron excises itself is a phenomenon known as self- splicing. This was the first expression of ribozymes, which is, catalytic RNA molecules which catalyze particular reactions. The Self-splicing introns have also been discovered in some eukaryotic mRNAs and even peptidyl transferase a main activity in protein synthesis is now known to be a ribozyme.
You now want to express the gene in E coli, but unfortunately it has an intron in it that will not be processed out in bacteria, hence it must be removed. (a) Design P
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Cidofovir (Vistide) IV cidofovir given once weekly for 2 weeks and then once every 2 weeks for maintenance therapy can delay progression of CMV retinitis in patients with AIDS
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