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Reaction specificity
Some enzymes catalyze only one reaction acting on a specific substrate. Example: urease and catalase acts only on urea and hydrogen peroxide, respectively. This is also called absolute specificity.
Many enzymes can catalyze same type of reactions (phosphate transfer, oxidation-reduction, hydrolysis etc.) in several structurally-related compounds. Example: carboxypeptidase acts on protein chains and removes one amino acid at a time from the C-terminal, irrespective of the nature of zimino acid.
A substrate caa undergo many reactions but in a specific reaction, an enzyme will catitlyze only one of these reactions. Example: citrate synthase converts oxaloacetate to citrate in the presence of acetyl-CoA. But, in absence of acetyl- CoA, oxaloacetate is acted upon by a different enzyme malate dehydrogenase with the formation of malate.
Oxygen concentration When oxygen is abundant, the rate of oxidation is independent of oxygen concentration, but at very low oxygen concentration, the rate is approximately prop
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