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The DNA of a bacterial cell,such as Escherichia coli is a circular double-stranded molecule often referred to as the bacterial chromosome. The circular DNA is packaged into a area of the cell called as the nucleoid where it is organized into 50 or so loops or domains which are bound to a central protein scaffold attached to the cell membrane. Above figure illustrates these industries, though only six loops are shown for clarity. Within this structure the DNA is in fact not a circular double-stranded DNA molecule like as that shown in fig. but is negatively supercoiled, which is, it is twisted upon itself and is also completed with several DNA-binding proteins, the most common of that are proteins HU, H-NS and HLP-1. These are histone-like proteins.
Figure: (a) The association of circular bacterial DNA with a protein scaffold; (b) a circular double-stranded DNA molecule; (c) supercoiled DNA.
Prior to the solving of the structure of DNA, Erwin Chargaff calculated the amounts of the four nucleotides (A, T,G,C) and made which of the following conclusions? A. All four
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