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What is Polymerase Chain Reactionin genetics?
A great advance in sequencing and cloning DNA is the polymerase chain reaction, devised by the American biochemist Kary Mullis in 1984. By this technique, millions of copies of a DNA sequence can be produced in a medium containing appropriate nucleotides and enzymes. As the name suggests, newly replicated DNA strands serve as templates to build additional copies.
Polymerase chain reaction, or PCR technology, is an important tool in gene sequencing, the determination of the order of nucleotides in a DNA strand, because it is possible to characterize as little as a single molecule of DNA. It is useful in medical forensics, or medical detective work, since DNA "fingerprints" are unique to each individual, and tiny quantities of tissue containing DNA may be left at a crime scene. PCR technology is also invaluable in early diagnosing of diseases where organisms may be present in such small quantities that they have not yet caused detectable antibody production.
Coordination of Digestion You have learnt that digestion is a process in which large food molecules arc broken down step by step into their constituents. In primitive metazoa
You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in yo
character and classification of pigeon
A 20 year old female university student came to the clinic complaining of palpitations. She has had occasional rapid heart rates but never lasting more than 2 minutes. This time it
How does the federal government regulate plant-incorporated protectants and ensure their safe use in foods? EPA regulates the manufacture, sale and use of pesticides, containin
Manifestations of anemia that are directly due to the diminished oxygen-carrying capacity of hemoglobin include: Answer A. pale skin. B. bleeding. C. bone pain. D. fatigue.
Define Counter Stain - Staining Technique? Finally, the smear is counter stained with a simple basic dye different in colour from crystal violet. Safranin is the most commonly
Enumerate the Number of converging developments These include: 1) A better definition of the unique developmental concepts that are applicable to infants and young children,
Q. Define Pacinian corpuscules? Pacinian corpuscules are an example of sensory receptors scattered deep in the subcutaneous tissue underlying skin or in viscera. These mech
Q. Derive Gorlin Formula? Formula I: First Hydraulic Formula (Toricelli's law) F = AVCc Where, F = flow rate A = orifice area Cc = coefficient of orifice cont
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