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What is PCR? How does PCR works?
PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.
First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.
Flowering and Endogenous Rhythms The last four decades of studies in animals and plant physiology have clearly established the existence of a biological clock mechanism to me
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The Carbohydrate sets are covalently attached to various variant proteins to form glycoproteins. Carbohydrates are a shorter percentage of the weight of glycoproteins than of prote
Liver necrosis?
Explain Phenylephine and Methoxamine in amyl nitrite ? They have opposing effects to amyl nitrite as they increase the systemic BP. phenylephine due to its shorter duration of
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