What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Why is drosophila a convenient animal study of linked genes, Why is drosoph...

Why is drosophila a convenient animal for the study of linked genes? The fruit fly drosophila is appropriate for the study of Genetics because it presents many distinct traits

Suicide cells, significant role of suicide cells?

significant role of suicide cells?

Replication of chromatin, Whenever a chromosome is replicated the replicati...

Whenever a chromosome is replicated the replication machinery passes by the nucleosomes without deleting the histones from the DNA. How this happens is since not fully understood.

Zoology, general characteristics of phylum chordata

general characteristics of phylum chordata

Thumb rule for dietary management, Q. Thumb rule for dietary management? ...

Q. Thumb rule for dietary management? The thumb rule for dietary management is to advice the patient to try to cut down or avoid:  Red meats. Organ meats such as br

Define field test used to assess aerobic capacity, Define Field Test used t...

Define Field Test used to Assess Aerobic Capacity? A field test that has been used extensively in nutrition studies to assess aerobic capacity measures Heart Rate responses to

How much calories in dried fruits, How much calories in Dried fruits Dr...

How much calories in Dried fruits Dried fruits are exceptionally rich in calories and they supply significant amounts of minerals. Of the vitamins found in fruits, β-Carotene a

Excretion in protozoans, Excretion in Protozoans Excretion of metaboli...

Excretion in Protozoans Excretion of metabolic wastes is dope almost exclusively by diffusion. All protozoans are ammonotelic i.e., the end product of their nitrogen metabolis

Define effect of protein excess during pregnancy period, Define effect of P...

Define effect of Protein excess during pregnancy period? It is not advisable to recommend protein supplements. Reports in the literature indicate that high-protein supplements

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd