What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Translation in prokaryotes, Term Translation or protein synthesis, is quite...

Term Translation or protein synthesis, is quite similar in eukaryotes and prokaryotes. We will look at the details of the process in prokaryotes and consider the differences in euk

Are viruses cellular beings, Are viruses cellular beings? Viruses are m...

Are viruses cellular beings? Viruses are measured as living beings but they do not have cellular structure. There is some argument regarding their classification as living b

Explain elastase, Elastase The inactive  proelastase  is activated by t...

Elastase The inactive  proelastase  is activated by trypsin to the active form elastase. Elastase attacks  peptide  bonds  next  to  the  small amino  acid residues such  3s  g

Microorganisms, what are four types of microorganisms and their definitions...

what are four types of microorganisms and their definitions?

Regulation of egg, difference between mosaic and regulation egg

difference between mosaic and regulation egg

How do derived characters help cladistic taxonomists, How do derived charac...

How do derived characters help cladistic taxonomists determine phylogenetic relationships? A derived character is found only between members of a particular group. Thus, cladi

What type of selection is being demonstrated, If a cow develops a preferenc...

If a cow develops a preference for eating white four o'clock flowers and ignoring pink and red four o'clock flowers, what type of selection is being demonstrated? Would the cow eve

Define feeding and nutritional management of dysphilgia, Define Feeding and...

Define Feeding and Nutritional Management of Dysphilgia? A multidisciplinary approach is essential for successful management of dysphilgia. Patient, nurse, physician, die titi

Physical stress - temperature, Physical Stress - Temperature We are mo...

Physical Stress - Temperature We are most familiar with the plants and other organisms that live at temperatures close to the temperature range in which we are adapted to live

Working of eye, WORKIN G OF EYE - Human eye is sensitive to light wave...

WORKIN G OF EYE - Human eye is sensitive to light wavelenght ranging from 380 - 760 nm. Bees, ants, spiders and gold fish can see uttraviolet light which is invisible to hu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd