What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

What are coacervates, What are coacervates? Coarcervates are small stru...

What are coacervates? Coarcervates are small structures made of the aggregation of organic molecules under water solution. By electrical attraction the molecules join into bigg

Why organism that can not exist in the presence of oxygen, organism that ca...

organism that can not exist in the presence of oxygen ?

Explain working of hypophysis- ovaries endocrine axis, Q. Does the hypophys...

Q. Does the hypophysis- ovaries endocrine axis work in the same way during pregnancy as in non-pregnant women? If pregnancy does not occur how does another menstrual cycle begin?

Class of crustacea - ostracoda, Class of Crustacea - Ostracoda General...

Class of Crustacea - Ostracoda Generally called mussel or seed-shrimps, Ostracoda include both fresh water and marine forms. The small crustaceans, computing a few mm have the

Keys for identification of taxonomic work, Q. Keys for identification of ta...

Q. Keys for identification of taxonomic work? Identification is an integral part of all taxonomic work. It is the recognition of certain, characters of flower, fruit, leaf or s

Cardiac catheterization and angiography, Q.Define Cardiac catheterization a...

Q.Define Cardiac catheterization and angiography? The purpose of this unit is to give you an overview and insight into the world of Cardiac Catheterization and Angiography. Thi

Safety aspects of feed processing, Safety aspects of feed processing S...

Safety aspects of feed processing Safety of the man and machine in a feed mill is very important to avoid the accidents. Some basic aspects of a good safety programme are as f

Role of carbohydrates in dyslipidemia, Q. Role of Carbohydrates in dyslipid...

Q. Role of Carbohydrates in dyslipidemia? It is important to know about these carbohydrates, as they all differ in their digestive properties. The rate of absorption is variabl

An insect collecting net, An insect collecting net A useful insect net ...

An insect collecting net A useful insect net can be made from a round stick such as a broom or mop handle, some heavy wire and mosquito netting or cheese cloth. Bend a heavy pi

What is the window phase of an infection, Q. What is the window phase of an...

Q. What is the window phase of an infection? How is this concept important for the test of HIV infection in blood banks? The primary immune reply of the body facing any infecti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd