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What is PCR? How does PCR works?
PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.
First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.
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Major Criteria 1) Positive blood culture • Typical microorganism for infective endocarditis from two separate blood cultures Viridans streptococci, Streptococcus bovis, HAC
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If you look up a world atlas you would notice that most of the earth's surface is covered by the waters of the oceans (about 71%). Beneath the water surface is a fascinating world
HE P ARIN It is highly sulphated mucopolysaccharide made of alternate units of glucosamine and glucuronic acid. Heparin is mainly produced by liver and mast cells. I
Some observations seen in this fever are: 1. Massive loss of lean body mass or muscle due to tissue breakdown (250-500 g muscle tissue is lost/day) leading to excessive nitro
Width of the Implant The width of the implant especially at the interface area is critical towards the success of the implant. It has been recommended that at least 1mm of bone
Q. What are the physiological systems known as integrative systems? Why is this designation justified? The integrative systems are the endocrine system and the nervous system.
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