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What is PCR? How does PCR works?
PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.
First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.
To show the respiration of a plant Place the plant in a test tube held in a weighted wooden block. Put this in a bowl having lime water and cover the plant with a jar. Keep the
Disaccharides can be understood as follows 1.Sugars made up of the two monosaccharides held together by the covalent bond; like sucrose and lactose . 2. Type of sugar (sacch
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Suppose that you are raising Downton wombats, carefully because they bite when teased.You notice that some of your wombats have white spots, whereas others have solid coats. What t
ERRORS IN FERTILIZATION - 1. Polyspermy - When more than one sperms are fused in an ovum, this condition is called polyspermy. 2. Polygynae - During oogen
In studies of human body, which of the below terms is used to describe a very rapid heart rate or pulse rate? Is it: a) Tachycardia b) Bradycardia c) Cardiosis d) Myoc
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Q. What are the three kinds of respiration in which the circulatory system transports gases? The circulatory system has an important role in branchial respiration, cutaneous re
Locomotion Continuous formation of new pseudopodia keeps amoeba in constant locomotion .This is called amoeboid movement .It occurs in many other protozoans , in amoebo
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