What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Fat cow syndrome, F a t cow syndrome Fat cow syndrome, also known as ...

F a t cow syndrome Fat cow syndrome, also known as fatty infiltration of liver, is a highly fatal disease in high yielding dairy animals that occurs a few days before or afte

Azotobacter, Azotobacter Azotobacter is known to possess the following...

Azotobacter Azotobacter is known to possess the following two mechanisms to protect its nitrogenase from oxygen inhibition. Respiratory protection and Confor

How is reproduction done in beings of the class reptilia, How is reproducti...

How is reproduction done in beings of the class Reptilia? These beings reproduce sexually by internal fecundation by means of copulation among male and female individuals. They

Causes of reduced serum hdl levels, Q. Causes of reduced Serum HDL levels? ...

Q. Causes of reduced Serum HDL levels? Possible causes of reduced Serum HDL levels: - Cigarette smoking - Hypertriglyceridemia - Obesity - Genetic factors - Lack

Define ferricyanide-cyanide reagent or drabkin''s solution, Define Ferricya...

Define Ferricyanide-cyanide reagent or Drabkin's solution? Dissolve 0.2 g potassium ferricyanide, 0.05 g potassium cyanide and 1.0 g sodium bicarbonate in about 100-1 50 ml dis

What is the structural depiction of a carboxyl group, Q. What is the struct...

Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe

Explain the term light reflex - neuronal pathways, Explain the term Light r...

Explain the term Light reflex - Neuronal pathways The neuronal pathway for light reflex can be afferent and efferent. In the afferent pathway, the pupillary fibres begin in the

Deficiency diseases-vitamin- d deficiency, Vitamin- D deficiency Anima...

Vitamin- D deficiency Animals obtain vitamin D either through the diet or when skin is exposed to solar radiation. The deficiency of vitamin D is associated with reduced absor

Homework, #questiocan you help in a biology question?.

#questiocan you help in a biology question?.

Evolution of dental implants, The first evidence of implants dates back to ...

The first evidence of implants dates back to 600 A.D. in the Mayan population. There are reports that the ancient Egyptians attempted intra-osseous implantation of animal teeth or

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd