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What is PCR? How does PCR works?
PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.
First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.
Lingual nerve and artery It is the branch of mandibular nerve which enters the oral cavity above the posterior edge of the mylohyoid muscle close to the 3 rd molar region proc
Define Amino acid requirements for Human? Data regarding the essential amino acid requirements of infants, children and adults are given in terms of egg protein and cow's milk
cellular endosperm diagrammatic view
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Tabulate macro & micro nutrients on the basis of -elements, source, synthesis site, symptoms
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