What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Eucaryotic cell structure , Eucaryotic cell structure All eucaryotic c...

Eucaryotic cell structure All eucaryotic cells have a cytoskeleton made up of a network of protein filaments (Figure shown below). This network gives the cell its shape, capac

What is kingdom protista in diversity of life, What is Kingdom Protista in ...

What is Kingdom Protista in diversity of life? Members of Kingdom Protista are grouped separately from the other eukaryotic kingdoms because most of the organisms have a unicel

What are primary indications for pharmacologic stress, Q. What are primary ...

Q. What are primary indications for pharmacologic stress? The primary indications for pharmacologic stress are inability to exercise or the desire to identify viable myocardium

Mendel''s experimental plant, MENDEL'S EXPERIMENTAL PLANT Mendel select...

MENDEL'S EXPERIMENTAL PLANT Mendel selected common garden pea ( Pisum sativum ) for his experiments, because:- (i)         The pea is small and annual plant. Duration of lif

Name mimicry by which insect mimics insec for protection, From the followin...

From the following, name the type of mimicry by which a palatable, acceptable insect mimics an unfavorable, noxious insec for protection. a) Batesian mimicry (pron: BATE-see-an

Determine the major demographic processes, Determine the major demographic ...

Determine the major demographic processes? There are five major demographic processes, which are continually at work within the population namely, fertility, mortality, marriag

Define risk factors for drug nutrient interaction, Define Clinical Signific...

Define Clinical Significance and Risk Factors for Drug-Nutrient interactions? We are already aware of the fact that poor nutritional status can impair drug metabolism and the d

Asphyxia, buffalo can be dies due ashyxia

buffalo can be dies due ashyxia

What are living and nonliving reservoirs, What are living and nonliving res...

What are living and nonliving reservoirs? Viruses are both living as well as non-living. They have reservoirs of genes. A one nucleotide is a unit of gene. Viral genes make use

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd