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What is PCR? How does PCR works?
PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.
First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.
Pulp Tissue Revascularization : Definition: Is the procedure to re-establish the vitality in non-vital tooth by allowing repair & regeneration of the tissues.
Why is holophytic nutrition a mode of autotrophic nutrition
Heparinisation : The patient should be fully heparinised before the start of cardio pulmonary bypass. Baseline activated clotting time is measured (ACT). 3 mg (300 units) per k
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What is Secondary Growth explain briefly? Secondary Growth in Stems : Young developing shoots increase in height by adding new cells, which are produced by the apical meriste
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