What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Why do leaves change color, Why do leaves change color? Leave is the fo...

Why do leaves change color? Leave is the food factories of plants. During the spring and summer, leaves are actively making food and they are filled with chlorophyll, which giv

Bacteria is an obligate anaerobe, Q. What is signifying when it is said tha...

Q. What is signifying when it is said that a bacteria is an obligate anaerobe? Obligate anaerobes are those living beings that do not survive in the presence of oxygen. For exa

Biochemical changes - consequences of aging, Biochemical Changes - Conseque...

Biochemical Changes - Consequences of Aging Several biochemical changes are correlated with aging. Detailed biochemical studies in rats have been carried out by Prof. M.S. Kan

What are cilia and flagella, Cilia and flagella are structures found in var...

Cilia and flagella are structures found in various prokaryotes as well in some eukaryotic cells. They play defense, nutrition and movement roles for the cell. In eukaryotic cells o

Determine the term - essential element, Determine the term - essential elem...

Determine the term - essential element It becomes difficult to meet all the conditions so as to establish essentiality. This is particularly so for the elements that are requir

Neuropsychological understanding of behavioural deficits, Neuropsychologica...

Neuropsychological understanding of behavioural deficits Behaviour is an outcome of the interaction of the brain with the environment. A composite of multiple psychological pro

Define the phytate and protein interaction, Define the Phytate and Protein ...

Define the Phytate and Protein Interaction? You may be more familiar with phytate's role in mineral absorption. However phytates also bind with starch and protein. Depending on

What is phellogen, What is phellogen? What is its function? Phellogen, ...

What is phellogen? What is its function? Phellogen, also called as cork cambium, is the meristematic plant tissue responsible for the formation of the periderm (the covering of

Protostomes, differences between acoelomate,coelomate and pseudocoelomate

differences between acoelomate,coelomate and pseudocoelomate

Implementation of nursing care in acute rheumatic fever, Implementation of ...

Implementation of Nursing Care Provide Bed Rest and Comfort   Your major role as a nurse is to provide bed rest to the child and assist the child and parents to understa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd