What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Determine the maximum crop yield, Determine the maximum crop yield In ...

Determine the maximum crop yield In determining maximum crop yield it was thought that a given plant let us say corn, is only inherently capable of producing a given amount of

Define fatty acid synthesis, Fatty acid synthesis is the formation of fatty...

Fatty acid synthesis is the formation of fatty acids from malonyl-CoA and acetyl-CoA precursors by action of enzymes called as fatty acid synthases. It is a very significant part o

Explain the procedure of balloon pulmonary valvuloplasty, Q. Explain the pr...

Q. Explain the procedure of Balloon Pulmonary Valvuloplasty? Right heart study is done to measure the transvalvular gradient and exclude supravalvular and subvalvular component

Side effects of sedatives and hypnotics, Side Effects of Sedatives and Hypn...

Side Effects of Sedatives and Hypnotics: Side effects are same  like anti anxiety drugs such as hypotension, palpitation, syncope (fainting), change in libido, physical or psy

Why are most ammoniotelic beings aquatic animals, Q. Why are most ammoniote...

Q. Why are most ammoniotelic beings aquatic animals? Aquatic animals like crustaceans, amphibian larvae and bony fishes generally are ammoniotelic since ammonia diffuses more e

Physical lay out, Physical Lay Out: Physical lay out of the nursing un...

Physical Lay Out: Physical lay out of the nursing unit should be planned and built in such a manner so that it helps the head nurse to discharge her duties/carry  out her resp

Maturation phase, Maturation Phase The Maturation phase characterizes...

Maturation Phase The Maturation phase characterizes he transformation of diploid primary spermatocytes into haploid spermatids. The primary spermatocyte divides meiotically t

Animal tissue, Animal Tissue A tissue is a group of cells that are si...

Animal Tissue A tissue is a group of cells that are similar in structure, origin and function. Tissue word coined by Bichat (Father of Histology). Histology term

Recovery of by-products: coal, Recovery of tar: the coke oven gas coming o...

Recovery of tar: the coke oven gas coming out from ovens is passed through a tower where liquid ammonia is sprayed. The tar and dust are removed and then recovered. Recover of

What possible combinations of chromosomes will be present, A diploid cell c...

A diploid cell contains three pairs of homologous chromosomes designated C1 and C2, M1 and M2, and S1 and S2; no crossing over occurs. What possible combinations of chromosomes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd