What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Phylum colentrata, In phylum colenterata if tissues are present in the body...

In phylum colenterata if tissues are present in the body of an organism then why organs are not present as group of tissues makes an organuestion..

How is dna replication related to cancer, How is DNA replication related to...

How is DNA replication related to cancer? Cancer can result when errors happen in the replication of DNA in genes that control how a cell divides. A mass of cancerous cells kno

Define three major steps for study of genetic family tree, What are the thr...

What are the three major steps for a good study of a genetic family tree? Step 1: Determine whether the studied phenotypical form has a dominant or the recessive pattern. Step

Open heart surgery , Open Heart Surgery These surgeries are done unde...

Open Heart Surgery These surgeries are done under alternative arrangements to continue oxygenated systemic blood supply, while the heart is operated on (Cardiopulmonary bypas

Calculate the level of magnesium in urine, Calculate the Level of Magnesium...

Calculate the Level of Magnesium in urine? Urinary Mg excretion decreases with Mg deficiency. Therefore, Mg excretion before and after administration of an intravenous Mg load

Explain elements of xylem in gymnosperms, The chief water conducting elemen...

The chief water conducting elements of xylem in gymnosperms are: 1. Vessels 2. Fibres 3. Transfusion tissue 4. Tracheids Tracheids

Define feeding and nutritional management of spinal trauma, Define the Feed...

Define the Feeding and Nutritional Management of spinal trauma? The main objectives of nutritional management are to meet the nutritional needs of the initial acute phase and

What is the phenomenon of the apical dominance in plants, What is the pheno...

What is the phenomenon of the apical dominance in plants? How it can be artificially eliminated? The Apical dominance is the phenomenon by which high (over the positive range l

Digestion, explain digestion in protozoans

explain digestion in protozoans

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd