What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

What are the catalysts, Q. What are the catalysts? Catalysts are substa...

Q. What are the catalysts? Catalysts are substances that decrease the activation energy of a chemical reaction, facilitating it or making it energetically viable. The catalyst

Describe digestion of this breakfast, Your breakfast consists of a cup of b...

Your breakfast consists of a cup of black coffee with sugar as well as a plain bagel covered with cream cheese. Describe the digestion of this breakfast as it passes through each m

General characteristics of protozoans, General Characteristics of Protozoan...

General Characteristics of Protozoans The protozoans, are eukaryotes that form a group of about 80,000 single-celled organisms under the kingdom Protista. The unicellular leve

What is the mechanism described by darwin, What is the mechanism described ...

What is the mechanism described by Darwin that eliminates species less adapted to environmental conditions? The mentioned mechanism is the natural selection.

Define hexose monophosphate pathway, Define hexose monophosphate pathway ...

Define hexose monophosphate pathway The hexose monophosphate pathway (HMP also called the pentose phosphate pathway, or phoshogluconate pathway) consists of Mo irreversible o

How is opening and closing of stomata controlled, How is opening and closin...

How is opening and closing of stomata controlled? Describe a) Why is the length of a food chain in an ecosystem generally limited to 3 - 4 trophic levels? Explain with an examp

What is the acrosome of the sperm cell, What is the acrosome of the sperm c...

What is the acrosome of the sperm cell? How is it formed? The acrosome is a structure that has a great number of digestive enzymes, it is located in the anterior end of the spe

Describe the process of rodent control, Q. Describe the process of Rodent c...

Q. Describe the process of Rodent control? Rats and mice are destructive and cause huge loss of stored food commodities. They transmit pathogenic bacteria. Rats and mice are ge

What is the basic structure of a virus, What is the basic structure of a vi...

What is the basic structure of a virus? Viruses are constituted of genetic material (DNA or RNA) covered by a protein capsule also called as a capsid. Some viruses, like HIV, h

Complications of short bowel syndrome, Q. Complications of short bowel synd...

Q. Complications of short bowel syndrome? Complications of short bowel syndrome are generally related to malnutrition. What are the complications of malnutrition? A person with

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd