What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Elaborates congenital aortic stenosis in details, Elaborates Congenital Aor...

Elaborates Congenital Aortic Stenosis in details? More common in males (4: 1). High incidence of Bicuspid Aortic valve. Murmur present from early infancy and sometimes at birth

Disorder of parathyroid function, Disorder of Parathyroid Function: Th...

Disorder of Parathyroid Function: The disorders of parathyroid function may  either  lead  to increased production or decreased production of parathormone.  Hypoparathyroid

Gibberellins - plant growth substances, Gibberellins - Plant Growth Substan...

Gibberellins - Plant Growth Substances In late 1920's Kurosawa observed that the medium in which the fungus Gibberella had grown could cause the abnormal tallness in rice seed

Secretion of parathormone and calcium blood level, Q. What is the relation ...

Q. What is the relation between secretion of parathormone and the calcium blood level? The parathormone increases the calcium blood level since it stimulates the resorption (re

Mechanism of maturation, Mechanism of Maturation The basic mechanism o...

Mechanism of Maturation The basic mechanism of maturation of ovum is more or less similar in all organisms. The primordial germ cells that enter into the ovary divide mitotica

How did the first fermenting autotrophs appeared, How did the first ferment...

How did the first fermenting autotrophs appeared? What about the first aerobic beings? A heterotrophic hypothesis asserts that the first living beings were the fermenting heter

Why are we not inundated with bacterial infections, We are told that every ...

We are told that every surface we touch is teeming with bacterial cells, and bacteria are found in the pools we swim in, the water we wash with, and on the hands of friends. Why ar

Explain properties related to protein-protein interactions, Explain Propert...

Explain Properties related to protein-protein interactions? Properties related to protein-protein interactions include dough formation, one of the important functional properti

Define osseointegration and its theories, Q. Define osseointegration and it...

Q. Define osseointegration and its theories? Osseointegration implies that "it is a contact established without interposition of non bony tissue between normal remodeled bone a

Aquatic ecosystem - types of ecosystem, Aquatic Ecosystem - Types of Ecosys...

Aquatic Ecosystem - Types of Ecosystem Global waters cover about three quarters of the earth's surface either as fresh water where salt content is less than 0.5 per cent or as

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd