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What is PCR? How does PCR works?
PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.
First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.
Action of Hormones We said earlier that hormones are released into the blood stream or extracellular fluid and therefore, reach most of the cells of the body. However, they
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Patient is a 7-year-old girl who was brought to her physician by her mother with complaints of general fatigue, anorexia, and unexplained bruises and "rash" for the past 2 weeks. C
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Describe the clinical significance and symptoms of edema. How does edema (in its different forms listed in your text) appear on medical imaging examinations? Discuss any specific c
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GASES There are 4 gases in the protoplasm which remain dissolved in its free water. These 4 gases are follows- CO 2 > O 2 > N 2 > H 2
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Conventional amphotericin Amphotericin B deoxycholate (Fungizone, and others), the old non-lipid formulation of amphotericin, is by far the least expensive, but the developmen
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