What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Calcium channel blockers, Calcium antagonists are not recommended for the t...

Calcium antagonists are not recommended for the treatment of CHF because of their negative inotropic effects. However, second-generation dihydropyridine-type calcium antagonists su

Explain empiric initial therapy, Empiric Initial Therapy  Until suscept...

Empiric Initial Therapy  Until susceptibility results are available, empiric initial treatment consists of a 4-drug regimen of isoniazid, rifampin, pyrazinamide and ethambutol.

Basic research terms - nursing research, Basic Research Terms: Some of...

Basic Research Terms: Some of the basic  terms that are defined  in  this section are:  Assumptions Operational Definitions  Variables  Delimitation and Limitation  H

Types of mechanism - stress condition, Types of Mechanism - Stress Conditio...

Types of Mechanism - Stress Condition Plants have evolved a variety of mechanisms to withstand stress conditions. In many cases we can precisely define the way plants enable t

Zoology, Is viruses classified as living organism? If yes/no why

Is viruses classified as living organism? If yes/no why

Define molisch's test or alpha naphthol reaction, Define Molisch's Test or ...

Define Molisch's Test or alpha naphthol reaction? The Molisch test is a common test for the existence of carbohydrates. Principle The reaction is due to the formation of

Common causes related with angina pectoris, Q. Common causes related with a...

Q. Common causes related with angina pectoris? • The usual cause of angina is the narrowing of the major coronary artery due to atherosclerosis. • Systemic hypertension inc

Chondroitin sulphate, CHONDROITI N SULPHATE It is a linear polymer of ...

CHONDROITI N SULPHATE It is a linear polymer of sulphated N-acetylgalactosamine alternating with glucuronic or iduronic acid. The complex also occurs in skin, tendon and ca

Explain the aseptic technique - sub culturing, Explain the Aseptic Techniqu...

Explain the Aseptic Technique - Sub Culturing? Aseptic technique is a series of procedures used to prevent contamination of cultures and sterile culture media. It is one of the

Explain endonucleases, Explain Endonucleases Endonucleases  are  nucle...

Explain Endonucleases Endonucleases  are  nucleases present  in  the pancreatic juice like RNAase  andDNAase. These hydrolyze internal phosphodiester bonds which are located m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd