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What is PCR? How does PCR works?
PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.
First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.
Determine Protein requirements of school children and adolescents? FAO/WHO/UIW (1985) recommends factorial method to compute their protein requirements. The factorial value is
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Define other consideration for Nutrient Requirement and Dietary Management Oral intake is generally feasible only during the anabolic phase wherein the patient should be given
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Describe how a phagocyte destroys bacteria. The phagocyte forms a pouch in its cell membrane and engulfs bacteria in the pouch. It then pinches off the pouch to produce a vesi
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Beta-blockers have traditionally been considered contraindicated in patients with heart failure because they may block the compensatory actions of the sympathetic nervous system wi
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Types of Parthenocarpy Three types of Parthenocarpy are generally recognized: Genetical Environmental, Chemically-induced. Genetical Parthe
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