What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Normal color vision- color blindness, If a color blind man marries a woman ...

If a color blind man marries a woman pure for normal color vision, it's probable that one of the below situations may result. Is it possible that a) All the children would be

What is the main biological process, Q. What is the main biological process...

Q. What is the main biological process that consumes carbon dioxide? The major biological process that consumes carbon dioxide is photosynthesis.

Explain the endosperm of angiosperms, How different are the endosperm of gy...

How different are the endosperm of gymnosperms and the endosperm of angiosperms? In gymnosperms the endosperm is haploid (n), it is known as primary endosperm. In angiosperm th

Observation of bumpus, An interesting observation made by an American biolo...

An interesting observation made by an American biologist H.C. Bumpus (1899) provides a good explanation for normalising selection. Bumpus collected some 136 injured house sparrows

Explain the endochondral ossification, Explain the endochondral ossificatio...

Explain the endochondral ossification The second type of osteogenesis is endochondral ossification. This process is different from intramembranous ossification in that it occur

Use of the activity chart, Use of the Activity Chart Organizing t...

Use of the Activity Chart Organizing teams and to decide its strength of different operations on mass production work. Establishing teams for maintenance and similar

Explain defensive proteins, Explain Defensive Proteins These proteins p...

Explain Defensive Proteins These proteins protect against diseases. Antibodies are the example of defensive proteins. These  combat bacteria and viruses. Also, immunoglobulins,

What is hemolytic disease of newborn, Questiion 1 List various methods ...

Questiion 1 List various methods used for hemoglobin estimation of donor. Add a note on specific gravity method Question 2 Why is it important to follow safety measures

Synthesis and storage of hormone, Synthesis and Storage of Hormone The...

Synthesis and Storage of Hormone The synthesis and storage of hormone depends on the nature of the hormone. Steroid hormones are secreted in diffuse molecular form and usually

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd