What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

What volume of water to make solution, You need to prepare 500 ml of a solu...

You need to prepare 500 ml of a solution containing 10 mM Tris, 0.15 M NaCl and 1 mg/ml SDS. At your work disposal are stock solutions containing 1 M Tris, 2.5M NaCl and 10% (w/v)

What is lymphatic system in human biology, What is Lymphatic System in huma...

What is Lymphatic System in human biology? The lymphatic system maintains fluid balance and serves in protecting the body from disease. The primary organs of the lymphatic syst

Sausage manufacture - meat products, Sa u s a g e manufacture Saus...

Sa u s a g e manufacture Sausage enjoys a prime place among various meat products. Major advancements in processing technology (rapid curing, hot processing, use of solubl

Pathogenesis, Even though association between GAS pharyngitis and the ARF i...

Even though association between GAS pharyngitis and the ARF is fairly well established, the exact pathogenic mechanisms are not clearly understood. However, two mechanisms are post

What are organic compounds in the cytoplasm, High salt concentrations can b...

High salt concentrations can be toxic to plants as they have the potential to disrupt essential cell functions. Yet many halophytes sequester high amount of salt in their vacuoles,

Observation of bumpus, An interesting observation made by an American biolo...

An interesting observation made by an American biologist H.C. Bumpus (1899) provides a good explanation for normalising selection. Bumpus collected some 136 injured house sparrows

Embyonic development, What are the different patterns of cleavage (segmenta...

What are the different patterns of cleavage (segmentation of fertilized egg cell)?

What do you mean by herbaria and museums, Q. What do you mean by Herbaria a...

Q. What do you mean by Herbaria and Museums? A herbarium is a collection of pressed and dried plants arranged according to some valid system of classification and available for

Explain animal fats, Animal Fats This group consists of depot fats from...

Animal Fats This group consists of depot fats from domestic land animals (e.g., lard and tallow), all containing large amounts of C16 and C18 fatty acids, medium amounts of uns

Explain the acoelomates - animals without a body cavity, Explain the Acoelo...

Explain the Acoelomates - Animals without a Body Cavity? The simplest group of animals that has bilateral symmetry and a solid body (acoelomate) is the Platyhelminthes. Phy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd