What is pcr, Biology

Assignment Help:

What is PCR? How does PCR works?

PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor, Kary Mullis, won the Nobel prize for Chemistry in 1993.

First, the DNA to be tested is heated to cause the double helix to rupture and the polynucleotide chains to be exposed. Then small synthetic sequences of DNA called as primers and containing nucleotide sequences similar to the sequences of the extremities of the region to be studied (for example, a region containing a called gene exclusive of a given organism) are added. The primers paired with the original DNA in the extremities of the gene to be amplified. Enzymes called as polymerases, that catalyze DNA replication, and nucleotide supply are added. The primers then are completed and the chosen region is replicated. In the presence of more primers and more nucleotides millions of copies of that specific region are produced. (PCR is very sensitive even using a minimal amount of DN.

 


Related Discussions:- What is pcr

Products of hemp plant, PRODUCT S OF HEMP PLANT - Four drugs - bhan...

PRODUCT S OF HEMP PLANT - Four drugs - bhang, ganja, charas & marijuana obtained from Cannabis indica and C. sativa of family Moraceae . Main alkaloid is tetrah

What elements are present in carbohydrates, (a) Name four examples of compo...

(a) Name four examples of compounds which are classed as carbohydrate. (b) What elements are present in carbohydrates? (a) Sugars (glucose, fructose, maltose, sucro

Define a tumbler garden, A tumbler garden Grow several kinds of seeds i...

A tumbler garden Grow several kinds of seeds in 'tumbler gardens'. Every pupil might grow a tumbler garden of his own and keep a day by day pictorial record of the progress of

What is infra-diaphragmatic type of tapvc, What is Infra-diaphragmatic Type...

What is Infra-diaphragmatic Type of TAPVC? Initial stages of operation are the same as for supracardiac type of TAPVC. The heart is lifted out of the pericardial cavity and c

Embyonic development, What are the different patterns of cleavage (segmenta...

What are the different patterns of cleavage (segmentation of fertilized egg cell)?

Genetics, what is a homologous chromosome

what is a homologous chromosome

HEALTHY DIET, EXPLAIN THE CONSTITUENT OF FOOD GROUPS AND THEIR SOURCES

EXPLAIN THE CONSTITUENT OF FOOD GROUPS AND THEIR SOURCES

Flying fish pisces, the characteristics of flying fish belong to pisces

the characteristics of flying fish belong to pisces

Classic procedure- mitral valve replacement, Classic Procedure: The app...

Classic Procedure: The approach is the same as described earlier for open mitral valvotomy. The excision starts with an anterior incision on the anterior leaflet at 12o'clock p

Character of phylum annelida, what are the advanced characters of phylum an...

what are the advanced characters of phylum annelida that cannot be found in other phyla?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd