Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Q. What is genetic engineering?
Genetic engineering, It is also known as molecular cloning or gene cloning, is artificial recombination of nucleic acid molecules in a test tube; their insertion into a virus, bacterial plasmid or other vector system; and subsequent incorporation of chimeric molecules into a host organism in which they are capable of continued propagation. Construction of such molecules hasalso been called gene manipulation since it usually involves production of novel genetic combinations by biochemical means. Genetic engineering techniques include cell fusion and the use of recombinant DNA or gene-splicing. In cell fusiontough outer membranes of sperm and egg cells are removed by enzymes, and then fragile cells are mixed and combined with aid of chemicals or viruses. Result may be the creation of a new life form from two species (a chimera). RecombinantDNA techniques transfer a specific genetic activity from one organism to the subsequent through the use of bacterial plasmids (small circular pieces of DNA lying outside the main bacterial chromosome) and enzymes, like restriction endonucleases (that cut the DNA strands); reverse transcriptase (that makes a DNA strand from an RNAstrand); DNA ligase (that joins DNA strands together); and Taq polymerase (that can make a double-strand DNA molecule from a single-strand "primer" molecule). Recombinant DNA process begins with the isolation and fragmentation of suitable DNA strands. After these fragments are combined with vectors, they are carried into bacterial cells, where DNA fragments are "spliced" on to plasmid DNA which has been opened up. These hybrid plasmids are then mixed with host cells to form transformed cells. Because only some of the transformed cells will exhibit desired characteristic or gene activity, transformed cells are separated and grown individually in cultures. This methodology has been successful in producing large quantities of hormones (like insulin) for biotechnology industry. However it is more difficult to transform plant and animal cells. Yet technique exists to make plants resistant to diseases and to make animals grow larger. As genetic engineering interferes withprocesses of heredity and can change genetic structure of our own species, there is much concern over ethical ramifications of such power, and possible health and ecological consequences of creation of these bacterial forms.
MN blood group in humans is under the control of two co-dominant alleles M and N. Blood typing results of a population having of 1349 individuals showed that: - 363 persons h
Types of Development of Animals Different animals have evolved various methods of development. These methods can be broadly categorized into two categories (i) Direct devel
The major ecological imbalance on Earth is between the human species and the rest of Earth's biota. What are two specific actions we could do to achieve a balance (for example, rec
Explain the gradient theoryof experimental embryology
Magnesium Magnesium is closely related to calcium and phosphorus in its dispersion and functions in the body. Most of the body’s magnesium is found in bones and muscles. Grass
Armamentarium 1. Denture duplicator or a modified plastic soapdish. (A plastic large soapdish with a base and cover can be adapted by drilling 2 mm holes interspersed and cover
Define the Theory or Principle of Drying? Drying can be illustrated as the application of heat under controlled conditions to eliminate the majority of the water generally avai
You have identified a protein in a cell that you know that comes from the human body. With respect to that protein you have also determined that it allows sodium ions to go through
what is nomenalistic specie concept
Database Search: Once an open reading frame or the partial amino acid sequence has been pre-determined, the investigator compares sequence with others in the databases using a com
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd