Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Q. What is genetic engineering?
Genetic engineering, It is also known as molecular cloning or gene cloning, is artificial recombination of nucleic acid molecules in a test tube; their insertion into a virus, bacterial plasmid or other vector system; and subsequent incorporation of chimeric molecules into a host organism in which they are capable of continued propagation. Construction of such molecules hasalso been called gene manipulation since it usually involves production of novel genetic combinations by biochemical means. Genetic engineering techniques include cell fusion and the use of recombinant DNA or gene-splicing. In cell fusiontough outer membranes of sperm and egg cells are removed by enzymes, and then fragile cells are mixed and combined with aid of chemicals or viruses. Result may be the creation of a new life form from two species (a chimera). RecombinantDNA techniques transfer a specific genetic activity from one organism to the subsequent through the use of bacterial plasmids (small circular pieces of DNA lying outside the main bacterial chromosome) and enzymes, like restriction endonucleases (that cut the DNA strands); reverse transcriptase (that makes a DNA strand from an RNAstrand); DNA ligase (that joins DNA strands together); and Taq polymerase (that can make a double-strand DNA molecule from a single-strand "primer" molecule). Recombinant DNA process begins with the isolation and fragmentation of suitable DNA strands. After these fragments are combined with vectors, they are carried into bacterial cells, where DNA fragments are "spliced" on to plasmid DNA which has been opened up. These hybrid plasmids are then mixed with host cells to form transformed cells. Because only some of the transformed cells will exhibit desired characteristic or gene activity, transformed cells are separated and grown individually in cultures. This methodology has been successful in producing large quantities of hormones (like insulin) for biotechnology industry. However it is more difficult to transform plant and animal cells. Yet technique exists to make plants resistant to diseases and to make animals grow larger. As genetic engineering interferes withprocesses of heredity and can change genetic structure of our own species, there is much concern over ethical ramifications of such power, and possible health and ecological consequences of creation of these bacterial forms.
MCNAUGHTON'S CASE AND LAW: In 1843, McNaughton shot and killed Sir Robert Peel's secretary Edward -Drymmond. It was shown that McNaughton suffered from a number of delusi
Define Principle for Simple Staining of Bacterial Cultures? In simple staining, a single staining agent preferably a basic dye is used. Commonly used basic stains are carbol fu
How does photoperiodism affect the flowering of some plants? The Flowering is an easy and typical to observe example of photoperiodism. Most of flowering plants flower only dur
Question 1 A patient is admitted to the hospital with suspected pneumococcal pneumonia. How would you confirm this initial diagnosis? Answer the following questions a) Which is
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
Maturation Phase The Maturation phase characterizes he transformation of diploid primary spermatocytes into haploid spermatids. The primary spermatocyte divides meiotically t
Indications for Surgery : Once diagnosis of constrictive pericarditis is made and confirmed by chest X-ray, ECG, echocardiogram, CT, MRI scan, cardiac catheterisation and angio,
Q. How different are birds and reptiles concerning the maintenance of body temperature? Are birds rare in polar regions? Reptiles are heterothermic that is they do not control
Explain about the Nucleoproteins? Nucleoproteins are combinations of nucleic acids land simple proteins, which usually consists of a large number of basic amino acids. Nucleopr
A patient has a low red blood cell count, and microscopic examination reveals an abnormally high proportion of circulating reticulocytes. Upon subsequent examination, the patient i
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd