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While various positive control components lie close to the gene they regulate additional can be situated long distances away (sometimes 10-50 kb) either upstream or downstream of the gene shown in the figure. A long-distance positive control series of this type is called as an enhancer if the transcription factor(s) which binds to it enhances the rate of transcription. An enhancer is classically 100-200 bp long and haves several sequence elements which act together to give the whole enhancer activity. When they were first found, enhancers were viewed as a distinct class of control component in which they:
? Over long distances can activate transcription;
? It can be located downstream or upstream of the gene being controlled;
? Are active in moreover orientation with respect to the gene.
Assume that you have 1M stock solutions of NaCl, KCl, and CaCl2. If the ion concentrations in human plasma are 120 mM Na+, 12mM K+, and 2 mM Ca+, compute the volumes of the stock s
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