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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
Explain Hair changes - clinical signs of kwashiorkor? In kwashiorkor, the hair loses its healthy sheen and becomes silkier and thinner. It takes coppery red colour (referred to
Human Development Human development is a continuous procedure that begins when the ovum from a female is fertilised via sperm from a male to form the zygote. Growth and differ
what is the main excretory organ of a lizard
Q. What are the analyses provided by the study of human age pyramids? The study of human age pyramids able to provide the following analyses, proportion of individuals at an ec
Define Principle for Iodine Number Estimation - Lipids? Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative
Why Nutrition is important for health Nutrition is one of the basic components of life. It is an essential part of health care. You already know that good nutrition is essent
Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of
LIPOIDA L MODELS According to Overton (1902) plasma membrane consists of single layer of lipid, because cell permeability is related to lipids. According to Gorter and
Modes of Respiration Many small organisms obtain oxygen by diffusion through their body surfaces. They do not have any specialised respiratory organs nor do they have blood ci
Q. Of what substance is the plant cell wall made? Of which monomer is it made? The plant cell wall is made of cellulose. Cellulose is a polymer whose monomer is glucose. There
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