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A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction of it). A single-stranded cDNA is transformed into double-stranded DNA and inserted into the vector. Every clone in the library is called as a cDNA clone. Unlike a complete genomic library that holds all of the nuclear DNA sequences of an organism a cDNA library have only sequences which are expressed as mRNA. Several tissues of an animal, which express some genes in general but also several different genes, will thus yield different cDNA libraries.
Which are the more abundant ribosomes in secretory cells - the free cytoplasmic ribosomes or those associated with the rough endoplasmic reticulum? Free cytoplasmic ribosomes
Define Precautions for Determination of Inorganic Phosphorus? 1. All reagents should be added in the order mentioned. 2. Allow 10 minutes for the colour to develop in each t
What are peristaltic movements? What is their role in human digestion? Peristalsis is the process of synchronized contractions of the muscular wall of the digestive tube. Peris
Q. Explain about Hypoglycemia? It is a Greek term: hypo -meaning less; glyc- means sweet; and emia- means "of the blood". It is a condition in which less than normal amount of
Spina Bifida Cystica There is a protrusion of meninges and spinal cord a) Meningocele- it is relatively uncommon lesion (4-5 per cent). In this there is protrusion of me
Determine the following term - Amniote egg Eggs that shelter the developing embryo in a water-filled sac—the amnion. Characteristic of the amniote animals, which include reptil
Q. What is the difference between diffusion and osmosis? Osmosis is the phenomenon of movement of solvent particles in general, water from a region of lower solute concentratio
Define the Role of Vitamin D in the immune system? Immune responses that are, mediated by T-cells can be inhibited by tile large doses of calcitriol i.e. 1, 25 dihydroxycholec
Biochemical analysis A basic metabolic panel measures sodium, potassium, chloride, bicarbonate, blood urea nitrogen (BUN), magnesium, creatinine, and glucose. It also sometimes
The internal composition of the cell is maintained because the plasma membrane is selectively permeable to small molecules. A lot of biological molecules are unable to distribute b
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