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What are varices? Why are they more common in the inferior limbs?
Varix means abnormal enlargement of veins. Varices happen when excessive pressure against the normal blood flux makes enlargement of the vein and thus insufficient functioning of its valves (venous insufficiency).
Varices are more common in the veins of the inferior limbs as the fluid column above these vessels is higher. This is the reason why people that spend much time standing (e.g., surgeons) are more susceptible to varices.
You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu
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