Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
What are stains?
Microorganisms are difficult to be seen in living state because of their minute, colourless and transparent nature. Moreover, there is limitation of insufficient contrast to bright field microscopy. Therefore, staining is of prime importance to visualize microbial cells and increase their contrast. Different stains can be used to see these tiny creatures. But first, what are stains? Stains are dyes used to increase contrast by their binding to certain parts of cells or cells themselves. Chemically, stains are coloured organic compounds consisting of three portions.
1. Benzene - colourless organic solvent
2. Chromophore - group with conjugated double bonds that impart colour to benzene or to dye
3. Auxochrome - gives property of ionization to the chromogen (Benzene + Chromophore)
Dyes can bind with cells by ionic, covalent or hydrophobic bonding. Though ionic interaction is the most common means of attachment, dyes can also bind through covalent interactions or due to their solubility characteristic, e.g. Schiff's reagent in Feulgen procedure for DNA staining binds covalently to deoxyribose sugars after hydrochloric acid treatments. Similarly, Sudan III stains lipids because of its solubility in lipid. We shall learn about these dyes/ stains in this practical. Let us begin by studying about the types of stains.
Define Skirt Fold Thickness (SPT) Method? Skin fold measurement is the most widely used field method of body composition assessment. The skin fold (SKF) is an indirect measu
What are the main theoretical models that try to explain the formation of the enzyme-substrate complex? There are two major models that explain the formation of the enzyme-subs
Seed Coat Effects In some seeds dormancy is imposed by the presence of the seed coat; if this is removed, the seed germinates. Two possible types of mechanisms could be invol
What are the types of reproduction that occur in fungi? In fungi there are asexual and sexual reproduction. Fungi replicate asexually by fragmentation, gemmation and sporulatio
Q. What do you mean by Cytotaxonomu and biosustematics? Towards the end of the 19 th century and in the early years of the 20th century, botanists were faced with a problem of
Medical and First Aid - Factors Affecting Occupational Health Depending on the type of work the industry must make arrangement for readily available medical facility. A nearby
Define Physical and Physiological Changes? Every stage has its unique requirements due to different changing needs. With respect to nutrition and health, four different basic a
Define in brief about the Golgi bodies In a cell is achieved in a remarkable fashion by the Golgi bodies. The latter receive the newly synthesised proteins from the rough endop
Explain the Parts of the Microscope? Microscope, as you may have noticed in Figure or for that matter even seen in a laboratory, is a metal body composed of a base and an arm t
Define the lens transparency in metabolism of lens. Lens Transparency: a. Transparency depends on avoidance of large transitions of refractory index. This is in other wor
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd