Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
What are stains?
Microorganisms are difficult to be seen in living state because of their minute, colourless and transparent nature. Moreover, there is limitation of insufficient contrast to bright field microscopy. Therefore, staining is of prime importance to visualize microbial cells and increase their contrast. Different stains can be used to see these tiny creatures. But first, what are stains? Stains are dyes used to increase contrast by their binding to certain parts of cells or cells themselves. Chemically, stains are coloured organic compounds consisting of three portions.
1. Benzene - colourless organic solvent
2. Chromophore - group with conjugated double bonds that impart colour to benzene or to dye
3. Auxochrome - gives property of ionization to the chromogen (Benzene + Chromophore)
Dyes can bind with cells by ionic, covalent or hydrophobic bonding. Though ionic interaction is the most common means of attachment, dyes can also bind through covalent interactions or due to their solubility characteristic, e.g. Schiff's reagent in Feulgen procedure for DNA staining binds covalently to deoxyribose sugars after hydrochloric acid treatments. Similarly, Sudan III stains lipids because of its solubility in lipid. We shall learn about these dyes/ stains in this practical. Let us begin by studying about the types of stains.
What are histogenesis and organogenesis? Histogenesis is the procedure of tissue formation in the embryonic development. Organogenesis is the process of organ formation. Before
what are the phase of alpha taxonomy?
Facilitation Model - Models of Succession This is considered as the classical model of succession. It is based on the assumption that species of a previous stage are replaced
PHYLUM PROTOZOA Definition and Introduction All unicellular ( or acellular ) eukaryotic animals. Most primitive (Gr. Protos = first=zoon= animals ) organisms
Q. Atrial Fibrillation or Flutter? Transient atrial fibrillation or flutter is seen frequently and can be associated with CAD, rheumatic heart disease, thyrotoxicosis, or myoc
Define the Community ecology of disease? Lyme disease is a debilitating infectious disease which has emerged in North America in the last two decades in the landscape context o
There are different types of insulin available and prescribed by the doctor according to the need of the patient. Insulin is grouped according to the speed of action in the body i.
Explain some important phospholipids The chemical structure of phosphatidylcholine (lecithin) illustrated here in Figure is typical of the phosphatides found in the brai
Disorder of Parathyroid Function: The disorders of parathyroid function may either lead to increased production or decreased production of parathormone. Hypoparathyroid
What is the difference between octopuses and mussels regarding their circulatory systems? How does that difference influence the mobility of these animals? Cephalopod molluscs,
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd