What are pure culture techniques, Biology

Assignment Help:

What are Pure Culture Techniques?

Microorganisms can be isolated from various natural environments like soil, food, water, sewage, decomposing matter and even from infectious materials such as pus, sputum, urine etc. These sources usually contain a mixed population of microorganisms containing several species in close association with one another. This mixed population, however, pose a problem for microbiologists as a pure culture (a culture containing only a single kind of microorganisms) is required for studying a particular species.

Different characteristics of a microbe like colony appearance, morphological, physiological, biochemical and immunological characteristics or antimicrobial susceptibility can be studied adequately only in a pure culture. Pure culture can be defined as a population of cells arising from a single cell. Both conventional and more advanced approaches can be used to prepare pure cultures. These are - (1) Plating Method, (2) Agar Shake Tube Method, (3) Most Probable Number (MPN) Method, and (4) Laser Tweezer Technology.

All these methods involve two operations:

(i) isolation of a particular microorganism from a mixed population, and

(ii) cultivation of the isolated microorganism on culture media. Let us get to know about these methods. We begin with the most common one - the plating method.


Related Discussions:- What are pure culture techniques

Develop a hypothesis relating to the amount, Develop a hypothesis relating ...

Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water.

Sketch cis-trans isomer of the first molecule, Please help with the followi...

Please help with the following: a) Draw a diagram (showing all the C and H atoms, and their bonds) for a hydrocarbon with 5 carbons with a double bond between carbons 2 and 3.

Coelenterata – General characters and classification., Coelenterata – Gener...

Coelenterata – General characters and classification.

Define frequency of the dominant allele in this population, Industrial mela...

Industrial melanism refers to the dark pigmentation that evolved in some insects giving them protective coloration on vegetation darkened by soot in heavily industrialized areas pr

Define about the polyphenols, Define about the Polyphenols? We now come...

Define about the Polyphenols? We now come to a group of compounds, which were until recently considered to adversely influence nutrient absorption or giving colour to foods e.g

Circulatory system in cockroach, CIRCUL A T O R Y SYSTEM IN COCKROACH ...

CIRCUL A T O R Y SYSTEM IN COCKROACH Type is open or lacunar. i.e. b.v. absent. Organs are dipped in haemolymph. 1 .      HAEMOLYMPH - Blood and lymph are mixed.

Determine the genotypes of parents, In humans, the recessive allele that ca...

In humans, the recessive allele that causes a form of red-green colour blindness (c) is found on the X chromosome. a) Determine the genotypes and phenotypes of the F1 generation fr

How can the formation of egg cells from germ cells, Indicating the name and...

Indicating the name and respective ploidy of each involved cell how can the formation of egg cells from germ cells be described? The formation of egg cells starts with a germ c

Tetrasaccharides, TETRASACCHARIDES The oligosaccharides are formed b...

TETRASACCHARIDES The oligosaccharides are formed by condensation of four monosaccharides. T rachyos e is a nonreducing tetrasaccharide found in plants as transport ca

Waste management in other species, W a s t e Management in other specie...

W a s t e Management in other species Solid and pellet nature of sheep and goat faecal matter make it easier to handle. This manure can easily be swept off the floors and d

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd