What are pure culture techniques, Biology

Assignment Help:

What are Pure Culture Techniques?

Microorganisms can be isolated from various natural environments like soil, food, water, sewage, decomposing matter and even from infectious materials such as pus, sputum, urine etc. These sources usually contain a mixed population of microorganisms containing several species in close association with one another. This mixed population, however, pose a problem for microbiologists as a pure culture (a culture containing only a single kind of microorganisms) is required for studying a particular species.

Different characteristics of a microbe like colony appearance, morphological, physiological, biochemical and immunological characteristics or antimicrobial susceptibility can be studied adequately only in a pure culture. Pure culture can be defined as a population of cells arising from a single cell. Both conventional and more advanced approaches can be used to prepare pure cultures. These are - (1) Plating Method, (2) Agar Shake Tube Method, (3) Most Probable Number (MPN) Method, and (4) Laser Tweezer Technology.

All these methods involve two operations:

(i) isolation of a particular microorganism from a mixed population, and

(ii) cultivation of the isolated microorganism on culture media. Let us get to know about these methods. We begin with the most common one - the plating method.


Related Discussions:- What are pure culture techniques

#title, IN WHAT ASPECT ARE CNIDARIANS SIMILAR TO PROTOZOANS

IN WHAT ASPECT ARE CNIDARIANS SIMILAR TO PROTOZOANS

About JACK FRUIT, How is the development of JACK FUIT fruit takes place fr...

How is the development of JACK FUIT fruit takes place from seed to fruit?

Insulators - transgenic animals, Insulators Numerous experiments showed...

Insulators Numerous experiments showed that a transgene is generally poorly expressed when it contains a cDNA rather than the corresponding genomic DNA; when it is integrated a

Imaginal discs, Imaginal Discs In the holometabolous larva, there are ...

Imaginal Discs In the holometabolous larva, there are two cell populations: (1) The larval cells that are used for the larval structures and (2) The imaginal disc and th

Altitudinal variations, Altitudinal Variations We know that temperature...

Altitudinal Variations We know that temperature decreases with increasing altitude. This is mainly due to convection currents in the troposphere - the lowermost (and most dense

Class of subphylum uniramia - symphyla, Class of Subphylum Uniramia - Symph...

Class of Subphylum Uniramia - Symphyla Symphyla is yet other small myriapodous group that includes around 160 described species. These are also soil living forms and live in l

Explain chemical bonds in basic chemistry, Explain chemical bonds in basic ...

Explain chemical bonds in basic chemistry? Chemical Bonds :  Chemical bonds are forces that join atoms together in a functional unit. There are three types of bonds that can

What to do to speed up the reaction, Sucrose, or table sugar, can react wit...

Sucrose, or table sugar, can react with water to produce two other compounds, glucose and fructose. Though, when you add sugar to a glass of water, this reaction proceeds ext

Explain about the rna viruses - carcinogenic, Explain about the RNA Viruses...

Explain about the RNA Viruses - carcinogenic? All oncogenic RNA viruses are retroviruses. They are of 2 types. They are acute transforming retroviruses and slow transforming re

How can a child become infected with gonorrhoea, How can a baby become infe...

How can a baby become infected with (a) gonorrhoea, (b) syphilis   (a)  During birth, a baby might be become infected with gonorrhoea bacteria as it passes by t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd