What are pure culture techniques, Biology

Assignment Help:

What are Pure Culture Techniques?

Microorganisms can be isolated from various natural environments like soil, food, water, sewage, decomposing matter and even from infectious materials such as pus, sputum, urine etc. These sources usually contain a mixed population of microorganisms containing several species in close association with one another. This mixed population, however, pose a problem for microbiologists as a pure culture (a culture containing only a single kind of microorganisms) is required for studying a particular species.

Different characteristics of a microbe like colony appearance, morphological, physiological, biochemical and immunological characteristics or antimicrobial susceptibility can be studied adequately only in a pure culture. Pure culture can be defined as a population of cells arising from a single cell. Both conventional and more advanced approaches can be used to prepare pure cultures. These are - (1) Plating Method, (2) Agar Shake Tube Method, (3) Most Probable Number (MPN) Method, and (4) Laser Tweezer Technology.

All these methods involve two operations:

(i) isolation of a particular microorganism from a mixed population, and

(ii) cultivation of the isolated microorganism on culture media. Let us get to know about these methods. We begin with the most common one - the plating method.


Related Discussions:- What are pure culture techniques

Explain the resting membrane voltage, At 1 AM, a researcher places a health...

At 1 AM, a researcher places a healthy squid giant axon in a bath of normal squid physiological extracellular saline and internally perfuses the axon with normal squid intracellula

What are the results of atrial septal defect, What are the results of Atria...

What are the results of Atrial Septal Defect ? Mortality for ASD closure has approached 0 per cent in most cardiac surgery centers. Late survival of patients who has ASD closu

Explain the goal of bcc for a diabetic patient, The overall goal of BCC pro...

The overall goal of BCC programs for diabetes mellitus is to promote behaviors that control diabetes mellitus and prevent complications. These include: Following treatment

Protozoans, Protozoans Prokaryotes represent only a few thousand of th...

Protozoans Prokaryotes represent only a few thousand of the estimated 2 million species of living beings known on the earth today. The rest are mostly eukaryotes that form the

Ground water sources and characteristics, After glaciers, ice caps and snow...

After glaciers, ice caps and snowfields, ground water is the next largest fresh water reservoir. Precipitation that does not evaporate back into the air and does not run off over t

Psychedelic drugs (hallucinogens), PSYCHEDELI C DRUGS (HALLUCINOGENS) - ...

PSYCHEDELI C DRUGS (HALLUCINOGENS) - They act mainly on CNS and greatly alter one's thoughts, feelings and perceptions, called psychedelic drugs. They cause optical or a

Budding- asexual reproduction, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Composite endosperm - variants of endosperm, Composite Endosperm - Variants...

Composite Endosperm - Variants of Endosperm In the family Loranthaceae, the development of endosperm is unique. The ovary lacks ovules. The sporogenous tissue located at the b

What are the substances transferred from the mother to fetus, What are the ...

What are the main substances transferred from the mother to the fetus through the placenta? And from the fetus to the mother? From the mother to the fetus the major transferre

Describe shunts, Q. Describe Shunts? Detection, localization and quanti...

Q. Describe Shunts? Detection, localization and quantification of intracardiac shunts are one of the most important exercises in cardiac catheterization. In most cases a prelim

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd