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What are main limitations on size that can be synthesized and how will that be change over next few years?
A: Today, a 10kb gene or longer is built as part of the regular production method whilst its synthesis had been really challenging ten years ago. Standard processes cover up to 60kb now and what we can do simply in our R&D labs is 100kb. Indeed, the main limitations on size come from E. coli as the DNA propagation host. When the size gets important compared to its own genome, E. coli gets into problems. This can be overcome by switching to other suited hosts, e. g. like yeast. We expect size limitations to further reduce in the future and expect mega base sizes to be more or less easy to get synthesized within the upcoming years.
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