Types of chromatography- purification of organic compounds, Chemistry

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Types of chromatography- Purification of organic compounds

Depending on the nature of the stationary and the mobile phases, the different types of chromatographic methods generally used are in a given table,

Table

Type of Chromatography
Mobile/Stationary Phase
Uses

Column chromatography or adsorption

Liquid/Solid

Large scale separations

Thin-layer chromatography 

Liquid/Solid

Qualitative analysis (identification and characterization of organic compounds)

High performance liquid chromatography

Liquid/Solid

Qualitative and quantitative analysis

Gas-liquid chromatography (GLC)

Gas/Liquid

Qualitative and quantitative analysis

Partition chromatography or ascending paper chromatography

Liquid/Liquid

Qualitative and quantitative analysis of polar organic compounds (sugars, α-amino acids and inorganic compounds)

It is constant for a specific substance (component) within a given set of conditions. Hence, it is possible to identify the several components by determining their Rf values.


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