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Tricuspid Valve Replacement : It is done for organic tricuspid valve disease and at times for functional tricuspid I regurgitation. When done along with other procedures, it is done last when patient is being rewarmed. The advantage is that one can do it after removing aortic cross clamp and on a beating heart, thereby reducing cardiac ischaemic time. Valve is excised leaving 2-3 mms of cuspal tissue. Interrupted, pledgetted mattress sutures are taken with special care at the region of the penetrating bundle of his. Continuous prolene sutures can also be used for valve replacement.
Techniques of Plant Tissue Culture A standard tissue culture laboratory should provide facilities for washing and storage of glass ware, preparation and storage of nutrient
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