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In E. coli there are seven rRNA transcription units scattered by the genome, each of that contains one copy of every of the 16S, 23S and 5S rRNA genes and one to four copies of several tRNA genes which is shown in the diagram. This gene assembly is transcribed by the single prokaryotic RNA polymerase to yield a one 30S pre-rRNA transcript about 6000 nt in the size. This arrangement make sures that stoichiometric amounts of the several rRNAs are synthesized for ribosome assembly. Subsequently transcription, the 30S pre-rRNA molecule forms internal base paired regions to provide a series of stem-loop structures and ribosomal proteins bind to form a ribonucleoprotein (RNP) difficult.
Figure: Transcription and processing of prokaryotic rRNA.
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notochotr is absent in the group ...........
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write a note on clonning
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