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Transcribed regions
The transcribed regions of the genes contain a number of regulatory elements that control exon splicing, mRNA transfer to cytoplasm translation, mRNA targeting, and mRNA half-life. A vector for transgene expression must contain at least one intron, which is required for the transfer of the mRNA to cytoplasm, although several introns may be present. The intron may be added before or after the cDNA. Alternative splicing is a quite frequent event in higher-vertebrate mRNA to control gene expression and to enhance the diversity of the cellular mechanisms using a limited number of genes. The association of introns and cDNAs often leads to alternative splicing, which prevents the transgene from driving the synthesis of the expected protein. A model gene construct using whey acid protein promoter is shown below:
Fig. WAP (whey acidic protein) construct for expression in mammary gland.
Following are the advantages of the DSME: It enables the patient to 1. Accept the disease. 2. Gain knowledge about disease, its prevention, treatment and management. 3.
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What is the need of Monitoring and Surveillance? Monitoring, we know, is the act of observing something and sometimes keeping a .record of it while surveillance is a repeated s
Particulates: These are suspended droplets, solid particles or mixture of two. So all the atmospheric substances that are not gases are called particulates. A number of term
Male reproductive system of frog: i) Testis : a) There are a pair of testis which are attached to the kidneys. b) They are oval and yellow in colour. c) Inside the testis th
The digitalis glycosides are the only orally active positive inotropic agents currently available. The positive inotropic occurs through inhibition of the enzyme Na + -K + -ATPase
What are the excretory organs of crab
Sucrose is commonly used to preserve fruits why not glucose? Ans) Glucose is reactive due to its open-chain form having an aldehyde group.
FUNCTIONS OF E.R. (1 ) Mechanical support - E.R. participates in formation of cytoskeleton with microfilaments, microtubules and intermediate filaments. (2 )
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