biophysics, Biology

Assignment Help:
you are setting up a pcr reaction to amplify a 1000 basepair long dna grament. in your 50 microliter reaction volume you have 100 nanogrM OD RWMPLrw sn QIRH eoufly 50/50 at/gc 100 micro moler reverse primer, 100 micromolar reverse primer, 200 micromole of each of the nucleotides, a sufficient amount of plymerase and bugges.

Is the amount of product dna in this set up limited by the amount of template dna, primers, or nucleotides?

Related Discussions:- biophysics

Photosynthesis, Photosynthesis Two main energy pathways are recognised...

Photosynthesis Two main energy pathways are recognised in living systems. These are photosynthesis and cellular respiration. Both have their respective but different electron

Define visible hazards in food, Normal 0 false false false ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Maintenance and regulation of peripheral circulation, Maintenance and Regul...

Maintenance and Regulation of Peripheral Circulation Pressure receptors (baroreceptors) located in the wall of the internal carotid arteries and in the arch of aorta, when

Atrial fibrillation or flutter, Q. Atrial Fibrillation or Flutter? Tran...

Q. Atrial Fibrillation or Flutter? Transient atrial fibrillation or flutter is seen frequently and can be associated with CAD, rheumatic heart disease, thyrotoxicosis, or myoc

Temperate shrublands, These are areas where woody shrubs predominate rather...

These are areas where woody shrubs predominate rather then trees. In regions with a Mediterranean type of climate i .e., hot dry summers and cool wet winters, shrubs grow close tog

Steps for elongation , a) The elongation level of translation in eukaryo...

a) The elongation level of translation in eukaryotes requires three elongation factors, eEF-IB, eEF-1A and eEF-2, that have similar functions to their prokaryotic counterparts EF-G

Explain about gastro oesophageal reflux disease, Q. Explain about Gastro Oe...

Q. Explain about Gastro Oesophageal Reflux Disease? GERD refers to the regurgitation of acidic stomach contents into the oesophagus. It results in a spectrum of clinical manife

What are the typical fauna of the tropical forests, What are the typical ve...

What are the typical vegetation and the typical fauna of the tropical forests? In the vegetation of the tropical forests broadleaf evergreen trees predominate. On the top of th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd