Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Tissue Culture
It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).
Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.
Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.
Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.
Ribosomes - It is also known as Protein factory, Engine of the cell. Ribosomes are smallest cell organelles (150 x 250 Å). Unit membrane less cell organelle. Ribo
Define the Role of Riboflavin in Respiratory Chain? Riboflavin catalyzes numerous oxidation-reduction reactions. Conversion of riboflavin to flavin mononucleotide (FMN) and the
One may find leucocytosis with predominant polymorphonuclear cellular response in patients of ARF in presence of acute some throat. Throat Culture When patient presents as AR
Is transportation of gases in tracheophytes made through the vascular tissues? Carbon dioxide and oxygen are not transported by the xylem or phloem. These gases reach the cells
Q. Evaluate the texture of various foods? A number of instruments are available to evaluate the texture of various foods. Brief discussions on these instruments follow. Cons
Q. How is it explained that a person with the spinal cord sectioned at the cervical level is still able to perform the patellar reflex? The arch reflex depends only on the inte
THEORIES TO EXPLAIN FINE STRUCTURE OF CHROMOSOME (1 ) Multistranded theory Steffenson 1959 - each chromosome composed of two chromatids (Half Chromosome) and each chromat
Partial Pericardiectomy : The approach can be either by a left anterolateral thoracotomy or median sternotomy. For pyogenic pericarditis with adhesions and loculations a par
Explain about the Oral cavity and alimental-y tract? Various functional changes and decline in secretary function occur in the digestive tract with aging. These include: Or
Structural Features of Viruses Size of Viruses Virus particles vary widely in size. The smallest viruses e.g. virus of foot and mouth disease measuring about 20 mill mic
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd