Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Tissue Culture
It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).
Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.
Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.
Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.
Lorica - body form Protozoans It is a covering which unlike the theca fits loosely around the organism. It is a cup, bell or vase-like structure with one opening through which
Theoretical Foundation of Halsted Reitan battery There are really two theoretical bases for the Halsted Reitan battery, one contained in brain and intelligence and related writ
how much classification of protozoa
Q. In which part of the digestive tube is water is chiefly absorbed? What about the mineral ions and vitamins? Most part of water, mineral and vitamins ions are absorbed by the
Q. Indicating the name and respective ploidy of each involved cell how can the formation of egg cells from germ cells be described? The formation of egg cells begins with a ger
Ask question #i need the complete table having the names of the diseases their causative agents and their control,caused by air-borne pathogens?
Rituals: The social life of the earliest human groups or tribes revolved around food gathering. To begin with, they must have collected anything they could eat-seeds, nuts, f
Subtropical rain forests - Ecosystem In regions of fairly high rainfall but less temperature differences between winter and summer and broad-leaved evergreen subtropical biome
Determine the groups of soil organisms The soil organisms are classified into two broad groups viz. soil flora (related to plants) and soil fauna (related to animals) which ar
Define Goals of National and International Requirement Estimates and RDAs? One of the goals of the RDAs has been reiterated through this unit. They serve as the guidelines for
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd