Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Tissue Culture
It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).
Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.
Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.
Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.
TYPES OF DRUG ACTION - 1 . PHARMACODYNAMIC DRUGS - These drugs modify the physiological and biochemical processes in the patient's body to eliminate the disease.
what is steroid production by microbial metabolism
Pigs This is a sub-acute or chronic infection manifested by abortion, sterility, high piglet mortality and orchitis in pigs. Br. suis causes brucellosis among pigs. It is morph
Q What is the genetic material of a virus? How does that material act in viral reproduction? There is DNA viruses double strand or single strand DNA and RNA viruses double stra
Pre Pregnancy Counselling To prevent excess spontaneous abortions and congenital malformations in infants of diabetic mothers, diabetic care, education and counselling must be
Q. Can you explain Restrictive Ventricular Septal Defect ? VSD is restrictive when VSD pressure gradient is more than 60mmHg (systolic cuff pressure-VSD lpressure grad
At 1 AM, a researcher places a healthy squid giant axon in a bath of normal squid physiological extracellular saline and internally perfuses the axon with normal squid intracellula
Why Water and Electrolytes are important for exercise? Water and electrolytes are important for exercise performance as they maintain blood volume and osmolality, thermoregulat
Two major objectives must be achieved to treat IE effectively. The infecting micro-organism in the vegetation must be eradicated. Also, invasive, destructive intracardiac and foca
Can you please describe the differences between continuous traits and discrete traits and how it helps an ecologist measure variation? Please give a few examples.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd