Tissue culture, Biology

Assignment Help:

Tissue Culture

It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).

Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.

Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.

Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.

Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.


Related Discussions:- Tissue culture

How is the blood typing concerning the abo system, How is the blood typing ...

How is the blood typing concerning the ABO system and the Rh usually done? In the blood typing for the ABO system and the Rh system a blood sample is collected from the person

List a few applications of starches in the food industry, List a few applic...

List a few applications of starches in the food industry. A few applications of starches in the foods industry include thickener, a fat sparing agent, adhesive, binder,

Explain the sex composition of the population, Explain the Sex Composition ...

Explain the Sex Composition of the Population? Sex composition of human population is one of the basic demographic characteristics, which is extremely vital for any meaningful

Chemical bonding, Chemical bonding : The present matter of earth contains ...

Chemical bonding : The present matter of earth contains atoms bound in groups of two or more called molecules. Their binding capacity is called chemical reactivity; It depends upo

Adult circulation and the human fetal circulation, Q. Concerning the mixtur...

Q. Concerning the mixture of arterial with venous blood what is the difference between the adult circulation and the human fetal circulation? In the human fetal circulation the

Explain the term- ecology, What is Ecology? Ecology is the field of Bio...

What is Ecology? Ecology is the field of Biology that studies the relationship among living beings and among living beings and the environment.

State the enzymes utilization in brewing industry, Enzymes utilization in B...

Enzymes utilization in Brewing Industry Here, the main starting material is malt, produced by allowing barley seeds to germinate under moist conditions. The reserve starch is b

Which of substance are microfilaments, Q. Which Of substance are microfilam...

Q. Which Of substance are microfilaments made and what are the properties of these elements that give motility to cells? Microfilaments are made of actin a protein. The contrac

Why are trees green, Why are trees green? It is because leaves have chl...

Why are trees green? It is because leaves have chloroplasts, which have a green pigment, which also have chlorophyll in them. They are used for conducting photosynthesis, which

Diagnosis, The symptoms and signs of endocarditis are often constitutional ...

The symptoms and signs of endocarditis are often constitutional and, when localized, often result from a complication of IE rather than reflect the intracardiac infection itself. C

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd