Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Tissue Culture
It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).
Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.
Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.
Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.
Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-
Osmoregulatory Animal An osmoregulatory animal is generally in an osmotic steady state even though there may be hourly and daily variations in osmotic balance. The concentrati
ABSORPTIO N OF FOOD Absorption from Mouth Here negligible tobacco, alkohal and some medicines as isoprenailne glycerol tri nitrite is absorbed. Absorptio n from Sto
Changes in Animal Life during Xerosere Just like the hydrosere, there occur successive changes in animal life during the xerosere. A few mites are usually found associated wi
Class of Subphylum Uniramia - Chilopoda Chilopoda have the centipedes. The class contains some 2500 species that have been described so far. They inhabit both of the tropical
I need to come up with a thesis topic for a 8-12 paper. It can be on anything related to Human Biology and I am struggling to come up with a topic/thesis statement. Any help will b
What is the RQ if glucose was the only source of fuel used in metabolism?
Define Field Test used to Assess Aerobic Capacity? A field test that has been used extensively in nutrition studies to assess aerobic capacity measures Heart Rate responses to
What is Mendel's second law? Mendel's second law postulates that two or more different traits are also conditioned by two or more pair of dissimilar factors and that each inhe
Explain Interactions of Macrominerals With Other Nutrients? Various nutrients interact with minerals thereby affecting their bioavailability. These interactions occur at differ
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd