Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Tissue Culture
It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).
Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.
Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.
Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.
Which law of thermodynamics explains the fact that we must eat to gain the energy to perform the functions of life, such as breathing? a. fourth law b. first law c. second law d
Explain Procedure for Preparation of Bacterial Smear? Now carry out the exercise using the steps enumerated herewith: 1. Collect swab samples from the suitable sampling site
Q. Besides the pancreatic juice in the intestine there is the releasing of the enteric juice that contains digestive enzymes too. What are these enzymes and which type of molecule
Fibroblast is the term applied to a cell of connective tissue which is separated from similar cells by some degree of the matrix material; fibroblasts secrete elastin and collagen
MOLECULAR MECHANISM OF HORMONE ACTION - Once a hormone enters the bloodstream it can reach almost any cell in the body. However, each hormone affects only certain kinds of c
In about 80 per cent of ARF patients, ASO titre is significantly raised. ASO titres vary with age, geographical area and other fevers, which influence frequency of streptococcal in
write down the division of cryptogamae and phanerogamae
How many different molecules composed of (A) two (B) three, and (C) four amino acids, linked together by peptide bonds, can be made from the set of 20 naturally occurring amino aci
Problems of Osmoregulation The water, some inorganic salts, nutrient molecules are some of the important components of the body fluids. It is essential for the animals to main
Define Urinary Excretion Test - Thiamin Status? The urinary excretion of thiamin is an index of recent dietary intake of the vitamin. Hence, determination of thiamin in urine s
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd