Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Tissue Culture
It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).
Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.
Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.
Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.
Following ways can be used for control of noise pollution: It can be controlled by designing, fabricating and using quiter machines. In heavy industries, sound proof insul
What roles do membrane proteins play in transporting only certain substances into a cell? Some proteins form channels or pores by which certain substances can pass. Other pr
Q. What are some diseases caused by abnormal GH secretion by the hypophysis? In childhood deficient GH secretion may lead to delayed growth and in severe cases to nanism that i
Contamination of the Implant Body before Insertion The implant may be contaminated by manufacturing error, by the operator, from non titanium instrumentation and by the bacteri
Adverse Effects of Famciclovir Famciclovir has been generally well tolerated. Headache, nausea and diarrhea have been reported. Like acyclovir, famciclovir has been associated
Extracted chlorophyll pigments If we take chlorophyll molecule in a test tube and provide 2 CO and water and expose the mixture to sunlight, glucose is not formed. If we
Process of Memory: There are three stages of memory Encoding process: It is the process of receiving sensory input and transforming it into a form or code, which can be stored.
Clinical Neuropsychology Clinical neuropsychology is the application of neuropsychological knowledge to the assessment, management and rehabilitation of people who have suffere
Planning the Nursing Care Provide bed rest Administer antibiotics as advised Prevent infection Implementation of Nursing Care Provide Bed Rest Sin
Explain disease typhoid Typhoid is often called enteric fever because the infection or bacteria is found in the intestines and attaches itself to the epithelium of the intes
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd