Tissue culture, Biology

Assignment Help:

Tissue Culture

It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).

Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.

Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.

Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.

Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.


Related Discussions:- Tissue culture

What are the consequences of pem, What are the Consequences of PEM? Th...

What are the Consequences of PEM? The consequences of PEM are most often long lasting and irreversible. The common consequences include: • Irreversible growth retardation

Fields in ecology, Ecology is divided into two fields:    (a)Autecology...

Ecology is divided into two fields:    (a)Autecology     (b) Synecology   (a)  Autecology: it deals with life history, population dynamics, behavior etc of a single

Strain of fusarium moniliforme, Q. Strain of Fusarium moniliforme? It w...

Q. Strain of Fusarium moniliforme? It was first obtained from a strain of Fusarium moniliforme isolated from southern leaf blight- damaged corn seed as a water soluble toxin.

Characteristic features of phylum cnidaria, Characteristic features of Phyl...

Characteristic features of Phylum Cnidaria All are aquatic animals. Radial or biradial symmetry around an oro-aboral axis, but no head. Diploblastic, with an epidermi

Do moulds grow better where it is dark or light, Do moulds grow better wher...

Do moulds grow better where it is dark or light? This time leave one culture dish in a warm place where it receives light all the time. Place the other dish in a warm dark plac

Glucose-6-phosphate, Three molecules of ducose-6-phosphate  yield 3 molecul...

Three molecules of ducose-6-phosphate  yield 3 molecules of CO,  and 3 molecules of five carbon residues (pentose sugar). The latter are converted ultimately to  2 molecules  of  g

What are the roles of atp and nadph, Q. What are the roles of ATP and NADPH...

Q. What are the roles of ATP and NADPH in the chemical stage of photosynthesis? NADPH acts as reductant of carbon dioxide it delivers highly energetic hydrogen to precursor mol

What is iodoacetate inhibitor, What is Iodoacetate inhibitor Iodoacetat...

What is Iodoacetate inhibitor Iodoacetate is the inhibitor of glyceraldehyde-3-phosphate  dehydrogenase.

What is dietary management of tuberculosis, Dietary Management It must ...

Dietary Management It must be evident to you that majority of the tuberculosis patients are emancipated and malnourished. Proper dietary management it during and  after the inf

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd