Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Tissue Culture
It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. It is necessary to provide an environment in which cells can live and mulaiply over a periOd of time. Therefore. different media containing mixture of salts, amino acids, vitamins, Wwth factm etc. are used for this purpose. There art some special typeg of cul- media such as Balanced Sak Solution (BSS), and Essential Minimal Medium (EMM).
Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. Then it is cut into small pieces and treated with trypsin. Trypsin has the property of dissociating cell aggregates into a suspension of single cells, with affecting their viability. Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. Such cells show che properties of the parent body frorn which they were obtained, for example, fibrbblasts secrete collagens; cells derived from embryonic skeletal muscle form muscle fibres. These phenomena which otherwise cannot be studied in intact tissues are easily studied by this technique.Some cells show prolonged growth and di~ide~indefinjteilny vitro. These cells are called 'variant cells'. Such cells grow or propagate in a'linear Order indefinitely to form a cell line. Some commonly used cell lines are BHK 21 and Human HeLa cells which are derived from fibroblast of a hamster and epithelial cells of man respectively.
Many of these cell lines were derived from tumor like growth. All these cells are capable of dividing indefinitely in tissue culture and they express at least some of the properties which are different from the cell of origin. BHK 21 cells, and HeLa cells. are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth.
Unlike variant cells, cancer cells divide indefinitely in vivo (within the living organism) as well as in vitro (in the experimental conditions). They grow rapidly compared to normal . cells in a tissue culture dish. Tissue culture is an important technique which is used to study the fundamental problems in cell biology. One of the major advancements in tissue culture is cloning. A clone is a population of cells derived from a single parent cell.
Q. What is bathing in Cerebro-Spinal Fluid? The CNS is bathing in Cerebro-Spinal Fluid (or CSF): similar to plasma, less protein, rich in ascorbic acid, different in ions Na+
Accumulation of phenylalanine and its catabolic products leads to central nervous system damage. The extent of damage caused to the brain depends on the time at which the insult oc
Important aspects related to regulation include: 1) The hormones glucagon and glucocorticoids which are secreted during starvation stimulate glucose-6-phosphatase to en
Female reproductive system of frog: i) Ovaries : a) There are a pair of ovaries present in the abdomen. b) They are grayish or blackish in colour. c) There are numerous cham
EXCRETIO N IN AMOEBA - NH 3 is excreted out through plasmalemma. Osmoregulation takes place by contractile vacoule, generally one, towards posterior end, contractile in
Explain ISP The specification of the Association of American Feed Control Officials, Inc. (AAFCO) defines ISP as "Major proteinaceous fraction of soybeans prepared from dehulle
Which of the following statements about buffers is true? Select one: a. A buffer composed of a weak acid of pKa = 5 is stronger at pH 4 than at pH 6. b. At pH values lower
What are the Preservation of Insulin Regular insulin is stable for 18 months at 23.8 0 C and Lente insulin are stable for 24 months at this temperature. Although refrigeration
What is Energy ? Energy Energy is defined as the capacity to do work. Work is defined as the movement of a mass, or the product of the force and the distance through which t
An element playing significant role in nitrogen fixation is : 1. Molybdenum 2. Copper 3. Manganese 4. Zinc An element playing significant role in nitrogen fixation
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd