Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Establishment of tissue cultures, Establishment of Tissue Cultures By...

Establishment of Tissue Cultures By now you are familiar with the term "Explants". After a few days in culture the explant becomes slightly rough in texture and its surface g

Metabolic processes, Metabolic Processes Living things are complex...

Metabolic Processes Living things are complex and yet, the cell is the basic unit of life New cells result of mitosis cell division DNA controls all cell functions

Dictyosomes, Dictyosomes  are produced on the Organelles in the plant cells...

Dictyosomes  are produced on the Organelles in the plant cells composed of the series of flattened membrane sacs which sort, chemically modify, and package protein srough endoplasm

Explain the sporangiophores features of rhizopus, Explain the Sporangiophor...

Explain the Sporangiophores features of Rhizopus? 1. Sporangiophores swell at the tip into a spherical knob like structure called sporangium. It has two zones. - Central dom

Crustaceans - hormones in growth and reproduction, Crustaceans - Hormones i...

Crustaceans - Hormones in Growth and Reproduction Arthropods have a tough and frequently hard exoskeleton made up of cuticle composed of chitin and protein. The cuticle impose

What is osmosis, What is Osmosis ? Osmosis :  Osmosis refers to the...

What is Osmosis ? Osmosis :  Osmosis refers to the diffusion of water across a semi-permeable membrane from an area of higher concentration of water to an area of lower con

Define osmotic pressure - properties of solutions, Explain Osmotic Pressure...

Explain Osmotic Pressure Osmosis, as you may already know, refers to the flow of solvent into a solution, or from a more dilute solution to a more concentrated solution, when t

Complications of making a definite classification, Assume for this question...

Assume for this question that we are discussing a rare human disorder. Describe as detailed as possible the characteristics of this disorder if it is: autosomal dominant autosomal

What is low - density lipoprotein receptor pathway, Q. What is low - densit...

Q. What is low - density lipoprotein receptor pathway? Ans. The increasing cellular free cholesterol generated regulates the activities of two enzymes that are of crucial

Explain cardiac muscle or heart muscle, Explain Cardiac Muscle or heart mus...

Explain Cardiac Muscle or heart muscle? Cardiac muscle, or heart muscle, is a striated muscle that occurs only in the vertebrate heart. The heartbeat is controlled by noncontr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd