Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Are environmental phenotypical changes, Are environmental phenotypical chan...

Are environmental phenotypical changes transmitted to the offspring? Changes caused on phenotypes by the environment are not transmitted to the offspring (unless their primary

Describe pharmacologic agents in amyl nitrite, Describe Pharmacologic Agent...

Describe Pharmacologic Agents in amyl nitrite ? Amyl Nitrite : It produces vasodilatation which initially results in marked fall in BP and in the next 30-60 seconds there is

Possible recombinant genotypes, A woman is heterozygous for three X-linked ...

A woman is heterozygous for three X-linked loci: AaBbDd. She has seven sons with the following genotypes: two are X ABd Y three are X abD Y one is X ABD Y one is X Abd

Explain horizontal full mucoperiosteal flaps, Explain Horizontal Full Mucop...

Explain Horizontal Full Mucoperiosteal Flaps - Endodontic Surgery      a) Also called:  Envelope flap, b) Only a Horizontal incision, without the vertical releasing incision

Contemporary interests in nutritional biochemistry, Contemporary Interests ...

Contemporary Interests in Nutritional Biochemistry As  newer techniques in biochemistry have emerged from time to time, these were of immense help  in  the  understanding of ut

Biological stress, Biological Stress Since in nature, the various orga...

Biological Stress Since in nature, the various organisms do not live in complete isolation from others, stress to a plant species might also be caused by what other organisms

What is surgical therapy, Surgical Therapy The clinician may use a surg...

Surgical Therapy The clinician may use a surgical approach when non-surgical therapies are not indicated or are unsuccessful. The surgical techniques are modified from those

.Botany, 57. While planning for an artificial hybridisation programme invol...

57. While planning for an artificial hybridisation programme involving dioecious plants, which of the following steps will be irrelevant A usting of pollen on stigma B Collection

Population growth curve, Increase in population size is known as population...

Increase in population size is known as population growth. It depends upon number of persons added to the population and number of persons lost from the population. Addition in pop

Describe arteriovenous continuous murmur, Describe Arteriovenous Continuous...

Describe Arteriovenous Continuous Murmur? Arteriovenous Continuous Murmur :  These can be congenital as in coronary artery fistula entering RA/RV/PA, sinus of valsalva to rig

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd