Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Brodmann areas, One way that the cortex has been parcellated is based on fi...

One way that the cortex has been parcellated is based on fine-grained anatomical differences in the composition of the cortical layers: their thickness and types of neurons that ar

Higher calorific value (hcv) or gross calorific value (gcv), It is defined ...

It is defined as the total amount of heat produced, when unit mass/volume of the fuel has been burnt completely and the products have been cooled to room temperature (~ 15 0 C).

Protozoa, what are the disadvantages of protozoa

what are the disadvantages of protozoa

Is water a polar or a non-polar molecule, Is water a polar or a non-polar m...

Is water a polar or a non-polar molecule? What is the consequence of that characteristic for the function of water as solvent? Water is made of two atoms of hydrogen attached b

What are pure culture techniques, What are Pure Culture Techniques? Mic...

What are Pure Culture Techniques? Microorganisms can be isolated from various natural environments like soil, food, water, sewage, decomposing matter and even from infectious m

Explain the process of absorption of fats, Explain the process of Absorptio...

Explain the process of Absorption of Fats? After digestion, only 25% of triglycerides are broken completely to glycerol and fatty acids. Major digestion product is 2-monoacylgl

Treatment of angina pectoris, Q. Treatment of angina pectoris? Proper a...

Q. Treatment of angina pectoris? Proper and careful treatment of the underlying cause (usually dyslipidemia, advanced atherosclerosis or sever chronic hypertension) is imperati

Role of microorganism in fermentation foods, Normal 0 false f...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

How green leaves make food for plants, Green leaves make food for plants ...

Green leaves make food for plants Heat some alcohol in a jar over boiling water unless it boils. Break various green leaves from a geranium or other plant which has been in the

What are the target organs upon which insulin and glucagon, What are the ta...

What are the target organs upon which insulin and glucagon act? Glucagon mostly acts upon the liver. Insulin acts in general upon all cells. Both also act upon the adipose tiss

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd