Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Explain directive and non-directive counseling, Directive and Non-directive...

Directive and Non-directive Counseling Directive Counseling  tends  to  be appropriate when  the  counselor  is  aware  of  the problem  and/or  is concerned about  the behavio

Name five kingdoms into which living beings are divided, What are the five ...

What are the five kingdoms into which living beings are divided? Which group of living being is out of this classification? The five kingdoms of living beings are the kingdom M

Extracted chlorophyll pigments, Extracted chlorophyll pigments If we...

Extracted chlorophyll pigments If we take chlorophyll molecule in a test tube and provide 2 CO and water and expose the mixture to sunlight, glucose is not formed. If we

Expain kingdom fungi, Kingdom Fungi Fungi was earlier placed with the t...

Kingdom Fungi Fungi was earlier placed with the true plants and are still largely considered so in the domain of botanists yet they are quite different. They are multicellular

Determine the transplantation of mitochondria, Based on your reading of the...

Based on your reading of the article "Hello Mothers, Hello Father" which of the following is a correct statement regarding the transplantation of mitochondria? A. The sperm cel

State the benefit of the new brain imaging technology, Benefit of the new b...

Benefit of the new brain imaging technology Benefit from the new brain imaging technology, notably the CT scan, and the application of high speed computer technologies, permitt

Metaphase chromosomes, Metaphase chromosomes: Each metaphase chromoso...

Metaphase chromosomes: Each metaphase chromosomes is a duplicated structure which consists of two sister chromatids, attached at a point called centromere or primary constric

Explain database search, Database Search: Once an open reading frame or th...

Database Search: Once an open reading frame or the partial amino acid sequence has been pre-determined, the investigator compares sequence with others in the databases using a com

Differences between prokaryotic & eukaryotic cells, DIFFERENCES BETWEEN PRO...

DIFFERENCES BETWEEN PROKARYOTIC & EUKARYOTIC CELLS   S . N o .   CHA R AC T ERS   P R O K AR Y O T I C CELL

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd