Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Name the three main arthropod classes, How are the three main arthropod cla...

How are the three main arthropod classes characterized according to the body division? In crustaceans and arachnids the head is fused with the thorax forming the cephalothorax.

High proportion of reticulocytes, A patient has a low red blood cell count,...

A patient has a low red blood cell count, and microscopic examination reveals an abnormally high proportion of circulating reticulocytes. Upon subsequent examination, the patient i

Show the process of synaptic transmission, Q. Fluoxetine is an antidepressa...

Q. Fluoxetine is an antidepressant drug that presents an action mechanism related to the synaptic transmission. What is that mechanism? Fluoxetine is a substance that inhibits

Define working of kidney in excretion of zinc in humans, Define working of ...

Define working of Kidney in excretion of zinc in humans? Very small amount of zinc is excreted in the urine (0.3-0.7 mg/day), as most of the zinc filtered by the kidney is reab

How to follow a cell through the cell cycle, Follow a cell through the cell...

Follow a cell through the cell cycle. How many copies of each gene are present at each stage?

What is r - wave amplitude, Q. What is R - Wave Amplitude? The R-wave a...

Q. What is R - Wave Amplitude? The R-wave amplitude in the lateral precordial leads usually decreases more in normal than in abnormal subjects and correlates with left ventr

Nutrition, mode of nutrition in tapeworm

mode of nutrition in tapeworm

Autotomy and regeneration, Autotomy and Regeneration Shedding of body...

Autotomy and Regeneration Shedding of body parts in self-defense to avert the attention of the predator-enemy or in any other emergency is a type of autotomy (auto: self, tom

Write the definition of counselling, Q. Write the definition of counselling...

Q. Write the definition of counselling? The counselling process, involves various steps in a sequence as given below: 1. Rapport-Building 2. Locating the Problem 4. Pl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd