Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Define instrument for absorbed radiation - spectrophotometer, Define Instru...

Define Instrument for Absorbed Radiation - Spectrophotometer? In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation

How is the cooling of tissues and organs, Q. How is the cooling of tissues ...

Q. How is the cooling of tissues and organs for medical transplants associated with the effect of temperature upon enzymatic reactions? The molecular degradation during the dec

State the term cercaria, State the term Cercaria? A stage in the life c...

State the term Cercaria? A stage in the life cycle of trematode flukes. Cercaria develops from redia found in intermediate host. This tadpolelike organism is released from inte

Define manganese metabolism - micro minerals, Define Manganese Metabolism -...

Define Manganese Metabolism - Micro Minerals? Intestinal absorption of Mil occurs throughout the length of the small intestine although the exact mechanism of absorption is not

Reproduction, What is the difference between asexual and sexual reproductio...

What is the difference between asexual and sexual reproduction

Explain the term rastelli operations, Explain the term Rastelli operations ...

Explain the term Rastelli operations ? This is the operation of choice for transposition with VSD and left ventricular outflow obstruction. The principle of this operation is:

Saturated fatty acids required for dyslipidemia, Q. Saturated Fatty Acids r...

Q. Saturated Fatty Acids required for dyslipidemia? Saturated Fatty Acids (SFA): These are found mostly in animal fats as white marble-like solid at room temperature. Red meats

Explain pasteurization - method of food preservation, Explain Pasteurizatio...

Explain Pasteurization (temperature below 100° C) - method of food preservation? Pasteurization is a heat treatment that kills a part but not all the microorganisms present and

Darwins theory of natural selection, Darwins theory of natural selection is...

Darwins theory of natural selection is considered a paradigm shift--a theory that has wide-ranging effects. Describe some of the advantages that this theory offered scientists once

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd