Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Classification of carbohydrates - degree of polymerization, Define Classifi...

Define Classification of Carbohydrates - Degree of Polymerization? Classification on the basis of Degree of Polymerization (DP) Carbohydrates are classified by their degree

Introduction to locomotion, LOCOMOTIO N - Displacement of body. Mos...

LOCOMOTIO N - Displacement of body. Mostly animals show locomotion except porifera, urochordata, corals, obelia thier larva may show locomotion. Its importance is -

Equipment of cardio pulmonary bypass , Equipment : To maintain effective c...

Equipment : To maintain effective circulation and carry out functions of the lungs during an open heart operation the equipment should have: 1) Blood Pump 2) Oxygenator 3

Show endocrine glands involved in the menstrual cycle, Q. What are the endo...

Q. What are the endocrine glands involved in the menstrual cycle? What are the hormones in action? The endocrine glands that secrete hormones involved in the menstrual cycle ar

Hydrophilicity plot, Hydrophilicity Plot is a computer plot which checks t...

Hydrophilicity Plot is a computer plot which checks the relative summed hyrophobicity or hydrophilicity of adjacent amino acid sidechains (usually within a moving window of about

Show the communication process steps, Q. Show the Communication process ste...

Q. Show the Communication process steps? Planning - Set goals. - Make a plan with alternatives. - Allow the counsellee to select a alternative which is feasible.

How is excretion done in amphibians, Q. How is excretion done in amphibians...

Q. How is excretion done in amphibians? Adult amphibians have kidneys that filter blood. Nitrogen waste is excreted as urea so amphibians are ureotelic beings. The aquatic, lar

What are the main types of inheritances - epistasis, According to Mendel's ...

According to Mendel's law phenotypical characteristics would be verified by pair of factors (alleles) that separate independently in gametes. What are the main types of inheritance

Valuation of biodiversity, Q. Show the Valuation of Biodiversity? Serio...

Q. Show the Valuation of Biodiversity? Serious research in this field has only been recently initiated and the methodologies for valuation are still evolving. Important valuat

Explain milling extraction rate, Explain Milling extraction rate The ch...

Explain Milling extraction rate The chemical composition of the flour depends on the milling extraction rate.  Increasing the rate of flour extraction decreases the proportion

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd