Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Define about the carbohydrates, Define about the Carbohydrates? In the ...

Define about the Carbohydrates? In the previous unit, we came to know that availability of glucose is the major factor in exercise performance. Hence, carbohydrate manipulation

Dominance-structure of community , Dominance In each community, a few o...

Dominance In each community, a few over topping species are present in greater bulk. By their greater number or biomass (living weight) the dominant species modify the habitat

Which statement about asexual reproduction is false, Which statement below ...

Which statement below about asexual reproduction is FALSE? a.With asexual reproduction, offspring are genetically identical to the parent. b.Asexual reproduction requires no

Determine the stability of natural colorants in foods, Stability of Natural...

Stability of Natural Colorants in Foods Many attempts have been made to retain colouration by adding chemicals or modifying processing conditions. Ascorbic acid has been claime

Nucleic acids, Why is the number of bonds important in nucleic acids

Why is the number of bonds important in nucleic acids

Which are the main positive ions found in living beings, Which are the main...

Which are the main positive ions found in living beings? The major cations found in living beings are the sodium cation (Na+), the potassium cation (K+), the calcium cation (Ca

Optic vesicle, Optic vesicle We have explained earlier that the presum...

Optic vesicle We have explained earlier that the presumptive material for the optic vesicles lies in qe eye field in the anterior region of the early neural plate. Experiments

What is the phenotype, What is the phenotype? Phenotype is every observ...

What is the phenotype? Phenotype is every observable characteristic of a living being conditioned by its genes. A few phenotypes may be altered by nongenetic factors (for insta

Pleural biopsy, Pleural Biopsy Pleural biopsy is one of the diagnostic...

Pleural Biopsy Pleural biopsy is one of the diagnostic procedure which may  cause lot of anxiety in  the patient. Procedure Pleural biopsies may be preformed surg

Explain oncogenes or defective tumour suppressor genes, 1.  Explain why a g...

1.  Explain why a gain of function would be a dominant effect and a loss of function would be a recessive effect. Which types of genes are each associated with: oncogenes or defect

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd