Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Stages of wilms tumour , Stages of Wilm's tumour  We shall now discuss...

Stages of Wilm's tumour  We shall now discuss about the various stages of Wilm's tumour. The extent of  disease is staged according to the findings of  surgery and  the presen

Coelentrata, Classification of coelentrata upto sub classes

Classification of coelentrata upto sub classes

How can the hypothesis that asserts that chloroplasts, How can the hypothes...

How can the hypothesis that asserts that chloroplasts as well as mitochondria were primitive prokaryotes that associated in mutualism with primitive anaerobic eukaryotic cells be c

Determine what the abnormal levels of afp signal, Determine what the abnorm...

Determine what the abnormal levels of AFP signal Abnormal levels of AFP may signal the following: Open neural tube Defect (ONTD) Down syndrome Other chromosoma1

Height of plants - quantitative characters, Height of Plants - Quantitative...

Height of Plants - Quantitative Characters The height of a plant is a very good indicator of their general performance and therefore, can be employed as a criterion of the suc

Explain casein, Explain Casein Casein is an important example of protei...

Explain Casein Casein is an important example of protein, which can be boiled without apparent change in stability. The exceptional stability of casein makes it possible to boi

Parthenogenesis, Parthenogenesis The diploid egg produced in the embr...

Parthenogenesis The diploid egg produced in the embryo sacs during diplospory and apospory develops into an embryo without fertilization, thus maintaining the sporophytic lev

Fluoride - mineral elements, FLUORIDE It is mostly available in drin...

FLUORIDE It is mostly available in drinking water. Fluoride is essential for the formation of enamel of the teeth. Deficiency of fluoride causes weakness of enamel.

Explain trapezoidal full mucoperiosteal flaps, Explain Trapezoidal Full Muc...

Explain Trapezoidal Full Mucoperiosteal Flaps a) Similar to rectangular flap except: The 2 vertical incisions are at obtuse angle with the Horizontal incision, b) Mostly

Ask, what are the names of karyotypes?

what are the names of karyotypes?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd