Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Lactation tetany of mares (eclampsia, Lactation tetany of mares (eclampsia,...

Lactation tetany of mares (eclampsia, transit tetany) Hypocalcaemia in mares causes lactation tetany, which is characterized by abnormal behaviour, in coordination and tetany.

Animal cloning, Animal Cloning: When entire animals are obtained from s...

Animal Cloning: When entire animals are obtained from somatic cells of an animal, it is known as animal cloning. Cloning is usual in plants but in case of animals only some deg

Diagnosis of multiple myeloma, Which of the following abnormal blood work r...

Which of the following abnormal blood work results is most closely associated with a diagnosis of multiple myeloma? A. Extremely high levels of abnormal lymphocytes B. Low potassiu

Cation saturation and nutrient absorption by plants, Cation Saturation and ...

Cation Saturation and Nutrient Absorption by Plants The availability of adsorbed cations is not always so easy as the above explanation might suggest. This is because, several

What are the male and the female gonads in humans, Q. What are gonads? What...

Q. What are gonads? What are the male and the female gonads in humans? Gonads are the organs that produce gametes they contain the germ cells that undergo division and generate

Characteristics of the digestive system of earthworm, Q Which are the organ...

Q Which are the organs and characteristics of the digestive system of earthworms related to the kind of diet of these animals? Small organisms ingested together with soil parti

Explain the alterations occurring in egg, Alterations occurring in egg ...

Alterations occurring in egg The quality, flavour, composition and functional properties of eggs are adversely affected more rapidly and to a greater extent by the speed and co

Explain rifampin, Options with Rifampin Standard 4-drug treatment regi...

Options with Rifampin Standard 4-drug treatment regimens including rifampin can be given to HIV-infected patients with active TB who are simultaneously receiving HAART if the H

Biotechnology, How does bisulfite sequencing work ?

How does bisulfite sequencing work ?

Reproduction and life cycles – protozoan, Reproduction and Life Cycles – Pr...

Reproduction and Life Cycles – Protozoan Asexual reproduction occurs in all protozoan through fission, budding and cyst formation. In this method the organism reproduces to fo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd