Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Medtronic-hall valve-types of valves, Medtronic-Hall Valve :  This is...

Medtronic-Hall Valve :  This is a low profile central tilting disc valve. The disc is made of pyrolitic carbon and housing is single piece of machined titanium. Introduced in

How hiv infection is mainly spread, It is mostly spread by sexual contact, ...

It is mostly spread by sexual contact, blood transfers and from HIV infected mother to child.

Define functions of inorganic phosphorus, Define Functions of Inorganic Pho...

Define Functions of Inorganic Phosphorus? The major functions of inorganic phosphorus include: a) Structural component of bones and teeth: Phosphorus is a part of calcium ph

Ideal eia and measures and benefits of eia, An ideal EIA system would: ...

An ideal EIA system would: (i)     Apply to all projects that are expected to have significant environmental effects and address all impacts that are expected to occur due to t

Symptoms of celiac disease, Q. Symptoms of celiac disease? The list of ...

Q. Symptoms of celiac disease? The list of symptoms as mentioned in various sources for celiac disease includes: • Digestive symptoms - diarrhoea, abdominal pain, abdominal

Salmonellosis, Salmonellosis The genus Salmonella comprises nearly 2500...

Salmonellosis The genus Salmonella comprises nearly 2500 serovars, traditionally based on Kauffman-White scheme in which H (flagelar) and O (somatic) antigens determine the ser

What is microtubule assembly, Microtubule assembly: A) generally originates...

Microtubule assembly: A) generally originates in the centrosome. B) Occurs only during mitosis. C) Occurs randomly throughout the cell. D) Is regulated by myosin. E) Is inhibited d

Determine the prosthetic planning, Determine the prosthetic planning Co...

Determine the prosthetic planning Complete prosthetic planning has to be done and the implants planned accordingly. Thereafter the surgical phase should be carried out. Once th

Identical chromatids or separation of homologous chromosomes, Q. During mit...

Q. During mitotic anaphase is there separation of identical chromatids or separation of homologous chromosomes? In the anaphase of mitosis the identical chromatids complete and

What do you mean by connective tissue proper, Q. What is connective tissue ...

Q. What is connective tissue proper? The name connective tissue proper is used to designate the connective tissue that fills interstitial spaces as opposed to the specialized c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd