Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Parasites - biological stress, Parasites - Biological Stress Many inse...

Parasites - Biological Stress Many insects and microorganisms can feed upon tissues and saps of living plants. Hence plants must be protected against such parasites either by

The klove roughness discrimination test, The Klove roughness Discrimination...

The Klove roughness Discrimination Test The subject must order four blocks covered with varying grades of sandpaper presented behind a blind with regards to degree of roughness

Explain esterification, Explain Esterification The process of convertin...

Explain Esterification The process of converting an acid into an alkyl or aryl derivative. Most frequently the process consists of the reaction of an acid with an alcohol in th

Maggots and murder, Plot the data as a suitable graph. Join the points with...

Plot the data as a suitable graph. Join the points with the best-fitting smooth curve.Temperature oC(5,10,11,13,17,19) Time taken for eggs to hatch/h 230 90 70 30 25

Phases of seed formation, Phases of Seed Formation The process of seed...

Phases of Seed Formation The process of seed formation can be divided into two phases: The division phase, in which fertilised egg divides mitotically and complet

Major divisions of the chordate phylum, Q. What are the two major divisions...

Q. What are the two major divisions of the chordate phylum? Chordates are divided into vertebrates and protochordates (urochordates and cephalochordates). Q. What are the d

Digestive system - liver, LIVE R (HEPAR) - Largest gland of body. W...

LIVE R (HEPAR) - Largest gland of body. Weighing 1.6 kg. Form 1/40 of the body weight. Bio-chemical laboratory. Bussiest part in whole river of life. Dark reddish brown

Stress - responses of plants to stress, Stress - Responses of Plants to Str...

Stress - Responses of Plants to Stress Let us recall what happens within the natural communities occupying the same habitat. The relative location of two plants may place them

What is coevolution, What is Coevolution ? There is considerable eviden...

What is Coevolution ? There is considerable evidence that supports an interesting theory that two individual species can affect each others evolution in reciprocal fashion. In

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd