Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Define r and k strategists, Define r and K Strategists? Ecologists have...

Define r and K Strategists? Ecologists have identified two major types of reproductive strategies among species. Characteristic patterns can be seen among species relating the

Explain brachial or radial approach, Q. Explain Brachial or Radial Approach...

Q. Explain Brachial or Radial Approach ? This technique involves performing the coronary angiogram through the right brachial artery in the right ante-cubital fossa. Usually a

Discuss about the function of tear film in short, Discuss about the functio...

Discuss about the function of Tear Film in short. Functions of Tear Film The normal function of the eye is dependent on sufficient supply of tear fluid- a secretion

Explain the factors affecting gi of foods, Explain the Factors Affecting GI...

Explain the Factors Affecting GI of Foods? A variety of factors affect GI of foods. The factors which affect the rate of glucose absorption from starchy foods and therefore the

How many cc of basaljel in the medicine cup, The doctor ordered 3 teaspoons...

The doctor ordered 3 teaspoons of Basaljel suspension in water or juice. How many cc of Basaljel (aluminum carbonate) should the nurse place in the medicine cup?

In high altitudes is it necessary for the blood to have more, In high altit...

In high altitudes is it necessary for the blood to have more or less hemoglobin? In high altitudes the air is rarefied and oxygen concentration is lower than in low altitudes.

steps of c-dna creation and mrna, C dna is made from a mature m rna of euk...

C dna is made from a mature m rna of eukaryotic cell with the utilization of enzyme called as reverse transcriptase. Steps in c dna creation 1. m rna is made and purified f

Examples of natural selection, EXAMPLES OF NATURAL SELECTION - 1 .   ...

EXAMPLES OF NATURAL SELECTION - 1 .       INDUSTRIAL MELANISM - First studied by Fischer & Ford (1845) on pepperd moth (Biston betularia) of two types : grey moth (B.

Development of pollen in cyperaceae, Development of Pollen in Cyperaceae ...

Development of Pollen in Cyperaceae In all members of the Cyperaceae out of the four nuclei formed after meiosis, only one functions, the others degenerate. The functional nuc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd