Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Cell get oxaloacetic acid for krebs citric acid cycle, From where does the ...

From where does the cell get oxaloacetic acid for krebs citric acid cycle? Oxaloacetic acid is also formed when pyruvate is acted upon by pyruvate carboxylase in response to an

Define immediate preoperative period during surgery, Define Immediate Preop...

Define Immediate Preoperative Period during Surgery? Nothing should be given by mouth for at least 8 hours before a general surgery so that the stomach has no leftover food at

Define endocytosis of glut4 transporters, Define endocytosis of GLUT4 Trans...

Define endocytosis of GLUT4 Transporters Person X is a healthy human who has volunteered to take experimental drug Y.  Person X has a normal dinner at 6 PM on April 1 and then

What is the climax stage of an ecological succession, Q. What is the climax...

Q. What is the climax stage of an ecological succession? The climax stage is the stage of an ecological succession in which the community of an ecosystem turn into stable and d

Arteries - circulation, Arteries - Circulation The arteries deliver bl...

Arteries - Circulation The arteries deliver blood from the heart. Figure shows the structure of arteries and the different layers of the vessel wall. The thick walls of these

Applications in horticulture and agriculture, Applications in Horticulture ...

Applications in Horticulture and Agriculture While going round in public parks, you might have observed plants trimmed in such a way giving appearances of animals like camel,

Theory of embryology - pre-formation theory, PR E FORMATION THEORY - ...

PR E FORMATION THEORY - Proposed by Marcello Malpighi. Studied chick egg and proposed his work in "De ova-incubato". According this different part of embryo previousl

What are the use of a barrier membrane is grafting procedure, What are the ...

What are the uses of a barrier membrane is grafting procedures? Uses of barrier membrane in grafting procedures : i) Help in stabilizing the graft ii) Prevents ingrowth

Prevent and control staphylococcal food poisoning, To prevent and control S...

To prevent and control Staphylococcal food poisoning: • Avoid contamination of foods • Kill organism by heating, refrigeration • Personnel hygiene • Adequate cookin

Define the food processing, Define the Food Processing? We are all awar...

Define the Food Processing? We are all aware that delay in the use of fresh foods, alters its freshness, palatability and nutritive value. Therefore, it becomes very important

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd