Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Define the term- adaptation, Define the term- Adaptation The terms sco...

Define the term- Adaptation The terms scotopic and photopic vision are relative to the lighting conditions of an individual place. Light adaptation is a very quick process and

Major events during gastrulation, MAJOR EVENTS DURING GASTRULATION - 1....

MAJOR EVENTS DURING GASTRULATION - 1.       There is rearrangement of cells due to morphogenetic movement of the cells of blastoderm. 2.       The three primary germ layer g

Preparation of tlc plate, i want to extract my compound of interest from tl...

i want to extract my compound of interest from tlc i m finding some enzyme inhbitor from microbial source can you plzz suggest me how to prepare nad get my compound

Explain radiation sterilization, Explain radiation sterilization Variou...

Explain radiation sterilization Various studies have been conducted which show the effect of processing on vitamins especially, thiamine. In one study, which compared the effec

Describe the phylum arthropoda and metamorphosis, Describe the Phylum Arthr...

Describe the Phylum Arthropoda and Metamorphosis? More than one million different species of arthropods are estimated to exist on the earth today-more than all other animal spe

Dot blot, Dot blot  is the technique for measuring the amount of one pertic...

Dot blot  is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitroc

How to reduce the stresses on the implant, How to reduce the stresses on th...

How to reduce the stresses on the implant i) There is need to reduce the stresses on the implant - ii) Frequent recall for maintenance or hygiene appointments. iii) Gingi

Fragmentation - types of asexual reproduction, Fragmentation - Types of Ase...

Fragmentation - Types of Asexual Reproduction Fragmentation is a phenomenon where parent animal spontaneously (on its own accord) splits into two or more fragments. Each one o

What are the main water properties, What are the main water properties that...

What are the main water properties that make water special for life? The water properties that make water biologically significant are molecular polarity, thermal stability (el

Find human anatomy & physiology laboratory manual, I can not find Human Ana...

I can not find Human Anatomy & Physiology Laboratory manual 10th edition cat version by Marieb/mitchell in expertsmind homework help, I have the text book just need to make sure I

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd