Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Briefly describe about the micro minerals, Briefly Describe about the Micro...

Briefly Describe about the Micro Minerals? The last unit focused on the macro minerals. Now in this unit we will study about the micro minerals, namely, iron, zinc, copper, sel

Values of dpd for plant cells under hypertonic media, Q. What are the value...

Q. What are the values of DPD for plant cells under hypertonic, hypotonic and isotonic media? In plant cells under hypertonic medium there is loss of water for the external, SF

Endomitosis, #questwhy and where endomitosis happens?ion..

#questwhy and where endomitosis happens?ion..

Zoo flagellates, #question names of 10 zoo flagellates

#question names of 10 zoo flagellates

FISHERIES, describe the endoskeleton of fish?

describe the endoskeleton of fish?

Gases will be taken in and given out by a green plant, What gases will be t...

What gases will be taken in and given out by a green plant (a) in darkness, (b) in bright sunlight?   a) In low light intensities a green plant will be taking in oxygen and

Circulatory system, Circulatio n I n  Animal Circulation is served ...

Circulatio n I n  Animal Circulation is served by a fluid as blood/ lymph that circulates through the body in a regular manner, receiving & distributing materials in the wa

How vitamin e provide synthesis of enzymes and proteins, How Vitamin E prov...

How Vitamin E provide Synthesis of enzymes and proteins? 1) Synthesis of enzymes and proteins: It serves as a co-repressor in the synthesis of certain enzymes and plays a speci

Define gray matter and white matter of the spinal cord, Q. What are the res...

Q. What are the respective constituents of the gray matter and of the white matter of the spinal cord? The gray matter or gray substance of the spinal cord contains predominant

Goal of dietary management for gdm, Q. Goal of dietary management for GDM? ...

Q. Goal of dietary management for GDM? The goal of dietary management for GDM is to provide the pregnant woman with adequate energy, commonly known as "calories", and to ensure

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd