Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Plants - slow moving waters, Plants - Slow Moving Waters Plant life is...

Plants - Slow Moving Waters Plant life is abundant in this habitat and includes rooted vascular plants such as pond weeds and grasses, firmly attached aquatic mosses and multi

Major organic molecules for living beings, Q. What are the major purposes o...

Q. What are the major purposes of the organic molecules for living beings? Organic molecules, like, lipids, carbohydrates and proteins perform several functions for living orga

Nutrition, The oxidation of sugar in the cell of higher organisms takes pla...

The oxidation of sugar in the cell of higher organisms takes place in the ?

Photophosphorylation, explain the role of cyclic photophosphorylation in p...

explain the role of cyclic photophosphorylation in photosynthesis

Define food sources of calcium, Define Food Sources of Calcium? Dairy p...

Define Food Sources of Calcium? Dairy products are of course the primary source of calcium followed by grains and pulses. Among the millets, ragi contains substantial amount of

Meiosis, Why is it necessary to have the second division in meiosis even th...

Why is it necessary to have the second division in meiosis even though cells have already been reduced to haploid number after the first division?

Control of glycogen metabolism, The major factor which controls glycogen me...

The major factor which controls glycogen metabolism in the liver is the concentration of phorphorylase alpha. Certainly, this enzyme catalyzes the limiting step of glycogen breakdo

Explain pasteurization - method of food preservation, Explain Pasteurizatio...

Explain Pasteurization (temperature below 100° C) - method of food preservation? Pasteurization is a heat treatment that kills a part but not all the microorganisms present and

Define about the lactose, Define about the Lactose? Lactose has a molec...

Define about the Lactose? Lactose has a molecular structure consisting of galactose and glucose. It is of interest because it is associated with lactose intolerance which is th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd