Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Name an artificial fertiliser which farmers can use, Name an artificial fer...

Name an artificial fertiliser or fertilisers which farmers can use to increase the supply of nitrate, phosphate and potassium to their crops.   NPK compound fertiliser havin

Explain transposition the great arteries with ventricular, Explain Transpos...

Explain Transposition the Great Arteries with Ventricular Scptal Defect ? Transposition of the great arteries with VSD is an indication for surgery. It is preferable to do VSD

Explain the relation between chromatin and chromosome, What is the relation...

What is the relation between the concepts of chromatin and chromosome? Are euchromatin and heterochromatin part of chromosomes? Every filament of chromatin is a complete DNA mo

Discovery of plant hormones, Discovery of Plant Hormones To date, five...

Discovery of Plant Hormones To date, five major classes of plant hormones have been discovered namely auxins, gibberellins, cytokinins, abscisic acid and ethylene. It is possi

Zoology, why phylum protozoa is called protozoa?

why phylum protozoa is called protozoa?

Hemocytometer, A device called a hemocytometer is often used to estimate th...

A device called a hemocytometer is often used to estimate the number of cells in a culture. Cells are suspended in fluid. A small volume of the fluid is placed into a special chamb

Zoloogy, two main evolutionary novelties presented by annelids

two main evolutionary novelties presented by annelids

Show the energy pyramids, Q. Can the amount of available energy in a given ...

Q. Can the amount of available energy in a given trophic level be larger than the available energy in inferior trophic levels? What does that condition means to the conformation of

Show foods that damage gi mucosa, Q. Show Foods that damage GI mucosa? ...

Q. Show Foods that damage GI mucosa? Foods that damage GI mucosa: A number of spices, herbs and other condiments have been found to have little or no irritating effect on the m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd