Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Explain the terms - sulphur and boron, Sulphur and Boron Boron is extra...

Sulphur and Boron Boron is extracted with hot water. Sulphur is extracted with 500 ppm solution of P solution (prepared from potassium dihydrogen orthophosphate or monocalcium

Explain the nutritional and functional role of zinc, Minerals  :-  Zinc ...

Minerals  :-  Zinc Food Source      Meats, cereals.  Nutritional Functional role Essential nutrient: Deficiency produces loss of appetite, growth retardation, skin

Explain galactosemia, Galactosemia Inability  of conversion of galactos...

Galactosemia Inability  of conversion of galactose  to glucose results in the accumulation of galactose in  the  blood  -  known  as galactosemia. The biochemical defect usuall

Prevention of overloading the sterilizer, Prevention of overloading the ste...

Prevention of overloading the sterilizer Most sterilizers have a system to notify the operator of mechanical malfunction, but sterilizers cannot notify the operator whether the

Explain the objectives of nutritional care, Explain the Objectives of nutri...

Explain the Objectives of nutritional care? - To minimize the development of nutrient imbalance. - To maintain fluid and electrolyte homeostasis - To promote energy equil

What is linkage, What is linkage? Two genes are assumed to be under lin...

What is linkage? Two genes are assumed to be under linkage, or linked, when they reside in the same chromosome. For instance, the research of the human genome discovered tha

Pathophysiology of mitral stenosis, Q. Pathophysiology of mitral stenosis? ...

Q. Pathophysiology of mitral stenosis? Normally there is no pressure gradient between left atrium and the left ventricle during diastole. However, as the valve orifice size dec

What is the critical photoperiod, What is the critical photoperiod? How can...

What is the critical photoperiod? How can the critical photoperiod relate to flowering be experimentally determined? The critical photoperiod is the limit of the photoperiod du

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd