Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Explain about the family and social history - assessment, Explain about the...

Explain about the Family and Social History - Assessment  Recent studies suggest that genetic variation plays an important role in etiology of learning problems. Hence, the col

Explain how movement is controlled in protozoans., Movement is even control...

Movement is even controlled by organelles, cilia and flagella. These and the pseudopodia in the amoebas give these small creatures movement, something we associate with animals. Es

Transport into the cytosol, Fatty  acids  are  synthesized  in  the  cytoso...

Fatty  acids  are  synthesized  in  the  cytosol,  but  acetyl  CoA  is  produce  from pyruvate in the mitochondria. Therefore the acetyl CoA must be transferred  from  the mitocho

Explain difference between an ecological niche and a habitat, What is the d...

What is the difference between an ecological niche and a habitat? An ecological place is a set of peculiar activities, resources and strategies that a species explores to repro

Explain the types of fats, Jillian and Michael are in their freshman year a...

Jillian and Michael are in their freshman year at college. The two have been friends since grade school and the two of them enjoy getting together for dinner. Michael typically

Eczema and dermatitis, Eczema and Dermatitis: Eczema and Dermatitis ar...

Eczema and Dermatitis: Eczema and Dermatitis are a common problem  all over the world. Their incidence is 2-3  per cent of all medical problems seen  in practice (about 30 per

Brain, What are the types of brain?

What are the types of brain?

Show the anatomical evidence, Q. Show the Anatomical Evidence? Anatomy ...

Q. Show the Anatomical Evidence? Anatomy is the study of the structure, organisation and development of cells and tissues of plants and animals. For over a century taxonomists

Genetics, what about cytoplasmic sex determination

what about cytoplasmic sex determination

Polarity in regeneration, Polarity in Regeneration A distinct polarit...

Polarity in Regeneration A distinct polarity or gradient exists in planarians (and in other animals). The meaning of this is that in any piece or segment of the body; its ant

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd