Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Epilimnion - summer stratification, Epilimnion - Summer Stratification ...

Epilimnion - Summer Stratification This forms the upper layer of the lake and consists of freely- circulating warm water which is well lighted though poor in nutrients. Most o

Pathogenesis of viruses, Pathogenesis of Viruses Viral diseases An...

Pathogenesis of Viruses Viral diseases An indicated before, viruses cause a number of infectious human diseases from minor ailments like common cold, to fatal disease, lik

Guanotelism - excretion, Guanotelism - Excretion Normal ...

Guanotelism - Excretion Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

How the molecule would increase in mass slightly, Suppose many of the atoms...

Suppose many of the atoms of nitrogen in a double-stranded molecule of DNA, of isotope N-14, were replaced with the isotope N-15. What afffect would this have? a) The nitrogeneo

What are lateral meristems, What are lateral meristems? Where can they be f...

What are lateral meristems? Where can they be found and which type of plant growth do they promote? Lateral, or secondary, meristems, are the cambium and the phellogen (also ca

Analyze the chromosomes of her daughters or of her sons, Is it more indicat...

Is it more indicated for a geneticist desiring to map the X chromosome of the mother of a given family (the researcher does not have access to her DNA, only access to the genetic m

What is pus, What is pus Pus is a residual of the inflammatory reaction...

What is pus Pus is a residual of the inflammatory reaction. It is having a mixture of fragments of dead leukocytes, infectious agents (generally bacteria) and tissues.

Define hexose monophosphate pathway, Define hexose monophosphate pathway ...

Define hexose monophosphate pathway The hexose monophosphate pathway (HMP also called the pentose phosphate pathway, or phoshogluconate pathway) consists of Mo irreversible o

Explain the toxicity of vitamin d, Explain the Toxicity of Vitamin D? ...

Explain the Toxicity of Vitamin D? The adverse effects of high vitamin D intakes include hypercalciuria (excessive urinary calcium excretion) and hypercalcaemia (high concentr

Show the major proteins that constitute the sarcomere, Q. What are the majo...

Q. What are the major proteins that constitute the sarcomere? What is the function of those molecules in the muscle cells? In the sarcomere there are organized myosin and actin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd