Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Explain different photic energy frequencies, What is an example of a lab ex...

What is an example of a lab experiment that shows the variation of the photosynthesis efficiency in relation to different photic energy frequencies to which the reaction is exposed

Pyruvate carboxylase activation, Oxaloacetate has two main roles. It is an ...

Oxaloacetate has two main roles. It is an intermediate which is consumed in gluconeogenesis and it is also a key intermediate in the citric acid cycle where it fuses with acetyl Co

What are the hormones secreted by the adrenal medulla, What are the hormone...

What are the hormones secreted by the adrenal medulla? What are their respective functions? The medullary portion of the adrenals secretes hormones of the catecholamine group:

Biogeochemical cycle, what are the effects of mining and farming on the pho...

what are the effects of mining and farming on the phosphorus cycle?

Explain beginning and the end of the third interphase period, Q. What are t...

Q. What are the events that mark the beginning and the end of the third interphase period? What happens in the cell in this period? The third interphase period is the G2, It be

Microbiology, what are the two alternate pathways to glycolysis

what are the two alternate pathways to glycolysis

Symptoms and complications of atherosclerosis, Q. Symptoms and Complication...

Q. Symptoms and Complications of atherosclerosis? Symptoms Excessive weight, hypertension, high levels of cholesterol and triglycerides. Complications Myocardia

Can you explain nerves, Q. What are nerves? Axons extend all through th...

Q. What are nerves? Axons extend all through the body inside nerves. Nerves are axon-containing structures presenting many axons and covered by connective tissue. The nerves co

Define interactions between volatile substances and proteins, Define Intera...

Define Interactions between Volatile Substances and Proteins? Flavour binding may involve adsorption at the surface of food or penetration to the food interiority by diffusion

Determine the term - test-retest reliabilities, Determine the term - Test-r...

Determine the term - Test-retest reliabilities The test manual reports reliability data. Test-retest reliabilities for the 13 main scales range from .78 to .96. The problem of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd