Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

What is community ecology explain their characterstics, What is Community E...

What is Community Ecology explain their characterstics? Community Ecology: In ecological terms, a community consists of an assemblage of all of the populations living and int

Agro industrial-reproductive tract infections, Reproductive tract infection...

Reproductive tract infections The major cause of reproductive tract infections are, improper dry cow nutrition, contamination at calving due to unsanitary calving area; virule

Investigation of tricuspid stenosis by echocardiography, Q. Investigation o...

Q. Investigation of Tricuspid stenosis by Echocardiography? Findings are subtle. One should carefully search for organic tricuspid valve involvement in all rheumatic heart dise

Explain the working of conical flasks, Explain the working of Conical Flask...

Explain the working of Conical Flasks? Conical flasks of different volumes can be used for media preparation and also for culturing large amount of microorganisms in liquid med

Nucellus - seed, Nucellus - Seed In a large majority of flowering plan...

Nucellus - Seed In a large majority of flowering plants the nucellus is gradually utilized by the endosperm or embryo. In leguminous seeds, for example, the nucellus degenerat

What is the general chemical equation of photosynthesis, What is the genera...

What is the general chemical equation of photosynthesis? Why doesn't that equation clearly show the real origin of the molecular oxygen liberated? The general equation of photo

Explain amantadine, Amantadine Treatment with oral amantadine or rimant...

Amantadine Treatment with oral amantadine or rimantadine begun within 48 hours after the onset of illness decreases the duration of fever and symptoms by about 1 day. Whether t

What are sori, What are sori? The word "sori" is the plural form of "so...

What are sori? The word "sori" is the plural form of "sorus". In ferns, a sorus (pl. sori) is a cluster of sporangia on the edge or underside of a fertile frond. In lots of spe

Explain the failure of endodontic Treatment, Explain the Failure of Endodon...

Explain the Failure of Endodontic Treatment 1. Microorganisms are left inside the canal - Improper/inability of cleaning and shaping, - Source of infection could be: peri

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd