Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Describe two differences among green algae and red algae, Some biologists p...

Some biologists prefer to categorize brown, red, and some green algae as plants rather than protists. What characteristics of these algae support like classification? Brown and

Reducing pollution - conservation of wildlife, Reducing Pollution - Conserv...

Reducing Pollution - Conservation of wildlife As you already know, pollution of various kinds has affected the survival of living beings, particularly the wildlife in various

Phase (different interference) contrast microscopy, Phase (different interf...

Phase (different interference) Contrast microscopy: Living cells are mostly   transparent. For viewing under ordinary light microscope, therefore, live cells must be stained wi

Define feeding options for premature and lbw babies, Define Feeding Options...

Define Feeding Options for Premature and LBW Babies? Different workers have tried different method and since all methods are successful, it is dependent on the individual infan

Nursing process in infective endocarditis, Nursing Process Nursing Ass...

Nursing Process Nursing Assessment Health history-Past-valvular, congenital or syphilitic cardiac disease/cardiac surgery. Habits-IV drug abuse. Assess the signs an

Explain fluids, Explain Fluids Fluids: Fluid diets are given to patien...

Explain Fluids Fluids: Fluid diets are given to patients with more advanced dysphasia or fractured jaws. The diet may include fruit juices, thin strained porridge with milk, e

Define insect resistant crops in ecological way, Insect resistant crops (IR...

Insect resistant crops (IRC) Should reduce use of insecticides. This is beneficial to environment because:  -  More beneficial insects around as they are not killed by in

Different types of gas exchange in animals, Q What are the different kinds ...

Q What are the different kinds of gas exchange that occur in animals? In beings from the kingdom Animalia the gas exchange may occur either by diffusion, cutaneous respiration,

Define other consideration for dietary management, Define other considerati...

Define other consideration for Nutrient Requirement and Dietary Management Oral intake is generally feasible only during the anabolic phase wherein the patient should be given

Define the term- life on earth, The article entitled "Life on Earth" is try...

The article entitled "Life on Earth" is trying to make the point that RNA likely included before DNA. Forthis article which of the following is false? A. Chains of RNA nucleoti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd