Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Causes of noise pollution, 1.      Road traffic:  of all the sources of noi...

1.      Road traffic:  of all the sources of noise pollution, road traffic is the most prevalent and perhaps the most damaging source of pollution. More people are exposed to noise

Define plate counts and colony forming unit, Define Plate Counts and Colony...

Define Plate Counts and Colony Forming Unit? Plate counts is the most frequently used method to measure microbial numbers. The technique, however, can be used only for those mi

What are the main cells of which poriferans are made, What are the main cel...

What are the main cells of which poriferans are made? Sponges have their outer wall covered by flat cells known as pinacocytes and having pores well-delimited by special cells

Show the ph range of natural honey, Q. Show the pH range of natural honey? ...

Q. Show the pH range of natural honey? The pH of natural honey ranges from 3.4 to 6.1. Acidity of honey is primarily due to presence of acids such as gluconic acid, pyruvic a

Why is the fish circulation classified as simple circulation, Why is the fi...

Why is the fish circulation classified as a simple and complete circulation? Complete circulation is that in which there is no mixture of venous blood and arterial blood. As ci

What are the main negative ions found in living beings, What are the main n...

What are the main negative ions found in living beings? The major anions found in living beings are the chlorine anion (Cl-), the phosphate anion (PO4--), the bicarbonate anion

Determine about the accommodation of eye, Determine about the Accommodation...

Determine about the Accommodation of eye Accommodation is the ability of the overall refracting power of the eye to change to clearly view objects at different distances. This

On average what is the life duration of the red blood cells, On average wha...

On average what is the life duration of the red blood cells? Where are they destroyed? What is the destination of the heme groups after the destruction of hemoglobin molecules?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd