Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Chest and leg wound complications, Chest and Leg Wound Complications ...

Chest and Leg Wound Complications :  The patients under going CABG are usually elderly, obese and nearly a quater of them are diabetic. So sonic of them get superficial or d

Amoeba, AMOEBA Systematic  Position Amoeba  was discovered by  Rus...

AMOEBA Systematic  Position Amoeba  was discovered by  Russel von Rosenhoff in  1755. He  called it the little proteus  after the mythical  sea god who is believed to be c

Wet stigma - category of stigma, Wet Stigma - Category of Stigma Petun...

Wet Stigma - Category of Stigma Petunia shows several randomly distributed 2-celled papillae on its surface. In a developing stigma, the epidermis is covered by a continuous,

Photolithoautotrophs - nutritional types of microorganisms, Explain Photoli...

Explain Photolithoautotrophs - Nutritional Types of Microorganisms? These microorganisms use light as an energy source, inorganic substance as electron source and CO 2 as the

Why do sharks need to agitate their body to swim, How dissimilar are the sw...

How dissimilar are the swimming strategies in osteichthyes and in chondrichthyes? Why do sharks need to agitate their body to swim while bony fishes do not? Bony fishes have a

Chorea, It is found in around 20 per cent cases of ARF and it is a late man...

It is found in around 20 per cent cases of ARF and it is a late manifestation occurring even 3 months after GAS pharyngeal infection. Chorea is triggered by emotional disturbances

Explain about the freeze drying Method, Explain about the freeze drying Met...

Explain about the freeze drying Method? The Techniques of freeze-drying have been developed over the past half-century for the purpose of preserving certain biological material

What is the heterophagic intracellular digestion, Q. What is the heterophag...

Q. What is the heterophagic intracellular digestion and How is this process accomplished? Heterophagic intracellular digestion is the breaking into smaller substances of extern

Molecules that make possible active transport, Which are the molecules that...

Which are the molecules that make possible active transport through membranes? Ans) Active transport is made by particular membrane proteins. These proteins are known as "pumps"

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd