Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Haemo filtration-open-heart surgery, Haemo Filtration : Ultra filtration d...

Haemo Filtration : Ultra filtration during open heart surgery helps in removing excess fluid, especially in renal failure patients. Patients are haemo diluted (haematocrit 18-20)

Limitation of eia, EIA suffers from following limitations: (i)     ELA s...

EIA suffers from following limitations: (i)     ELA should be undertaken at the policy and planning level rather than at the project level. (ii)   Range of possible alternati

Which part of the cell have an excess of negative charge, Given how the Na+...

Given how the Na+/K+ pump works, which part of the cell (inside or outside) will have an excess of negative charge?

Explain the cori cycle, The  Cori cycle a)  Pyruvate formed from glucos...

The  Cori cycle a)  Pyruvate formed from glucose is converted  to lactate by lactate dehydrogenase in the muscle cell. b)  Lactate is released into  the blood and taken up b

Determine the role as antioxidant, Determine the Role as antioxidant? S...

Determine the Role as antioxidant? Some carotenoids, in addition to serving as a source of vitamin A, have been shown lo function as antioxidants. Studies show that lycopene. (

Our healthi diet, in what form is most carbohydrates is taken in normal die...

in what form is most carbohydrates is taken in normal diet

What are the lateral lines of fishes, What are the lateral lines of fishes?...

What are the lateral lines of fishes? The lateral lines of bony fishes are sense organs that extend along both sides of the animal body. They make contact with the environment

Chemical stress - nature of the solutes, Chemical Stress - Nature of the so...

Chemical Stress - Nature of the solutes The acidic or basic reaction of soil and water of a particular habitat reflects its geochemical history beginning with its formation an

Define the patient care related to neurological disorder, Define the patien...

Define the patient care related to neurological disorder? A few additional points related to the patient care that need to be kept in mind include paying attention to the patie

Define the indications for root-end resection apicoectomy, Define the Indic...

Define the Indications for Root-End Resection Apicoectomy a) Persistent..... "Exactly the SAME of Curettage except the biopsy. b) When the apical portion of the root canal s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd