Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Difference between spermatocyte i and spermatogonium, Q. What is the differ...

Q. What is the difference between spermatocyte I and spermatogonium? The male germ cells are the spermatogonia (diploid cells, 2n) situated in the testicles and they mature an

How emulsion are formed, How emulsion are formed The most common method...

How emulsion are formed The most common method of preparing an emulsion is by mechanically dispersing one liquid phase in another by formation of small droplets. This takes pla

How does plasma membrane regulate, How does a plasma membrane regulate move...

How does a plasma membrane regulate movement of molecules into and out of a cell?

Briefly explain about type specimens, Q. Briefly explain about Type Specime...

Q. Briefly explain about Type Specimens ? The specimens on which the names of the species are based are kept as type specimens. Do not replace these specimens because these wi

Physical and chemical factors that affect the pasteurization, Define the Ph...

Define the Physical and Chemical Factors That Affect the Pasteurization? There are a few other physical and chemical factors that affect the pasteurization method. These involv

Complete test of hypothesis, Conduct a test of hypothesis to verify whether...

Conduct a test of hypothesis to verify whether a difference exists among the average numbers of ant species found at sites in the two regions of Mongolia. Use  .  You will require

Name the one autosomal dominant and autosomal, Name the one autosomal domin...

Name the one autosomal dominant and autosomal recessive Mendel an disorder in Humans. a) How is the action of exonucluease dissimilar from that of end nuclease? b) India has

What is the importance of vitamin B6, What is the Importance of vitamin B 6...

What is the Importance of vitamin B 6 In the human and animal organisms, vitamin B 6 acts in the form of pyridoxal. In the form of 5'-orthophosphate (Pyridoxal-5'-phosphate),

Agro industrial-mineral biofortification of plants, Mineral biofortificatio...

Mineral biofortification of plants One sustainable agricultural approach to reducing the mineral deficiencies in livestock animals is to enrich major staple food crops (rice,

What is meant by the peripheral nervous system, Q. What is meant by the per...

Q. What is meant by the peripheral nervous system (PNS)? The peripheral nervous system comprehends the ganglia and nerves of the body.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd