Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Changes in chromosome number, Aneuploidy : One or inore chron~osomes of a n...

Aneuploidy : One or inore chron~osomes of a normal set nlay bc lacking or present in excess. The term riullison~y refers to absence of both the chromosomes of a pair. Monosomy, tri

Determine the enzymes utilization in food industry, Enzymes utilization in ...

Enzymes utilization in food industry  Enzymes may be used in industry as components of living cells or after isolation in free or immobilized forms. All of them may be referred

Define prevention in the prevalence of overweight or obesity, Define Preven...

Define Prevention in the prevalence of overweight or obesity? It is believed that the increase in prevalence of obesity worldwide is more due to the environment that has become

Function of noradrenaline in consciousness, Q. Function of Noradrenaline in...

Q. Function of Noradrenaline in consciousness? Increased level of noradrenalin is implicated in wakefulness. Locus coeruleus noradrenergic neurons decrease their rate of firing

Evolutionary species concept, The evolutionary species concept which ident...

The evolutionary species concept which identifies a species as a "lineage (i.e. ancestor descendant sequence of population) evolving separately from others with its own unitary ro

Plants and animals - biological weathering, Plants and animals The acti...

Plants and animals The activity of living organisms like lichens, fungi, bacteria, blue green algae, bryophytes is also responsible for weathering.  It is referred to as biolog

Plant physiology, application of apical dominace in horticulture and agricu...

application of apical dominace in horticulture and agriculture short notes

How is carbon dioxide released by cellular respiration, How is carbon dioxi...

How is carbon dioxide released by cellular respiration transported from the tissues to be eliminated through the lungs? In vertebrates almost 70% of the carbon dioxide is trans

Determine the purposes of vital signs, Determine the purposes of vital sign...

Determine the purposes of vital signs The vital signs reflect the functioning of vital organs of the body i.e. heart, brain and lungs. They indicate the basic functions of body

Why healthy skeletal muscle fiber is isolated, Why healthy skeletal muscle ...

Why healthy skeletal muscle fiber is isolated A healthy skeletal muscle fiber is isolated and has no external forces on it.  It has normal intracellular levels of ATP and is ba

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd