Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Discuss chemical examination of semen, Question 1 Discuss briefly the c...

Question 1 Discuss briefly the collection and examination of pericardial fluid Question 2 Discuss the following- A) Collection of CSF B) Physical examination o

Preference share capital or quasi-equity, Preference Share Capital or Quasi...

Preference Share Capital or Quasi-Equity It is also called quasi-equity because it combines features of equity and those of debt.  It is preference because it is preferred to

Genes and alleles, Genes and Alleles The inheritance of any character c...

Genes and Alleles The inheritance of any character can be studied only when thcre are two contrasting conditions, such as yellow versus green seed colour (as observed by Mendel

Why is diabetes known as impairment to the homeostasis, Describe normal reg...

Describe normal regulation of blood glucose via the pancreatic hormones. How is this altered with Diabetes? Why is Diabetes called an impairment to the homeostasis of glucose? What

Explain the process of absorption of fats, Explain the process of Absorptio...

Explain the process of Absorption of Fats? After digestion, only 25% of triglycerides are broken completely to glycerol and fatty acids. Major digestion product is 2-monoacylgl

What are the constituent elements of the blood, Q What are the constituent ...

Q What are the constituent elements of the blood? The blood is made of a liquid and a cellular part the fluid part is called as plasma and in it there are several substances li

What are the moderate intensity exercises, Q. What are the moderate intensi...

Q. What are the moderate intensity exercises? - Walking for 1/2% hr or jogging - Biking leisurely for 1/2 hr. - Playing tennis, swimming, gardening, golfing - Vacuumin

Is there a larval stage in echinoderms, Q. Is there a larval stage in echin...

Q. Is there a larval stage in echinoderms? In echinoderms embryonic development is indirect, with ciliated larvae.

Explain why statement is logical in terms of kin selection, Biology once sa...

Biology once said why this statement is logical in terms of kin selection and use the coefficient of relatedness he'd be willing to lay down his life to save two brothers or eight

What is the difference between anabolism and catabolism, What is the differ...

What is the difference between anabolism and catabolism? Metabolism comprises two opposing processes: anabolism and catabolism. Anabolism is a set of synthesis reactions that t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd