Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Metamorphosis, What type of metamorphosis do spongs go through is it reteog...

What type of metamorphosis do spongs go through is it reteogressive?

How does a dna vaccine prevent future disease, How does a DNA vaccine preve...

How does a DNA vaccine prevent future disease? A DNA vaccine having DNA from a pathogen but cannot cause disease. When the vaccine is injected into a patient, the DNA directs t

Role of citric acid cycle, The citric acid cycle, also called as the tricar...

The citric acid cycle, also called as the tricarboxylic acid (TCA) cycle or Krebs cycle (after its discoverer in the year of 1937), is used to oxidize the pyruvate making during th

What is the role of pharmacologic therapy, What is the role of pharmacologi...

What is the role of pharmacologic therapy in the treatment of peri implantitis. Pharmacological therapy for patients presenting with an ailing implant involves subgingival irri

How to reduce calorie intake from fat, How to reduce calorie intake from fa...

How to reduce calorie intake from fat? Efforts should be made to increase the nutrition knowledge of the general public through mass media. The foods with lower fat co

Etiological factor of diverticular disease, Q. Etiological factor of divert...

Q. Etiological factor of diverticular disease? The causes of diverticular disease are not certain, but several factors may contribute to changes in the wall of the colon. These

Define the symptoms in bone marrow of pernicious anaemia, Define the sympto...

Define the symptoms in Bone marrow of pernicious anaemia? The nucleated red cells of the marrow are greatly increased.  The successive nucleated cell stages in erythropoiesis a

Define water losses by visible perspiration, Define water losses by visible...

Define water losses by visible perspiration? The water losses by visible perspiration are highly variable; the amount could be as high as 4L in hot climate or during strenuous

Explain jugular venous pressure and pulsation in details, Explain Jugular...

Explain Jugular venous pressure and pulsation in details? The jugular venous system is in direct continuity to right atrium in systolic extending to right ventricle in diast

Genomic dna fragments, You have an extensive library of genomic DNA fragmen...

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd