Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Types of stem cells-pulp tissue revascularization, Embryonic stem cells: c...

Embryonic stem cells: can differentiate into all cell types of the specialized embryonic tissues when given sufficient and necessary stimulation for a specific cell type, e.g. Plu

Role of microorganism in fermentation foods, Normal 0 false f...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Mitochondria be considered the power plants of the cell, Q. Why can mitocho...

Q. Why can mitochondria be considered the power plants of the aerobic cells? Mitochondria are the "power plants" of aerobic cells because within them the final stages of the ce

What organisms make glycogen, What organisms make starch? What is it used f...

What organisms make starch? What is it used for? What organisms make glycogen? What is it used for?

Methods of estimation of energy expenditure and requirements, Define Method...

Define Methods of Estimation of Energy Expenditure and Requirements? There are a variety of methods available to measure human energy expenditure. Knowledge of these methods wi

What is carbon cycle explain briefly, What is Carbon Cycle explain briefly?...

What is Carbon Cycle explain briefly? Life on earth is mainly carbon-based. Most of the material substances that make up living organisms consist of organic compounds of carbon

Invertebrates, Are chaetopterus species coelomate?

Are chaetopterus species coelomate?

Define casein - tests for presence of exoenzymatic activity, Define Casein ...

Define Casein - Tests for Presence of Exoenzymatic Activity? Casein is a major milk protein composed of various amino acids linked through peptide bonds. Extra-cellular enzyme

Respiratory system, 1. Considering the nature of negative-feedback control ...

1. Considering the nature of negative-feedback control and the function of the respiratory system, what effect do you predict that a decrease in CO2 in the internal environment wou

INVERTEBRATES - STRUCTURE & FUNCTION, 1. Write general characters of phylum...

1. Write general characters of phylum mollusca and classify it upto classes, giving at least two examples of each class.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd