Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

Explain phylum mollusca, Phylum Mollusca (47,000 species) The segmentat...

Phylum Mollusca (47,000 species) The segmentation and coelom may not exist. Diversification through modifications of head, foot; mantle and 'radula for feeding. .Visceralorgans

Common disease of sheep, COMMON DISEASE OF SHEEP - 1 .       ANTHRAX ...

COMMON DISEASE OF SHEEP - 1 .       ANTHRAX - Persons working in wool factories may get infection by breathing. 2 .       BLACK QURTER 3 .       HAEMORRHAGIC S

Slow walking or crawling, Slow walking or Crawling This type of locom...

Slow walking or Crawling This type of locomotion is seen while the animal moves on the substratum. It involves a metachronal rhythm of action in the parapodia. Each fifth or

Is fecundation in amphibians external or internal, Is fecundation in amphib...

Is fecundation in amphibians external or internal? In this aspect are amphibians evolutionarily proximal to fishes or to reptiles? In the majority of the amphibian species fec

What is prostaglandins , Prostaglandins and the structurally associated mol...

Prostaglandins and the structurally associated molecules prostacyclins, leukotrienes and thromboxanes, are called as eicosanoids because they having 20 carbon atoms. These hormones

Urolithiasis, Ur o l it h i a s i s The presence of calculi (u...

Ur o l it h i a s i s The presence of calculi (urolith) in the urinary system is termed as urolithiasis, and the disease is characterized by distension of bladder and

What are retroviruses, What are retroviruses? How do they reproduce and wha...

What are retroviruses? How do they reproduce and what is the role of the enzyme reverse transcriptase? Retroviruses are viruses whose genetic material is RNA. HIV and the virus

Explain about the saccharin - artificial sweeteners, Explain about the sacc...

Explain about the saccharin - artificial sweeteners? The oldest artificial sweetener is saccharin (the calcium or sodium salt of 1, 2- benzisothiazol-3(2H)-one 1, 1-dioxide) as

Ttt, respiration.

respiration.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd