Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

What are the features of hnrna, Q. What are the features of hnRNA? Hete...

Q. What are the features of hnRNA? Heterogeneous nuclear RNA (hnRNA) - Primary precursor mRNA transcript made in eukaryotic nucleus are called 'hnRNA,' an abbreviation for 'het

Solution of ampicillin, How much ampicillin (sodium sal, mw=371.40) would y...

How much ampicillin (sodium sal, mw=371.40) would you dissolve in 400 mL of water to make 80 mg/ml solution of ampicillin?

Onset of puberty in female, ONSE T OF PUBERTY IN FEMALE - Attains at t...

ONSE T OF PUBERTY IN FEMALE - Attains at the age of 13 by estrogen hormone. It includes - 1. Growt h of breasts 2. Growth

Endoscopy, E n d o scopy (Greek: endon, within; skopion, to loo...

E n d o scopy (Greek: endon, within; skopion, to look at) Endoscope, a tubular instrument, is used to examine interior of body cavity or a hollow organ.  Besides e

Describe the theory of darwins, Describe the theory of Darwin's? Most s...

Describe the theory of Darwin's? Most scientists today accept the theory that living organisms have evolved according to the concept that was first proposed by Charles Darwin i

Phases of cell cycle, The cell goes through many discrete phases before and...

The cell goes through many discrete phases before and after cell division. From this understanding, scientists then identified the four characteristic phases of the cell cycle:

Explain magnetic stirrer - food microbiology, Explain Magnetic Stirrer - Fo...

Explain Magnetic Stirrer - Food Microbiology It can be used for mixing ingredients at the time of media or reagents preparation. Mixing happens with spinning of a teflon coat

Mass dispersal - population dispersal, Mass Dispersal - Population Dispersa...

Mass Dispersal - Population Dispersal A population is inherently dynamic in nature since individuals are always leaving or entering the populations. But such changes normally

Define chain and ring forms and anomerism, Define Chain and Ring forms and ...

Define Chain and Ring forms and anomerism? Many simple sugars can exist in a chain form or a ring form. The ring form is favoured in aqueous solutions, and the mechanism of rin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd