Tissue culture, Biology

Assignment Help:
Tissue culture
  1. The ability of a plant cell to give rise to a whole plant is called totipotency.
  2. The culture of a cell or a tissue in a suitable medium to produce new plants is called tissue culture. This process is discovered by G. Haberlandt in 1902.
  3. By tissue culture techniques new plants can be obtained from single cells or a tissue or a bud or other organs of the plant.
  4. The portion of the plant that is used in tissue culture technique is called as explant.
  5. The techniques require maintenance of sterile conditions in the medium.
  6. The medium should contain sugars, minerals and one or more growth regulators and are kept free form bacteria and fungi. Explant should be sterilised.
  7. The explant divides to give rise to an unorganised mass of tissue called callus.
  8. Callus undergo differentiation into shoots, roots or embryo like structures called Embryoids. It depends on the concentration and combination of plant growth substances like auxins and kinetin in the medium.
  9. Embryoids when cultured separately develop into new plants.

1306_Tissue culture.png


Related Discussions:- Tissue culture

What are the biological troubles, Q What are the biological troubles that m...

Q What are the biological troubles that molluscs face due to their soft body? Because molluscs have a soft body they are further fragile. They also have more complexity to supp

Show the directly visible chemical characters, Q. Show the Directly Visible...

Q. Show the Directly Visible Chemical Characters? Very few chemical substances in plants can be observed directly, but the few substances such, starch grains which are pres

Use of chemicals to control and destruct microorganisms, Q. Use of Chemical...

Q. Use of Chemicals to Control and Destruct Microorganisms? Ans. The use of chemicals, as a process of food preservation, has been used since long, ever since man found

What is global warming, What is global warming? Global warming is the e...

What is global warming? Global warming is the enhance in the temperature of the planet due to accumulation of some gases in the atmosphere, especially gases that retain the sol

Heliozoans - protozoan, Heliozoans - Protozoan Heliozoans are spherica...

Heliozoans - Protozoan Heliozoans are spherical protozoan that occur in the sea or in still bodies of fresh water. They are mainly located in the bottom debris. Fine needle li

Allele-specific ligation, Allele-specific ligation  is the technique permit...

Allele-specific ligation  is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other

Explain about derived proteins, Derived Proteins These are not naturall...

Derived Proteins These are not naturally occurring proteins and are obtained from simple proteins by the action of enzymes and chemical agents, heat, mechanical shaking, UV or

Procedures for diagnosis - immune responses and biomarkers, Demonstration o...

Demonstration of immune responses and biomarkers: This may be done either on the basis of sero-conversion study on paired sera samples or by challenge (protection) test.

Types of muscular tissue, TYPE S -   There are present three types ...

TYPE S -   There are present three types of muscle fibres:   1.      Striped or striated or skeletal or voluntary muscles.   2.      Unstriped or non-striated o

Do all genetic diseases result no. chromosomes of the cells, Do all genetic...

Do all genetic diseases result from alteration in the number of chromosomes of the cells? Besides aneuploidies there are other genetic diseases, other chromosomal abnormalities

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd