The principle of dna cloning, Biology

Assignment Help:

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduced into bacterial cells the most or all will lack the ability for self-replication and will fast be lost. Furthermore, two kinds of DNA molecule are known that can replicate autonomously in bacterial cells; plasmids and bacteriophages. Plasmids are little circular double-stranded DNA molecules which exist free inside bacterial cells, frequently carry certain genes which confer drug resistance and are self-replicating of the genes.  If a recombinant DNA molecule is establish by joining a foreign DNA fragment to plasmid or bacteriophage DNA then the foreign DNA is replicated when the plasmid or phage DNA is replicated.  In that role the plasmid or phage DNA is known as a vector.  Todays, a population of recombinant DNA molecules can be build, every recombinant molecule having one of the foreign DNA fragments in the original combination.

 


Related Discussions:- The principle of dna cloning

What are the phytoplankton and the zooplankton?, What are the phytoplankton...

What are the phytoplankton and the zooplankton? The Phytoplankton and the zooplankton are divisions of the plankton. The phytoplankton includes the autotrophic floating beings:

Who are a group of lay people without background science, In detail describ...

In detail describe the structure and function of the heart and blood flow. Include the conduction system and blood vessels. Make your response specific to your audience who are a g

Explain xylose isomeras, Explain xylose isomeras A mixture of glucose ...

Explain xylose isomeras A mixture of glucose and fructose, is produced from sucrose by the action of yeast invertase (β-fructofuranosidase), an enzyme which can only be extrac

What is sustainable agriculture, What is sustainable agriculture? Desc...

What is sustainable agriculture? Describe the contribution of biopesticides and biofertilisers in sustainable agriculture.

Define the term - lateralisation and localisation, Define the term - latera...

Define the term - lateralisation and localisation Discriminative validity studies including lateralisation and localisation achieved satisfactory results, but the localisation

Which are the beings that form the kingdom plantae, Q. Which are the beings...

Q. Which are the beings that form the kingdom Fungi? The kingdom Fungi is created by fungi. Q. Which are the beings that form the kingdom Plantae? Are algae parts of this k

A balanced diet must contain enough energy, A balanced diet must have enoug...

A balanced diet must have enough energy to meet the body's needs. What else must it have?    In addition to sufficient energy, a balanced diet must have proteins, car

How would contrast single covalent bonds, 1. In modern blimps, the gas of c...

1. In modern blimps, the gas of choice used to inflate them is helium rather than hydrogen. Hydrogen would be lighter, but helium is safer. Compare and contrast the atomic structur

How to dna fragments for comparison with other dna samples, DNA fingerprint...

DNA fingerprinting produces a unique banding pattern of DNA fragments for comparison with other DNA samples.

Process of behaviour change, 1. Awareness - First people become aware of a...

1. Awareness - First people become aware of a problem. 2. Acquire Knowledge and skills - Next, they gather knowledge and learn new skills. 3. Motivation - At the next stag

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd