The principle of dna cloning, Biology

Assignment Help:

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduced into bacterial cells the most or all will lack the ability for self-replication and will fast be lost. Furthermore, two kinds of DNA molecule are known that can replicate autonomously in bacterial cells; plasmids and bacteriophages. Plasmids are little circular double-stranded DNA molecules which exist free inside bacterial cells, frequently carry certain genes which confer drug resistance and are self-replicating of the genes.  If a recombinant DNA molecule is establish by joining a foreign DNA fragment to plasmid or bacteriophage DNA then the foreign DNA is replicated when the plasmid or phage DNA is replicated.  In that role the plasmid or phage DNA is known as a vector.  Todays, a population of recombinant DNA molecules can be build, every recombinant molecule having one of the foreign DNA fragments in the original combination.

 


Related Discussions:- The principle of dna cloning

Explain radial symmetry in classification animal kingdom, Explain Radial S...

Explain Radial Symmetry in Classification of the Animal Kingdom? All of the animals besides the sponges are considered to belong to the Eumetazoa, or the "true animals" because

Alive and something that is a living organism, Is there a difference betwee...

Is there a difference between something that is alive and something that is a living organism?

Explain about the dna viruses - carcinogenic, Explain about the DNA viruses...

Explain about the DNA viruses - Carcinogenic? The three DNA viruses found to cause human cancers are EBV, HBV and HPV. What are these? Let us find out. Epstein-Barr viru

Define nutritive aspects of food constituents, Define Nutritive aspects of ...

Define Nutritive aspects of food constituents and effect of processing and handling? Nutrient stability; effects on nutrients of agricultural practices, handling, processing an

What is a food allergy (hypersensitivity), What is a Food Allergy (Hypersen...

What is a Food Allergy (Hypersensitivity)? A food allergy or hypersensitivity is an abnormal response to a food by our immune system. As illustrated in Figure, this non-toxic a

Fundamental structure of the hiv virus, Q What is the fundamental structure...

Q What is the fundamental structure of the HIV virus? What is the function of the glycoproteins of its envelope? HIV is an RNA virus in its core there are two strands of RNA an

Phylum protozoa, what are the classfication and members af phylum protozoa

what are the classfication and members af phylum protozoa

explain alternative processing, Alternative polyadenylation sites Exa...

Alternative polyadenylation sites Exact pre-mRNAs hold more than single group of signal sequences for 3' end polyadenylation and cleavage.   In  some  cases,  the  area  of  t

Evolution, Who is the founder of theory survival of the fittest ?

Who is the founder of theory survival of the fittest ?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd