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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
The lithosphere contains all of the hard solid land of the earth crust and the semisolid land underneath the crust. The surface of the lithosphere is very uneven contains high moun
VOLVOX CHARACTERS
Q. Which are the heart chambers respectively where the entrance and the exit of blood occur? The heart chambers through which blood enters the heart are the atria there are the
Chloroplasts is the disk-like organelles with the double membrane found in eukaryotic plant cells; contain thylakoids and are the site of the photosynthesis. ATP is produced durin
What are intraspecific and interspecific ecological interactions? Intraspecific ecological interactions are those among individuals of the same species. Interspecific ecologic
CALCIUM It is the most abundant mineral of animal body. Calcium is available in all types of vegetables, grain, milk, cheese, eggs, fish and butter. By its deficiency r
Q. Show How Adaptation affecting taste quality? Adaptation - A low concentration solution will not give any sensation after tasting a higher concentration solution due to the a
What Devices Used for Oxygen Therapy? Variable performance systems: With these devices, the FiO 2 would vary depending on the patient's breathing pattern. In general, rapid, s
Why do cells of the nephron tubules present a great amount of mitochondria? The cells of the tubule wall have high number of mitochondria because lots of substances are resorbe
Define Iodothyronine Deiodinases - functions of selenium in humans? Another group of selenoproteins are the iodothyronine deiodinases essential for the conversion of thyroxine
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