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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
THYROID GLAND - It develops from the endoderm of the embryo. Th e thyroid gland is the largest endocrine gland located anterior to the thyroid cartilage of the larynx
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Normal or General Diet This diet is planned to be consistent with the Recommended Dietary Allowances (RDAs) of nutrients and is based on the food groups. It is usuall
what are the modes of reproduction?
State about the importance of Potassium Potassium is the third major element required for plant growth, generally absorbed as K + ion. In soil 95 to 99 percent of potassium e
Explain the Food Irradiation - method of food preservation? Food irradiation is another sterilizing technique in which the foods are bombarded by high-energy rays called gamma
Explain about the Niacin (nicotinic acid) deficiency? Niacin (nicotinic acid) deficiency classically results in pellagva, which is a chronic wasting disease associated with a c
A new drug named AGON-CaSR has been developed that is an agonist at calcium-binding sites of CaSRs (Calcium-Sensing Receptors) in plasma membranes of parathyroid gland cells.
Explain Carbohydrates - Nutritions? Liberal amounts of carbohydrates should be given i.e. around 60% to 65% of the total energy. However, care must be taken regarding the maxim
Mixed Aortic Stenosis and Regurgitation : The combined aortic valve stenosis and regurgitation may be of congenital or acquired aetiology, as in aortic stenosis. Most common
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