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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
USES OF DRUGS - 1. Drugs are prescribed as medicines to prevent or cure a variety of diseases. 2. May be used to promot physical and mental health. 3. U
Explain about the Multiple Sclerosis This is a disease characterised by the loss of myelin, largely in motor tracts but also in sensory tracts. The loss of myelin is not unifor
Reproductive System of Asteroidea Sexes are separate in several asteroids. There are ten (10) gonads, two in each arm. They emerge tuft-like or as cluster of grapes. Mature go
What is thigmotropism? Thigmotropism is the movement or growth of the plant in response to mechanical stimuli (touch or physical contact), as when a plant grows around a suppor
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Define Reagents for Estimation of Calcium Using EDTA Titrimetric Method? 1. Buffer solution: Dissolve 16.9 g NH 4 CI in 143 ml NH 4 OH. To this add 50 ml of solution containing
In a follow up to Alfred Sturtevant's studies on recombination in the fruit fly, Seymour Benzer used complementation studies of bacteriophage mutants to verify where recombination
Measuring ecosystem diversity is not easy, and ther e is no definite index for its measurement. It is generally assessed by the measure of species diversity in an ecosystem, often
Effect of Environmental Factors some lower organisms, though the genetic determiners of the sexes are present, the ambient environment plays a decisive role in the development
Budding - Types of Asexual Reproduction In reproduction through budding, small groups of cells form buds in some part or parts of the parent animal. The bud grows, differentia
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