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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Digestion The nutritional requirements and the various ways used by heterotrophic organisms to obtain nutrition. Whether food is used to give energy or to build the body, the
Q. What is the metagenesis? What are the other names of this procedure? Metagenesis is thekind of life cycle in which there are two different forms of individuals of the same s
Fatty acids are synthesized in the cytosol, but acetyl CoA is produce from pyruvate in the mitochondria. Therefore the acetyl CoA must be transferred from the mitocho
Define the Parenteral Nutrition Support? Parenteral nutrition support is the provision of dextrose, amino acids, electrolytes, vitamins, minerals and trace elements with or wit
Nutrients and the Soil Early experiments on mineral uptake were performed by Hoagland, Stout and Amon in 1923. They showed that minerals were taken up from the soil primarily
Q. How do you define Normal Pulmonary Vasculature? Pulmonary vessels are seen in the medial 2/3 of the lung. Vessels are generally not identified in the lateral third. The rad
C o m p o s t i n g It is a modification of a conventional or solid manure handling system with anaerobic decomposition. Types of composting systems include windrow, b
Two true-breeding pea plants were crossed. One parent is round, terminal, and violet, constricted, while the other expresses the respective contrasting phenotypes of wrinkled, axia
SPLEEN Largest lymph gland, also with myeloid tissue is an important specialized reticuloendothelial organ in vertebrates, as the site of erythropoiesis. Splenic tissue i
Q. What are the subkingdoms into which the plant kingdom is divided? The kingdom Plantae is divided into two big subkingdoms: the tracheophytes and the bryophytes (gymnosperms,
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