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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
How the needles are classified by thier point geometry Needles may also be classified by their point geometry; examples include: - taper (needle body is round and tapers smo
How many g of glucose will be required to make 750 ml of a 0.5% w/v solution? Please show all steps.
What is the difference between ectoparasite and endoparasite? Ectoparasites are parasites that explore the external surface of the host (as, for example, mites that parasite th
Casein is the main protein in milk. There are three types of casein, one of which is beta casein. Beta casein comes in two main variants called A1 and A2. The order of amino acids
Q. Who were James Watson, Maurice Wilkins and Francis Crick? Watson (North American), Wilkins (New Zealander) and Crick (British) were the discoverers of the molecular structur
Compared to platyhelminthes which physiological problem have the cylindrical body of nematodes brought? How was that problem solved? The cylindrical shape of nematodes made imp
Incubator The incubators are essential to provide an ideal micro environment. The main functions are: isolation, maintenance of thermo neutral ambient temperature, desired h
Q. Prevention strategy for diverticular disease? The prevention strategy for the disease involves the following: • Eat a high-fiber diet (more than 15 g/day of crude fiber)
Define Gonads and Reproductive System? With aging, there is decrease in pituitary hormone secretion causing decrease in gonadal hormone production, which causes number of chang
Q. Use of echocardiography in pericardial diseases? One of the earliest clinical applications of echocardiography was in the detection of pericardial effusion and it remains th
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