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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Explain Pasteurization (temperature below 100° C) - method of food preservation? Pasteurization is a heat treatment that kills a part but not all the microorganisms present and
Mounting boxes for insect collections Wood or cardboard cigar boxes make very useful and suitable housings for insect collections. After the insect has been separate from the s
what is biodiversity
Q. What is the kind of cell division that allows sexual reproduction? What is gametogenesis? Meiosis is the kind of cell division that permits sexual reproduction since it redu
how can explain
How was it proved in the case of Amoeba that the key to the life of a cell is Nucleus?
#q1. If offspring exhibit a 3:1 phenotypic ratio, what are the genotypes of a parent?uestion..
The common nitrogen-fixer in paddy fields is: 1. Rhizobium 2. Azospirillum 3. Oscillatoria 4. Frankia Azospirillum is the common nitrogen-fixer in paddy fields
Q. What is the relationship between enzyme and vitamins cofactors? Many vitamins are enzyme cofactors that cannot be synthesized by the organism and must be acquired from the d
Caffeine and alcohol both block the secretion of ADH from the posterior pituitary gland. Explain what this does to fluid balance. Does it make sense to drink caffeine before an ath
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