The basics of dna cloning, Biology

Assignment Help:

There  are a broad  range  of various  process  for cloning  DNA  into  either viral  or plasmid vectors  but the basic  scheme  of events  is frequently  same.  To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed.  The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.

 


Related Discussions:- The basics of dna cloning

Oxidation reduction potential, what exactly is oxidation and reduction pote...

what exactly is oxidation and reduction potential and how its an imp. factor in spoiling meat

Explain in detail about respiratory system, Explain in details about Respir...

Explain in details about Respiratory system Respiratory system starts from nostrils through which we inhale air in the nasal cavity. Then, air enters pharynx and goes through l

Briefly explain about the chromium, Briefly Explain about the Chromium? ...

Briefly Explain about the Chromium? As you will go through this section, you will realize that compared to other minerals, the essentiality of chromium was recognized very late

How the molecule would increase in mass slightly, Suppose many of the atoms...

Suppose many of the atoms of nitrogen in a double-stranded molecule of DNA, of isotope N-14, were replaced with the isotope N-15. What afffect would this have? a) The nitrogeneo

Define the interaction of vitamin c with iron, Define the Interaction of Vi...

Define the Interaction of Vitamin C with iron? Ascorbic acid powerfully enhances absorption of non- haem iron and reverses the inhibiting effect of tea and calcium phosphate. I

How is carbon dioxide released by cellular respiration, Q. How is carbon di...

Q. How is carbon dioxide released by cellular respiration transported from the tissues to be eliminated through the lungs? In vertebrates almost 70% of the carbon dioxide is ca

Cardiac veins, Cardiac Veins The anterior cardiac consisting of vesse...

Cardiac Veins The anterior cardiac consisting of vessels covering the anterior right ventricle, I drain into the right atrium. The great cardiac vein drains the blood form

Hardy - weinberg law, HARD Y - WEINBERG LAW - Proposed by G.H. Hard...

HARD Y - WEINBERG LAW - Proposed by G.H. Hardy and W. Weinberg in 1908 independently. The law states "the frequency of genes or alleles in a population remains constant

What is leghemoglobin, The  nitrogenase  complex  is  extremely  sensitive ...

The  nitrogenase  complex  is  extremely  sensitive  to  inactivation  by  O2,  so  the enzyme  must be protected  from this reactive  substance.  During the root nodules of legumi

Describe the working of ploidy reduction, Q. During which meiosis division ...

Q. During which meiosis division does ploidy reduction take place? And Does it ploidy reduction occur in mitosis? In the cell division by meiosis ploidy reduction occurs in mei

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd