The basics of dna cloning, Biology

Assignment Help:

There  are a broad  range  of various  process  for cloning  DNA  into  either viral  or plasmid vectors  but the basic  scheme  of events  is frequently  same.  To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed.  The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.

 


Related Discussions:- The basics of dna cloning

Valvular heart diseases, Valvular heart diseases :  Congenital and acquire...

Valvular heart diseases :  Congenital and acquired valvular heart diseases often require surgical intervention. 1 is well known that conservative surgery by valve repair gives bet

Define Listeriosis monocytogenes, Define Listeriosis monocytogenes L. ...

Define Listeriosis monocytogenes L. monocytogenes can grow over the temperature range of about 1 0 to 45 0 C and the pH range 4.1 to around 9.6, it maybe expected to survive

Rock be well-known from living things, Critical thinking the organization o...

Critical thinking the organization of a rock is much simpler than that of living things. By what other criteria can a rock be well-known from living things? Rocks are not comp

Define measuring body composition- total body potassium, Define measuring b...

Define measuring body composition- Total body potassium? It applies the' concept that body cells contain a constant amount of potassium and that its measurement is theref

State about charles elton, What evidence did Charles Elton collect that sug...

What evidence did Charles Elton collect that suggested that fluctuations in hare and lynx populations were related? What other evidence shows that these fluctuations ma

What is the rule for the pairing of nitrogen-containing base, Q. What is th...

Q. What is the rule for the pairing of nitrogen-containing bases in the DNA molecule? And in the RNA? Is this the last question appropriate? The rule for the pairing of nitroge

What is the minimal space between implants and implant, Minimal space betwe...

Minimal space between implants and implant and adjacent tooth It is important to maintain a distance of at least 1.5 to 2 mm between an implant and adjacent tooth and a distanc

Describe about aorto pulmonary window, Describe about Aorto Pulmonary Windo...

Describe about Aorto Pulmonary Window ? This uncommon malformation consists of communication usually non-restrictive between the adjacent walls of the ascending aorta and pulmo

Compare and contrast enantiomers in relation to sugars, Compare and contras...

Compare and contrast enantiomers, epimers, and anomers, specifically in relation to sugars.

Phylum Echinodermata, define and explain Water vascular system with podia a...

define and explain Water vascular system with podia and tube-feet madreporite mostly present

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd