The basics of dna cloning, Biology

Assignment Help:

There  are a broad  range  of various  process  for cloning  DNA  into  either viral  or plasmid vectors  but the basic  scheme  of events  is frequently  same.  To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed.  The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.

 


Related Discussions:- The basics of dna cloning

Expression of variability, In the previous sub-section we discussed the pos...

In the previous sub-section we discussed the possible ways by which variabiliti can, be generated. We shall now examine one instance that illustrates the consequence of variability

Prototheria, prototherians are unfinished mammals

prototherians are unfinished mammals

Indicator organism, a) What are the needsfor an organism to be an indicator...

a) What are the needsfor an organism to be an indicator organism? b)  In Australia, National Parks and coastal beaches attract various visitors during holiday periods.  EPA is

Inhalation route for injection, Inhalation Route for Injection Therapi...

Inhalation Route for Injection Therapies that can be administered by inhalation are oxygen, humidification-cool mist and steam, administering local medication. The commonly u

ASSINMENT, HISTORY ON CLASSIFICATION IN BIOLOGY

HISTORY ON CLASSIFICATION IN BIOLOGY

Evolution of heart in higher animals, Evolution of heart in higher animals ...

Evolution of heart in higher animals The circulatory system in higher animals has undergone several changes during evolution. The heart has become totally muscular. Th

Simple indigestion, S i m p l e indigestion The disease is common ...

S i m p l e indigestion The disease is common in stall-fed individuals. Consumption of indigestible food material, moldy and nutritionally unbalanced feed results in simpl

Becoming online tutor, how can i work with u as an online biology tutor?

how can i work with u as an online biology tutor?

Taxonomy, . Explain why the traditional classification of unicellular euka...

. Explain why the traditional classification of unicellular eukaryotes as ‘protozoa’ or ‘protists’ is invalid in terms of modern systematics and evolutionary theory. Why are trad

Tell us about three kinds of dna, There are 3 types of DNA sequences. Genom...

There are 3 types of DNA sequences. Genomic DNA comes from the genome and haves both genes and extragenic material. cDNA is reverse transcribed from mRNA and corresponds only

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd