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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Q. From the lumen to the external surface what are the tissues that form the digestive tube wall? From the internal surface to the external surface, the digestive tube wall is
Hemoglobins - Process of Respiration Hemoglobins are the very well known of all respiratory pigments. The basic molecular unit of Hemoglobin contains a haem group bound to a
Q. Function of Dopamine in consciousness? An increase in dopamine activity produces an increase in wakefulness. Dopaminergic neurons in the ventral tegmental areas are constant
Explain the Systemic Metabolic Responses? Many of the metabolic responses to infection are similar to those following injury. The key changes include: Hyper metabolism: Oxyg
Q. Which are the organs of the excretory system? The excretory system is formed of ureters (two), kidneys (two), bladder and urethra.
What are the parts of Nervous system Nervous system is divided into three main parts: 1) The brain and spinal cord or Central Nervous System (CNS). 2) The nerves or Peri
Concerning tissue complexity how different are cnidarians from poriferans? Cnidarians have true tissue differentiation, they present separate organized tissues in the body. Por
What is inflammation? Inflammation is the initial response of the unspecific defense system versus aggressions against the body (the aggressions might be caused by infectious p
Q. In which nephron portion does the regulation of alkalinity and acidity of the plasma occur? The regulation of the acid-basic equilibrium of the body is done by the kidneys a
Explain the Feeding 6-12 Months Infant? As the infant grows older, mother's milk alone is not sufficient to meet his increasing needs. This could be seen in few infants between
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