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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
what is the effect of insuline in body ?
How will the urine concentration be affected if the henle''s loop of a person is removed and his PCT is joined directly with DCT?
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Why have biologists and bioinformaticians embraced the Web as a vehicle for disseminating data so quickly, whereas clinicians and clinical informaticians have been more hesitant to
What is the normal duration of the menstrual cycle? How does the calendar contraceptive method work? The normal duration of the menstrual cycle is 28 days but it can vary betwe
Q. What are the few examples of the structural function of organic molecules? Ans. Organic molecules have a structural function as they are part of cell membranes, organ w
How Genetic Predisposition causing the underweight? Mother's Health Status; Poor nutritional status of the girl child coupled with under nutrition during pregnancy results in L
Explain Subclavian Flap Aortoplasty ? Initial steps are same as for resection and end-to-end anastomosis. After exposing a the entire length of intra thoracic segment of left s
Prepared Assignments on glycolysis
Equine herpes virus infections (equine rhinopneumonitis, abortions) Equine herpes virus infections in horses and foal are caused by Alphaherpes virus of herpesviridae family p
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