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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Q. Write the meaning of Hyperglycemia? Hyperglycemia is a Greek term: hyper -meaning excessive; glyc - meaning sweet; and emia- means "of the blood". It is a condition in whic
How do keep respiratory system healthy? The circulatory system supplies food and oxygen to the body's cell. It carries away waste production of energy. The wastes must then be
Q. Explain H - Shaped Incision? This type of incision is especially useful for: The anterior maxilla or mandible. Accurately identified implant position. Exposure o
Caffeine and alcohol both block the secretion of ADH from the posterior pituitary gland. Explain what this does to fluid balance. Does it make sense to drink caffeine before an ath
Which of the following most accurately explains the meaning of a repression lag? A. A repression lag refers to the time that it takes for the last full-length mRNA transcript t
Acute Glomerulonephritis If you review the anatomy of urinary system you will recall that nephron is the working unit of kidney and its number exists in millions. Within t
Cleavage - Metazoa The unicellular zygote begins cell division (cleavage). First, the single cell divides forming two cells, these redivide further to form four, then eight ce
what are the function of nucleus
• Blood cultures are critical in the diagnosis and management of IE. • Obtain blood cultures before starting antimicrobial therapy whenever possible. • Intravascular infection l
Q. Why pH regulation is important for living beings? How mineral salts participate in this regulation? The prospective of hydrogen (pH) is a measure of the amount of hydrogen i
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