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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
What is polymorphism
Anti-retroviral drugs are given to the patient. They lesser the viral load and gives relief from infection, but it is not lasting it is temporary relief i.e. it cannot cure.
Q. Mammal identity card. How are mammals characterized according to examples of representing beings, skin, respiration, nitrogen waste, basic morphology, circulation, thermal contr
Infraorbital nerve and vessels The infraorbital nerve exits through the infraorbital foramen. This foramen and the neurovascular contents are within 5 to10mm of extremely resor
What would you conclude from PCR/DGGE (denaturing gradient gel electrophoresis) analysis of a sample that yielded one band by PCR and one band with DGGE? One band by PCR and two ba
Explain the work of Linnaeus in animal Taxonomy? In 18th century, the work of Linnaeus and his followers (Haartman, 1751, 1764, Kolreuter, 1761-66) helped systematic to advance
What is visual accommodation? Visual accommodation is the phenomenon of varying the curvature of the crystalline lens to make possible the variation of its refractivity to adju
What is the difference between spermatogonium and spermatocyte I? The male germ cells are the spermatogonia (diploid cells, 2n) located in the testicles. They mature and by mea
SPERM A T OGENESIS The process of maturation of reproductive cells in the testes of male to form spermatozoa (sperm) is known as spermatogenesis. The testes are formed
NONSTRIATED (= SMOOTH) MUSCLES - Non-striated muscles are found in the posterior part of oesophagus, stomach, intestine, lungs, urinogenital tract, urinary bladder, blood ve
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