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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
Explain about natural pigments There has been an extensive search of the microbial, plant and animal kingdoms for pigments that possess both high tinctorial power/strength (a m
pictures for hetrotrophic and autotrophic mode of nutrition
Define Source of Electrons - Growth of Microorganism? Depending upon the source of electrons used by microorganisms, these are known as - a) Lithotrophs (rock eaters) - use
Explain some side effects of carbohydrate loading? Some potential side effects of carbohydrate loading are: Muscle , stiffness Diarrhoea Chest Pain Depres
introduction
Q. Why iodine is vital for human beings? Iodine is a essential chemical element for the proper functioning of the thyroid since it is part of the hormones produced by this glan
State in brief about the Zinc It is concerned with the functioning of sulphydryl compounds, such as cysteine, in regulation of redox potential within the cell. Zinc is somet
Define the Prevention of diseases - Function of Vitamin E? Because of its anti-oxidant function and its role in inhibiting cell proliferation of smooth muscles, vitamin E can b
Types of Organization Chart: There are two important types of organization chart. a) Vertical i.e., from top to bottom. b) Horizontal i.e., from left to right.
N a ture of viruses Viruses vary in their size ranging from 20 nm (200 Ao) to 350 nm (3,500 Ao) and in shapes from spherical to bullet, rod, brick or filamentous forms. Some
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