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There are a broad range of various process for cloning DNA into either viral or plasmid vectors but the basic scheme of events is frequently same. To clone into a plasmid vector the round plasmid DNA is cut with a restriction enzyme which has only a one recognition site in the plasmid. This established a linear plasmid molecule with cohesive stops. The simplest cloning strategy is now to cut the foreign donor DNA with the same restriction enzyme. In the other words, different restriction enzymes can be used, giving that they create the similar cohesive ends. The donor DNA and linear plasmid DNA are now mixed. The cohesive stops of the foreign DNA anneal with the finishs of the plasmid DNA and are joined covalently by DNA ligase.
M a g n etic resonance imaging (MRI): It is in use as a clinical diagnostic tool since 1980. The distribution of hydrogen nuclei (protons), present in cellular wa
How do biodiversity, the total number of living beings and the biomass respectively vary during the ecological succession? Biodiversity, the number of living beings and the bio
What is Dynamic Auscultation in heart surgery? It involves determining the effects on heart sounds and murmurs of various physiological and pharmacological manoeuvres, which al
How do antibody-based tests detect how HIV infection works? After the infection by the HIV the immune system starts the production of antibodies (primary immune response) again
What is the importance of vitamin A The importance of vitamin A is undisputable. You may already be aware about the functions/role of vitamin A in our body. Vitamin A is absol
The Polymerase Chain Reaction Kary Mullis established the technique of the polymerase chain reaction (PCR) in the year of 1983. He won a Nobel Prize for the procedure in the ye
COMMO N EYE DEFECTS - 1 . Glaucoma - Pressure of aquous humour is increased, retina is degenerated. 2 . Presbyopia - Due to non etastic cilliary muscles,
What are holandric genes? The Holandric genes are genes situated in the nonhomologous region of the Y chromosome. Holandric genes condition phenotypes that emerge only in men s
Explain Cardiac Type of TAPVC (Draining into Coronary Sinus) ? Initial steps of the operation are the same as described earlier. The right atrium is opened and the roof of the
SYM P A THETI C NERVOUS SYSTEM These sympathetic chain are connected with ramus comminicause of thoracic & lumber part. Due to this reason this system is called as t
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