Steps of initiation , Biology

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The entire mechanism of protein synthesis in eukaryotes is generally the same as in prokaryotes, with three phases explained as termination, elongation and initiation. Furthermore, there are some significant differences, mainly in during initiation.

?   Whereas a prokaryotic  ribosome  has a sedimentation  coefficient of 70S and subunits of 50S and 30S, a eukaryotic ribosome has a sedimentation  coefficient  of 80S  with  subunits  of 60S and  40S.  The composition of eukaryotic ribosomal subunits is also more difficult than prokaryotic subunits but the function of every subunit is essentially the similar as in prokaryotes.

?   In eukaryotes,  each mRNA is monocistronic  which is, discounting  any subsequent   post-translational  cleavage   reactions   which   should   happen  the  mRNA encodes  a one  protein.  In prokaryotes, various mRNAs are polycistronic which is they encode many proteins.  every coding sequence in a prokaryotic mRNA has its own termination and initiation codons.

? Starting of the protein synthesis in eukaryotes needs at least nine distinct eukaryotic initiation factors (eIFs) compared with the three initiation factors (IFs) in prokaryotes.

?   In the eukaryotes, initiating amino acid is methionine isnot N-formylmethionine as in prokaryotes.

 


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