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Which of the following best explains the reason why DNA ligase is needed to complete the replication of internal chromosomal segments?
A. DNA ligase is able to delete the last nucleotide of the oligonucleotide that is left by RNaseH.
B. DNA ligase is able to incorporate nucleotides into the gap that is left by RNaseH and the exonuclease.
C. DNA ligase is able to obtain the final phosphodiester bond thereby fixing single stranded nicks that are left by DNA polymerase.
D. DNA ligase is capable to relieve the tangles that happen ahead of the replication fork.
I dissolve 4µg of DNA in 1mL of water. How much of this solution would I need in order to add 10ng of DNA in a reaction?
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