Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Enzymes and recombinant DNA technology
Recombinant DNA technology, also called as genetic engineering makes use of a variety of enzymes, particularly restriction endonucleases (from bacteria) and DNA ligases to insert extra genes into cells with the help of vehicles termed 'vectors'. One important group of vectors is 'plasmids', which are small, circular, cytoplasmic molecules of DNA, acting as extrachromosomal genes in bacteria. It is possible to extract and purify plasmids and to insert extra genes into the circles. These altered plasmids can then be taken up again from the medium by the microorganism. Then as the bacterium is grown in culture, the inserted gene will be replicated together with the vector. The production of identical copies is termed as 'cloning.
Another group of vectors are the variants of a bacterial virus known as λ phage. The phage DNA is about 45 kb long, of which the middle third has no role in the infection process and can be replaced by another piece of DNA of about the same length (again by means of restriction endonuclease and ligase enzymes) without affecting the ability of the phage to infect bacteria and be reproduced in abundance. Genes or gene fragments of about 15 kb length can be inserted and cloned in this way, in contrast to the limit of 10 kb when plasmids are used as vectors.
Q. Physiological Characteristics of molds? The physiological characteristics of molds will be reviewed only briefly here and will be discussed in more detail subsequently. Mois
FAT METABOLISM Animals digest and utilize three main types of fatty compounds or lipids neutral or true fats ( triglycerides ), phospholipids and sterols. Endproducts
Hydra – Metazoa The animals of phyla Cnidaria and Ctenophora are made up of only two germ layers: ectoderm which forms the outer covering of the animal and endoderm which is t
Explain Gene Expression - Nutrient Gene Interactions? The last two decades have witnessed tremendous development in our understanding of the cellular processes at the molecular
scientific name of apple
INTRODUCTION : It took just hundred years for cardiac surgery to advance to what it is today. Before 1896, surgeons considered heart to be "inviolable" and it was stated that ope
Hypothesis: The spelling of hypothesis when written as "hypotheses" indicate plural. The hypothesis is a statement or declaration of the expected outcome of a research st
recptors and effectors
Steps for Conversion of food Conversion of food is done in four steps: 1) Ingestion, mastication and swallowing: Ingestion or taking in of food and mastication are done b
Glucose-6-phosphate to Glucose Glucose-6-phosphate to Glucose : Glucose-6-phosphate is converted to glucose by glucose-6-phosphatase which is present in intestine, liver and
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd