Standard invert sugar solution, Biology

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Q. Standard invert sugar solution?

Reagents Required

1) Standard invert sugar solution: Weigh accurately 0.985 g of sucrose and dissolve in 500 ml of water. Add 2 ml of concentrated H2SO4. Boil gently for 30 minutes and keep aside for 24 hours. Neutralize this with Na2CO3 and make the final volume to 1000 ml. 30 ml of this solution contains 0.05 g of invert sugar.

2) Fehling A and Fehling B

3) Methylene blue indicator.

Materials Required

Sample of honey

Burette

Pipette

Conical flask

Beaker

Distilled water


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