Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Fluorosis, Fl u o r osi s Continual ingestion of small amount of f...

Fl u o r osi s Continual ingestion of small amount of fluoride through feed or water leads to fluorosis in animals. The toxic effects are based on amount of fluorine inges

Emboly - mechanism of gastrulation, EMBO L Y - The embolic morphogene...

EMBO L Y - The embolic morphogenetic movements are concerned with the inward migration of prospective endodermal and mesodermal blastomeres from the external surface of blast

Show some fungal diseases transmitted by animal feces, Q. What are some fun...

Q. What are some fungal diseases transmitted by animal feces? Pigeon and Bat feces can carry Histoplasma capsulatum, the fungus agent of histoplasmosis. The infection is transm

Explain the obligatory losses of nutrients, Explain the Obligatory Losses o...

Explain the Obligatory Losses of Nutrients? Obligatory losses of nutrient are defined as 'the losses that occur when an individual is put on a diet free of that nutrient'. For

Lymphatic system - circulation, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Genetics Problems, Andalusian chickens may be either black, white, or gray....

Andalusian chickens may be either black, white, or gray. The gene for black is not dominant over the gene for white, nor is the gene for white dominant over the gene for black. Whe

Protoplasm, physical properties of protoplasm

physical properties of protoplasm

Molecular hydrogen, Molecular Hydrogen N 2 -fixing organisms also prod...

Molecular Hydrogen N 2 -fixing organisms also produce a membrane bound enzyme called uptake-hydrogenase under N 2 -fixing condition. The physiological significance of the pres

Carbohydrates, Carbohydrates Fifty five to seventy per cent of the re...

Carbohydrates Fifty five to seventy per cent of the required energy in animals is derived from carbohydrates. However, fats and proteins can also be broken down and used for

Explain the stage of the cardiac cycle, Q. What is the stage of the cardiac...

Q. What is the stage of the cardiac cycle during which the ventricles are filled? The filling of the ventricles with blood take place during diastole.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd