Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Why immune mechanism of body of a patient gets suppressed, Name the disorde...

Name the disorder in which the immune mechanism of the body of a patient gets suppressed. Which pathogen is responsible for it? Give any two reasons of its transmission.

Explain some effect of inadequate nutrition, Explain some Effect of Inadequ...

Explain some Effect of Inadequate nutrition? Inadequate nutrition ranks as one of the major problems of old age. Various factors which may be responsible for the change in one

Botany, tell me best topic related to botany

tell me best topic related to botany

What are the functional divisions of the nervous system, What are the funct...

What are the functional divisions of the nervous system? Functionally the nervous system can be separated into the somatic nervous system and visceral nervous system. The so

Determine the chance of developing cancer, A typical chest x-ray exposes th...

A typical chest x-ray exposes the patient to a radiation dose of 0.01 rem. If a man receives 10 such x-rays in a lifetime, what is his chance of developing cancer as a result of th

History of psychiatric nursing, History of Psychiatric Nursing: In 187...

History of Psychiatric Nursing: In 1873, Linda Richards, developed better nursing care in psychiatric hospitals, and organized nursing services and educational programmes  in

Estimation of protein by carcass nitrogen analysis method, Define Estimatio...

Define Estimation of protein by Carcass nitrogen analysis method? Carcass nitrogen analysis: This method includes Nitrogen retention method Net protein utilization

Explain about skeletal muscle, Explain about Skeletal muscle These are ...

Explain about Skeletal muscle These are attached to the bones by tendons, which help in the movement of the part of the skeleton. These muscles are controlled by our conscious

Advantages and disadvantage of using turbidity measurement, Advantages and ...

Advantages and Disadvantage of using Turbidity Measurement? Advantages 1. It is an easy, rapid and sensitive technique. 2. Turbidity measurement can be done without destr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd