Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Zoonoses disease-yabapox, Yabapox Yabapox is a disease affecting mangabeys...

Yabapox Yabapox is a disease affecting mangabeys, rhesus, cynos, vervets, stumptails, and patas monkeys. It is caused by the Yaba-like disease virus (YLDV) and Yaba monkey tumor v

PROTOZOA., WHAT ARE THE ADVANTAGES & THE DISADVANTAGES OF PROTOZOA?

WHAT ARE THE ADVANTAGES & THE DISADVANTAGES OF PROTOZOA?

Ventilation of gills, Ventilation of Gills Various mechanical devices ...

Ventilation of Gills Various mechanical devices are used to increase the flow of water over the gill's surface. There can be two means of increasing the flow of water over the

What are enzymes?, Enzymes are proteins that are catalysts of chemical reac...

Enzymes are proteins that are catalysts of chemical reactions. From Chemistry, it is called that catalysts are non-consumable substances that decrease the activation energy essenti

Defination and use of stem cell, Stem cell research has been promoted as a ...

Stem cell research has been promoted as a future medical cure-all for a host of diseases, including Alzheimer's and diabetes, as well as a potential breakthrough for organ replacem

Importance of compliance, It is important to explain to the patient about i...

It is important to explain to the patient about importance of taking treatment regularly. In case he misses the dose or takes medicine or insulin irregularly, explain the side effe

Fundamental cardiac unit, The fundamental contractile unit is the sarcomere...

The fundamental contractile unit is the sarcomere. Each muscle cell apart from the nucleus, mitochondria and other structures contains parallel fibrils. Sarcomere is a subunit of t

Why urea is known to denature proteins, Urea is known to denature proteins ...

Urea is known to denature proteins Based on the structure of urea, how do you think this occurs?

State the active hyperemia, Which of the following is true for active hyper...

Which of the following is true for active hyperemia, a local control mechanism within the circulatory system? A. There will be a decrease in force developed by smooth muscles s

Individuals with tyrosinenmia, Individuals with tyrosinenmia have food patt...

Individuals with tyrosinenmia have food patterns very similar to the one for PKU discussed above. PKU is more common than tyrosinemia. Foods that are high in tyrosine and phenylala

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd