Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Explain epididymitis, Epididymitis  Acute epididymitis in men less than...

Epididymitis  Acute epididymitis in men less than 35 years old is usually caused by C. trachomatis or, less frequently, N. gonorrhoeae. Older men or those who have had urinary

Most important allosteric effector of glycolysis in liver, Name the most im...

Name the most important allosteric effector of glycolysis in the liver. Fructose-2,6-bisphosphate  is the most important allosteric effector of glycolysis  in the liver

What is polyembryony, Q. What is polyembryony? Polyembryony is the phen...

Q. What is polyembryony? Polyembryony is the phenomenon in which a single embryo in its initial embryonic stage divides itself forming many new individuals of genetically ident

Explain medical nutrition therapy, Medical Nutrition Therapy Medical Nu...

Medical Nutrition Therapy Medical Nutrition Therapy  (MNT) is defined as  the  assessment of  the  nutritional status of  a client  followed  by  nutrition  therapy ranging Pon

Explain the carbohydrates and fat requirements during sepsis, Explain the C...

Explain the Carbohydrates and Fat Requirements during Sepsis? Carbohydrates should constitute nearly 60% to 70% of the total energy. Glucose is the primary calorie substrate in

What is the mechanism of gap junctions, What is the mechanism by which gap ...

What is the mechanism by which gap junctions could relay signals from G protein coupled receptors between cells within a tissue?

Tricuspid annuloplasty-types of surgery, Tricuspid Annuloplasty :  Tr...

Tricuspid Annuloplasty :  Tricuspid valve is evaluated by digital palpation before connecting the patient to cardio pulmonary by pass. Functional regurgitation can be repaire

Describe the structure of lipoprotein, There are several different lipoprot...

There are several different lipoprotein species found in plasma but their basic structures are similar. The insoluble lipid (cholesterol ester and triglyceride) forms a central cor

Discuss in brief about maxillary sinus, Discuss in brief about maxillary si...

Discuss in brief about maxillary sinus In normal conditions, the maxillary sinus maintains a close relationship with the alveolar ridge associated with second premolar and fir

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd