Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Tr aditional Meat Products The rich heritage of India contributes to wide range of traditional foods and has a role to play in health foods. Indigenous meat products are uniqu
What are respectively some remarkable functions of myosin, CD4, albumin, keratin, immunoglobulin, reverse transcriptase, hemoglobin and insulin? Myosin is a protein that when a
Determine the Clinical use of brain imaging in disease management Clinical use of brain imaging in disease management has also grown, driven by broader availability of scannin
Determine some Common Micronutrient Deficiencies? Vitamin A deficiency Iron deficiency anaemia Iodine deficiency disorders Zinc deficiency
VITAMIN - K Also known as naptho quionone / anti haemorrhagic vitamin / coagulation vitamin / menadone . It requires for formation of blood clotting factor prothrombin.
How can the concept of recombination frequency be used in genetic mapping? Genetic mapping is the determination of the location of the genes in a chromosome. By determining
Q. What is the major evolutionary innovation presented by nematodes? What is the benefits of that innovation? The major evolutionary innovation of nematodes is the complete dig
What are the excretory organs in protozoa.
Types of Ovules Anatropous This is the most common type of ovule in angiosperms in which the ovule undergoes curvature such that the micropylar end comes to lie parallel
Chemical Methods of Estimating Available Nutrients Water soluble forms of nutrients are available to plants but forms other than water soluble may also become available to pla
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd