Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Find out which allele is dominant, Diagram the cross and determine which al...

Diagram the cross and determine which allele is dominant for each of the following Drosophila matings? a. Two red-eyed flies yield 110 red-eyed and 35 brown-eyed offspring.!

Define the functional properties of proteins, Define the Functional Propert...

Define the Functional Properties of Proteins? a) Hydration properties (dependent on protein-water interactions), which include properties like swelling, adhesion, dispersibilit

Protozoa., brief account on classification and general characters of protoz...

brief account on classification and general characters of protozoa

General procedure of titration - nutritional biochemistry, Define General ...

Define General procedure of titration - Nutritional  Biochemistry? In general a titration requires that we add precise amounts of the known titrant to the unknown analyte or ti

What is the usual reproduction cycle of a dna virus, Q What is the usual re...

Q What is the usual reproduction cycle of a DNA virus? A usual virus has proteins on its capsid that bind to the outer membrane of the host cell. In the place where the virus a

Describe the term - near-far problem, Describe the term - Near-far problem ...

Describe the term - Near-far problem Signals closer to the receiver are received with less attenuation than signals farther away. Given the lack of complete orthogonality, the

Responsible for the regulation of blood glucose, The pancreas produces insu...

The pancreas produces insulin, a hormone that is specifically produced in the beta cells of the pancreas and it is responsible for the regulation of blood glucose. After carefull c

Which is not produced by the hypothalamus, Which of the following is NOT pr...

Which of the following is NOT produced by the hypothalamus? A) releasing hormones B) inhibiting hormones C) antidiuretic hormone D) oxytocin E) thyroid-stimulating h

What is termination , In the eukaryotes, eukaryotic release factor eRF...

In the eukaryotes, eukaryotic release factor eRF-1 recognizes all three termination codons that are UGA UAA, and UAG and, with the help of protein eRF-3, terminates translation.

What is coronary bypass graft, Q. What is coronary bypass graft? The Co...

Q. What is coronary bypass graft? The Coronary artery bypass graft is a kind of surgical myocardial revascularization that is a way to provide blood to a myocardium whose blood

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd