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This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
As coenzymes participate in a variety of functions, they can be classified broadly into two groups: i) Hydrogen transferring coenzymes, and ii) Group transferring coenzym
How does the study of fossils strengthen the theory of evolution? The study of fossils reveals ancient and extinct species having many same structures to others of the present
List and briefly describe the mechanisms that prevent acid-base imbalances from appearing in the body. In addition, indicate where each mechanism functions most significantly.
Explain what is Class Osteichthyes ? Class Osteichthyes takes its name from the Greek root "osteo," referring to bone, and "ichthys," referring to fish. The bony fish have a sk
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Euglena
What is lactic acid
the appendages of arthropods; a. may serve as walking legs, b. may be modified into atennae, c. may be modified into large pincers, or d. all of the above
Q. What is the name of the cells capable of making gametes? What is the ploidy of these gamete-forming cells? The cells that form gametes are the germ cells as opposed to the s
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