Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Antibiotic resistance is the resistance conferred to the host the ability to survive a given antibiotic by the plasmids containing resistance genes. If such kind of plasmid is pre
structure of rephron
What is reproduction?
"Height" is an example of a gene, but "Short" is an example of...
Q. What are the vitamins which make up the B complex? Which problems does the lack of these vitamins cause? Vitamins of the B complex are: vitamin B1 or thiamin, riboflavin, or
Q. Show the Regulation of blood pressure? Nervous System control of BP Maintains primarily an adequate systemic BP, and alters blood distribution in body to meet specific fu
write the general account of porifera
When the standard free energy of a reaction is -5.7 kj/mol then at equilibrium a- there will be 10 times more products than reactants b-there will be 10000 times more product
how to do assignment on alimentory canal
Artificial Inspiration : a. After artificial expiration, the rescue worker should slowly release the pressure on the back of the victim. b. This will cause the chest cavity t
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd