Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Explain the Functions of Folate? Folate, also known as folic acid, is essential for good health. Folate requiring reactions include those involved in phases of amino acid metabol
Q. How to Use a Key? The use of a key is analogous to travelling a high way, that forks repeatedly, each fork having roadside directions. If a traveller follows the proper dire
Is there a difference between the initial and the final energy levels in catalyzed and non-catalyzed reactions? The catalysis does not alter the energetic state of reagents and
Q. What are the endocrine glands that regulate sexual activity in males? How does this regulation work and what are the involved hormones? In males the sexual activity is regul
Planning the Nursing Care The goals of nursing care are: Identify the causative factor of megaloblastic anaemia. Administer appropriate vitamins depending on
Q. Which are the most important inorganic molecular substances for living beings? Ans. The most important inorganic substances for living beings are mineral salts, water,
Q. What are the main interspecific ecological interactions? The major harmonious interspecific ecological interactions are: protocooperation, commensalism and mutualism. The ma
What are the cell types that form the phloem? What are the main features of those cells? The major cells that form the phloem are the sieve elements and the companion cells. Th
Describe the monomer unit of a protein and how monomer units are assembled into peptides.
Indications for Surgery: In a mixed lesion, either regurgitation or stenosis may be dominant and decision of surgery depends on the haemodynarnics. At the time of surgery on othe
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd