Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
What are the major respective constituents of cell walls in bacteria, protists, fungi and plants? Ans) In bacteria cell wall is made of peptidoglycans; between protists algae ha
describe the biting mechanism of a poisonous snake
What are some examples of biological activities in which osmosis plays an important role? Hemolysis (destruction of red blood cells) by entrance of water, the hydric regulation
What is the role of heart in human body? The heart is the main pump that circulates the blood and fluid within the body. The heart, beats at 72 times per minute, and pumps abou
Describe the access in heart disease Newborn? A secure peripheral or central intravenous access is very essential. Inotropic agents with vasoconstrictor properties like Dopamin
A large number of plants are used for bio-pesticides and bio-fertilizers. Several plant species are used as insecticides against aphids and larvae of insect pests: for insects like
OTHE R GLANDS - (i) Mammary glands - Study of mammary gland is mastology. Modified sweat gland. 4-5 pairs in rabbit. 1 pair in human. In opposum 25 pairs (max.).
Explain the neuritic type of infantile beriberi? It is also referred to as Wernicke korsakoff syndrome or cerebral beriberi. It shows typical manifestations of peripheral neuro
What is Fern Allies - Equisetum ? The earliest land plants are thought to have evolved from green algal ancestors, since they share many features. Both green algae and plants h
how they reproduce?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd