Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

The two regulation systems of glycogen metabolism, Mention the two regulati...

Mention the two regulation systems of glycogen metabolism. There is a hormonal regulation system functioning  in the muscle and liver which regulates  the glycogen metabolism.

Clothes , Clothes : The concept of clothes might have started even befo...

Clothes : The concept of clothes might have started even before weaving, as an extension of  the practice of carrying food hd  implements about.  Attachments with a convenient

Which cannot react with dehydration reaction, Which of the following pairs ...

Which of the following pairs of functional groups CANNOT react with each other by a dehydration reaction? Select one: a. Carboxyl;Hydroxyl b. Carboxyl;Sulfhydryl c. Pho

What happens to the tsh blood level in hypothyroidism, What happens to the ...

What happens to the TSH (thyroid-stimulating hormone) blood level in hypothyroidism? Why is there enlargement of the thyroid in the endemic goiter disease? When there is low T3

Angiographic quantification, Angiographic Quantification Gr 1 - minimal...

Angiographic Quantification Gr 1 - minimal systolic jet, clears rapidly Gr 2 - partial RA opacification Gr 3 - Opacification of whole of RA GR4 - Opacification of

Use of masks as personal protective equipment, Q. Use of Masks as personal ...

Q. Use of Masks as personal protective equipment? Masks are to be worn when procedures that result in aerosol production are performed. Protection from these masks only affords

Classification of hypertension, Q. Show basic Classification of hypertensio...

Q. Show basic Classification of hypertension? Unlike many other diseases, hypertension develops insidiously (without symptoms). In 90% of hypertension cases, the cause is unkn

Define nutritional requirements for young and older children, Define Nutrit...

Define Nutritional requirements for Young and Older Children? Young children need 3 en%, which would be easily met from 8-10 g of oil. However, more visible oil is needed to im

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd