Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Explain about the glycoproteins, Explain about the Glycoproteins? Most...

Explain about the Glycoproteins? Most of the naturally occurring conjugated proteins are glycoproteins. Sugar molecules are covalently bound to them, especially those secreted

Bacteria - type of microorganisms, Q. Bacteria - type of microorganisms? ...

Q. Bacteria - type of microorganisms? Many of us know bacteria only as "germs," invisible to naked eyes that can invade our bodies and make us sick. Few know that many bacteria

Determine the osseus defects around implants, Osseus defects around implant...

Osseus defects around implants. They are classified into four main groups. Group I defects: These demonstrate moderate horizontal bone loss with a minimal intrabony component

How to calculate the raw score for each scale, How to calculate the raw sco...

How to calculate the raw score for each scale The raw score for each scale is the sum of the 0, 1 and 2 item scores. Thus, the higher the score, the poorer the performance. Th

Briefly describe about caramelization, Briefly describe about Caramelizatio...

Briefly describe about Caramelization? Caramelization results from the action of heat on sugars at about 175º C. At high temperatures, sugars dehydrate, break down and polymeri

What is the impaired healing, Impaired Healing and Infection Because of Imp...

Impaired Healing and Infection Because of Improper Flap Design The oral field in itself is a contaminated area due to the presence of the normal oral microflora. Improper flap

Define the term - stimulus, Define the term - Stimulus Stimulus and res...

Define the term - Stimulus Stimulus and response characteristics of the tests themselves, as well as of the test instructions, become exceedingly important considerations. In g

Pathophysiology of chronic wasting disease, Q. Pathophysiology of Chronic w...

Q. Pathophysiology of Chronic wasting disease? We all know that heart attack i.e. myocardial infarction is not the beginning but a last stage representing acute clinical manife

Explain the reduction in cancer risk, Explain the Reduction in cancer risk?...

Explain the Reduction in cancer risk? Different cancers, especially colon and breast cancer, has been linked not only to phytates but also to protease inhibitors. In vitro stud

Systemic diseases in animals, S y s te m i c Diseases Diseases o...

S y s te m i c Diseases Diseases of the gastrointestinal tract The diseases of gastrointestinal system are manifested by abnormality in prehension, mastication and s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd