Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

The cell cycle, what happens to the liver cell during the cell cycle

what happens to the liver cell during the cell cycle

In how many parts minerals are divided, Classification of Minerals Mine...

Classification of Minerals Minerals can be separated into two main categories, namely, main elements (calcium, potassium, phosphorous, chlorine, sodium, magnesium) and trace el

Use of sulphur dioxide and sulphites in microorganisms, Q. Use of Sulphur d...

Q. Use of Sulphur dioxide and sulphites in microorganisms? Sulphur dioxide as a preservative in wine preparation has been in use since ancient times. It is also used in other f

Explain the bio availability of vitamin a, Explain the Bio availability of ...

Explain the Bio availability of Vitamin A? By now it is clear that vitamin A is supplied in two forms. One form is retinol, from animal foods such as liver, fatty fish, eggs, a

Determine about benefits of jogging, Determine about benefits of Jogging ...

Determine about benefits of Jogging Jogging gives a sense of wellness to a diabetic patient. The frequency of jogging should be at least 3 days in week with a distance of 2 km

Fungal enzymes, Hello, I''m doing a...

Hello, I''m doing an assignment on characteristics and types of fungal enzymes and have also been requested to describe their assay.My libra

Definition of counselling, Q. Definition of Counselling? Counselling ha...

Q. Definition of Counselling? Counselling has been defined as a process of helping/enabling a person/people solve certain interpersonal (not able to continue relationship), emo

Protein synthesis with dna, Protein Synthesis with DNA The central dogm...

Protein Synthesis with DNA The central dogma of modern biochemistry is totally based on the coded information holded within deoxyribonucleic acid (DNA). Double stranded DNA is

Which type of gamete produced by a heterozygous individual, Which is the ty...

Which is the type of gamete produced by a heterozygous individual? What is the genotypical proportion of these gametes? Heterozygous individuals, for instance, AA, produce two

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd