Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Other Complications of Prosthetic Valves : One of the dreaded complications of mitral valve replacement is ventricular rupture. It is difficult to manage and so it is better avoi
What is the difference between water-soluble and fat-soluble vitamins? Why can fat-soluble vitamins cause harm when ingested in excess? Water-soluble vitamins are those soluble
Q. Systolic dysfunction onset of symptoms? In asymptomatic patients with normal LV function, the rate of progression to symptoms and/or LV Systolic dysfunction is about 4 per c
Alternative polyadenylation sites Exact pre-mRNAs hold more than single group of signal sequences for 3' end polyadenylation and cleavage. In some cases, the area of t
Locomotion in Arthropoda The Arthropods are characterized by the existence of some special features which can be considered key to their success. These involve a rigid exoske
What are the main functions of the blood? The blood is a means of substance transportation all by the body. The blood distributes nutrients, oxygen, antibodies, hormones, and c
Of which cells is the nervous tissue constituted? How is the generic function of this tissue related to the characteristics of the main cell type that forms it? The nervous tis
Briefly Explain about the Chromium? As you will go through this section, you will realize that compared to other minerals, the essentiality of chromium was recognized very late
Q. Can you define termination of the stress Test? Test should be terminated when: 1) PVCS develop in pairs or with increasing frequency or when ventricular tachycardia devel
What would be the approximate number of amino acids of a protein with a mass of 203 kd? Assume an average molecular weight for amino acids of 110 grams/mol.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd