Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Explain the the use of soy protein in food industry, The use of soy protein...

The use of soy protein concentrates in different applications in food industry is highlighted herewith. a. Bakery products Unlesss higher protein fortification leve

Storage and distribution - sterilization process, Packs, pouches, and casse...

Packs, pouches, and cassettes are sometimes wet after completing the sterilization process. This is a problem usually associated with the use of paper or paper/ plastic wraps in t

Why are vaccines used in the prevention, Q. Why are vaccines used in the pr...

Q. Why are vaccines used in the prevention but not in the treatment of infections? Why can antivenom serums be used in prevention and treatment? Vaccines are not used in the tr

What do you mean by digestive enzymes, Q. Besides the pancreatic juice in t...

Q. Besides the pancreatic juice in the intestine there is the releasing of the enteric juice that contains digestive enzymes too. What are these enzymes and which type of molecule

How does fecundation occur in angiosperms, After the pollination how does f...

After the pollination how does fecundation occur in angiosperms? In these plants is fecundation dependent on water? After the pollination one of the sperm nuclei from the polle

How many chromosomes does a human normal haploid cell have, Q. How many chr...

Q. How many chromosomes does a human normal diploid cell have? How many chromosomes does a human normal haploid cell have? How many are the sex chromosomes within each of them?

Implementation of nursing care, Implementation of Nursing Care   Admin...

Implementation of Nursing Care   Administration of Appropriate Drugs  If  the surgery is not necessary or if  it is postponed for some time then diuretics and digoxin are

What are the changes in the blood count, Many disease states are heralded b...

Many disease states are heralded by changes in the blood count Leukocytosis can be a sign of infection. Thrombocytopenia can result from drug toxicity. Pancytopenia is ge

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd