Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Photosynthesis Photosynthesis - the process by which plants utilise energy from sunlight to convert carbon dioxide and water for the synthesis of sugar. This sugar can then be
Name the alloys which are commonly used The most commonly used alloys are Ti-6Al-4V and Ti-6Al-4V extra low interstitial (ELI). Commercially pure Titanium comes in different gr
If dimples may be considered a single-gene dominant trait, and 64% of the population features dimples, calculate the frequencies of heterozygotes and both types of homozygotes, ass
Change of Health Status Over Time Health of a nation can be gauged from the available information on death.Disaggregated data by causes of death is more reflective of the status
Explain Precautions for Isolation of Pure Culture? 1. Plating of the medium should be done 24 hours before performing the streaking. There should not be any moisture on the
Explain about the Micronutrient Deficiencies? Micronutrient deficiencies are more common during calamities. Can you guess, why? This is due to the lack of diversified food item
Explain the Urinary System in human body? The urinary system removes the waste materials produced as a result of all the metabolic process required to sustain life. The process
Explain Diet history and nutrient intake Diet history and nutrient intake : This is used to evaluate diet for nutrient or food intake. Common methods used include the 24-h
These labs discuss the principle of osmolarity, which is defined as the total number of solute particles in 1 L of solution. Is this the same thing as tonicity? Explain your answer
Q. Why is rubella during gestation a threat to the fetus? If taking place during gestation rubella is a dangerous disease because the virus crosses the contaminates fetus and t
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd