Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Define nutritional requirements of fats and oils for adults, Define Nutriti...

Define Nutritional Requirements of Fats and Oils for Adults? A desirable amount of a linoleic acid to be consumed by a normal adult is 3 en% (ICMR, 1990). The invisible fat pre

Define streptomycin, Define Streptomycin Streptomycin  causes ototoxici...

Define Streptomycin Streptomycin  causes ototoxicity (usually vestibular disturbance) and, less frequently, renal toxicity.  Amikacin  and kanamycin  commonly cause tinnitus an

What do you mean by linnaeus binomial system, Q. What do you mean by Linnae...

Q. What do you mean by Linnaeus binomial system? Almost a century after Bauhin the great Swiss naturalist Carolus Linnaeus (1707-1778) published the two monumental works the "S

What is phosphorylation, What is phosphorylation? What are some biological ...

What is phosphorylation? What are some biological processes in which phosphorylation plays a critical role? Phosphorylation is the name given to processes of the addition of ph

Meiosis, Meiosis is the special type of cell division in which the numbers ...

Meiosis is the special type of cell division in which the numbers of chromosomes in daughter cell are reduced to half, as compared to parent cell. Occurrence: It takes place

What are natural active immunization, What are natural active immunization ...

What are natural active immunization and artificial active immunization? Natural active immunization is that in which a last natural infection induces the primary immune respon

Class turbellaria, plz answer me how I arrange my topic with its order ?

plz answer me how I arrange my topic with its order ?

Describe the maturity stages in fish, Question 1: (a) Describe the mat...

Question 1: (a) Describe the maturity stages in fish. (b) "k" and "r" selection strategies are commonly used to describe the response of fish to biotic and abiotic cond

National health service systems, Normal 0 false false false...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd