Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Define feeding and nutritional management of spinal trauma, Define the Feed...

Define the Feeding and Nutritional Management of spinal trauma? The main objectives of nutritional management are to meet the nutritional needs of the initial acute phase and

Which structures of the body have more bones, Q. Which structures of the bo...

Q. Which structures of the body have more bones for their size than any other part of the body? Hand and wrist have more bones in them for their size than any other part of bod

What are the male pronucleus and the female pronucleus, Q. What are the mal...

Q. What are the male pronucleus and the female pronucleus? The female pronucleus is the proper haploid nucleus of the egg cell and Male pronucleus is the haploid nucleus of the

Bacterial diseases-pigs, Pigs This is a sub-acute or chronic infection ...

Pigs This is a sub-acute or chronic infection manifested by abortion, sterility, high piglet mortality and orchitis in pigs. Br. suis causes brucellosis among pigs. It is morph

Explain the serial dilution process - plate counts, Explain the Serial Dilu...

Explain the Serial Dilution Process - Plate Counts? Dilution factor is the reciprocal of the dilution used. As number of microorganisms in original sample is not known, the ori

Rehabilitation phase for nutritional treatment -neuro trauma, Rehabilitatio...

Rehabilitation Phase for nutritional treatment - Neuro Trauma? Once the patient stabilizes and starts recovering, be sides nutritional replacement, there is a need to assess fu

How to investigate mitral stenosis by electrocardiogram, Q. How to Investig...

Q. How to Investigate mitral stenosis by Electrocardiogram? Electrocardiographic changes are not specific for mitral stenosis and are never diagnostic for any valvular heart di

How establish a pure-breeding population of brown pigs, Would it be possibl...

Would it be possible to establish a pure-breeding population of brown pigs with a few black spots?

Structure and function of lipoproteins, Triacylglycerols, cholesterol and p...

Triacylglycerols, cholesterol and phospholipids are associatively insoluble in aqueous solution. Thus, they are transported around the body in the blood as parts of lipoproteins.

Define foods effect on drug transport, Define Foods Effect on Drug Transpor...

Define Foods Effect on Drug Transport? Many drugs are transported in blood bound lo plasma proteins. Severe malnutrition or diseases affecting the synthesis of plasma proteins

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd