Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

How many are the known amino acids, How many are the known amino acids that...

How many are the known amino acids that form proteins in living beings? There are twenty dissimilar known amino acids that form proteins related to the genetic code of the livi

Breifly describe the menstrual cycle, Breifly describe the Menstrual Cycle?...

Breifly describe the Menstrual Cycle? Each month during a female's reproductive years, the hypothalamus secretes a hormone that stimulates the anterior lobe of the pituitary gl

Functions of the pancreas, The pancreas performs digestive and hormonal fun...

The pancreas performs digestive and hormonal functions. The enzymes secreted by the exocrine tissue in the pancreas help in the break down of carbohydrates, fats, proteins, and

Results-pulmonary valve disease, Results :  In critical pulmonary stenosis...

Results :  In critical pulmonary stenosis in infancy, surgical mortality is 6 per cent. For children and adults with isolated pulmonary valve stenosis, the mortality approaches 0

What is a homologue, Single chromosome of a pair is known as homologue.In H...

Single chromosome of a pair is known as homologue.In Homologous pair two identical chromosomes are there. Each one chromosome in a Homologous pair is known as Homologue.

What is inflammation, What is inflammation? Inflammation is the initial...

What is inflammation? Inflammation is the initial response of the unspecific defense system versus aggressions against the body (the aggressions might be caused by infectious p

Role of a nurse in legal psychiatry, ROLE OF A NURSE IN LEGAL PSYCHIATRY: ...

ROLE OF A NURSE IN LEGAL PSYCHIATRY: 1)   Standard Care : The nurse must function at laid down standards and keep up with the standard of  care by  knowing the policies  & pro

Explain bone healing process in nutritional care, Explain Bone Healing Proc...

Explain Bone Healing Process in Nutritional Care? Protein is essential for callus formation, calcification and bone healing especially in cases of orthopaedic surgery. The prot

What is the respective importance of water, What is the respective importan...

What is the respective importance of water, carbon and nitrogen for living beings? Water is the major solvent of living beings and it is essential practically for all biochemic

Of which substances is chromatin made, Of which substances is chromatin mad...

Of which substances is chromatin made? Chromatin is made of DNA molecules associated to proteins known as histones. Cell Nucleus Review - Image Diversity: chromatin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd