Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Define role of public nutritionist in health care delivery, Define the Role...

Define the Role of Public Nutritionist in Health Care Delivery? It is clearly evident from the foregoing discussion that nutrition is an important, though not the only, determi

Residue in foods, Residue is defined medically as the solid contents that h...

Residue is defined medically as the solid contents that have reached the lower intestine. A low residue diet is composed of foods, which are easily digested and readily absorbed, r

In which field dietetics related, The field of  dietetics can be related to...

The field of  dietetics can be related to: Nutrition care and intervention focused on the individual, and Nutrition care and intervention focused on the group.

Back cross, BACK CROSS When F 1  individual (Tt) is crossed with one of...

BACK CROSS When F 1  individual (Tt) is crossed with one of the two parents, either tall (TT) or dwarf (tt), such a cross is called the back cross. There are two possibilities

Photosynthesis, how do i prove in a diagram that water is needed for photos...

how do i prove in a diagram that water is needed for photosynthesis

Voluntary movements of the big toe of the right foot, Which of the followin...

Which of the following is true for a toe corticospinal interneuron that produces action potentials during voluntary movements of the big toe of the right foot? A. Its dendrites

Elastic fibers of the connective tissue, Q. Of which substance do elastic f...

Q. Of which substance do elastic fibers of the connective tissue are made? What are few functions of these fibers? The elastic fibers are made of a protein called as elastin.

Structure of proteins, STRUCTUR E OF PROTEINS Proteins which are mixed...

STRUCTUR E OF PROTEINS Proteins which are mixed polymers of amino acids can have up to four structural levels - Primary, Secondary, Tertiary, Quaternary 1 .       PRIMA

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd