Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Signify the term - trachea, Signify the term - Trachea Part of the resp...

Signify the term - Trachea Part of the respiratory system in insects, vertebrates and some spiders. In invertebrates it comprise tubules carrying air directly from outside to t

Explain precautions for performing completed coliform test, Explain Precaut...

Explain Precautions for Performing the Completed Coliform Test 1. Aseptic conditions should be adopted while performing the experiment. 2. Destaining is the critical step in

Explain acid and bases, Explain acid and bases? Acids and Bases :  So...

Explain acid and bases? Acids and Bases :  Some aqueous solutions, however, such as HCl, form an excess of hydrogen ions [ H + ] and are called acids. Others, such as NaOH,

Diet, heating a food sample with Benedict''s solution is a test for...

heating a food sample with Benedict''s solution is a test for...

Determine the gymnosperm abundance noteworthy, In which areas of the globe ...

In which areas of the globe is gymnosperm abundance noteworthy? These plants are the typical vegetation of cold regions as the taiga, or boreal forest, of the northern hemisph

Explain resistance to isoniazid, Resistance to Isoniazid  The most comm...

Resistance to Isoniazid  The most common pattern of resistance is isolated resistance to isoniazid, which can be treated with rifampin, pyrazinamide and ethambutol for 6-9 mont

Preparation of the standard curve for haemoglobin, Preparation of the stand...

Preparation of the standard curve for haemoglobin? Label 6 clean dry rest tubes and label them as S1 -S6 Add 5 ml of Drabkin's solution to test tube numbers S2 - S6

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd