Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Steam treatment Steam treatment of crop residues under pressure has recently opened a new vista for their nutritional improvement especially in the situation when steam availab
How Viruses Multiply? Obligatory parasitism - Outside cells viruses are nonliving, inactive particles but after entering into live cells these multiply fast by replication
Why do we need a family of proteins for cell adhesion (what is the advantage of having a family of proteins?)
Effect on Microbial Growth and Activity Bacteria require higher aw for growth than fungi. Gram-negative bacteria have higher requirements than gram positives. Most spoilage ba
The stepped steel shaft illustrated in Figure is subjected to a torque (T) at the free end and a torque (2T) in the opposite direction at the junction of the two sizes.
Acute Myocardial Infarction : Patients with acute non-Q myocardial infarction may need urgent intervention as indicated for cases of unstable angina.
Explain Negative Staining Technique? Here, a stain has a negatively charged chromophore that is repelled by the negative bacterial surface. It results in indirect staining of m
Define Various Methods of Food Processing? In this unit, you studied the various methods of food processing. You learnt that food being most vital for the survival of human bei
NON-PROTEI N AMINO ACIDS Non-protein amino acids are the ones which are not incorporated in proteins but occur freely or in combined state in non-protein substances. Their
Explain the Primary and Secondary air Pollutants a. Primary pollutants -emitted directly Particulate matter ( Sulfur dioxide (SO 2 ) Nitrogen dioxide (NO 2 )
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd