Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

How to decreases the rate limiting, When the product of a metabolic pathway...

When the product of a metabolic pathway decreases the rate limiting earlier in the pathway it is called?

State tantalum and niobium in alloplastic implant material, Tantalum and Ni...

Tantalum and Niobium in alloplastic implant materials  Tantalum has been used successfully in needle type, screw- type and double-bladed implants. However, both are difficult

Techniques of plant tissue culture, Techniques of Plant Tissue Culture ...

Techniques of Plant Tissue Culture A standard tissue culture laboratory should provide facilities for washing and storage of glass ware, preparation and storage of nutrient

Alcohol consumption by diabetes patient, Q. Alcohol consumption by diabetes...

Q. Alcohol consumption by diabetes patient? Intake of alcohol should be limited. It is high in calories, lacks essential nutrients and may therefore promote ketoacidosis, hyper

Bovine spongiform encephalopathy (bse), Normal 0 false fal...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Carbohydrates, Carbohydrates Fifty five to seventy per cent of the re...

Carbohydrates Fifty five to seventy per cent of the required energy in animals is derived from carbohydrates. However, fats and proteins can also be broken down and used for

Explain feeding schedule in infancy, Explain Feeding Schedule in Infancy? ...

Explain Feeding Schedule in Infancy? 1) Milk feed 5-6 times/day. Continue breast-feeding. Exclusive breast-feeding up to 6 months even during illness. Follow demand feeding.

To study the structure of a seed, To study the structure of a seed Soak...

To study the structure of a seed Soak seeds of bean, pea, pumpkin, sunflower, corn and other large forms. Take away the seed- coats and carefully cut the seeds open. Discover t

Excretion in earthworm, EXCRETIO N IN EARTHWORM - Main organs are neph...

EXCRETIO N IN EARTHWORM - Main organs are nephredia, pharyngeal, integumentary & septed nephredia. Main are septal nephredia situated on septa.Nephrostome present. Body

+illustrate sturdy evolutionary hypothesis, Q. Why is it a sturdy evolution...

Q. Why is it a sturdy evolutionary hypothesis that although viruses are the structurally simplest beings they were not the first living beings? The fact that viruses are obliga

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd