Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Explain potassium, Explain Potassium Potassium (K): Potassium restri...

Explain Potassium Potassium (K): Potassium restricted diets are important for patients with  advanced renal  failure undergoing  conservative  treatment or haemodialysis. Th

Evaluation of the programme or agency, Evaluation of the programme or agenc...

Evaluation of the programme or agency: It can be  based on following:  i)   Audit or review of programme or agency: This can be continuously done by the staff through in

Determine the organs of the digestive system of earthworms, Which are the c...

Which are the characteristics and organs of the digestive system of earthworms related to the type of diet of these animals? Earthworms eat decomposing organic material and sma

Zoology, give detail account of modes of locomotion in protozoa and describ...

give detail account of modes of locomotion in protozoa and describe various type of asexual reproduction in protozoa

Why all specialization occurred during this time period, There is a lot of ...

There is a lot of dataset and hypothese that attempt to explain why there was a Cambrian Explosion. Using all supporting ideas, explain and write a paragraph on why all this specia

Risk analysis - animal studies, Animal studies Most toxicological data ...

Animal studies Most toxicological data for risk assessment are derived from the animal studies and  it  is, therefore, essential that these studies be conducted following widel

Cardiomyocytes and motor neurons, Describe the mechanisms and genetic regul...

Describe the mechanisms and genetic regulations that underpin how two cells such as cardiomyocyte and a motor neuron have identical gnomes, yet are functionally and structurally di

Vitamins requirement in dyslipidemia, Q. Vitamins requirement in dyslipidem...

Q. Vitamins requirement in dyslipidemia? Antioxidants and flavonoids, natural vitamin E, vitamins C and Aare nutrients (vitamins) that scavenge cell-damaging free radicals and

Find the equilibrium molality of solute species, Find the equilibrium molal...

Find the equilibrium molality of solute species? Consider a hypothetical system in which two aqueous solutions are separated by a semipermeable membrane. Solution α is prepared

What happens within the cell in the second interface period, What are the e...

What are the events that mark the beginning and the end of the second interphase period? What happens in the cell in this period? The second interphase period is the S. It begi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd