Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Mrs. TT seventy three-year old has a long history of chronic obstructive pulmonary disease (COPD). She came to the medical ward as shortness of breath, wheezing and a cough for the
Q. Define Eye Balling? Ans. Two-dimensional echocardiography provides a good visual perception of cardiac functions. With experience, the echocardiologist learns to percei
Erythrocytic cycle The cycle that takes place in the body of man who is considered as secondary host is called the erythrocytic cycle. The first stage of the cycle begins
detail about phylum protozoa?
A tumbler garden Grow several kinds of seeds in 'tumbler gardens'. Every pupil might grow a tumbler garden of his own and keep a day by day pictorial record of the progress of
Explain briefly about the Nutrition? This unit covers the scope of advanced nutrition. It begins with some definitions and basic concepts and briefly traces the history of nutr
Neuston - Aquatic Ecosystem These are unattached organisms which live at the air-water interface such as floating plants and several types of animals. Some spend most of their
WHAT ARE STAKEHOLDERS?
Management of waste amalgam and wastes containing mercury: As all of us know that amalgam is used for restorations very widely.An operator of a dental operation that produces w
Q. What happens to the TSH (thyroid-stimulating hormone) blood level in hypothyroidism? Why is there enlargement of the thyroid in the endemic goiter disease? When there is low
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd