Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Hazard identification We start the process of risk assessment by first identifying the hazard. The goal of hazard identification is to identification potential adverse hea
Q. Pathology of mitral regurgitation? During left ventricular systole as the pressure rises in left ventricle, blood is pumped simultaneously both into aorta and left atrium. T
Measurement of Functional Property of fats and oil Fats and oils have several functional properties that affect the quality of processed foods. In deep fat frying, the roles p
You have discovered a novel alternatively spliced gene (gene X) and you wish to study its regulation in an in vitro splicing assay. The alternatively spliced product retains intro
Determine the Role as antioxidant? Some carotenoids, in addition to serving as a source of vitamin A, have been shown lo function as antioxidants. Studies show that lycopene. (
ARTIFICIA L KIDNEY - Dialysis employs the process of diffusion across a semipermeable membrane to remove unwanted urea . Dialysis is of 2 types - 1. Haemodialysis -
1 ." Most of the genome does not code for protein, therefore 99% of the human genome is junk that serves no purpose". With specific reference to examples long and short non-coding
Determine the types of neuropsychology The practise in neuropsychology is roughly divided into two approaches. Some practitioners use standard comprehensive neuropsychological
Q. Dietary tips for hyperlipideinic patients? It is important to note here that the dietary modifications need to be individual in each patient and these should be tailored lo
Enumerate the term - brain behaviour functioning The brain may be thought of as a "dependant variable" that is shaped in part by the facilitative stimulation that is experience
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd