Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Shoot Multiplication -Clonal Propagation
This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and, therefore, the most popular method of shoot multiplication is forced proliferation of axillary shoots. For this, cultures are initiated from apical or nodal cuttings carrying one or more vegetative buds. In the presence of a cytokinin alone or in combination with a low concentration of an auxin, such as IAA or NAA, the pre-existing buds grow and produce 4-6 shoots (sometimes up to 30-40 shoots) within 3-4 weeks. By periodic removal of individual shoots and planting them on fresh medium of the original composition, the shoot multiplication cycle can be repeated almost indefinitely, and a stock of large number of shoots built up in a short period of time.
Treatments with PGRs as described above can also help in a rapid buildup of shoots by inducing adventitious buds by the explant directly or after callusing. Somatic embryogenesis, which generally occurs after callusing of the explant, is another method of micro propagation. Somatic embryogenesis is not only fast, but may also allow partial automation of micro propagation and the propagules so produced (somatic embryos) bear both, shoot and root meristems. However, adventitive differentiation of shoots or somatic embryos, especially from callus tissue, has the risk of genetic variability in the progeny. Such variation, that develops in tissue culture called "somaclonal variation" is not desirable for micro propagation but is being exploited as a novel source of useful variations for crop improvement.
BASICS OF ENVIRONMENT We live in two world one is natural world of animals, plants, air, water and soil that was present since the evolution of earth. The other world is of soc
Excretion in Animals Excretion is concerned along with the removal of metabolic wastes that arise as a result of oxidation of energy rich compounds and metabolism of proteins
Explain the Reinforcement of Patch using Fibrin Glue? Reinforcement of Patch using Fibrin Glue : In this technique Dacron graft is first sutured to the septum beyond i
Explain the Management of Furcal Perforation 1 st isolate the perforation site. If the perforation is mechanical accidentally occurred: a- It is probably
Explain Cytological Approach in Taxonomy Cytotaxonomy deals with all the aspects of taxonomy at a cellular level. This includes, the structural, genetic and biochemical aspect
Vital Signs of clinical evaluation Patient's clinical evaluation starts with the examination of certain vital signs which are: B.P. (Take average of two regarding with atlea
Protozoans - Regeneration in Invertebrates Most single celled animals such as protists, i.e. protozoans, regenerate very well. If part of the cytoplasm is removed from the
Why Dietary supplements do not speed up a child's growth? Dietary supplements do not "speed up" a child's growth and development: There is no scientific evidence that me
Is the epithelium vascularized? How do nutrients and oxygen reach the epithelium? Why is this feature a significant evolutionary acquisition? Epithelia are not vascularized (ca
will argon tend to form bonds with other elements?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd