Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Shoot Multiplication -Clonal Propagation
This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and, therefore, the most popular method of shoot multiplication is forced proliferation of axillary shoots. For this, cultures are initiated from apical or nodal cuttings carrying one or more vegetative buds. In the presence of a cytokinin alone or in combination with a low concentration of an auxin, such as IAA or NAA, the pre-existing buds grow and produce 4-6 shoots (sometimes up to 30-40 shoots) within 3-4 weeks. By periodic removal of individual shoots and planting them on fresh medium of the original composition, the shoot multiplication cycle can be repeated almost indefinitely, and a stock of large number of shoots built up in a short period of time.
Treatments with PGRs as described above can also help in a rapid buildup of shoots by inducing adventitious buds by the explant directly or after callusing. Somatic embryogenesis, which generally occurs after callusing of the explant, is another method of micro propagation. Somatic embryogenesis is not only fast, but may also allow partial automation of micro propagation and the propagules so produced (somatic embryos) bear both, shoot and root meristems. However, adventitive differentiation of shoots or somatic embryos, especially from callus tissue, has the risk of genetic variability in the progeny. Such variation, that develops in tissue culture called "somaclonal variation" is not desirable for micro propagation but is being exploited as a novel source of useful variations for crop improvement.
Question 1: Describe the need for Harmonization in clinical trials? Brief on the Revised ICH terms of reference and explain the structure of ICH? Need for Harmonization i
what diseases are caused by trypanosoma and entameoba histolytica
EXTERNA L EAR - It is an oval shaped, some what funnel like, skin covered flap of elastic cartilage and muscles. It's outer stiff ridge is called hellix. Lower flexibl
Explain Sickle cell anemia If 4% of a people are born with a severe form of sickle cell anemia. what percent of population will be resistant to malaria in spite of carrying
Explain biological aspects of nutrition Traditionally nutritionists have focused largely (or almost fully) on . However, we have realized over the years, that physiological bio
What is the Importance of vitamin b2 As a prosthetic group of the flavin enzymes, vitamin B 2 is involved in the reactions of almost all nutrients of plants and animals. They
flowers formation without any leaves?
Does every amino acid have a central carbon? To which organic group is that central carbon bound? A carboxyl group -COOH, an amine group - NH 2 , an atom of hydrogen -H and a v
Gastrointestinal Antibiotic prophylaxis is recommended for esophageal surgery in the presence of obstruction, which increases the risk of infection. After gas troduodenal surge
what are phototrophs
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd