Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Shoot Multiplication -Clonal Propagation
This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and, therefore, the most popular method of shoot multiplication is forced proliferation of axillary shoots. For this, cultures are initiated from apical or nodal cuttings carrying one or more vegetative buds. In the presence of a cytokinin alone or in combination with a low concentration of an auxin, such as IAA or NAA, the pre-existing buds grow and produce 4-6 shoots (sometimes up to 30-40 shoots) within 3-4 weeks. By periodic removal of individual shoots and planting them on fresh medium of the original composition, the shoot multiplication cycle can be repeated almost indefinitely, and a stock of large number of shoots built up in a short period of time.
Treatments with PGRs as described above can also help in a rapid buildup of shoots by inducing adventitious buds by the explant directly or after callusing. Somatic embryogenesis, which generally occurs after callusing of the explant, is another method of micro propagation. Somatic embryogenesis is not only fast, but may also allow partial automation of micro propagation and the propagules so produced (somatic embryos) bear both, shoot and root meristems. However, adventitive differentiation of shoots or somatic embryos, especially from callus tissue, has the risk of genetic variability in the progeny. Such variation, that develops in tissue culture called "somaclonal variation" is not desirable for micro propagation but is being exploited as a novel source of useful variations for crop improvement.
Surface Run-off - Causes of Water Pollution The surface run-off from cultivated lands where inorganic fertilisers, pesticides, insecticides and rich manure are applied and sol
What is the substance that stimulates the production of red blood cells? Which is the organ that secretes it? Under what conditions does this secretion increase? The substance
what is binomial nomenclature? What are the importance of it?
Name the disorder in which the immune mechanism of the body of a patient gets suppressed. Which pathogen is responsible for it? Give any two reasons of its transmission.
The peppered moth experiment was an example of directional selection; due to environmental conditions the directional selection shifted from one extreme to the other in 1850s Engla
What is a species? The species is a set of living beings able to cross among themselves generating fertile offspring. This concept though does not apply to individuals of ex
Biological fixation - Nitrogen Fixation Approximately 63% of all nitrogen fixed is through biological fixation. Nitrogen fixing organisms are primarily prokaryotes; bacteria a
Determine Functions of Zinc in the Body? Zinc is an essential component of a large number of enzymes participating in the synthesis and degradation of carbohydrates, lipids, pr
Among neonates, IE typically involves the tricuspid valve of structurally normal hearts and is associated with very high mortality rates. It is likely that many of these episodes
PHOSPHOLIPIDS Most abundant lipid present in cell membrane, also called membrane lipid. It is made up of lipid & phosphoric acid. The basic phospholipid is phosphat
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd