Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Shoot Multiplication -Clonal Propagation
This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and, therefore, the most popular method of shoot multiplication is forced proliferation of axillary shoots. For this, cultures are initiated from apical or nodal cuttings carrying one or more vegetative buds. In the presence of a cytokinin alone or in combination with a low concentration of an auxin, such as IAA or NAA, the pre-existing buds grow and produce 4-6 shoots (sometimes up to 30-40 shoots) within 3-4 weeks. By periodic removal of individual shoots and planting them on fresh medium of the original composition, the shoot multiplication cycle can be repeated almost indefinitely, and a stock of large number of shoots built up in a short period of time.
Treatments with PGRs as described above can also help in a rapid buildup of shoots by inducing adventitious buds by the explant directly or after callusing. Somatic embryogenesis, which generally occurs after callusing of the explant, is another method of micro propagation. Somatic embryogenesis is not only fast, but may also allow partial automation of micro propagation and the propagules so produced (somatic embryos) bear both, shoot and root meristems. However, adventitive differentiation of shoots or somatic embryos, especially from callus tissue, has the risk of genetic variability in the progeny. Such variation, that develops in tissue culture called "somaclonal variation" is not desirable for micro propagation but is being exploited as a novel source of useful variations for crop improvement.
Explain Micafungin It is an echinocandin indicated for treatment of esophageal candidiasis and prophylaxis of invasive Candida infections in patients undergoing hematopoietic s
Duck plague Duck plague, caused by a member of Herpesviridae, has world wide distribution and occurs among domestic and wild ducks, geese, swans and waterfowls. Epidemiolo
Q. How does aldosterone act and where is it produced? Aldosterone is a hormone that acts upon the nephron tubules stimulating the resorption of sodium Therefore it contributes
Disease Control and Prevention reports: Body Mass Index is a reliable indicator of body fat for most children and teens. BMI is calculated from a child's weight and height is
To "express" a gene is to make it to function properly. A gene which encodes a protein will, when expressed, be transcribed or translated to produce that particular protein. A gene
Providing Critical Resources - Conservation of Wildlife Another way to improve the habitat of a threatened species is to determine which resource is limiting the population si
Starr-Edward (S-E) Silastic Ball Valve Prosthesis : This was introduced in 1961 by Albert Stm and has different models for mitral and aortic positions. It is a cage and b
Nursing Interventions Primary goals in acute rheumatic fever are: Control and eradication of the infecting organism. Prevent cardiac complications. Relieve
cours
Use of biotechnology in characterization of animal biodiversity. Genetic uniqueness of populations is measured by the relative genetic distances of such populations from each o
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd