Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Shoot Multiplication -Clonal Propagation
This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and, therefore, the most popular method of shoot multiplication is forced proliferation of axillary shoots. For this, cultures are initiated from apical or nodal cuttings carrying one or more vegetative buds. In the presence of a cytokinin alone or in combination with a low concentration of an auxin, such as IAA or NAA, the pre-existing buds grow and produce 4-6 shoots (sometimes up to 30-40 shoots) within 3-4 weeks. By periodic removal of individual shoots and planting them on fresh medium of the original composition, the shoot multiplication cycle can be repeated almost indefinitely, and a stock of large number of shoots built up in a short period of time.
Treatments with PGRs as described above can also help in a rapid buildup of shoots by inducing adventitious buds by the explant directly or after callusing. Somatic embryogenesis, which generally occurs after callusing of the explant, is another method of micro propagation. Somatic embryogenesis is not only fast, but may also allow partial automation of micro propagation and the propagules so produced (somatic embryos) bear both, shoot and root meristems. However, adventitive differentiation of shoots or somatic embryos, especially from callus tissue, has the risk of genetic variability in the progeny. Such variation, that develops in tissue culture called "somaclonal variation" is not desirable for micro propagation but is being exploited as a novel source of useful variations for crop improvement.
Importance of Dark Period For quite sometime the role of light period (photoperiod) was emphasised in flowering? However, based on certain experiments it was realised that it
Q. What are some diseases caused by abnormal GH secretion by the hypophysis? In childhood deficient GH secretion may lead to delayed growth and in severe cases to nanism that i
What are the subkingdoms into which the plant kingdom is divided? The kingdom Plantae is separated into two big subkingdoms: the bryophytes and the tracheophytes (pteridophytes
CARE OF RECORDS Patient's/client's records are unquestionably a valuable documents. These should be made on paper of good quality and protected from soiling, and burning. Th
Q. Why can the amnion also be considered an adaptation to terrestrial life? The amnion is as well an adaptation to dry land since one of its functions is to prevent desiccation
Major Criteria 1) Positive blood culture • Typical microorganism for infective endocarditis from two separate blood cultures Viridans streptococci, Streptococcus bovis, HAC
Proteins of Plant Origin - Nuts Nuts are excellent sources of proteins. Examples of nuts include cashew nuts, almond nuts , hazel nuts , coconuts, walnuts, brazil nuts ,cashew
What are the Luria's methods and theories Adhering to our theory that the Luria Nebraska bears little resemblance to Luria's methods and theories, there seems little point in i
Explain Vasopressindrug term? As a single bolus of 40 U, vasopressin has been recommended as an alternative to epinephrine in VF/pulseless VT refractory to defibrillation. Furt
Q. What are respiratory pigments? What are some respiratory pigments and in which animal groups can each of them be found? Respiratory pigments are molecules present in the blo
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd