Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Explain about cesium-chloride gradients, Matthew Meselson and Frank Stahl g...

Matthew Meselson and Frank Stahl grew bacteria in media having either N14 or N15 for various numbers of cell divisions. DNA from the bacterial samples were isolated and spun within

Why does thymine replace uracil in dna, Q. Why does thymine replace uracil ...

Q. Why does thymine replace uracil in DNA? The difference between DNA and RNA is existence of a hydroxyl (-OH) group on 2' carbon of the ribose sugar in the backbone. Removal o

Define the cooper''s run (12 minute run) method, Define the Cooper's Run (1...

Define the Cooper's Run (12 minute run) Method? To undertake this test, a 400 metre running track is required-marked every 100 m and a stop watch. The test comprises of noticin

Explain the neuritic type of infantile beriberi, Explain the neuritic type ...

Explain the neuritic type of infantile beriberi? It is also referred to as Wernicke korsakoff syndrome or cerebral beriberi. It shows typical manifestations of peripheral neuro

Anti platelet drugs-peri operative problems, Anti Platelet Drugs In t...

Anti Platelet Drugs In the early years of CABG, patients used to be put on aspirin and persantin (Dipyridamole). Persantin is not routinely prescribed now. Aspirin dose can b

What are the chemical bonds of the dna molecule, Q. What are the chemical b...

Q. What are the chemical bonds of the DNA molecule that are broken for replication process to occur? During the DNA replication process, hydrogen bonds between the nitrogen- co

Loading and unloading of sieve tubes, Loading and Unloading of Sieve Tubes ...

Loading and Unloading of Sieve Tubes In order to understand the loading of food from manufacturing leaf cells to sieve tubes we must examine the anatomy of a minor vein shown

Biostatistics for clinical and public health research, Disease Y Study: Le...

Disease Y Study: Let us consider the Disease Y study, a brief description of the study is as follows. A case-control study was conducted of 75 cases with a rare genetic disorder "

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd