Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Eexplain importance of mitosis for the embryonic development, What is the i...

What is the importance of mitosis for the embryonic development? Every embryo grows from a single cell that suffers mitosis and operates other cells that also divide themselves

What is cytosolic cyclic amp, What is cytosolic cyclic AMP Healthy Pers...

What is cytosolic cyclic AMP Healthy Person P takes a new drug that is a member of a drug family that results in  constant levels of cytosolic cyclic AMP (cAMP) in one and only

How much proteins should be taken for management of obesity, How much Prote...

How much Protein should be taken for Management of Obesity? Adequate amount of proteins should be included in the diet to ensure proper metabolism and prevent weakness which is

Define measuring body composition- skin fold measurement, Define measuring ...

Define measuring body composition- Skin fold measurement? The clinical use, skin fold measurement can be taken with the help of calipers. The calipers are applied at constant p

Can you explain deoxynivalenol mycotoxicosis, Q. Can you explain Deoxynival...

Q. Can you explain Deoxynivalenol Mycotoxicosis? During 1987, an outbreak estimated to have affected over 50,000 people in the State of Jammu and Kashmir, was traced to the c

Describe the common operations performed in pcs, Describe the common operat...

Describe the common operations performed in paediatric cardiac surgery 1) Closed Heart Surgery: Surgery on Heart or Great Vessels done without using the Heart-Lung machine.

False positive st changes, Q. False Positive ST Changes? 1) The slope ...

Q. False Positive ST Changes? 1) The slope of the PQ-segment can help predict the magnitude of the influence of P-wave depolarization and thus help predict which patient wou

What are the antigens, What are the antigens and the respective antibodies ...

What are the antigens and the respective antibodies of the ABO blood group system? The ABO blood system contains the erythrocytic antigens A and B that can be attacked by the a

Dietary considerations oesophagitis, Q. Dietary considerations oesophagitis...

Q. Dietary considerations oesophagitis? The dietary considerations for this condition are the same as in oesophag it is. The patient should be recommended to: • eat smaller

Define role of vitamin d in controlling gene expression, Define role of Vit...

Define role of Vitamin D in controlling gene expression? Vitamin D is a secosteroid that is metabolically activated and degraded through the actions of three cytochrome P450 hy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd