Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

gas exchange, what are the organs of respiration in the lower form of anim...

what are the organs of respiration in the lower form of animals?

Explain the malthus theory about population growth, Explain the Malthus' Th...

Explain the Malthus' Theory about population growth? Darwin's Theory was also based on Thomas Malthus' writings on population growth, in which he explained that populations gro

What would be the progeny of these crosses, Suppose that you are raising Do...

Suppose that you are raising Downton wombats, carefully because they bite when teased.You notice that some of your wombats have white spots, whereas others have solid coats. What t

What are enzyme cofactors, What are enzyme cofactors? Some enzymes requ...

What are enzyme cofactors? Some enzymes require other associated molecules to work. These molecules are known as enzyme cofactors and they can be, for example, organic ions lik

Advise on rest, Advise on Rest, recreation, sleep The patient should t...

Advise on Rest, recreation, sleep The patient should take enough rest and get good sleep. Restrict the visitors so that the patient can get enough rest. Not to strain th

Define evolution of food processing, Define Evolution of food processing? ...

Define Evolution of food processing? 8000 - 7000 BC. Mankind first began farming, growing crops and raising animals for food instead of hunting and gathering for food.

Explain the term light reflex - neuronal pathways, Explain the term Light r...

Explain the term Light reflex - Neuronal pathways The neuronal pathway for light reflex can be afferent and efferent. In the afferent pathway, the pupillary fibres begin in the

Epimorphic regeneration, Epimorphic Regeneration In this sort of regen...

Epimorphic Regeneration In this sort of regeneration the lost part is reformed and restored via the growth of a bud or blastema from the remaining part of the organism followe

Original density in CFU/ML, a nutrient agar plate labelled 10^(-5)ml had 15...

a nutrient agar plate labelled 10^(-5)ml had 154 colonies after incubated. what is the cell density in the original sample

Cyanotic congenital heart disease, Cyanotic Congenital Heart Disease   ...

Cyanotic Congenital Heart Disease   So far we have discussed the acyanotic congenital heart disease. Now we shall discuss some of  the main cyanotic congenital heart diseases.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd