Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Typological species concept, The typological species concept was suggested ...

The typological species concept was suggested by Plato more than 2000 years ago. According to this concept, the immense variety in nature can be reduced to a few "types". Individua

Toxoplasma cycles, Toxoplasma cycles between its rodent and feline hosts,li...

Toxoplasma cycles between its rodent and feline hosts,living out different phases of its existence in each. what is the scientific means about the cat and mouse.

Diversty of living organisms, write down the division of cryptogamae and p...

write down the division of cryptogamae and phanerogamae

Importance of dark period, Importance of Dark Period For quite sometim...

Importance of Dark Period For quite sometime the role of light period (photoperiod) was emphasised in flowering? However, based on certain experiments it was realised that it

Exon, Exon are those parts of a genomic DNA sequence which will be represe...

Exon are those parts of a genomic DNA sequence which will be represented in final, mature mRNA t A contiguous segment of the genomic DNA which codes for a polypeptide in the gene.

Mode of hormone action through extracellular receptors, MOD E OF HORMONE A...

MOD E OF HORMONE ACTION THROUGH EXTRACELLULAR RECEPTORS - The molecules of amino acid derivatives, peptides or polypeptide (protein) hormones bind to specific receptor molecul

Ribosome in the translation of one mrna molecule, Q. How many of the same p...

Q. How many of the same proteins are made at the same time by each ribosome in the translation of one mRNA molecule? How does successive protein production occur in translation?

Define national programmes targeting children and adolescent, Define Nation...

Define National Programmes Targeting Children and Adolescents? Two programmes specially target children and adolescents as a vulnerable group. These are: a) Mid Day Meal Pro

Explain precautions for performing completed coliform test, Explain Precaut...

Explain Precautions for Performing the Completed Coliform Test 1. Aseptic conditions should be adopted while performing the experiment. 2. Destaining is the critical step in

Implantation, Implantation : It is the process in which the blastocyst (de...

Implantation : It is the process in which the blastocyst (developing zygote) is attached to the glandular uterine wall and then embedded in the wall of uterus. 1) In human bein

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd