Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Define some example of acidic and basic buffer, Define Some Example of Acid...

Define Some Example of Acidic and Basic Buffer? Frequently used example of acidic and basic buffer includes: - Acidic buffer: a mixture of CH 3 COOH (acetic acid) + CH 3 C

Agro industrial-oil seed meals as by-pass protein source, Oil Seed Meals as...

Oil Seed Meals as By-pass Protein Source The protein digestion in ruminants encompasses microbial transformation of dietary protein in the rumin. A varying portion of feed pro

What is fern allies - equisetum, What is Fern Allies - Equisetum ? The ...

What is Fern Allies - Equisetum ? The earliest land plants are thought to have evolved from green algal ancestors, since they share many features. Both green algae and plants h

Difference between eye and camera, Difference between Eye and Camera - ...

Difference between Eye and Camera -   Camera   Ey e 1. Box.   2. Black inner paint   3. Shutter   4. Diaph

Define excretion of methyl malonic acid in urine, Define Excretion of Methy...

Define Excretion of Methyl malonic acid in urine (Methyl malonil aciduria)? Methyl malonic acid is found only in traces in normal urine (1-2 mg/day). In vitamin  B 12 deficie

Explain process of glycosylation, Glycosylation: The covalent addition of ...

Glycosylation: The covalent addition of the sugar moities to N or O atoms present in the side chains of various amino acids of certain proteins, usually occuring within the Golgi

How are water and mineral salts tranport in the plant, How are water, miner...

How are water, mineral salts and food (sugar) transported throughout the plant? Water, mineral salts and sugar are transported throughout the plant through conductive vessels f

Managing diarrhoea in children, Q. Managing diarrhoea in children? Mana...

Q. Managing diarrhoea in children? Managing diarrhoea in children is of great concern. The guidelines for the same are as follows: 1. The first objective is to rehydrate the

What is chemical disinfection, Q. What is Chemical disinfection? Chemic...

Q. What is Chemical disinfection? Chemicals are added to waste to kill or inactivate the pathogens it contains, this treatment usually results in disinfection rather than steri

Explain the nervous system characterized in echinoderms, How are the symmet...

How are the symmetry and the nervous system characterized in echinoderms? Adult echinoderms, with cnidarians, are the animals that present radial symmetry, i.e., their body str

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd