Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

What are the examples of secretory cells, Q. What are the examples of secre...

Q. What are the examples of secretory cells? Endocrine and exocrine pancreatic cells, parathyroid and thyroid endocrine cells, adenohypophysis, adrenal and pineal endocrine cel

List five advantages of dsme, Q. List five advantages of DSME? Followin...

Q. List five advantages of DSME? Following are the advantages of DSME. It enables the patient to 1. Accept the disease. 2. Gain knowledge about disease, its prevention

Transcription and processing of trna in eukaryotes, In the eukaryotes, the ...

In the eukaryotes, the transmit RNA genes exist as multiple copies and are transcribed by RNA polymerase III or RNA Pol III. As in prokaryotes, various tRNAs may be transcribed tog

What are some things that have algae in them, What are some things that hav...

What are some things that have algae in them? Yeast is considered Algae.

Vertibrates, what is the classification of vertibrates

what is the classification of vertibrates

Define vitamins b12 deficiency in vegans, Define Vitamins B 12 Deficiency ...

Define Vitamins B 12 Deficiency in Vegans? Because plants do not synthesize vitamin B 12 , individuals who consume diets completely free of animal products (vegan diets) are a

Explain the effect on vitamins of the human milk, Explain the effect on Vit...

Explain the effect on Vitamins of the human milk? Although there are inter-individual variations in vitamin concentrations, diet and drug use by individual women influences vit

Unilateral incompatibility, Unilateral Incompatibility This relates to...

Unilateral Incompatibility This relates to incompatibility that operates in one direction. The most common way to ascertain unilateral incompatibility is to perform crosses be

Vaccines for gas infections, In developing countries because of poor compli...

In developing countries because of poor compliance, poverty, ignorance, primary and secondary prevention strategies are difficult to implement properly. Also in western countries (

Explain the interaction of riboflavin with other nutrients, Explain the Int...

Explain the Interaction of Riboflavin with other Nutrients? Riboflavin, as already discussed under the functions, interrelates with other B vitamins,  notably niacin, which r

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd