Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

What are the five dna polymerases, The Eukaryotic cells have five several D...

The Eukaryotic cells have five several DNA polymerases; α, β, γ, δ or ε. The DNA polymerases contained in replication of chromosomal DNA are α and δ. DNA polymerases β or ε are con

Basic physical concepts of plant water relation, Basic Physical Concepts of...

Basic Physical Concepts of Plant Water Relation The main idea of this section is to explain the concept of chemical potential of water or water potential and the effect of var

Explain highly active antiretroviral therapy, Highly active antiretroviral ...

Highly active antiretroviral therapy (HAART) - The treatment of HIV infection requires combination therapy known as highly active antiretroviral therapy (HAART) along with inte

Care of records, CARE OF RECORDS Patient's/client's records are unque...

CARE OF RECORDS Patient's/client's records are unquestionably a valuable documents. These should be made on paper of good quality and protected from soiling, and burning. Th

What is density-dependent and density-independent factors, What is Density-...

What is Density-dependent and density-independent Factors? Density-dependent : Ecologists identify population-regulating mechanisms whose functioning is related to density a

Orthopedics, 1. List alternative therapies for musculoskeletal problems. ...

1. List alternative therapies for musculoskeletal problems. 2. What is a xyrospasm? What is the Greek derivative of the word? 3. Name and describe the three types of varus con

Show the peptide found in the brain, Q. Show the Peptide found in the brain...

Q. Show the Peptide found in the brain? There are as many as 300 peptide neurotransmitters found in the brain. Peptide is a short protein consisting of fewer than 100 amino aci

Hypothermia-open heart surgery, Hypothermia :  Hypothermia reduces the met...

Hypothermia :  Hypothermia reduces the metabolic requirements of the body thereby reducing oxygen consumption. It also preserves high-energy phosphate stores of the body. At norma

How plants classify to photoperiodism-based flowring, How do the plants cla...

How do the plants classify according to their photoperiodism-based flowering? According to their photoperiodism-based the flowering plants classify as long-day plants, those th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd