Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Define precipitation or solubility of proteins, Define Precipitation or Sol...

Define Precipitation or Solubility of Proteins? Most of the functional properties are dependent on the degree to which the proteins are soluble. The solubility behaviour provid

What is a vesicle, What is a vesicle? A vesicle is a relatively minute ...

What is a vesicle? A vesicle is a relatively minute intracellular, membrane-enclosed sac that keeps or transports substances

What do you mean by cytotaxonomu and biosustematics, Q. What do you mean by...

Q. What do you mean by Cytotaxonomu and biosustematics? Towards the end of the 19 th century and in the early years of the 20th century, botanists were faced with a problem of

Short note on glycogen storage diseases, Write short note on glycogen stora...

Write short note on glycogen storage diseases. Glycogen storage diseases are caused by genetic defects  that  result in deficiencies in certain enzymes of glycogen metabolism.

Clinical presentation and investigation of hypertension, Any elevation in B...

Any elevation in BP is accompanied by increased risk of cardiovascular and cerebrovascular events and renal damage. The most distinctive pathological consequence

Explain the classification of malignant tumors, Explain the Classification ...

Explain the Classification of Malignant Tumors Malignant tumors can be classified as: Carcinomas: These tumors arise from the epithelial lining (the squamous cells) e.g. ski

How do placental mammals reproduce, How do placental mammals reproduce? ...

How do placental mammals reproduce? Placental mammals reproduce sexually, they have internal fecundation and they are viviparous, i.e., their embryo creates within the mother's

How did darwin reach the principle of natural selection, How did Darwin rea...

How did Darwin reach the principle of natural selection from the observation of differences among individuals of the same species? The Darwin recognized that in a same species

Excretion in protozoans, Excretion in Protozoans Excretion of metaboli...

Excretion in Protozoans Excretion of metabolic wastes is dope almost exclusively by diffusion. All protozoans are ammonotelic i.e., the end product of their nitrogen metabolis

What is nagel''s anomaloscope, What is Nagel's Anomaloscope The patient...

What is Nagel's Anomaloscope The patient is asked to match a yellow with a mixture of red and green wave lengths. A defect can be detected based on the amount of red and green

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd