Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Explain the procedure for preparation of bacterial smear, Explain the Proce...

Explain the Procedure for Preparation of Bacterial Smear? Start the exercise by following the steps enumerated herewith. (1) Take properly washed and dried glass slides for

What do you understand by the term accommodation, (a) What do you understan...

(a) What do you understand by the term 'accommodation'? (b) What part does the lens play in this process?   a) Accommodation is the way the eye can focus either

Which are plant tissues that cover the stem and the leaves, Which are plant...

Which are plant tissues that cover the stem and the leaves? The stem perhaps covered by epidermis (having stomata, cuticle and photosynthetic cells) as in monocots or, alternat

Photosynthesis, test to prove that carbondioxide is needed for photosynthes...

test to prove that carbondioxide is needed for photosynthesis to take place

Contractile cells, CONTRACTI L E CELLS In addition to above mentioned...

CONTRACTI L E CELLS In addition to above mentioned three types of muscular tissue, there are contractile cells. These are as follows. 1.      Myoepithelial Cells (= M

Dietary recommendations in celiac disease, Q. Dietary Recommendations in ce...

Q. Dietary Recommendations in celiac disease? The only dietary treatment for celiac disease is to follow a gluten-free diet. For most such a diet improves symptoms, heals inte

nutrient stability as affected by processing of feeds, Nutrient stability ...

Nutrient stability as affected by processing of feeds Nutrient stability under normal conditions of storage and processing is one of the important factors determining the effe

Name the classes of biomaterials, Explain the classes of biomaterials W...

Explain the classes of biomaterials When an artificial material is placed in the human body, tissue reacts in a variety of ways depending on the material type thereby, affectin

Define pre-exercise or pre-event meal for athletes, Define Pre-Exercise or ...

Define Pre-Exercise or Pre-Event Meal for athletes? Suitable foods in adequate quantities at all times should be consumed but before the event, strategies to fuel up the energ

Dimensions of the cells, On a given microscope, the numerical aperture of t...

On a given microscope, the numerical aperture of the objective is 1.5. The microscope is provided with a filter that selects a wavelength of 500 nm. What is the limit of resolution

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd