Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Drawbacks of lamellar model, Drawbacks of Lamellar model This model ...

Drawbacks of Lamellar model This model does not explain the elasticity of PM. Although this model says that PM is elastic but it also states that head of lipid molecules is

Cnidarian, what process are nematocysts formed by the cnidoblast?

what process are nematocysts formed by the cnidoblast?

Explain prophylaxis - diarrhea, Prophylaxis Medical Letter consultants...

Prophylaxis Medical Letter consultants generally do not prescribe antibiotic prophylaxis for travelers' diarrhea, but rather instruct thec patient to begin self-treatment when

Explain linolenic acids, Linolenic Acids Fats in this group contain sub...

Linolenic Acids Fats in this group contain substantial amounts of linolenic acid (C18 triunsaturated). Examples are soybean, mustard, rapeseed, flaxseed and wheat germ hemps

Horse diseases-african horse sickness, African horse sickness African h...

African horse sickness African horse sickness (AHS) is a highly fatal insect born viral disease of equidae caused by an Orbivirus (family Reoviridae) and characterized by sever

Explain the indole production test, Explain the Indole Production Test? ...

Explain the Indole Production Test? Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can

Illustrate in detail about the cell, Illustrate in detail about the Cell ...

Illustrate in detail about the Cell The present day study of cells combines many scientific disciplines, like biochemistry, biophysics, genetics, microscopy and physiology. A g

Cold hardiness and cold acclimation, Cold Hardiness and Cold Acclimation ...

Cold Hardiness and Cold Acclimation In connection with cold response we would like to introduce two terms- cold hardiness and cold acclimation. Cold-hardiness ref

Community, COMMUNITY If you look around yourself you will notice that pop...

COMMUNITY If you look around yourself you will notice that populations of plants and animals seldom occur by themselves. The reason for this is quite obvious. In order to survive

What are intraspecific ecological interactions, What are intraspecific and ...

What are intraspecific and interspecific ecological interactions? Intraspecific ecological interactions are those among individuals of the same species. Interspecific ecologic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd