Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Polarity - root and shoot morphogenesis, Polarity - Root and Shoot Morphoge...

Polarity - Root and Shoot Morphogenesis Polarity is defined as having a difference (morphological, physiological or both) between the two ends of an axis or cells. This is lar

Percentage of reducing and total sugar by fehling soxhlet, Principle for Es...

Principle for Estimation of Percentage of Reducing and Total Sugar by Fehling Soxhlet Method? Reducing sugars are those which have free sugar groups and hence may be estimated

Mitral valve replacement with allograft , Mitral Valve replacement with All...

Mitral Valve replacement with Allograft (Mitral homograft) :  C. Acar and colleagues used mitral allograft for mitral valvc replacement using a new technique of fixation of p

Locomotor organelles, Locomotor Organelles The protozoan locomotor org...

Locomotor Organelles The protozoan locomotor organelle may be Flagella, Cilia or Pseudopodia. These are of considerable value in classification of protozo

Airway management - respiratory failure, Airway Management To improve...

Airway Management To improved ventilation, suctioning, IPPB, Ultrasonic mist therapy and postural drainage with clapping and vibrating are all employed to halt the progress o

Classification, What is omnispective classification

What is omnispective classification

Heredity, in humans, maleness of is determined by a pair of sex chromosomes...

in humans, maleness of is determined by a pair of sex chromosomes called X and Y. (a)what is the genotype for males? (b)what is the genotype for females?

Define briefly about the pyridoxine vitamin, Define Briefly about the Pyrid...

Define Briefly about the Pyridoxine vitamin B 6 ? Pyridoxine or vitamin B 6 is one of the B complex vitamins which prevents and cures dermatitis in rats fed on vitamin B 6 de

Why photoperiodism affect the flowering of some plants, How does the photop...

How does the photoperiodism affect the flowering of some plants? Flowering is a typical and simple to observe instance of photoperiodism. Most flowering plants flower only duri

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd