Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Classification of anaemia based on etiology, Classification of Anaemias Bas...

Classification of Anaemias Based on Etiology   Anaemia  can be classified  as   Impaired Production of Red Blood Cells and Hemoglobin: This can be due to nutritional  def

Excretory system of animals and human urinary system, steps in urine format...

steps in urine formation and the organs/tissues involve in such process?

Transmembrane voltage, How come that difference of K+ and Na+, which are bo...

How come that difference of K+ and Na+, which are both monovalent cations, creates the difference of the net charge of the membrane at different sides?

Determine the process of circulatory system, How does the circulatory syste...

How does the circulatory system participate in the functioning of the endocrine system? The circulatory system is fundamental for the performance of the endocrine system. The b

What is light reaction, What is light reaction? Light reactions are rea...

What is light reaction? Light reactions are reactions, which are initiated by light (also photo-induced reactions). Usually, light energy is change into chemical energy.

Filariasis, Filariasis Filariasis is a chronic infection caused by filarid...

Filariasis Filariasis is a chronic infection caused by filarid worms. Around 10 species of Filaridae family are parasitic to man. Adult worms live in the tissues or body cavities

Zooflagellates – protozoan, Zooflagellates – Protozoan Zooflagellates ...

Zooflagellates – Protozoan Zooflagellates are heterotrophs that are free living, commensals or symbiotic or parasitic in other animals. A number of species eg. Trichonynzpha a

Role of water in metabolism, ROL E OF WATER - In general water cons...

ROL E OF WATER - In general water constitutes 60-90% of protoplasm. Average 80%. It forms 2/3 of human body weight, i.e. 66.66%. Due to electrophillic nature of O, wa

Illustrate steps of polymerase chain reaction , There are three steps of PC...

There are three steps of PCR a) Denaturation.  The reaction combination is heated to 95°C for a short time period (about 15-30 sec) to denature the goal DNA into one strands w

Explain highly active antiretroviral therapy, Highly active antiretroviral ...

Highly active antiretroviral therapy (HAART) - The treatment of HIV infection requires combination therapy known as highly active antiretroviral therapy (HAART) along with inte

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd