Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Biological responses controlled by phytochrome, Biological Responses Contro...

Biological Responses Controlled by Phytochrome Phytochrome responses are those which are controlled reversibly by red and far-red light. These can be broadly categorised as:

Sodium - mineral elements, SODIUM The element is available in table sa...

SODIUM The element is available in table salt. A proper balance of sodium and potassium is essential. Its absorption is under control of aldasterone. The various f

Determine the term - neuropsychology, Determine the term - Neuropsychology ...

Determine the term - Neuropsychology The neuropsychology of the various modalities and domains involves applications from the knowledge base concerning the domain or modality i

Explain the cladosporium - fungi and yeast, Explain the Cladosporium - Fung...

Explain the Cladosporium - Fungi and Yeast? Classification Kingdom - Mycetae Division - Amastigomycota Form - Class - Deauteromycetes Form - Order - Moniliales F

Cotyledon, Cotyledon is a leaf-like structure which is present in the seed...

Cotyledon is a leaf-like structure which is present in the seeds of the flowering plants; it appears during seed germination and sometimes is referred to as the seed leaf.

Define the fact that we must eat to gain the energy, Which law of thermodyn...

Which law of thermodynamics explains the fact that we must eat to gain the energy to perform the functions of life, such as breathing? a. fourth law b. first law c. second law d

Nursing care plan, Mrs. TT seventy three-year old has a long history of chr...

Mrs. TT seventy three-year old has a long history of chronic obstructive pulmonary disease (COPD). She came to the medical ward as shortness of breath, wheezing and a cough for the

Provide evidence to explain the conversion, Provide supporting evidence to ...

Provide supporting evidence to explain how conversion of a normal somatic cell into a malignant one requires multiple mutations.

How does the presence of exoskeleton, Q How does the presence of exoskeleto...

Q How does the presence of exoskeleton describe the general small size of arthropods? Since they have periodic ecdysis and exoskeleton, the growth of arthropods is limited to a

Chances of change in the gene pool, A genetic trait which is considered to ...

A genetic trait which is considered to be harmful in the present day might prove to be beneficial in future. For example by removing the trait for sickle cell we may increase the s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd