Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

How sugar is used in bakery application, How Sugar is used in Bakery Applic...

How Sugar is used in Bakery Application? In bakery applications, sugars are used to impart flavor, aroma and color. During the mixing process, excess gluten development can mak

Fishes- regeneration in vertebrates, Fishes- Regeneration in Vertebrates ...

Fishes- Regeneration in Vertebrates Several different parts of the fish body will re-grow. Plucked scales are promptly replaced by new ones and amputated gill filaments can re

Pollen stigma interaction - stigma, Pollen Stigma Interaction - Stigma ...

Pollen Stigma Interaction - Stigma The Stigma After landing on the stigma pollen grain germinates, and produces a pollen tube that carries the male gametes. The stigma has bee

Define the goals of treatment of obesity, Define the Goals of treatment of ...

Define the Goals of treatment of Obesity? As said in the beginning, the goal of treatment of obesity today has shifted from mere 'weight loss' to 'weight management'. Each weig

Explain requirement for simple staining of bacterial culture, Explain Requi...

Explain Requirements for Simple Staining of Bacterial Cultures Culture: 24 hours bacterial culture of E. coli and Bacillus on nutrient agar or in broth. Reagents: Methylene

Names and uses of the various laboratory tools, What are the names and uses...

What are the names and uses of the various laboratory tools? Tools are beakers, microscopes, tweezers, hot plates, lasers, test tubes, voltmeters, Erlenmeyer flasks, thermomete

What are abiotic factors, What are abiotic factors? Abiotic factors are...

What are abiotic factors? Abiotic factors are the nonliving elements that constitute a given environment, as light, temperature, minerals, gases, water, atmospheric pressure, e

What are allele frequencies, You observe the following numbers of individua...

You observe the following numbers of individuals of each genotype in a population. AA=700, Aa=200, aa=100. What are the observed genotype frequencies in this population? What are t

Economic and ecological importance of molluscs, Q. What are examples of the...

Q. What are examples of the economic and ecological importance of molluscs? Molluscs are important players in several food chains in ecosystems. Many marine molluscs are part o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd