Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Kingdom animalia, in what order did the shellfish belong? in what genus did...

in what order did the shellfish belong? in what genus did the shellfish belong? in what species did the shellfish belong?

Progressive senescence - senescence, Progressive senescence - Senescence ...

Progressive senescence - Senescence This is the third category of senescence. Here also the leaves are shed but it is gradual senescence of leaves up the stem. For example, th

Describe the complete transposition of great arteries, Describe the Complet...

Describe the Complete transposition of great arteries ? Lethal, relatively frequent malformation. Without treatment, it results in 30 per cent mortality within the first week o

Psyiology, I need help with p-p, q-q and r-r intervals and heart rates

I need help with p-p, q-q and r-r intervals and heart rates

What is karyotype, What is karyotype? The name karyotype is given to th...

What is karyotype? The name karyotype is given to the set of chromosomes of an individual, generally when visualized and identified under the microscope. The visualization usua

What is energy, What is Energy ? Energy Energy is defined as the cap...

What is Energy ? Energy Energy is defined as the capacity to do work. Work is defined as the movement of a mass, or the product of the force and the distance through which t

What is the basic role of botanic garden, Q. What is the basic Role of Bota...

Q. What is the basic Role of Botanic Garden? A botanic garden makes us to simply enjoy the beauty and variety of nature. The pleasure of admiring turns us into good naturalists

What is the tree girdling, What is the tree girdling? What happens to the p...

What is the tree girdling? What happens to the plant when that girdle is removed from the stem (below the branches)? Tree girdling or Malpighi's girdling is the removal from a

Explain the cori cycle, The  Cori cycle a)  Pyruvate formed from glucos...

The  Cori cycle a)  Pyruvate formed from glucose is converted  to lactate by lactate dehydrogenase in the muscle cell. b)  Lactate is released into  the blood and taken up b

Advantage and disadvantages of fensom and spanner, Advantage and disadvanta...

Advantage and disadvantages of Fensom and Spanner Electroosmotic Flow Hypothesis The model has several advantages over Munch's model. The presence of P- proteins, and the occl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd