Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Radioactive labelling, Radioactive Labelling Radioactive labelling me...

Radioactive Labelling Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part

Classifications of carbohydrates based on number of carbon, Define the Clas...

Define the Classifications of carbohydrates based on number of carbon?   A chain-form monosaccharide that has a carbonyl group (C= O) on an end carbon forming an aldehy

ANAT 260 Signature Assignment, ANAT 260 Signature Assignment Instructions: ...

ANAT 260 Signature Assignment Instructions: Address each question below as it relates to the case study given. A patient was brought to the Emergency Department by ambulance with t

What do you mean by connective tissue proper, Q. What is connective tissue ...

Q. What is connective tissue proper? The name connective tissue proper is used to designate the connective tissue that fills interstitial spaces as opposed to the specialized c

Dysrhythmias - complications of cardiac surgery, Dysrhythmias Usually ...

Dysrhythmias Usually occur during the initial 24-72 hours, but may occur later on also. The causes may be ventricular irritability due to manipulations of heart durin

Explain the modes of ventilation, Explain the Modes of Ventilation? In ...

Explain the Modes of Ventilation? In general, mechanical ventilation can be done to deliver a set tidal volume (volume controlled) or a set pressure (pressure controlled). Some

Define interaction of folate with vitamin b12, Define interaction of folate...

Define interaction of folate with vitamin B 12 ? Vitamin B 12 : For the conversion of folic acid to folinic acid, vitamin B 12 is required. Vitamin BIZ deficiency caus

The formation of macro and micronuclei of daughter paramoeci, The formation...

The formation of macro and micronuclei of daughter paramoeci: Each exconjugant has a single micronucleus and four macronuclei. This type of exconjugant divides twice and f

Explain about the nucleoproteins, Explain about the Nucleoproteins? Nuc...

Explain about the Nucleoproteins? Nucleoproteins are combinations of nucleic acids land simple proteins, which usually consists of a large number of basic amino acids. Nucleopr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd