Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Where do the two neuronal pathways diverge, Where do the two neuronal pathw...

Where do the two neuronal pathways diverge For both the neuronal pathways i.e. light reflex and near reflex the efferent fibres go to are 3rd cranial nerve and then to sphinct

Drug management for gout disease, Q. Drug Management for gout disease? ...

Q. Drug Management for gout disease? Drugs are so effective in lowering the serum mate concentration that rigid restriction of dietary purines is rarely necessary. Uricosurie d

What is life threatening haemorrhage, What is Life threatening haemorrhage ...

What is Life threatening haemorrhage Life threatening haemorrhage has been reported when a drill perforates the sublingual region of the mandible and injures the sublingual arte

What is genetic mapping, How can the concept of recombination frequency be ...

How can the concept of recombination frequency be used in genetic mapping? Genetic mapping is the determination of the location of the genes in a chromosome. By determining

Gastrulation in amphibians, Gastrulation in Amphibians Amphibians com...

Gastrulation in Amphibians Amphibians comprise a large and moderately telolecithal egg. Cleavage is holoblastic and unequal generating a spherical blastula along with a reduc

What is the typical shape of a population growth curve, Q. What is the typi...

Q. What is the typical shape of a population growth curve? How can the biotic potential be represented in the same way graphically? A usual population growth curve number of in

Indications for surgery-mitral valve disease, Indications for Surgery :  T...

Indications for Surgery :  The normal mitral valve area is 4-5 cm 2 . Usually symptoms appear when the valve area has become less than 2.5 cm2. Symptoms are present at rest when t

Alkaloids, Miscellaneous antinutritional substances Alkaloids: Alkal...

Miscellaneous antinutritional substances Alkaloids: Alkaloids represent a large and structurally diverse group of compounds present within angiosperm plants; the majority of

What are the genotype and phenotype of the unknown male, For Dalmation dog,...

For Dalmation dog, the spotted condition is dominant to non-spotted. a) Using a Punnet square, show a cross between two heterozygous parents. b) A spotted female Dalmation dog is m

Temperate deciduous forest - ecosystem, Temperate deciduous forest - Ecosys...

Temperate deciduous forest - Ecosystem The temperate forests are characterised by a moderate climate and broad-leafed deciduous trees, which shed their leaves in fall, are bar

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd