Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Explain the manifestations of iron deficiency anaemia, Explain the Manifest...

Explain the Manifestations of Iron Deficiency Anaemia? • Paleness of conjunctiva • Paleness of tongue • Paleness of mucosa of soft palate • Low haemoglobin • Swelling of

Respiration, explain the 4 stages of aerobic respiration

explain the 4 stages of aerobic respiration

Nutritional requirements of fats and oils during pregnancy, Define Nutritio...

Define Nutritional Requirements of Fats and Oils during Pregnancy and Lactation? ICMR (1990) recommends that linoleic acid requirements should be raised to 4.5 en% during pregn

How do sponges try to protect themselves against harm, How do sponges try t...

How do sponges try to protect themselves against harm from the environment? Is that method efficient or rudimentary? Sponges can close their pores to avoid the entrance of wate

How can we control the disease cholera, How can we control the disease Chol...

How can we control the disease Cholera Control: Individuals infected  with cholera require oral rehydration therapy  with NaCl plus sucrose, sodium bicarbonate and potassium ch

Contraceptive mechanism of the iud, Q. What is the contraceptive mechanism ...

Q. What is the contraceptive mechanism of the IUD? The IUD (intrauterine device) is a piece of plastic coated with copper that is put-in within the uterus by a doctor. Coppe

Explain isoniazid, Explain Isoniazid It is the drug of choice for treat...

Explain Isoniazid It is the drug of choice for treatment of latent TB infection. It should be given for 9 months in a single daily dose of 300 mg for adults and 10 mg/kg (max 3

Explain functional property of emulsification, Explain Functional property ...

Explain Functional property of Emulsification Mode of action  Proteins stabilize fat emulsions Food system Sausages, soups, cakes, salad dressings, infant foods

Photosynthesis - responses of plants to stress, Photosynthesis - Responses ...

Photosynthesis - Responses of Plants to Stress Photosynthesis is also inhibited due to low water potential in the leaves. The leaf water potential is the sum of turgor pressur

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd