Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

What will the responses look like expain, You have a neuron with a resting ...

You have a neuron with a resting potential of -55 mV, EK of -70 mV, and ENa of 50 mV. You decide to voltage clamp the neuron. Now, draw the membrane current over time. What will th

What are the main characteristics of the bryophytes, What are the main char...

What are the main characteristics of the bryophytes? Bryophytes are nonvascular plants, i.e., they do not have conductive tissues and they perform transport of water and nutrie

Vitamins requirements for ulcerative colitis, Q. Vitamins requirements for ...

Q. Vitamins requirements for ulcerative colitis? Vitamins: Commercial multivitamin preparation should be administered orally especially the ones needed for the healing process

Define the gel electrophoresis, Define the Gel Electrophoresis? Gel ele...

Define the Gel Electrophoresis? Gel electrophoresis is among the most powerful and yet conveniently used methods of macromolecular separation. The gels in common use, polyacryl

Zoology, Wheel organ of amphioxus.

Wheel organ of amphioxus.

Difference between hazard and disaster, 1.      Hazard Is An Extreme Event ...

1.      Hazard Is An Extreme Event Whereas Disaster Is Its Consequence. 2.      A hazard can occur without human interference but it may become a disaster on account of careless

Keys for identification of taxonomic work, Q. Keys for identification of ta...

Q. Keys for identification of taxonomic work? Identification is an integral part of all taxonomic work. It is the recognition of certain, characters of flower, fruit, leaf or s

Annuloplasty with a ring, Annuloplasty with a Ring :  The lings commo...

Annuloplasty with a Ring :  The lings commonly used are Carpentier- Edwards and Cosgrove's flexible half ring. The rings are C shaped and have incomplete circles to accommoda

How dissimilar are walls of these two types of blood vessel, Are the arteri...

Are the arteries or the veins constituted of more muscle tissue? How dissimilar are the walls of these two types of blood vessels? The arterial system has thicker muscle walls

Pluripotent scs:grouping of stem cells, Pluripotent SCs : Characterizing...

Pluripotent SCs : Characterizing more restricted abilities for differentiation.their sources are some celles blastocyst (5-14 days) embroys, so can from over 200 cells types i.e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd