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Genomic libraries are screened by hybridization with a DNA probe which is complementary to component of the nucleotide sequence of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone. Exchange approach is possible if some of the protein sequence for the preferred gene is known. Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.
Explain Theory or Principle of Determination of Coliforms? Coliforms are gram negative, non-spore forming, facultative anaerobic bacteria which produce acid and gas on lactose
DERMIS (CORIUM) - Mesodermal in origin. More developed part. Leather is obtained from dermis by tanning. Thinnest in eye lid. Connective tissue present. In it 4 types
Autoradiography Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion
1. Hazard Is An Extreme Event Whereas Disaster Is Its Consequence. 2. A hazard can occur without human interference but it may become a disaster on account of careless
Explain Atrial Switch Operation ? The hospital mortality reported varies between 0 and 15 per cent. Late survival is worse for TGA with VSD compared to simple TGA. 15 year surv
What is Cloning Cloning: Somatic (ie 2N) cell from original female cat removed (nucleus has mutated allele), fertilised egg extracted from another cat, nucleus removed, and egg
How the Wet beriberi Developed Rapidly? Oedema is the significant feature of wet beriberi. It may develop rapidly and involve not only the legs but also the face, trunk and ser
Stages of Wilm's tumour We shall now discuss about the various stages of Wilm's tumour. The extent of disease is staged according to the findings of surgery and the presen
Adverse Effects of Bronchospasm Nasal and throat discomfort can occur. Bronchospasm, sometimes severe, has been reported uncommonly in patients with reactive airway disease; za
Explain Need For Microbiological Analysis of Environment Samples? Once the food is harvested, it passes through various operating steps before reaching the consumer. These step
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