Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

#title., poor metabolism phenotype will have

poor metabolism phenotype will have

Explain nongonococcal urethritis, Nongonococcal nonchlamydial urethritis an...

Nongonococcal nonchlamydial urethritis and cervicitis Nongonococcal urethritis (NGU) in men is often nonchlamydial as well. Possibly caused by Ureaplasma urealyticum, Mycoplas

Can you explain shellfish poisoning, Q. Can you explain Shellfish Poisoning...

Q. Can you explain Shellfish Poisoning? Shellfish like oysters, mussels and clams are generally bred in the sewage polluted beds or brackish water. The shellfish poisoni

Mechanism of respiration in cockroach, MECHANIS M - Tergo-sternal m...

MECHANIS M - Tergo-sternal muscles are relaxed. Volume of cavity is increased and pressure is decreased. To full fill the pressure air comes through the passage & mixed

Limnology, Limnology : It is the study of organisms present in rivers or fr...

Limnology : It is the study of organisms present in rivers or fresh water lakes. Limnology is also called as freshwater science. This is the study of inland waters. Limnology is of

Explain the disadvantage of contact plate method, Explain the Disadvantage ...

Explain the Disadvantage of Contact Plate Method? These plates are suitable for sampling the surfaces having low contamination, like pre-cleaned and sanitized surfaces. This is

Define points to keep in mind while feeding a preschooler, Define Points to...

Define Points to Keep in Mind while feeding a preschooler? 1. Do not force-feed the child. 2. Children naturally eat a variety of foods. Their sensory-specific satiety ensur

Amylase stock solution, Assume that you have 1 mL of a solution amylase (an...

Assume that you have 1 mL of a solution amylase (an enzyme) at a concentration of 15 mg protein/mL. Calculate the volume of diluting buffer that you would have to add to 1.0 mL of

What are the cells that form the cartilaginous tissue, What are the cells t...

What are the cells that form the cartilaginous tissue? The main cells of the cartilages are the chondrocytes, originate from the chondroblasts that secrete the intersticial ma

Problem with role of lymphatic system, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd