Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Causes of deforestation, Other Causes - Deforestation Natural enemies ...

Other Causes - Deforestation Natural enemies like termites, pests and several kinds of diseases affect the forests adversely. There is also massive destruction of forests beca

Molecular hydrogen, Molecular Hydrogen N 2 -fixing organisms also prod...

Molecular Hydrogen N 2 -fixing organisms also produce a membrane bound enzyme called uptake-hydrogenase under N 2 -fixing condition. The physiological significance of the pres

Silver point extended below canal orifice-endodontics, Silver point extende...

Silver point extended below canal orifice: - This discrepancy between the round silver wire and an irregularly shaped canal provides the clinician with an opportunity to use so

Orifices of heart, Atrio-ventricular Orifices The right and left atrio-v...

Atrio-ventricular Orifices The right and left atrio-ventricular orifices are oval apertures. The openings lie in a plane that is almost vertical with a slight downward inclinati

Define the terms transcriptomics and transcriptome, 1. One of the greates c...

1. One of the greates challenges following the human genome project is to understand how genes are regulated and what functions they perform. a. Define the terms 'Transcriptomic

Define starch retrogradation, Starch Retrogradation The starch paste or...

Starch Retrogradation The starch paste or solution obtained after the gelatinisation is not stable and generally produces a viscoelastic, firm  and rigid gel. Structural transf

#title a&p, list all of the parts of a generalized cell that might be invol...

list all of the parts of a generalized cell that might be involved support movement coordination respiration digestion transportation excr production making proteins and reproducti

Explain about the proteins, Explain about the Proteins? The term protei...

Explain about the Proteins? The term protein (Greek: proleuo- to occupy first place) was first suggested by Berzelius to describe the complex organic nitrogenous substances fou

What is low - density lipoprotein subfractions, LDL is heterogeneous (Kraus...

LDL is heterogeneous (Krauss and Burke, 1982) and can be separated on density gradient ultracentifugation into subclasses that vary in size, density and lipid content. In healthy s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd