Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Phylum , General characteristics of phylum

General characteristics of phylum

What are the cells with a delimited nucleus, Q. What are the cells with a d...

Q. What are the cells with a delimited nucleus called and what are the major elements of the nucleus? Cells with delimited nucleus are known eukaryotic cells. Organisms compose

What is enzyme assay, What is Enzyme assay Enzyme assay may also be use...

What is Enzyme assay Enzyme assay may also be used to determine whether stored plant products are suitable for use as food commodities, for e.g.  α-amylase should be present

What is the first polar body, Q. What is the first polar body? How differen...

Q. What is the first polar body? How different is it from the oocyte II? In oogenesis the oogonium differentiates into oocyte I (2n) and this cell enters meiosis and after fini

What is the meaning of darwins expression, What is the meaning of Darwin's ...

What is the meaning of Darwin's expression "descent with modification"? Descent with modification refers to natural selection. Descent with modification refers to evolutionary chan

Arctic tundra, The northern most biome on our planet is the arctic tundra. ...

The northern most biome on our planet is the arctic tundra. It is a treeless wet circumpolar band between the polar ice caps and the forests to the south. The predominant plants

Write short note on cholesterol, Cholesterol, from stereos (solid) and the...

Cholesterol, from stereos (solid) and the Greek chole- (bile) followed by the chemical suffix -ol for an alcohol is an organic chemical substance classified as a waxy steroid of fa

Evaluating the degree of urinary obstruction, Evaluating the degree of urin...

Evaluating the degree of urinary obstruction: D.P. is a 63-year-old man who has been experiencing progressive difficulty with initiating the urinary stream and frequently needs to

Advantages of implant supported maxillo-facial prosthesis, Q. Discuss the a...

Q. Discuss the advantages of implant supported maxillo-facial prosthesis. Maxillofacial Prosthodontics involves the prosthetic rehabilitation of patients with congenital or acq

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd