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Most RNA molecules are single-stranded but an RNA molecule should hold regions that can form complementary base pairing where the RNA strand loops back on itself in the above figure. If so, the RNA will have few double-stranded regions. The Ribosomal RNAs (rRNAs) and transmit RNAs (tRNAs) exhibit substantial secondary structure, as do some messenger RNAs (mRNAs).
figure: An example of self-complementarity in RNA, forming an internal double-stranded region; hydrogen bonding between bases is shown by the symbol •.
Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitroc
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