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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Which of Mendel's postulates can only be demonstrated in crosses involving at least two pairs of traits? a) Segregation b) dominance/recessiveness c) independent assortment d) unit
Q. Discuss the role of dental implants in orthodontic anchorage? The increasing popularity of dental implants has led to its use for orthodontic anchorage. Conventional means o
What is Ancient Gondwanaland? Did you know that the land surface of the earth was once comprised of two super continents called Gondwana and Laurasia? Click on the Multimedia b
What is meant by encystment
Why are heterotrophic, autotrophic , and mixotrophic protists not classified as plants or animals ?
EVIDENCES OF EVOLUTION - The evidences of organic evolution derived from many lines of study indicate that present day forms have arisen by gradual changes from pre existing fo
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Mitral Valve Replacement : When mitral valve replacement for a calcified, stenotic valve is done, usually it is a classical procedure removing leaflets, their chordae an
Obesity a health hazard: The factors of obesity are complex. Obesity is due to over eating and excess of energy intake. The obese child when grows up will be a target
Microorganisms that survived the preservation and shortage treatment applied to the food Different microorganisms have different susceptibility to the preservative methods empl
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