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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Define Nutrition Support in elderly - Parenteral, Enteral and Oral? Under nutrition either due to dietary deficit a due to a medical complication may arise in the elderly. The
Q. Where are the adrenal glands located? How many are they and what are their portions? Each adrenal gland is located on the top of each kidney (forming a hat-like structure fo
Determination of Vitamin A Vitamin A assay is carried out by chemical method. This method is called Carr Price Reaction. Vitamin A and the carotenoids produce an intense b
Ask questiRed-green color blindness is an X-linked recessive disorder. If Allison is heterozygous (a carrier), and her husband, Michael, is NOT colorblind. What is the chance that
Considering a pair of homologous chromosomes containing a gene having two different alleles how many different genotypes can the individual present?
What are primary consumers? Can a food chain present quaternary consumers without having secondary or tertiary consumers? Can a tertiary consumer of one chain be a primary or secon
Endocytosis and Exocytosis Endocytosis is the process by which substances are brought into the cell from outside by formation of membrane bound vesicles. These extra cellular sub
Carbohydrates These form about 1%part of protoplasm, they are comparatively simpler compounds of carbon, hydrogen and oxygen in the ratio 1:2:1 The ratio hydrogen to oxygen is
Q. DNA damaged by alkylating agents? 1. Some simple alkylating agents 2. Illustrations of products of alkylating agents 3.Many of these products can be repaired by excision rep
Define Improvement of cereal proteins - Mutual supplementation? Improvement of cereal proteins: Cereals, in general, are limiting in lysine and threonine while legumes, milk, m
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