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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
TYPES OF EGGS Different species of animal has different type of eggs. They are classified on the following basis - 1 . ON THE BASIS OF AMOUNT OF YOLK On the ba
Epiboly of Ectoderm Like the presumptive endodermal notochordal and mesodermal cells migrate inward the areas in the epiblast vacated through them are occupied by expansion (
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What are bacteriophages? Bacteriophages are viruses specialized in parasitism of bacteria. They are used in genetic engineering as molecular cloning vehicles to insert recombin
Explain puewabilitation programme in details ? Education, counselling, and behavioural interventions are important elements of cardiac rehabilitation. In this guideline, "educ
Is it possible for any son of a couple formed by a hemophilic man (XhY) and a nonhemophilic noncarrier (XX) woman to be hemophilic? If the mothers are not affected by the disea
Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other
Q. Do bacteria cells have a nucleus? In bacteria the genetic material is dispersed in the cytosol and there is no inner membrane that delimits a nucleus.
Introduction: There are different technologies available for dealing with waste water management and excreta. Some are suitable for dealing off-site sanitation and other can b
Define Proteins as Enzymes? From conception to death, living cells use oxygen and metabolize fuel. Cells synthesize new products, degrade others, and generally are in a state o
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