Restriction endonuclease, Biology

Assignment Help:

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....

Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?

Answer

To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.


Related Discussions:- Restriction endonuclease

Scanning electron microscopy, Scanning Electron Microscopy (SEM) While 'T...

Scanning Electron Microscopy (SEM) While 'TEM is useful to study the internal structul-c of thc ccll. SEM is an important technique if you wish to examine the surl'ace features o

How phytohormones help development of parthenocarpic fruits, How phytohormo...

How phytohormones help the development of parthenocarpic fruits? The Parthenocarpic fruits are those produced with no fecundation. Several plants naturally make parthenocarpic

Show species with exoskeleton, Q. Which other phylum of the animal kingdom ...

Q. Which other phylum of the animal kingdom present species with exoskeleton? Arthropods present exoskeleton made of chitin. Echinoderms do not have exoskeleton but they presen

Homoeostasis, what stimulate the system in homoeostasis

what stimulate the system in homoeostasis

Percussion - criteria for endosteal implants, Percussion - criteria for end...

Percussion - criteria for endosteal implants? It is neither an indicator of clinical health nor of rigid fixation. Ringing sound that occurs on percussion corresponds to the pr

Occlusal load affect osseointegration, Occlusal load affect Osseointegratio...

Occlusal load affect Osseointegration The timing and amount of overload are critical for osseointegration. Occlusal loading early, where the primary stability is low leads to h

Determine the term - neuropsychology, Determine the term - Neuropsychology ...

Determine the term - Neuropsychology The neuropsychology of the various modalities and domains involves applications from the knowledge base concerning the domain or modality i

Healthcare delivery - risk pooling, Normal 0 false false fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Which molecules has more direct effect, Which of the following molecules ha...

Which of the following molecules has more direct effect in learning and memory? A) norepinephrine B) epinephrine C) glutamate D) acetylcholine E) glycine Please leave justification

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd