Restriction endonuclease, Biology

Assignment Help:

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....

Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?

Answer

To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.


Related Discussions:- Restriction endonuclease

Anaerobic lagoon system, A n a e r o b i c lagoon system This s...

A n a e r o b i c lagoon system This specific system of liquid manure handling is restricted to organized dairy farms, where the flushed manure is directed into an anae

Skeletal system - cerebro-spinal fluid, Cerebro-spinal Fluid - Formed m...

Cerebro-spinal Fluid - Formed mainly by choroid plexus and ependyma of ventricles. It comes out of medulla oblongeta by foramen of magandie and lushka . Slightly alkalin

What is an illustration of gene control, Q. What is an illustration of gene...

Q. What is an illustration of gene control? An elegant instance of gene control in bacteria is operon system. A cluster of genes which is responsible for synthesizing a particu

Why soybean protein used as stabilized dispersions, Soybean protein used as...

Soybean protein used as stabilized dispersions Soybean protein concentrates have been used as stabilized dispersions in milk-like beverages and simulated dairy products, such a

Explain the food intolerance, Explain the Food Intolerance? What is foo...

Explain the Food Intolerance? What is food intolerance? How does it differ from food allergy? Food intolerance like food allergy is an adverse reaction to food. Food intoleranc

Importance of the emergence of seeds in the plant kingdom, Q. What is the e...

Q. What is the evolutionary importance of the emergence of seeds in the plant kingdom? The evolutionary significance of the seed is related to the plant capability of distant c

Explain water universal solvent, Explain water Universal Solvent ? Wa...

Explain water Universal Solvent ? Water - Universal Solvent :  Water is usually referred to as the "universal solvent." because it is able to dissolve many different substan

Trypanosomiasis, Trypanosomiasis The trypanosomiasis, also known as Af...

Trypanosomiasis The trypanosomiasis, also known as African sleeping sickness or Chaga’s disease, is caused by Trypanosoma cruzi, T. gambiense and T. rhodesience. T. evansi cau

State the aim of neuropsychological assessment, State the aim of Neuropsych...

State the aim of Neuropsychological assessment Neuropsychological assessments aim at specifying in as much detail as possible the functional deficits that exists in a manner th

Control of glycogen metabolism, The major factor which controls glycogen me...

The major factor which controls glycogen metabolism in the liver is the concentration of phorphorylase alpha. Certainly, this enzyme catalyzes the limiting step of glycogen breakdo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd