Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
Gene targeting in somatic cells: Since the pioneering work with sheep at Roslin cloning by nuclear transfer has now been exemplified in a number of other livestock species includ
Q. What is Acute Aortic Regurgitation? Infective endocarditis, aortic dissection and trauma often produce severe AR. Acute increase in left ventricular end diastolic volume es
Why Fats, Oils and Sugar are important for human body? This group imparts flavour to the items and thereby improves the palatability. Items incorporating them do melt in the mo
Prokaryotes and Eukaryotes By now, you know that cell theory holds good for all living forms. However, if you examine under the microscope, the cells of a bacterium, a blue gr
Explain the Expanding Frontiers of Nutrition? While the essential nutrients mentioned in Table are indispensable and our diets need to provide them all in adequate quantities,
Unified Cell Theory The present unified cell theory states that All living things are composed of either a single Cell or of the cells and cell products. The cell is a m
Maturation and adaptation: (18 to 54 weeks) The final stage involves maturation and adaptation of the implant-bone interface, peri-implant bone and the entire implant supportin
1. Name four experimental techniques to determine protein-protein interactions other than X-ray & NMR techniques. 2. How common are Coiled-coil motifs? Where are they found? Giv
State the terms - Blastocoel and Blastopore? During the development of embryo, single cells divide and form a hollow ball of cells one cell layer thick. Cavity inside this holl
How do birds reproduce? Birds, like each vertebrate, have sexual reproduction. Their embryos develop within shelled eggs having extraembryonic membranes and outside the mother'
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd