Restriction endonuclease, Biology

Assignment Help:

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....

Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?

Answer

To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.


Related Discussions:- Restriction endonuclease

Define work capacity that is relevant to sports nutrition, Define Work Capa...

Define Work Capacity that is relevant to sports nutrition? There are generally two areas of work capacity that is relevant to sports nutrition one is aerobic capacity and the o

Define the concept of food security, Define the Understanding the Concept o...

Define the Understanding the Concept of Food Security? Food security may be defined as a physical and economic access by all people, at all times, to sufficient food to meet th

Biochemistry, help me in writing assignment on racemization,mutarotation.

help me in writing assignment on racemization,mutarotation.

Is total volume of the reaction is 30 micro liters, You have a 10x enzyme b...

You have a 10x enzyme buffer that you are to use in a particular reaction. The total volume of the reaction is 30 micro liters. How much of the 10x buffer should you add so that th

What are good pharmacovigilance process, Question 1: What are Good Phar...

Question 1: What are Good Pharmacovigilance Process and Good Post-Marketing Surveillance Process? How do they differ from each other? Define and illustrate briefly Good Post

A balanced diet must contain enough energy, A balanced diet must have enoug...

A balanced diet must have enough energy to meet the body's needs. What else must it have?    In addition to sufficient energy, a balanced diet must have proteins, car

Bergmann's method of cell plating, Bergmann's Method of Cell Plating I...

Bergmann's Method of Cell Plating In this method free cells are suspended in a liquid medium at a density twice the finally desired plating density. Melted agar containing med

Explain brownian movement, Explain brownian movement When you view a so...

Explain brownian movement When you view a sol through an ultra microscope, you will observe that colloidal particles appear to be in a state of rapid and irregular motion calle

Chlamydiosis-clinical manifestations, Clinical manifestations Since the...

Clinical manifestations Since the disease due to Chlamydia involves many organs/systems, system-wise descriptions of clinical signs is described as under: Genital infectio

What are the destinations of those oxygen atoms, Photosynthesis is the most...

Photosynthesis is the most significant producer of molecular oxygen (O2) on our planet. From which molecule do oxygen atoms liberated by photosynthesis come? From which other molec

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd