Restriction endonuclease, Biology

Assignment Help:

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....

Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?

Answer

To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.


Related Discussions:- Restriction endonuclease

Use of aldehydes as chemical sterilant, Q. Use of Aldehydes as chemical ste...

Q. Use of Aldehydes as chemical sterilant? Formaldehyde: widely employed for the fumigation of operation theatres. Glutaraldehyde: commercial preparations are active at

Explain kingdom monera, Kingdom Monera The monerians are structurally t...

Kingdom Monera The monerians are structurally the simplest of all living things. They have apparently changed little since they first appeared on the earth. Their structure is

What do you mean by myocarditis, Q. What do you mean by Myocarditis? My...

Q. What do you mean by Myocarditis? Myocarditis is defined as inflammation of the myocardium. The most common cause is Coxsackie B virus infection. But it can also be due to

Tooth associated with persistent apical periodontitis, Tooth associated wit...

Tooth associated with Persistent apical periodontitis Functional retention of the tooth : persistent lesion while remain asymptomatic function for an extended period of time .

Source of nutrients and energy of microorganisms, Q. Source of nutrients an...

Q. Source of nutrients and energy of Microorganisms? Microorganisms use foods as a source of nutrients and energy. Each kind of microorganism has a definite range of food requi

What is conventional breeding, What is Conventional breeding Conventio...

What is Conventional breeding Conventional breeding is a process in which genes for pesticidal traits are introduced into a plant by natural methods, such as cross-pollination

Structure of the intestines, Describe the structure of the intestines. How ...

Describe the structure of the intestines. How are they modified to carryout their functions? How does the human intestine compare to the fetal pig?

Determine the wechsler intelligence scales, Determine the Wechsler Intellig...

Determine the Wechsler Intelligence Scales This test is given according to manual instructions and is not modified in any way. Most clinicians use the most current revision of

Adverse effects of air pollution, Air pollution affects both living and non...

Air pollution affects both living and non-living matter. The effects can be classified as.

Explain the bases, The bases in DNA have carbon-nitrogen ring structures; d...

The bases in DNA have carbon-nitrogen ring structures; due to the nitrogen atoms they are known as nitrogenous bases. There are two parts of ring structure. Adenine and guanine are

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd