Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
F A TT Y ACIDS They are monocarboxylic organic acids (R.COOH) having a hydrocarbon chain of 4 - 30 carbon atoms. The polar carboxylic group is hydrophilic (Gk. hydro
A complete motor neuron is removed from a frog and placed in a large volume of normal physiological saline. The neuron is healthy; it has a stable resting voltage of -70 mill
Describe Wide Split S in details? It is a persistent split that widens on inspiration to at least 60 msec. May be due to delay in P, e.g. RBBB or due to early A, as occasionall
Threatened Species - Wildlife Many plant and animal species are threatened by the possibility of extinction. However, the seriousness of the threat varies. For example, a spec
Describe the respiratory organ in pila
Prepared Assignments on glycolysis
Q. Incineration Treatment technologies for health-care waste? It used to be the method of choice for most hazardous health-care wastes and is still widely used. It is a high te
Describe the monomer unit of a protein and how monomer units are assembled into peptides.
Q. Do all molecules of DNA have the same amounts of nitrogenous bases? Number and sequence of nitrogenous bases in DNA molecule are key elements in variations which are found i
The reported occurrence of ICMAs is 1.2 per cent to 5 per cent of cases. Streptococci and S.aureus account for 50 per cent and 10 per cent of cases, respectively, and are seen with
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd