Restriction endonuclease, Biology

Assignment Help:

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....

Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?

Answer

To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.


Related Discussions:- Restriction endonuclease

What is gymnosperms explain its dividions, What is Gymnosperms explain its ...

What is Gymnosperms explain its dividions? Gymnosperms are one of the two major groups of plants that bear seeds. The other group, the Angiosperms (flowering plants), differ in

Explain the periapical surgery - endodontic surgery, Explain the Periapical...

Explain the Periapical surgery - Endodontic Surgery a) Curretage 1 b) Root-end ressection 2 c) Root-end preparation 3 d) Root-end filling 4

Evolution of metazoa, Evolution of Metazoa The sponges, coming under p...

Evolution of Metazoa The sponges, coming under phylum Porifera are the closest to Protista, and can perhaps be regarded even as a colony of protists rather than being multicel

What is hemoglobin s, Question 1 What is megalobastic anemia? Discuss brie...

Question 1 What is megalobastic anemia? Discuss briefly its lab diagnosis. How would you differentiate megaloblastic anemia from other anemias? Question 2 What is hemog

How low birth weight cause protein energy malnutrition, How Low Birth Weigh...

How Low Birth Weight cause Protein Energy Malnutrition? The beginning of PEM in children starts in US from the time of their birth. At least a third of the Indian children are

Animal kingdom, If porifers shiw cell aggregate plan tgen how can they also...

If porifers shiw cell aggregate plan tgen how can they also shiw division of labour among cells

Preparing financial statements from the work sheet, When the work sheet is ...

When the work sheet is finished all the essential information to prepare the statement of retained earnings, income statement and balance sheet is readily available. Currently you

The hydrogen ion concentration of stomach, What is the hydrogen ion concent...

What is the hydrogen ion concentration of stomach acid pH=1.6?

Types of quantitative analyses in nutritional biochemistry, Define Types of...

Define Types of Quantitative Analyses in Nutritional Biochemistry? Methods of quantitative analysis broadly there are two types of quantitative analyses: (a) Gravimetric,

Microbiology of air, Ask question #i need the complete table having the nam...

Ask question #i need the complete table having the names of the diseases their causative agents and their control,caused by air-borne pathogens?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd