Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
External Respiration: Diffusion of oxygen into blood and carbon dioxide into alveoli (external respiration) is the diffusion of oxygen from air in the alveoli of lungs to bl
Q. What is packaging? What is packaging? Packaging means a coordinated system of preparation of goods (in this case, foods) for shipment, distribution, storage and
how pollination occurs in Salvia?
Define influence of polyphenols on Carbohydrates? Binding of proteins may indirectly affect carbohydrate absorption. Inhibition of amylolytic enzymes and subsequent reduction o
How much Fluids should be taken for management of obesity? Liberal amounts of water and zero/low calorie fluids may be included in the diet. It may be helpful to have a glass o
Long-hair in cats is due to the recessive long-hair allele, l. Black cats (rather than tabbies) are due to the recessive agouti allele, a. A long-haired black female cat has mat
Q. Symptoms of mitral regurgitation? Symptoms depend upon underlying etiology of mitral regurgitation. Patients with mild mitral regurgitation and most of those with even sever
Q. Why we use Histamine in consciousness? Histamine is involved in controlling the level of consciousness during waking. The level of histamine diminishes during sleep and its
Define the Sterilization protocol? Sterilization protocol encompasses the following: 1. Transport of instruments to the sterilization area 2. Cleaning of instruments 3
Chemical Composition and Nutritive Values The nutritional quality of an oil meal protein will be affected by the processing conditions to which it is been exposed. Three major
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd