Restriction endonuclease, Biology

Assignment Help:

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....

Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?

Answer

To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.


Related Discussions:- Restriction endonuclease

Explain the copper toxicity in human, Explain the Copper Toxicity in Human?...

Explain the Copper Toxicity in Human? Acute copper toxicity in humans is rare and occurs due to inadvertent consumption of copper salts. Symptoms include vomiting, diarrhoea, h

Solutions, how many grams of table sugar would you need to dissolved in wat...

how many grams of table sugar would you need to dissolved in water to produce 0.75 liters of a 0:25 molar acqeous solution of table sugar?

Explain the term - neurochemical manipulations, Explain the term - Neuroche...

Explain the term - Neurochemical Manipulations Neurochemical and immunological methods have been used to identify groups of neurons in the central nervous system that use speci

Electron transport chain, ELECTRON TRANSPORT CHAIN All processes requir...

ELECTRON TRANSPORT CHAIN All processes require energy. In living cells, we constantly use energy for a number of biochemical reactions e.g. muscular movements, synthesis  of ne

While meiosis results in four diploid cells, Which of the following best co...

Which of the following best compares the results of mitosis and meiosis? Mitosis results in four diploid cells, while meiosis results in two haploid cells. Mitosis results in four

Causes of heart failure, It is important to recognise underlying causes and...

It is important to recognise underlying causes and precipitating factors of heart failure for its appropriate management. That would also help in prevention and treatment of heart

How do contraceptive pills generally work, How do contraceptive pills gener...

How do contraceptive pills generally work? Contraceptive pills generally have the hormones estrogen and progesterone. If taken daily from the 4th day after menses the abnormal

Digestive system - small intestine, SMAL L INTESTINE - A narrow tube 6...

SMAL L INTESTINE - A narrow tube 6 m long. In rabbit 4 m. long.Differentiated into duodenum, jejunum & Ileum. (i) Duodenum - 2.5 cm long. C-shaped (U-shaped in rabbit)

Amoeba, AMOEBA Systematic  Position Amoeba  was discovered by  Rus...

AMOEBA Systematic  Position Amoeba  was discovered by  Russel von Rosenhoff in  1755. He  called it the little proteus  after the mythical  sea god who is believed to be c

What is consanguineal marriage, What is consanguineal marriage? Why is the ...

What is consanguineal marriage? Why is the appearing of genetic disease more probable in the offspring of a consanguineal marriage? Consanguineal marriage is the marriage among

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd