Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
How cells are studied We learnt about the evolution of the cell and a historical account of the growth of cell biology. In this section, you will study about the various tools
Describe about the extra-ocular muscles The extra-ocular muscles are mainly involved in the maintenance of postural tonicity and performance of quick contractions. The prima
Estrus - Estrous cycle This is the period of heat, and copulation is permitted only at this time. This condition lasts from 9 to 15 hours and is characterised by a high rate o
Circulation, Respiration and Excretion of Asteroidea Asteroids depend upon the circulation of coelomic fluid for the transport of gases and some nutrients. The blood vascular
Avian influenza The etiological a gentisa via ninfluenz a type A virus und e r the family Orthomyxoviridae. Avian influenza pandemics in Pakistan, Nepal and Myanamar (1995) and
Q. Write the meaning of Hyperglycemia? Hyperglycemia is a Greek term: hyper -meaning excessive; glyc - meaning sweet; and emia- means "of the blood". It is a condition in whic
How Surgical technique affect Osseointegration The osteotomy preparation is critical both from biologic and biomechanical points of view. The bone drilling should be sequential
What are brief descriptions of how you could test hypothesis. or how would you know if your hypothesis is testable?
What is the physical experiment that show that the protoplasm has contractility?
Migration in birds
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd