Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
Define Proteins in the immune system? Proteins such as γ-globulin serve to protect the body against foreign cells. The immunoglobulin produced by lymphocytes is the large polyp
Explain about the Chytridiomycota - Fungi Chytridiomycota - Look at the Figure, which illustrates chytridiomycota. These are the simplest among true fungi and are commonly call
Q. What are the three main parts into which a neuron can be divided? What are their respective functions? The three mains parts into which a neuron can be didactically divided
Significance of Mitosis 1. Reproduction ; William Bateson 1906 wrote, Whenever I observe a dividing cell under the microscope , I feel as if the original act of c
Cardiac Output - Circulation The cardiac output can be determined in a number of ways. The most common method is the Fick's principle. A. Fick a German physiologist in 1870 de
External Respiration: Diffusion of oxygen into blood and carbon dioxide into alveoli (external respiration) is the diffusion of oxygen from air in the alveoli of lungs to bl
Anther is the top of a stamen's filament; divided into pollen sacs in which the pollen grains are form.
Is the effect of genetic drift likely to be the same in pop 1 and pop 2? How are genetic drift and pop size related? when there is strong selection against the homozygous recessive
Define Modification of Carbohydrate Intake for Specific Disorder? In our daily diet almost 60-7096' of energy is contributed by carbohydrates. Majority of them are comprised of
Patient is a 7-year-old girl who was brought to her physician by her mother with complaints of general fatigue, anorexia, and unexplained bruises and "rash" for the past 2 weeks. C
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd