Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
Explain the Structure of Fungi? The vegetative structure of a fungus is called thallus. It varies in complexity and size, ranging from unicellular yeasts to multicellular mould
NON-EDIBLE AND OTHER MINOR OILSEED CAKES Besides the annually cultivated oilseed crops namely, groundnut, sesame, mustard, linseed, coconut, cotton, sunflower and safflower, I
what are the phase of alpha taxonomy?
what is meant by thigmotropism?
Explain the De Bakey Type I Dissection? De Bakey Type I Dissection : Initial steps of the operation are same as previously described. Provision for deep hypothermic ci
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
Describe Respiratory System? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from the blood. This process is c
Blunt Injury Closed penumothorax Tension pneumothorax Tracheobronchial injury Fracture ribs and flail chest Mediastinal injury
Define Typical Ambient Air Pollutants Particulate matter Sulfur containing compounds Organic compounds Nitrogen containing compounds Carbon monoxide
Q. Use of Aldehydes as chemical sterilant? Formaldehyde: widely employed for the fumigation of operation theatres. Glutaraldehyde: commercial preparations are active at
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd