Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
Pathophysiology Large haemorrhages and fibrinous lesions vegetate along the inflaked edges of valves. The lesions develop on adjacent valve leaflets so that the edges adh
Two gene loci, A and B, assort independently, and alleles A and B are dominant over alleles a and b. Indicate the probablilities of producing .... an AB phenotype from a cross AaBb
Which of the following results from the elimination of DNA ligase activity in a cell? A. The oligonucleotide primers will not be removed from the genome - as they often contain
Define Factors Affecting Microbial Survival and Growth? Microbes commonly present on the food, we have seen, include members of the genera Bacillus, Clostridium, Pseudomonas, S
Which are the organs of the excretory system? The excretory system is produced of kidneys (two), ureters (two), bladder and urethra. The Excretory System - Image Diversity:
State in brief about the soil horizon A soil horizon may be defined as "a horizontal layer of regolith, approximately parallel to the soil surface, and possessing relatively
Central Nervous System Hypertension is one of the leading causes of cerebrovascular disease. It has been associated with accelerated age related cognitive decline. The most d
Q Why is it said that during photosynthesis carbon dioxide is improved to form glucose? During photosynthesis carbon dioxide is energetically improve with hydrogen from water.
What are the parasympathetic neurons Patient A has a disease that has destroyed half of the alpha-adrenergic receptors on each of the smooth muscles that control the diameter o
Define Hepatopoietic and immune System - geriatric nutrition? Though the circulating red blood cells or the white cell count or platelet number does not normally change with a
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd