Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
Le a d It is common cause of poisoning in cattle. Lead poisoning in other species is limited by reduced accessibility, more selective eating habits, or lower susceptibility.
The Starch Granule - Structure Starch is a polysaccharide, a chain of many glucose molecules. There are two types of glucose chains in starch. One is a simple chain called am
Question 1) What is megalobastic anemia? Discuss briefly its lab diagnosis. How would you differentiate megaloblastic anemia from other anemias? Question 2) What is hem
Synergistic and Sequential Effects of Hormones In vitro studies on callus and suspension cultures have brought out two interesting findings. The nature and direction of differ
Contagious bovine pleuropneumonia (CBPP) This is a highly fatal disease of cattle and of major economic importance in certain tropical countries. It also affects buffaloes, bis
Describe the Positive End Expiratory Pressure (PEEP)? A positive pressure may be applied at the end of the expiratory phase during mechanical ventilation, instead of allowing t
Explain the importance of parent material Part of these weathered minerals are transported away by volcanoes, wind, water, ice, and waves etc. These very factors transport th
sanguivarous mode of feeding in annelida
What are trophic levels? How many trophic levels can a food chain have? The Trophic levels correspond to positions on a food chain. thus producers always belong to the first tr
Trout beck zone - Lotic Ecosystem This is larger and more constant than the head stream. The greater volume of torrential water carves channels into exposed rock floor (bed ro
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd