Regents for estimation of vitamin c - colorimetric method, Biology

Assignment Help:

Define Regents for Estimation of Vitamin C - Colorimetric Method?

1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric acid and allow to stand overnight. Mix and use.

2. 5% thiourea solution - Dissolve 25 g of thiourea in 500 ml of distilled water.

3. 9N sulphuric acid

4. 85% sulphuric acid

5. 0.6% copper sulphate

6. Colour reagent or Dinitrophenylhydrazine - thiourea - copper sulphate (DTC) reagent  - Mix

7. 5% trichloroacetic acid (TCA)

8. 5% metaphosphoric acid in 10% acetic acid solution - Mix 1000 ml of 5% metaphosphoric acid and 500 ml of 10% acetic acid.

9. Ice for cooling.


Related Discussions:- Regents for estimation of vitamin c - colorimetric method

Skin puncture - specimen collection, Skin puncture: skin puncture is...

Skin puncture: skin puncture is usually done when small volume of blood is needed (e.g., blood glucose test) to avoid unnecessary venipuncture. However, skin puncture in

Neurological disorders due to imbalanced nutritional intake, Define Neurolo...

Define Neurological disorders arising due to imbalanced nutritional intake (deficiency or excess)? Common examples are the neurological manifestations  of beriberi, pellagra, p

Treatments of coronary, The treatment of hyperlipidemia requires two approa...

The treatment of hyperlipidemia requires two approaches: Therapeutic lifestyle changes (TLC) and medications. To achieve target LDL levels, most patients need both approaches si

Monosaccharides and disaccharides, Monosaccharides are frequently known as...

Monosaccharides are frequently known as single or simple sugars and are the easiest of all carbohydrates. That is the building blocks of all higher carbohydrates. The Monosacchari

Determine improper fit at the abutment - implant interface, Improper fit at...

Improper fit at the abutment/- implant interface It is very vital that the fit of the abutment is crosschecked radiographically prior to final delivery of the prosthesis. The m

Explain the treats bacterial infections, Is a viral infection treated with ...

Is a viral infection treated with the same kind of drug that treats bacterial infections? Antibacterial drugs, potent against a great variety of bacteria, are not effective aga

Which kind of chemical bond maintains the pairing, Q. Which kind of chemica...

Q. Which kind of chemical bond maintains the pairing of each chain in the DNA molecule? To form the DNA molecule, purine bases bind to pyrimidine bases by intermolecular bonds

What do all organic compounds contain, What do all organic compounds contai...

What do all organic compounds contain? The key element is carbon. Organic compounds are all carbon-having compounds. By definition, an organic compound having carbon. Alt

Define drug metabolism (poly-pharmacy), Define Drug metabolism (Poly-pharma...

Define Drug metabolism (Poly-pharmacy)? As older persons are sometimes prescribed number of medications, care is to be taken, as there is reduction in renal and hepatic clearan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd