Regents for estimation of vitamin c - colorimetric method, Biology

Assignment Help:

Define Regents for Estimation of Vitamin C - Colorimetric Method?

1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric acid and allow to stand overnight. Mix and use.

2. 5% thiourea solution - Dissolve 25 g of thiourea in 500 ml of distilled water.

3. 9N sulphuric acid

4. 85% sulphuric acid

5. 0.6% copper sulphate

6. Colour reagent or Dinitrophenylhydrazine - thiourea - copper sulphate (DTC) reagent  - Mix

7. 5% trichloroacetic acid (TCA)

8. 5% metaphosphoric acid in 10% acetic acid solution - Mix 1000 ml of 5% metaphosphoric acid and 500 ml of 10% acetic acid.

9. Ice for cooling.


Related Discussions:- Regents for estimation of vitamin c - colorimetric method

Nutrition, prove oxygen is produced during photosynthesis in the presence o...

prove oxygen is produced during photosynthesis in the presence of light

Solid fuels, Primary and secondary solid fuels are widely used in domestic ...

Primary and secondary solid fuels are widely used in domestic and industrial purpose. Wood and coal constitute the important primary solid fuels. The important secondary solid fuel

Explain membrane filtration technique, Explain Membrane Filtration Techniqu...

Explain Membrane Filtration Technique? Another method used for determining the bacterial number is the filtration technique. The margain figure illustrates the membrane filter

TISSUES, difference between axon and cyton

difference between axon and cyton

Describe the result of pulmonary embolism, Describe the result of Pulmonary...

Describe the result of Pulmonary Embolism? Results :  The mortality in different series is reported between 3 and 36 per cent. In haemodynamically unstable patients this coul

Explain how movement is controlled in protozoans., Movement is even control...

Movement is even controlled by organelles, cilia and flagella. These and the pseudopodia in the amoebas give these small creatures movement, something we associate with animals. Es

Getting ready to grow bacteria, Getting ready to grow bacteria Secure t...

Getting ready to grow bacteria Secure two or three dozen shallow glass dishes. The glass coasters used to stay bed castors from marring floors will do. Cut 5 cm squares of wind

Allele-specific ligation, Allele-specific ligation  is the technique permit...

Allele-specific ligation  is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other

Show the efficient contraceptive method, Q. Why is the use of condoms not j...

Q. Why is the use of condoms not just a contraceptive method but also a health protection behavior? The use of condoms besides being an efficient contraceptive method also help

Relate the oxide layer and biocompatibility of titanium, Relate the oxide l...

Relate the oxide layer and biocompatibility of titanium. The compatibility of a metal with its host environment depends on its resistance to biodegradation and on the degree of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd