Regents for estimation of vitamin c - colorimetric method, Biology

Assignment Help:

Define Regents for Estimation of Vitamin C - Colorimetric Method?

1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric acid and allow to stand overnight. Mix and use.

2. 5% thiourea solution - Dissolve 25 g of thiourea in 500 ml of distilled water.

3. 9N sulphuric acid

4. 85% sulphuric acid

5. 0.6% copper sulphate

6. Colour reagent or Dinitrophenylhydrazine - thiourea - copper sulphate (DTC) reagent  - Mix

7. 5% trichloroacetic acid (TCA)

8. 5% metaphosphoric acid in 10% acetic acid solution - Mix 1000 ml of 5% metaphosphoric acid and 500 ml of 10% acetic acid.

9. Ice for cooling.


Related Discussions:- Regents for estimation of vitamin c - colorimetric method

Determine the general principles for incisions, General Principles for Inci...

General Principles for Incisions The general principles for placing incisions so as to create surgical flaps remain the same regardless of the reason for creating them. These a

Explain advantages and disadvantages of spread plate method, Explain Advant...

Explain Advantages and Disadvantages of Spread Plate Method? The advantages and disadvantages of spread plate method are highlighted next. Advantages   1. It is useful fo

Explain synaptic transmission between neurons, Q. How does synaptic transmi...

Q. How does synaptic transmission between neurons take place? The propagation of the action potential along the axon reaches the region immediately anterior to the presynaptic

Define about the population dynamics, Define about the Population Dynamics?...

Define about the Population Dynamics? In this unit, we are going to study about human population who eventually suffer from these problems. Why do we want to study about human

What is the peripheral nervous system, Define the Peripheral Nervous Syste...

Define the Peripheral Nervous System (PNS) Peripheral nervous system consists of cranial and spinal nerves. There are twelve pairs of cranial nerves. They arise from brain.

What are biopolymers, Q. What are biopolymers? Polymers are macromolecu...

Q. What are biopolymers? Polymers are macromolecules made by the union of several smaller identical molecules, called monomers. Biopolymers are polymers present in the living b

Explain surface properties of proteins, Explain Surface properties of prote...

Explain Surface properties of proteins The surface properties relates primarily to surface tension, a) Emulsification  and b) Foaming characteristics of proteins,

Define classification of protein based on amino acid content, Explain Class...

Explain Classification of Protein based on amino acid content? Nutritionally, amino acids are classified on the basis of the body's ability to synthesize them - as essential (i

Define about the resistant starch, Define about the Resistant Starch? U...

Define about the Resistant Starch? Until 1980's starch was thought to be completely hydrolyzed and absorbed from the small intestine of man, independent of its source, type and

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd