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Define Reagent for Separation of Amino Acid by Paper Chromatography?
The following reagents will be required for carrying out the experiment:
1. Amino acid solution-
Weigh abour 3-4 mg of each amino acid and dissolve it in dil HCl (1-2 drops). Make the volume upto 10 ml.
2. Chromatographic solvent
Take a separating funnel. To this add 500 ml of freshly shaken mixture of equal volumes of water and butanol. Add 60 ml of glacial acetic acid. Mix. Shake. Discard the lower layer. Take the upper layer.
3. Ninhydrin solution
1% ninhydrin in acetone.
Calculate the molar concentration of illinic acid and of its conjugate base illinate ion (in moles/L; M) in a 0.002 M illinate buffer at pH 12.0. The pKa of illinic acid is 6.7. En
Explain Strengthening the public health measures? Parasitic infestations and protozoal infections cause iron deficiency through loss of blood or destruction of red blood cells.
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