Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
how to explain the structure and function of the organelle
Q. What is Acute Severe Mitral Regurgitation? Acute severe mitral regurgitation is a medical emergency. Patient presents with breathlessness and low output state. He may be is
The vertebral bodies are much larger in the lower back than the neck. What is the functional significance of this structural difference?
Q. What is Gestational Diabetes? When a pregnant woman develops diabetes, it is known as gestational diabetes. It occurs in only 1% of the pregnant women. Pregnant women who ha
Q What are the few representatives of the phylum Mollusca? Octopuses, Snails, oysters and squids are examples of molluscs. Q. In which habitats do molluscs live? Mollus
Pathophysiology Leukocytes develop fiom stem cells located in the bone marrow. During leukocyte maturation, cells become committed to specific cell types and develop throu
Enterocoelous pattern – Coelom In enterocoelous development of coelom the mesoderm arises in the embryo as paired lateral pouches growing out from the archenteron. The pouche
Define Physical and Physiological Changes? Every stage has its unique requirements due to different changing needs. With respect to nutrition and health, four different basic a
Q. Concerning permeability what type of membrane is the cell membrane? The cell membrane is a selectively permeable membrane that is it allows the passage of water and some sel
Procedure For Define Percentage of reducing and total sugar by fehling soxhlet? 1. Standard titration (Standardization of Fehling's solution) - Titrate 10 ml of Fehling's solut
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd