Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
The boll weevil, an insect native to Mexico and Central America, is another pest of commercial cotton. When chemical insecticides were first discovered, they proved highly effectiv
Some drugs bind extensively to plasma proteins (Warfarin binds 99%) while others have virtually no binding. Extraction depends on the type of drug - there are dissimilar standar
uses of conductometry in biological experiments
Mineralisation and Humification As a result of physical weathering, the rocks are broken down into smaller particles. But this is not the true soil, and plants cannot grow well
What is a characteristic feature of a carrier protein in a plasma membrane? Carrier proteins are globular proteins which are particular it their action and therefore regulate t
Q. Energy during myocardial infarction? As mentioned above, patient who have recently suffered from an attack of myocardial infarction are hospitalized in the intensive cardiac
Zinc deficiency Zinc is essential for several physiological functions in the body such as protein synthesis, carbohydrate and nucleic acid metabolism, and foetal growth. The de
Which are specialized conductive tissues of the plants? Vascular tissues of the plants are the phloem and the xylem. The Xylem is the plant tissue that forms the vessels that c
Sonology : This is the study of hearing. Sonology is a type of science of neologism which is used to describe the study of sound in a variety of disciplines. In medicine, this term
Is it possible that an X chromosome of a woman can have come from her father? It isn't only possible that an X chromosome of a woman is from her father, it is certain. Each wom
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd