Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

What are the factors to an actual shift in the demand curve, What are the f...

What are the factors to an actual shift in the demand curve? Factors that cause an actual shift within the demand curve are as follows: When prices rise or fall that would c

Prevention and control of staphylococcal food poisoning, Q. Prevention and ...

Q. Prevention and Control of Staphylococcal food poisoning? Staphylococcal food poisoning can be prevented by: 1. Avoiding contamination of food with S. aureus. 2. Prev

# FUNGAL NUTRITION, Write on spoilage of food products by fungi,nutrition,c...

Write on spoilage of food products by fungi,nutrition,condition for growth,consequencies and prevention.

Explain chiasms of homologous chromosomes, Q. What are the "chiasms" of hom...

Q. What are the "chiasms" of homologous chromosomes observe in prophase I? Chiasms are intersections of two tracts in the form of X, The chiasms seen in prophase I are chromoso

What is a vesicle, What is a vesicle? A vesicle is a relatively minute ...

What is a vesicle? A vesicle is a relatively minute intracellular, membrane-enclosed sac that keeps or transports substances

Alfieri repair-technique of operation, Alfieri Repair: This is advised ...

Alfieri Repair: This is advised for ischaenlic mitral regurgitation. In the area of prolapse, the anterior and posterior leaflet edges are approximated with one or two mattress

Embryological evidences of evolution, EMBRYOLOGICAL EVIDENCES - A co...

EMBRYOLOGICAL EVIDENCES - A comparative study of embryology of different groups of animals reveals certain features that provide evidences for organic evolution. There ar

Biomedical application using a microcontroller, Search the web (mainly IEEE...

Search the web (mainly IEEE and ACM) publication databases to find a recent article on a biomedical application using a microcontroller. Clearly cite your reference(s). In your

Bone growth and development calcification of osteoid tissue, Define Bone gr...

Define Bone growth and development calcification of osteoid tissue? Vitamin D participates in metabolic processes associated with bone growth and development. It is involved i

What are the possible sources of human embryonic stem cells, What are the p...

What are the possible sources of human embryonic stem cells? Embryonic stem cells can be derived from individual cells of an early embryo, from blood cells in the umbilical co

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd