Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
Q. What is the life cycle of the hookworms? The Adult hookworms within the human intestine release eggs that are eliminated with the human feces. Under the adequate conditions
steps involved in glycolysis
Explain the Micronutrients Requirements during Sepsis Micronutrients: The requirement of almost all vitamins and certain mineral increases due to infection and inflammation. In
What is capillarity? How this phenomenon is chemically explained? What is the relevance of capillarity for water transport in plants? Capillarity is the phenomenon by which
Types of Ovules Anatropous This is the most common type of ovule in angiosperms in which the ovule undergoes curvature such that the micropylar end comes to lie parallel
Reproductive System of Asteroidea Sexes are separate in several asteroids. There are ten (10) gonads, two in each arm. They emerge tuft-like or as cluster of grapes. Mature go
Explain Cultivation of microorganisms It is for the production of food colourants has attractions, these must be measured against the financial legislative and user constraint
Term Translation or protein synthesis, is quite similar in eukaryotes and prokaryotes. We will look at the details of the process in prokaryotes and consider the differences in euk
Explain Alkaline Butt -carbohydrate utilization pattern test? Alkaline slant (red) and alkaline butt (red) or no change (orange-red) butt- Instead of carbohydrates, peptone in
Explain the Process of IgE-Mediated Allergic Response? Basically, there are three steps involved with the IgE-mediated allergic response These include: Step 1: Sensitizat
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd