Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
Q. High Para-sternal or Ductal View? This view is obtained by sliding transducer one or two spaces upward and medially from PLAX view. This view is specifically used to vi
Functional properties of food lipids Chemically, fats and oils, as you may already know by now, are a combination of glycerol and fatty acids. The glycerol molecule has three s
Please help with the following: 32P is an unstable isotope of phosphorus that has a half-life of 14.29 days. Atoms of this isotope undergo beta decay in which a neutron decays i
How does the structure of a crab relate to its habitat? It's difficult exoskeleton protects it for most predators & keeps water off its flesh. The claws are used for mating due
Q. Describe the complex spatial patterns of biodiversity? To describe the complex spatial patterns of biodiversity, ecologists and biogeographers have found it useful to expres
Would you expect the muscle fibers of the tongue to be striated or smooth? What about the muscle of the diaphragm/ Explain your answer.
differences regarding Tiger Planaria and the Iridescent Phyllodoce
Q. Describe ST Segment Depression? The development of ST-segment depression with exercise is probably the most reliable sign if myocardial ischaemia and appears to be the most
What is global warming? Global warming is the enhance in the temperature of the planet due to accumulation of some gases in the atmosphere, especially gases that retain the sol
Cleavage of fructose-1,6-bisphosphate Aldolase cleaves fructose-1, 6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate in the reversible reaction.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd