Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

Overall senescence - senescence, Overall senescence - Senescence Only ...

Overall senescence - Senescence Only the parts above the ground level i.e.  The aerial parts die whereas the underground parts survive For example, potato.

Another type of soil for bacteria gardens, Another type of soil for bacteri...

Another type of soil for bacteria gardens Boil some rice or potatoes in a dish unless well cooked. Drain and save the water. Use the bouillon cube to the gelatin. Use th

The luria-nebraska neuropsychological battery, The luria-nebraska neuropsyc...

The luria-nebraska neuropsychological battery This procedure was first reported in 1978 in the form of two initial validity studies. Historically, Chirstensen, a student of the

Unit membrane model, Unit Membrane Model Lamellar model was supported ...

Unit Membrane Model Lamellar model was supported by Robertson. According to Robertson - All biomembrane have similar structure (like PM), on the basis of this observati

What do you understand by pericardium, Q. What do you understand by Pericar...

Q. What do you understand by Pericardium? Pericardium is the covering sac of the heart. The pericardium prevents acute cardiac chamber dilatation. Acute pericarditis, pericardi

Explain about the cyanocobalamin, Explain about the Cyanocobalamin (vitamin...

Explain about the Cyanocobalamin (vitamin B 12 )? Vitamin B 12 (cobalamin, cbl) is a unique vitamin in human nutrition, since its malabsorption leads to the fatal syndrome of

Photosynthesis, test to prove that carbondioxide is needed for photosynthes...

test to prove that carbondioxide is needed for photosynthesis to take place

Types of cardiac dopler, There are two types of Doppler examination: 1) ...

There are two types of Doppler examination: 1)  Pulse wave Doppler 2)  Continuous wave Doppler Both these modalities are necessary part of Doppler echocardiography and pro

What treatment should be used for cardiac tamponade, Q. What Treatment shou...

Q. What Treatment should be used for cardiac tamponade? Percutaneous Pericardiocentesis Cardiac tamponade is an acute emergency and percutaneous pericardiocentesis must b

Sieve tubes, What are sieve tubes suited for translocation of food? a.bord...

What are sieve tubes suited for translocation of food? a.bordered pits b.no end walls c.bordered lumen and perforated cross walls D.no protoplasm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd