Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

Chronic bronchitis, Chronic Bronchitis Chronic Bronchitis is defined ...

Chronic Bronchitis Chronic Bronchitis is defined clinically as hypersecretion of mucus and recurrent episode of productive cough for a period of 3 months per year at least tw

Features of estuaries, Features of Estuaries The physicochemical prop...

Features of Estuaries The physicochemical properties of the estuaries have large variation in several parameters and this often creates stressful environment for organisms. T

What is orthopedic, Orthopedic Prophylactic antistaphylococcal drugs ad...

Orthopedic Prophylactic antistaphylococcal drugs administered preoperatively can decrease the incidence of both early and late infection following joint replacement. They also

What mechanism of regulation utilized in maintaining, What mechanism of reg...

What mechanism of regulation is utilized in maintaining homeostatic balance of sugar?

I dont know, What are two specific organs that work together to preform a f...

What are two specific organs that work together to preform a function??

What is mutualism, Q. What is mutualism? The Mutualism is the ecologica...

Q. What is mutualism? The Mutualism is the ecological interaction in which both participants advantage and that is obligatory for their survival. The Mutualism is a harmonio

Show the value of embryology, Q. Show the value of embryology? The valu...

Q. Show the value of embryology? The value of embryology in solving taxonomic problems can be appreciated by studying some specific cases. Amongst the classical examples cited

Effects of cardiac output and hormones, Excluding the effects of cardiac ou...

Excluding the effects of cardiac output and hormones, describe the other factors that may affect blood pressure and blood flow in a middle-aged man who is exercising in an aerobics

Pathogenesis of acute rheumatic fever, You have learnt about the epidemiolo...

You have learnt about the epidemiology, etiology and pathogenesis of acute rheumatic fever. Revised Jones criteria, which have major and minor criteria, are helping to diagnosis of

Define about the yeast - saccharomyces, Define about the Yeast - Saccharomy...

Define about the Yeast - Saccharomyces? Classification Kingdom - Mycetae Division - Amastigomycota Class - Ascomycetes Order - Endomycetales Family - Saccharomyc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd