Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

Dietary tips for hyperlipideinic patients, Q. Dietary tips for hyperlipidei...

Q. Dietary tips for hyperlipideinic patients? It is important to note here that the dietary modifications need to be individual in each patient and these should be tailored lo

Magnesium - mineral elements, Magnesium -  It is available in most of t...

Magnesium -  It is available in most of the plants, especially vegetables. By its deficiency diarrhoea is caused. Its important functions are - (a)      Along with c

Molecules that make possible active transport, Which are the molecules that...

Which are the molecules that make possible active transport through membranes? Ans) Active transport is made by particular membrane proteins. These proteins are known as "pumps"

What is the structure that maintains identical chromatids, What is the stru...

What is the structure that maintains identical chromatids bound? The structure that maintains identical chromatids bound is the centromere. Cell Nucleus Review - Image Diver

Explain le compte operation-rev procedure, Explain Le Compte Operation-REV ...

Explain Le Compte Operation-REV Procedure ? This is an alternative type of repair for TGA, VSD and left ventricular outflow obstruction (LVOTQ). In this operation an extrinsic

Define non-dietary treatment of burns, Define Non-Dietary Treatment of Burn...

Define Non-Dietary Treatment of Burns? While good nutritional care should be provided to the patient as soon as feasible it is equally imperative and at times critical to provi

Sickle Cell, Sickling occurs in deoxyhemoglobin S, but not in oxyhemoglobin...

Sickling occurs in deoxyhemoglobin S, but not in oxyhemoglobin S. Oxyhemoglobin has a small hydrophobic \"pocket\" in a ß chain region located in the interior of the protein. In de

Degradation of fats, Q. Explain Degradation of Fats? Fats are the este...

Q. Explain Degradation of Fats? Fats are the esters of glycerol and fatty acids and are called as 'glycerides'. Fats are susceptable to hydrolysis, oxidation and other che

Can pathophysiology causes cardiovascular disease, Q. Can Pathophysiology c...

Q. Can Pathophysiology causes cardiovascular disease? Cigarette use activates platelets, increases circulating fibrinogen, increases heart rate, and elevates blood pressure. It

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd