Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

Define the interaction of vitamin c with iron and cadmium, Define the inter...

Define the interaction of vitamin c with iron and cadmium? Toxic levels of dietary cadmium (5-200 ppm) interfere with iron absorption and produces iron deficiency. Supplements

Explain multidrug resistance, Multidrug Resistance  Treatment of MDRTB ...

Multidrug Resistance  Treatment of MDRTB is  based on limited data; patients should be referred, if possible, to clinicians who have experience in treating such cases. MDRTB (r

Illustrate disorders of the gastrointestinal tract, Q. Illustrate Disorders...

Q. Illustrate Disorders of the gastrointestinal tract? A host of disorders associated with a part or entire of the gastrointestinal tract. The important and the most frequent o

Hibernation and aestivation, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Hydropericardium-hepatitis syndrome, H yd r o p e r i c a r d i...

H yd r o p e r i c a r d i um - h e pa titi s syndrome Also known as 'Angara disease' or 'leachy heart disease', this complex illness of poultry spread rapid

Pain - criteria for endosteal implants, Q. Pain - criteria for endosteal im...

Q. Pain - criteria for endosteal implants? Pain and tenderness are subjective criteria and depend on patient's  interpretation of the degree of discomfort. Pain is defined as u

Latitudinal variations, Latitudinal Variations The latitudinal variatio...

Latitudinal Variations The latitudinal variation of temperature over the earth is the result of two main variables incoming solar radiation and the distribution of lan

Explain the direct microscopic counts (dmc), Explain the Direct Microscopic...

Explain the Direct Microscopic Counts (DMC) The process includes making a smear of food sample on a microscope slide followed by staining with an appropriate dye and viewing an

Medical therapy for tricuspid stenosis, Q. Medical therapy for tricuspid st...

Q. Medical therapy for tricuspid stenosis? Medical therapy is ineffective. With diuretics, symptoms of congestion are replaced by that of low cardiac output state. Occasional c

Keel, keel is the characteristic feature of flower of 1.tomato 2. tulip 3.u...

keel is the characteristic feature of flower of 1.tomato 2. tulip 3.ubdugifera 4.aloe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd