Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
Explain Procedure for Estimation of Sucrose by Fehling Soxhlet Method? Follow the proceedure enumerated herewith and carry out the experiment: 1. Standard titration (Standar
2. Mr Konarski, 73 years old, lives in a high-level care facility. He has dementia of the Alzheimer''s type, osteoarthritis in both knees and ankles, and hypertension, which is con
Explain mature monocot embryo:
Define drawbacks for underwater weighing method? Since the major drawbacks with UWW method are that the subject needs to be totally submerged underwater and exhale all of the a
Northern blot is a technique for analyzing mixtures of RNA by transfer of size-separated RNA fragments to a synthetic membrane, the presence and rough size of one particular type
It corresponds to the anterior and inferior interventricular grooves. Its right side is convex and bulges to the right ventricle. The septum has a posterior border which separates
The marine biomes consist of the earth's oceans and its associated areas like the shorelines, islands, reefs and estuaries. The marine waters contain about 3.5 percent salt, mostly
Q. Do echinoderms present external or internal fecundation? Is there sex division among individuals? The fecundation in echinoderms is external, gametes are liberated in water
What are passive and active immunization? According to the duration of the protection how do these types of immunization differ? Active immunization is that in which an antigen
Wheel organ of amphioxus.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd