Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
What is enzyme kinetics The study of the rate at which an enzyme works is called enzyme kinetics.
Explain the Atmospheric Dispersion Modeling 1. Dispersion model is a mathematical description of the meteorological transport and dispersion process that is quantified in terms
Explain the Liver Biopsy Assays? These are used to measure the total vitamin A stores, as well us, the response in levels of vitamin A relative to different dosages of the vita
Neuroglia - Organisation of Nervous System We may refer neuroglia as the connective tissue of the nervous system. It involves all elements of the nervous system other than ne
How would you design, in principle, an experiment to test the hypothesis that a particular variety of lettuce seed required daylight in order to germinate You would requi
What is Behavioural neurology Behavioural neurology contains the study of disorders of mood, personality, intelligence, perception and arousal and is concerned with the structu
Raised blood pressure is a major risk factor for cardiovascular disease. The higher the blood pressure, the higher the risk of stroke, coronary heart disease, kidney disease, heart
What are some of the things scientists may do to analyze data? They may use statistics to determine relation- ships among variables, compare the data with those obtained in ot
Explain some important phospholipids The chemical structure of phosphatidylcholine (lecithin) illustrated here in Figure is typical of the phosphatides found in the brai
What is the difference between taeniasis and cysticercosis? Taeniasis is the parasitic disease caused by the adult tapeworm installed within the human intestine. Cysticercos
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd