Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

Describe about the mitochondrial membranes, Describe about the mitochondria...

Describe about the mitochondrial membranes The composition of the two mitochondrial membranes is entirely different. The outer membrane is rich in lipids and contains nearly 50

Define changing body composition - age related functional, Define Changing ...

Define Changing Body Composition and Techniques for Measuring Body Composition? Changing body composition has been suggested as a pathway to explain age-related functional decl

Single vessel disease, Single Vessel Disease (SVD) :  They do well on medi...

Single Vessel Disease (SVD) :  They do well on medical treatment or with angioplasty. However if proximal LAD is significantly blocked and LIMA can be used as a conduit, surgery g

Emf, In a neuron with a resting potential of -65 mV, the distribution of wh...

In a neuron with a resting potential of -65 mV, the distribution of which ion across the neuronal membrane represents the greatest potential electromotive force (EMF)? A. Potassiu

Why nutrition is important for health, Why Nutrition is important for healt...

Why Nutrition is important for health Nutrition is one of the basic components of  life. It is an essential part of health care. You already know that good nutrition  is essent

Carbon, I need the parts of the structure carbon. Include elements,monomer...

I need the parts of the structure carbon. Include elements,monomer,polymer, and any sub units

What is the antagonism of the sympathetic, What is the antagonism between t...

What is the antagonism between the sympathetic and the parasympathetic neural actions? In general the actions of the sympathetic and the parasympathetic are antagonistic, i.e.,

What is erythroblastosis fetalis, It is a hemolytic disease, which makes in...

It is a hemolytic disease, which makes in newborn. Maternal IgG antibodies cross the placenta and destroy the red bleed cells. This develops when an Rh+ expresses an Rh antigen on

Explain about the intestinal calcium absorption, Explain about the Intestin...

Explain about the Intestinal calcium absorption? Intestinal calcium absorption is mainly controlled by the serum concentration of 1, 25- (OH) 2 D as discussed above. The activi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd