Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

What percentage of the offspring purple flowers have, Purple (P) flowers ar...

Purple (P) flowers are dominant and white (p) flowers are recessive. A homozygous dominant purple flower is crossed with a homozygous recessive white flower. what percentage of the

What are his morning and bedtime doses, a) Donald is a 50 year old male wit...

a) Donald is a 50 year old male with a long history of bipolar disorder. He has been hospitalized three times in the last 8 months for manic episodes. He was just admitted to your

Why body composition assessment is done in sports & exercise, Why Body comp...

Why Body composition assessment is done in sports and exercise? Body composition assessment is generally done in sports and exercise for the following reasons: 1) It can ser

Heliozoans - protozoan, Heliozoans - Protozoan Heliozoans are spherica...

Heliozoans - Protozoan Heliozoans are spherical protozoan that occur in the sea or in still bodies of fresh water. They are mainly located in the bottom debris. Fine needle li

Classification of carbohydrates - degree of polymerization, Define Classifi...

Define Classification of Carbohydrates - Degree of Polymerization? Classification on the basis of Degree of Polymerization (DP) Carbohydrates are classified by their degree

Biological functions in which chlorine ions participate, Q. What are the ma...

Q. What are the major biological functions in which chlorine ions participate? Like sodium cations, chlorine anions actively participate in the regulation of the osmolarity of

Explain the multiple organ dysfunction syndrome (mods), Explain the Multipl...

Explain the Multiple Organ Dysfunction Syndrome (MODS) Failure of essential organs is the most severe complication of sepsis and may result in death. The treatment of systemic

Thermal fluctuations and bonds, The stabilization energy of a bond or inter...

The stabilization energy of a bond or interatomic interaction is the change in energy upon breakage of a bond between two atoms (i.e., the change in energy when the atoms are moved

Show photosynthesis process in algae and plants, Q. Which all are the livin...

Q. Which all are the living beings that carry out photosynthesis? Which is the cell organelle responsible for the absorption of light for the photosynthesis process in algae and pl

Stabilisation - climax, Stabilisation - Climax The whole process of s...

Stabilisation - Climax The whole process of succession results in stabilisation of the vegetation which is now in complete harmony with the environmental complex of that plac

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd