Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

Explain solid salt and saturated aqueous solution, Explain solid salt and s...

Explain solid salt and saturated aqueous solution? In this example, the equilibrium system consists of crystalline PbCl 2 and an aqueous phase containing the species H 2 O, P

Regulation of respiration, Regulation of Respiration Whenever the need...

Regulation of Respiration Whenever the need for oxygen in the body increases, ventilation of the respiratory organs also increases. In the same way whenever the level of oxyge

Role of a nurse in legal psychiatry, ROLE OF A NURSE IN LEGAL PSYCHIATRY: ...

ROLE OF A NURSE IN LEGAL PSYCHIATRY: 1)   Standard Care : The nurse must function at laid down standards and keep up with the standard of  care by  knowing the policies  & pro

What are the symptoms and signs of pericardial effusion, Q. What are the Sy...

Q. What are the Symptoms and Signs of pericardial effusion? Asymptomatic: Slowly accumulating small to moderate pericardial effusion may not cause significant elevation of intr

Methods of estimation of energy expenditure and requirements, Define Method...

Define Methods of Estimation of Energy Expenditure and Requirements? There are a variety of methods available to measure human energy expenditure. Knowledge of these methods wi

Explain the food applications of locust bean gum, Food Applications of Locu...

Food Applications of Locust bean gum Locust bean gum has multiple applications in food industry.  Let us focus on a few of the important ones: Locust bean gum has been u

Explain the toxicity of vitamin e, Explain the Toxicity of Vitamin E? ...

Explain the Toxicity of Vitamin E? Vitamin E is relatively non-toxic. Adults tolerate doses as high as 100 to 1,000 IU per day. However, adverse effects such as muscle weaknes

Dietary modification, Q. Dietary modification? Dietary modification inc...

Q. Dietary modification? Dietary modification includes nutritional support that allows normal growth and development. Plasma phenylalanine concentration between the range of 40

Cardiomyocytes and motor neurons, Describe the mechanisms and genetic regul...

Describe the mechanisms and genetic regulations that underpin how two cells such as cardiomyocyte and a motor neuron have identical gnomes, yet are functionally and structurally di

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd