Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
Briefly explain Lichens organism? Lichens are unique organisms consisting of a symbiotic relationship between a fungus and a photosynthetic organism. They represent a type of s
Q. Applications of genetic engineering? Applications of genetic engineering in different fields are: • Agriculture: Crops having larger yields, drought -and disease -resis
Counseling Strategies The counseling strategies which may serve lo be useful are described herewith. Individual Counseling: Individual counseling is personal counseling. T
Explain the Psychological Management of eating disorders? All anorexia nervosa or bulimia nervosa patients are resistant to any kind of therapy and hospitalization may be a lif
Q. What are the functions of pantothenic and biotin acid for the body? How are these vitamins obtained? Biotin also known as vitamin B8 is a vitamin that acts in the metabolism
Q. Zonation in the ocean? The oceans play a major role in determining the climate and sustaining life on earth. Oceans help to redistribute the solar energy, through ocean curr
Define measuring body composition- Multiple isotope Dilution? Multiple isotope dilution (Total and extracellular body water): It is based on the principle that certain substanc
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
Which of the following statements least accurately explains our knowledge of how gene number relates to "organismal complexity"? A. Based on known gene numbers there appears to
Animals in the Community - Nature and Structure of Community The animals in the community are directly or indirectly dependent on the plants also time their activities in such
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd