Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
skeletal system of fish .exoskeleton and endoskeleton
3 benefits humans get from coral
What is the primary energy source for life on earth? The primary energy resource for life on earth is the sun. The sun plays the vital role of keeping the planet warmed and it
Explain how a cell produces and releases proteins. Proteins are made on ribosomes and packaged into vesicles by the Golgi apparatus. The vesicles move to the cell membrane and
Roan (RW) is a color of cattle in which both red and white hairs are present due to codominance. What are the phenotype and genotype ratios of offspring produced by: roan bull and
What are the soil-forming factors There are many degrees or variations of soil-forming factors, and the potential for creating different kinds of soil is enormous. However, whe
In an ecosystem various trophic levels are interlinked with each through a food chain. ( the transfer of food energy from green plants through a series of organism with repeated ea
Human lungs A pair of spongy, elastic and bag like structures of lungs is present in the chest cavity, one on either side of the heart. Lungs are enclosed by a double lay
Q. What is the ADP phosphorylation? What respectively are oxidative phosphorylation and photophosphorylation? ADP phosphorylation is the addition of one inorganic phosphate in
What is a graduated cylinder and how is it used? A graduated cylinder is a piece of laboratory glassware used to accurately calculate out volumes of chemicals for use in reacti
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd