Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

In which condition ulcer continues, Complete the sentence by choosing one o...

Complete the sentence by choosing one of the given biological terms. If an ulcer continues and ultimately erodes all the way through the wall of stomach or duodenum, condition

What do you understand by parapodia, What do you understand by Parapodia? ...

What do you understand by Parapodia? Paired lateral, unjointed appendages of polychaete worms. Parapodia have a variety of shapes, and their appearance is related to their nume

Ecosystem influence the biological diversity, Q. How does the vegetal strat...

Q. How does the vegetal stratification of an ecosystem influence the biological diversity? The vegetal stratification of the ecosystem like the strata of the Amazon Rainforest,

What is digestion, What is digestion? Digestion is the breaking down of...

What is digestion? Digestion is the breaking down of larger organic molecules obtained from the diet, e.g. carbohydrates, fats, proteins, into smaller ones, as glucose, fatty a

Explain citric acid cycle, Explain citric acid cycle In  the citric aci...

Explain citric acid cycle In  the citric acid cycle, the oxaloacetate is first condensed with acetyl CoA, and  then regenerated  as  the cycle  is  comp!eted.  But these  react

Explain the mechanism of temperature regulation, Any significant variation ...

Any significant variation in the internal temperature could have damaging effects on the body's enzymes. Homeotherms or warm-blooded animals including human can however generate co

Types of nursing units, TYPES OF NURSING UNITS: The design of nursing ...

TYPES OF NURSING UNITS: The design of nursing unit should maximize the function of the unit by following its task. The design of nursing unit has also under gone a radical cha

Explain what is mustard operation, Explain what is Mustard Operation ? ...

Explain what is Mustard Operation ? The difference in Mustard operation is that either pericardium or polyester patch is used for making the inter atrial baffle. The baffle com

Define traditional methods of food processing, Define Traditional Methods o...

Define Traditional Methods of Food Processing? Because food is so important to survival and most foods remain edible for only a brief period of time, people as the initial ages

Which compounds results from cyclization of glucose, Which of the following...

Which of the following compounds results from cyclization of glucose? Select one: a. alpha-D-glucopyronose b. beta-D-glucopyronose c. alpha-D-glucofuranose d. beta-D

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd