Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
Q. Can you explain Likar System with Electrodes? The 12 lead Mason - Likar system with electrodes is used. The plastic adhesive electrodes has self contained electrode jelly in
Q. How is cancer usually treated? If the cancer is in its initial stage cure is often done by surgical removal of the neoplastic tissue. The Cancers already disseminated are of
Explain how many bones in the human face? Ans) 14 bones are present in human face and these bones are known as sepio
Q. Describe the Basic Mechanisms in Plaque Formation? In experimental models and human disease, the first morphologic phenomenon observed in plaque formation is adhesion of mon
What is Cluster headache This is a unilateral pain in the head or face that rarely lasts longer than 2 hours but recurs repeatedly for a period of weeks or even months before d
The basic parts of RNA are the same than for DNA with two major differences. The pyrimidyne base uracil exchange thymine and ribose replace deoxyribose see the sugar, pyrimidines a
Define Derived proteins - Primary Protein Derivatives? Derived proteins are the derivatives of the protein molecule, apparently fonned through hydrolytic changes in the molecu
What is the first polar body? How different is it from the oocyte II? In oogenesis the oogonium differentiates into oocyte I (2n) and this cell enters meiosis. After finishing
How many parts of human body?
Tonicity and the plant cells. Complete the experiment and answer the questions. Table 7.3: Potato type: Potato A: Beginning displacement (ml) = Ending displacem
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd