Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
Define Age as a determinants of nutrient requirements? Age: Requirements change with increasing age between birth and maturity. Nutrient requirements per unit body weight are h
Q. Explain Spoilage of Soft Drinks? We all consume soft drinks, fruit juices, as well as, preserves, which normally are available in the market as tetrapacks, cans, bottles
Hermaphroditism You would have completely appreciated now that sexual reproduction is definitely the more advanced type of reproduction. It gives for combination of genes from
GOLGI APPARATUS Camillo Golgi in 1898 discovered a reticular structure in the cytoplasm of nerve cells with the help of metal impregnation technique using silver nitrate for whic
point out the criticism of lamarckism in any six short points.
Q. Can you briefly explain about parasitism? The Parasitism is an inharmonious interspecific ecological interaction in which individuals of a species (the parasites) explore or
Q. What are the various biological examples in which lysosomic enzymes play a fundamental role? The remodelation of the osseous tissue, the function of the elimination of the t
Name a few foods which are best blanched? Yes, green beans, carrots, okra, turnip and cabbage should always be blanched. On the other hand, blanching is not needed for onions,
A home-made wormery A wooden box 30 cm by 30 cm by 15 cm fitted with a glass front is beneficial for studying the habits of earth-worms. Fill the box nearly to the top with
Enumerate the important surgical anatomical structures in maxilla and the structures present in the posterior wall of the maxillary sinus. The important surgical anatomic struc
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd