Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

How are the concepts of the dna, Q How are the concepts of the DNA, gene, a...

Q How are the concepts of the DNA, gene, and proteins characteristics of living beings related? Characteristics of organisms depend on chemical reactions that take place in the

Autotomy and regeneration, Autotomy and Regeneration Shedding of body...

Autotomy and Regeneration Shedding of body parts in self-defense to avert the attention of the predator-enemy or in any other emergency is a type of autotomy (auto: self, tom

Nutrition, pictures for hetrotrophic and autotrophic mode of nutrition

pictures for hetrotrophic and autotrophic mode of nutrition

Explain the precautions for use of light microscope, Explain the Precaution...

Explain the Precautions for Use of Light Microscope? 1. Clean the lens system properly. 2. Use xylol only when necessary. 3. Never lower the body tube while looking throu

Evaporative cooling, Normal 0 false false false EN-IN ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Vascular lesions caused by leeches upon the blood vessels, Q. The vascular ...

Q. The vascular lesions caused by leeches upon the blood vessels of their host cause blood naturally to coagulate. How does the leech solve this problem since it could be expected

Zoonoses disease-chikungunya, Chikungunya Chikungunya, a metazoonosis,...

Chikungunya Chikungunya, a metazoonosis, is caused by chikungunya virus belonging to genus Alphavirus of the family Togavirideae. The virus is transmitted by infected mosquito

Living fossils, LIVING FOSSILS - 1. Limulus (Ki...

LIVING FOSSILS - 1. Limulus (King crab) Arthropoda 2 . Neopelina Mollusca 3 .

Uses of plural embryos, Uses of Plural Embryos Although the basic tha...

Uses of Plural Embryos Although the basic that trigger causes polyembryony are not fully understood, there has been no dearth of interest in exploiting supernumerary embryos.

Mitral valve, This valve has the same basic features as  of the Tricuspid V...

This valve has the same basic features as  of the Tricuspid Valve. It has an anterior and a posterior cusp. The anterior cusp is larger and is attached on the upper right part of t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd