Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

What is ascaris, What is ascaris? What is the disease caused by this worm? ...

What is ascaris? What is the disease caused by this worm? Ascaris, or Ascaris lumbricoides, is an animal of the nematode phylum, i.e., a roundworm. Ascaris causes ascariasis, a

Intravenous route, Intravenous Route This route of administering drug...

Intravenous Route This route of administering drugs is becoming more popular in the paediatric practice. Purpose of this Route When rapid action is required.

What are the most important structures in the nucleus, Q. What are the most...

Q. What are the most important structures within the cell nucleus? Within the cell nucleus the most important structures are the nucleolus, an optically dense region, spherical

Four characteristics of an efficient respiratory surface, State four charac...

State four characteristics of an efficient respiratory surface. Four characteristics of an well-organized respiratory surface are: a) thin epithelium, b) large surface ar

Briefly discuss the specialized organelles., The nucleus comprise the genet...

The nucleus comprise the genetic information of cell. In addition to activities which maintain the cell, nucleus creates copies of itself for new protozoans by either asexual repro

Reagent for separation of amino acid by paper chromatography, Define Reagen...

Define Reagent for Separation of Amino Acid by Paper Chromatography? The following reagents will be required for carrying out the experiment: 1. Amino acid solution- Weig

Explain the maximum containment laboratory, >>>>Write a short note on the f...

>>>>Write a short note on the following- Question 1 Centrism in Bioethics Question 2 Bio Safety check list Question 3 Enforcement of IPRs Question 4 T

Determine biologically compatibility of implant materials, Biologically com...

Biologically compatibility od implant materials An ideal implant material will elicit mainly physiological reactions within the surrounding tissues (bone, connective tissue and

Why red-green colour blindness is x-linked recessive, Red-green colour blin...

Red-green colour blindness is X-linked recessive. A woman with normal colour vision has a father who is red-green colour-blind. The woman has four sons, none of whom are colour-bli

Dna, What is a helix?

What is a helix?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd