Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
Aim : To prove that light is essential for photosynthesis. Apparatus : A potted plant, a light screen, beaker, iodine solution. Procedure : Keep a potted plant in darkness for tw
what is the fertilization?
Q. Describe the use of Sorbates acid in Microorganisms? Sorbic acid is an unsaturated carboxylic acid whose salts as sodium, calcium or potassium are used in foods upto
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
why their is different amount of yolk is present in eggs.
Energy Input in Ecosystem Survival and functioning of ecosystem is dependent on the input of energy. Continuous availability of energy is essential for supporting diverse ecos
Q. How many cellular nuclei does the pollen tube of angiosperms have? What is ploidy of each of these nuclei? The pollen tube explicitly the mature male gametophyte of angiospe
Define Protection against poisoning - Function of Vitamin E? 1) Protection against poisoning: It protects liver from injury due to carbon tetra chloride poisoning. 2) Pr
discrib mechanism of phloem transport
what is somogyi
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd