Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Radioactive Labelling
Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.
The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.
GENE INTERACTIONS (POST MENDELIAN GENETICS) Phenotypic expression of a gene may be altered by the influence of other genes. The modification of the normal phenotype of certain
How are the organelles of a single cell like the organs of a multicellular organism? Name two features of eukaryotic cells that prokaryotic cells lack.
Describe Examination of chest and abdominal ? As already mentioned earlier, any overt deformity of the chest, for example kyphosis, scoliosis, pectus exacavatum and pectus car
Action of Hormones We said earlier that hormones are released into the blood stream or extracellular fluid and therefore, reach most of the cells of the body. However, they
Habits of patients- Implants Smoking has been shown to predispose to poor bone quality by reduced vascularity. Therefore it is prudent not to accept heavy smokers as implant pa
Cytokinins - Apical Dominance Cytokinins are also involved in the regulation of apical dominance. Wickson and Thimann studied the interaction of cytokinins and auxins in contr
TRANQUILLIZER S - These are the drugs that reduce tension and anxiety, bring calmness without inducing sleep. (i) Phenothiazines - These are anti psychotic drugs.
Determine about the Accommodation of eye Accommodation is the ability of the overall refracting power of the eye to change to clearly view objects at different distances. This
Fast Response - Biological Responses Some of the fast responses are discussed below: It was found that when mung bean root tips were kept in a specific solution (contain
Speculate on the necessity of interdependence of volvox organisms in the colonial existence
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd