Radioactive labelling, Biology

Assignment Help:

Radioactive Labelling

Radioactive labelling method has been effectively applied on the chick blastoderm. The method includes labelling one embryo (donor) and grafting a part of it in similar position on another unlabelled embryo of similar stage (Host). An explanted blastodermis immersed in a medium consisting of the radioactive mtiated (H3) thymidine. In 3 to eight hours the tritiated thymidine is incorporated into the chromosomal DNA of dividing blastodermal cells. The embryo labelled in such type of a way by tritiated thymidine serves as a donor. Another embryo at similar stage of development as attained through the labelled donor in the meantime is then chosen to serve as the host. A small area of the host embryo is excised and replaced by a corresponding piece from the donor of which the fate is to be determined. Healing generally takes place quickly and the development is not impaired if the operation has been done carefully.

The thymidine does not pass out of the nuclei of the labelled cells but stays in the chromosomes of their descendents. Even though the radioactive thymidine present in the DNA is gradually diluted with each subsequent chromosomal replication radioactivity remains for a considerable time. Such type of composite embryo (partly from donor and partly from the host) is tested at a later stage of development for radioactivity through special techniques like autoradiography etc. Only the part(s) or structure(s) developed from the grafted piece display the presence of radioactivity thus establishing the fate of specific area taken from the donor.


Related Discussions:- Radioactive labelling

What is plant transpiration, What is plant transpiration? What are the two ...

What is plant transpiration? What are the two main types of plant transpiration process? Which of them is more significant in volume? Transpiration is the loss of water from th

Meosis, briefly describe the eggs and follicles

briefly describe the eggs and follicles

Echocardiography, Both global and regional systolic function are to be chec...

Both global and regional systolic function are to be checked. Global measures include ejection fraction, stroke volume, end systolic volume. (For measurement of systolic function u

Explain about the chemical carcinogens, Explain about the Chemical Carcinog...

Explain about the Chemical Carcinogens? Chemicals have been shown to be carcinogenic. Some are naturally occurring components of plants and microbial organisms. Some are synthe

What characteristics of fatty acids are false, What characteristics of fatt...

What characteristics of fatty acids are false? (Dissolves in water, used for energy storage, used to build lipids, found in triglycerides, found in steroids)?

EMBRYONIC PERIOD, LIST OUT THE DERIVATIVES OF 3 GERMS LAYER-ectoderm, meso...

LIST OUT THE DERIVATIVES OF 3 GERMS LAYER-ectoderm, mesoderm and endoderm

Describe the procedure of femoral approach, Q. Describe the procedure of fe...

Q. Describe the procedure of femoral approach? The catheter is inserted into the femoral sheath and advanced to the level of the left mainstem bronchus over the guidewire. Aft

Reproduction, The fusion of two individuals with each acting as a gamete is...

The fusion of two individuals with each acting as a gamete is known as

Bovine leukemia, B ov i n e leukemia Bovine leukemia, also known as...

B ov i n e leukemia Bovine leukemia, also known as bovine lymphosarcoma or leucosis, is a lymphoproliferative neoplastic disease of bovines. The virus belongs to genus Delt

Define principle of fehling test - reduction tests, Define Principle of feh...

Define Principle of fehling test - reduction tests? Sugars that possess a free or potentially free (those that can be converted to free) aldehyde or ketonic group have a proper

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd