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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Topology and Cell Organ Shapes Topology is an abstract mathematical exercise that deals with the possible shapes and surfaces of an endlessly/infinitely elastic body. Most cel
State about the lengthy administration of test battery The lengthy administration of test battery may be unsuitable for some individuals (such as demented or psychiatric patien
DIGESTION OF FOOD MATERIALS Digestion, you know, involves the mixing of food, its movement through the digestive tract and chemical breakdown of the large ntolecules of food
Q. What are the Signs of cardiac tamponade? The clinical presentation will be that of a low output state with anxiety, restlessness, dyspnoea, sweating, cold extremities and dr
Q. Is all the cartilage in the body the same? There are 3 types of cartilage in the human body: 1) hyaline cartilage; 2) elastic cartilage and 3) fibrocartilage. Hyaline cartil
The percentage of oxygen absorbed from the air in the lungs is always about the similar, so how can the oxygen supply to the blood be enhanced during vigorous activity? Breathi
Diffusion is the spreading of substance molecules from a region where the substance is more concentrated to other region where it is less concentrated. For example, during the boil
What is Cytogenetics? Before the advent of modern technology, the American biologists Thomas Hunt Morgan, G.W. Beadle, H. Sturtevant, Barbara McClintock, and others contributed
Define Recommended Intake of Fibre? 1. A minimum of fibre intake of 20 g/day is recommended by the American Dietetic Association (ADA), the National Cancer Institute, US and th
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