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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
What are cytochromes? Cytochromes are proteins of the internal mitochondrial membrane that are specialized in electron transfer and participate in the respiratory chain. Energi
Check Screws- Check screw access holes for closure. If screw access holes are uncovered, such as the bar-retained dentures, make sure that are free from debris. If screws can b
explain protozoa taxonomy
Determination of Neural Ectoderm by Induction Earlier you know that the dorsal mesoderm induces the ectoderm to differentiate into neural tissue. Spemann and his co-workers co
way of remember phylum protozoa
Pulses Careful examination of both upper and lower limb pulses is useful in detecting coarctation, and other arterial stenosis. The carotid arteries should be checked for sten
Q. Explain about Iceberg Phenomenon? Phenomenon means a fact or occurrence that appears. Let us see how iceberg phenomenon is applied to Diabetes Mellitus (DM). Cases of dia
A new drug named AGON-CaSR has been developed that is an agonist at calcium-binding sites of CaSRs (Calcium-Sensing Receptors) in plasma membranes of parathyroid gland cells.
Researchers (Helle et al., 2004) analyzed rates of twin births in the Sami population of Northern Scandinavia during the eighteenth and nineteenth centuries. They found that (1) a
How much salt is in the human body? 50 Kilo human has about 7 tablespoons of salt inside there body.
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