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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
All plants and animals have a natural microflora associated with them. For example, the plants have a natural microflora associated with the surface of roots, stems, leaves e
Q. List the stages of process of behaviour change? Stages of behaviour change process: · Unawareness · Awareness · Acquiring knowledge · Motivation · Trial
Q. What is Aprecise diagnosis? Aprecise diagnosis is necessary to establish the mode of therapy. Patients with initial blood phenylketonuria level of 121 m moll1 (> 2 mg/dl) sh
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Benthic Zone - Organisation of the Marine Ecosystem The benthic zone is divisible into sub zones horizontally. These are depicted in a cross section portion of the marine habi
Which of the following are true for ventilation? A. The problems with ventilation induced by injection of curare occur because of the drug's direct action on nicotinic ACh Rec
HACCP is a food-related operation to: A) Identify and assess hazard at every stage of operation, right from start to finish B) Determine the critical control points C) E
Q. What are the functions of the osseous tissue? The main functions of the osseous tissue that are to provide structural rigidity to the body and to delineate the spatial posit
Brasseler Endo Extractor tube Insert H file buccally and lingually and remove silver by friction.If I can't remove use solvent to desolve cement If the tip of silver not appear
what is its description and 3 most common example
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