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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
How are the heterotrophic beings divided in the ecological study of food interactions? The Heterotrophs are divided into consumers and decomposers. An ecosystem can survive wit
Graduate student Dee Innay works in a human genome lab, whose goal is to identify the human gene responsible for a form of muscular dystrophy (MD). Dee has mapped the MD gene to a
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In what forms are nitrogenous wastes excreted in birds, humans and aquatic turtles respectively ? Why so, describe?
Q. What is folic acid? Why is the anemia caused by deficiency of folic acid known as megaloblastic anemia? The folic acid when ionized it is called folate is a coenzyme that pa
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Explain the Lamarck's Theory in evolution? Prior to Darwin, most people believed in and held to the creationist viewpoint that species were created in their current forms, whic
Celia is a nurse in a geriatric ward. She noticed that older persons in her care are having problems sleeping at night. She decided to introduce non-pharmocologic ways of relaxat
How will the plant-incorporated protectants rules change EPA's current registration process? The rules clarify which plant-incorporated protectants are needed to be evaluated u
what is the scientific name of ameoba
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