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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Denitrification - Nutrient Cycles Nitrates are readily leached from the soil and also lost through denitrification the process by which molecular or gaseous nitrogen (N 2 ) as
Define Drug Effects on Food Intake - gastro intestinal tract? Affect the gastro intestinal tract: Gastrointestinal irritation and ulceration are serious problems with many drug
The initial stage of packaging have the binding of the chromosomal DNA to histones. Whole, in chromosomes the ratio of the DNA to histones on a weight basis is around 1:1. There a
Q. Merits and Demerits of system classification? Merits The merits of this system\are as under: • Careful observation and original description of the genera from living
Explain about the Maillard Reaction? The Maillard reaction sometimes called nonenzymatic, nonoxidative browning is simply the reaction between the amino group of a protein or
Define working of Body surface in excretion of zinc in human? Loss of zinc occurs due to the exfoliation of skin and sweating (0.7 -1.0 mg/day). Another route of zinc loss is h
The citric acid cycle also called as the TCA cycle (tricarboxylic acid cycle) and the Krebs cycle or the Szent-Györgyi-Krebs cycle is a series of chemical reactions used by all aer
Life cycle of malarial parasite When the mosquito sucks the blood, gametocytes enter its digestive system. They migrate into the walls of the digestive system and undergo
State the Continuous sutures Suturing technique It can be used to attach 2 surgical flap edges or to secure multiple interproximal papillae of one flap independently of the oth
Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following prevented him from being able to decl
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