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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
The North Island saddleback or tieke, Philesturnus rufusater, is an endemic New Zealand bird. Saddlebacks inhabit the middle or lower layers of the forest, and eat small invertebra
MULTIPLE ALLELES (i) More than two alternative forms (alleles) of a gene in a population occupying the same locus on a chromosome or its homologue are known as multiple
Accidents Resulting in Death Accidents that cause death are dealt with in a different way. The law requires full compensation to the widow for complete life or until remarriag
Cardiac Prophylactic antibiotics can decrease the incidence of infection after cardiac surgery, and intraoperative redosing has been associated with adecreased risk of postoper
A thiamine defiency will result in decreased activity of: -G-6 phosphase -transketolase -fructokinase
Annexin V is a protein that binds to Phosphatidylserine. Why is this useful in the lab? Does it have to do with testing whether the cell is in normal condition?
Exudate gums Most exudates gums come from compounds produced when the plant is wounded and these substances seal the wound. Most widely used exudate gum is 'gum Arabic' exuded
fish liver oil is rich in which vitamin ???
How have brain-imaging methods such as PET and MRI affected neuroscience research and clinical care? Techniques which capture pictures of the living human brain at work have be
Q. What are the symptoms of Rheumatic Heart Disease? Symptoms generally appear after 1 to 6 weeks of the fever and sometimes the infection may have been too mild to have been r
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