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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Give three examples of abiotic factors and explain how they interact. Abiotic factors contain temperature, humidity, pH, salinity, O 2 concentration, amount of sunlight, avail
Glycogen synthesis and degradation in the liver and muscle is very similar. Which of the following enzymes is not used in both muscle and liver for these metabolic pathways? -ph
What are the main proteins that constitute the sarcomere? What is the function of those molecules in the muscle cells? In the sarcomere there are organized myosin and actin blo
What is the action mechanism of the antiretroviral drugs called protease inhibitors which are used against HIV infection? Protease inhibitors are some of the antiretroviral dru
SQL's Fifth Concept - NULL Unfortunately, SQL embraces a fifth concept, called NULL, an apparently simple little thing but one that has pervasive effects on our usual underst
Slacklining is the act of walking across a suspended nylon strap tied between two vertical anchor points. List the senses used to maintain an upright position while slacklining an
Composition of oil gas is CH 4 = 25-30%; H 2 = 50-55%; CO = 10-12%; CO 2 = 3% It calorific value is 4500-5400 kcl/m 3 . It is used as laboratory gas. Oil gas is prepared b
Walking In the process of walking the motion of the two legs is coordinated so that one foot or the other is always in contact with ground. Walking is the best form of exercise
Locomotion Continuous formation of new pseudopodia keeps amoeba in constant locomotion .This is called amoeboid movement .It occurs in many other protozoans , in amoebo
Economic and ecological importance of protozoans
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