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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Attributes of Population The attributes of a population are of two basic types : i) Numerical attributes such as density, mortality (birth rate), mortality (death rate), disper
What is excretion? Excretion in Physiology is the method of elimination of metabolic wastes and other toxic substances from the body.
What is the Bio-medical waste The Bio-medical waste (Management & Handling Rules), 1998 is applicable to all persons, who generate, collect, receive, store, transport, treat,
Q. What is the main nitrogen waste of humans? Human beings excrete largely urea eliminated with the urine.
Leukotrienes and prostaglandins are produced only when the mast cell undergo degranulation and enzymatic break down of phospholipids in the plasma membrane. The effects formed b
microorganism
what are the gonad gland disorders and what do they do
You are working at a large, well funded research institute with the latest biochemical techniques available. The focus of your research is to find a specific mutation, or set of mu
Wind as a medium for Dispersal Many microorganisms, fungal spores and bacteria are freely transported over long distances even by moderate winds. In wind pollinated plants, the
History of diet nnd or tube feeding tolerance History of diet nnd or tube feeding tolerance: The dietitian presents the patient's history, which may include presence or hi
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