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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
DETERMIN A TIO N OF THE AGE OF FOSSIL - Radioactive clock (Boltwood -1907) - Half life of uranium is 4.5 billion years. This means half of total uranium disintegrates
Q. What do energy pyramids represent? The Energy pyramids represent the amount of accessible energy in each trophic level of the food chain.
The three charges in the are at the vertices of an isosceles triangle. Let q = 7.00 nC and calculate the electric potential at the midpoint of the base.
Mechanisms of different species of animal In an animal when the need to change the focal power of the eye arises, different mechanisms are there in different species of animal
What is crossing over? How is meiosis related to this phenomenon? The Linked alleles for instance, A-b and a- B form the gametes A-b and a-B that maintain the linkage of the al
Define Precautions for Determination of Inorganic Phosphorus? 1. All reagents should be added in the order mentioned. 2. Allow 10 minutes for the colour to develop in each t
basic difference beyween gymnosperm,angiosperm and pteridophytes.
What happens when the oxygen supply is insufficient to maintain aerobic cellular respiration during muscle exercise? If oxygen from hemoglobin or myoglobin is not enough for th
Class of Subphylum Uniramia - Symphyla Symphyla is yet other small myriapodous group that includes around 160 described species. These are also soil living forms and live in l
Explain the term Tracheostomy ? If the duration of mechanical ventilation is likely to be prolonged, it is wise to do an early tracheostomy. Otherwise, a tracheostomy is usuall
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