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Protoplasmic Theory
The living matter which forms the bodies of all living being is a special type of highly organized jelly like viscous semi fluid, and named Sarcode by Dujardin(1835) Later, then term protoplasm was used by purkinje (1840) for the living embryonic material of eggs and by Hugo von Mohl (1846) For the granular. Colloid, around the nucleus in a live cell. Protoplasm is so highly organized that it generates, Energy of Life i.e. it capable of converting chemical energy of matter into Biological energy. The is why Max Schultze (1861) called it the physical Basis of life .All this led O. Hertwig (1892) to propound his protoplasmic theory in which stated that all living matter, of which animals and plants are formed, is protoplasm.
What does it mean when a plasmid is cleaved with Hind III and Sca I? Does that mean that it opens up at those two locations and I have to insert whatever is complementary to it?
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