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Nucleic acid hybridization:
This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to the pathogen (target) by hybridization process. Recent advances in DNA cloning and sequencing have greatly increased the usefulness of this technique in the diagnosis and epidemiology of diseases. It is now possible to make DNA and RNA probes of high specificity using isotopic and non-isotopic labels. The specific cDNA probes have been used with advantage to diagnose a number of viral, bacterial and parasitic diseases obviating the need for the isolation of the causative agent. Post- mortem samples can also be used to identify the pathogen involved in a particular disease. The use of non-radioactive labeled photobiotinylated cold probes is more adaptive for field situations. Cold probes have been used in diseases like blue-tongue and FMD. DNA probes have also been used to detect FMD virus nucleic acid sequences persisting in the oesopharyngeal fluids of bovines when infectious virus can not be isolated. Thus, nucleic acid probes could even be used to screen the animals for persistent virus infections.
What are the typical vegetation and the typical fauna of the taigas? Taiga, or the boreal forest, is characterized by coniferous trees, pine forests. There are also mosses, lic
test to prove that carbondioxide is needed for photosynthesis to take place
Coronal Disassembly - If the existing restoration with marginal defect: - The crown was sectioned through the bucco-occlusaly , Christensen crown remover elevate and remove
Testicular Hypoplasia Hypoplasia refers to testicular disorders related to congenitally underdeveloped and underactive spermatic epithelium of one or both testes even though t
Explain some Physiological Changes during Pregnancy? A whole new life begins at conception. Organ systems develop rapidly and nutrition plays many supportive roles. Pregnancy f
Diaphragmatic Hernia: In this condition there is a slight herniation ofabdominal organs (stomach intestine and liver) or extreme protrusion of abdominal contents into the th
Some amount of energy is generatred from the oxidation of food materials is released as heat. This can be proved by germinating seeds. Aim: To show that heat is liberated during re
Are dogs colour blind
What is Cardiac Transplantation ? Cardiac Transplantation : The first successful clinical heart transplant was done by Christian Bmnard at Cape Town in South Africa in 196
what is mitosis and meiosis? what do they do?
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