Procedures for diagnosis - isolation of etiological agent, Biology

Assignment Help:

Isolation and identification of the etiological agent:

Isolation of the etiological agent is attempted in chicken embryos, cell cultures, laboratory animals, culture media or the host species. Cell cultures are most ideal, economic and often give quick results for virus agent. These could be homologous or heterologous; primary, secondary or established cell lines, and of diverse tissue origin (kidney, testes, liver, lung, whole embryo, etc.) depending upon the virus under study. If the viral agent involved is not known, primary kidney or thyroid cell cultures from embryo or young animals of the same (homologous) species should be preferred. The inoculums, after making it free from bacterial and fungal contamination by suitable antibiotic/antifungal treatment, are given 3 to 5 blind passages in a particular cell culture.

Type of cytopathic effects (CPE), demonstration of virus particles/ antigen in the infected cells, haemadsorption, haemagglutination (HA), haemagglutination inhibition (HI), neutralization of CPE with specific serum and physiochemical and biological properties may be studied for identification of the virus. When cell culture facilities are not available or the virus does not grow in any cell culture or produce CPE, chicken embryos may be inoculated in appropriate route i.e. CAM, AC, YS, amniotic cavity or intravenous. Laboratory animals viz. mice, guinea pig, hamster, rabbit, chicken, etc., which support the growth of a number of pathogens may also be used. Route of inoculation for these animals are selected in accordance with the agent being looked for. Strictly host specific viruses those do not grow in any cell culture or chicken embryos, the host species has to be used for its isolation.


Related Discussions:- Procedures for diagnosis - isolation of etiological agent

Endocrine interactions, Endocrine Interactions At the beginning of men...

Endocrine Interactions At the beginning of menstruation the inhibitory influence of the corpus luteum on the pituitary is removed and FSH is secreted in increasing amounts. Th

Explain about stacking faults, Explain about Stacking faults Stacking ...

Explain about Stacking faults Stacking faults happen in a number of crystal structures, but the common example is in close-packed structures.  Face-centered cubic (fcc) struct

Nutrient elements of plants, Nutrient Elements of Plants Let us first ...

Nutrient Elements of Plants Let us first find out the chemical composition of plants and see which element nature has selected to support their healthy growth. Only then we wi

Concerning their permeability how are membranes classified, Q. Concerning t...

Q. Concerning their permeability how are membranes classified? Membranes can be classified as permeable, impermeable, selectively permeable or semipermeable. An impermeable

Disorders associated with copulation and ejaculation, Disorders associated ...

Disorders associated with copulation and ejaculation Lack of libido refers to too long “reaction time” to complete absence of sexual desire for females in standing heat. The i

Synthetic Biology, I need analysis of Stza gene in Aspergillus nidulans thr...

I need analysis of Stza gene in Aspergillus nidulans through synthetic biology, OpenWetWare and Biobricks

Correction of reversible causes, In the long term reversible causes of hear...

In the long term reversible causes of heart failure like valvular lesions, myocardial ischemia, uncontrolled hypertension, arrhythmias, alcohol, negative inotropic agents, intracar

Explain the skeletal system, Explain the Skeletal System? Skeletal Sys...

Explain the Skeletal System? Skeletal System: The support framework, or skeleton, in the human body is composed of mostly hard mineral substances. Unlike crustaceans or insec

Ctenophora.., Ask queAffinities of ctenophora with platyhelminthesstion #Mi...

Ask queAffinities of ctenophora with platyhelminthesstion #Minimum 100 words accepted#

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd