Procedure for quantitative determination of viable microbes, Biology

Assignment Help:

Explain for Procedure Quantitative Determination of Viable Microbes?

Now carry out the exercise following the steps included herewith:

1. Label the diluent tubes from 10-1 through 10-7 and prepare dilutions.

2. Label the nutrient agar plates as 10-5, 10-6 and 10-7 in duplicate.

3. Pour 0.1 ml of each dilution from 10-5 through 10-7 over the agar surface of labeled plates in the centre.

4. Spread the inoculum over the entire surface by means of a sterile bent glass rod (spreader) till it is absorbed completely. Spreader can be made sterile by dipping in alcohol followed by passing through a flame of Bunsen burner or by autoclaving.

5. Keep the plates for incubation at 37°C for 24 hours.

6. Following incubation, observe and made the colony count. Calculate colony forming units/ml of the original sample by multiplying the number of colonies counted by the dilution factor and divided by the volume used. Use only dilution plates yielding colonies between 30 and 300 for calculations. Plates with more than 300 colonies are designated as too numerous to count (TNTC) and with fewer than 30 colonies are designated as too few to count (TFTC).


Related Discussions:- Procedure for quantitative determination of viable microbes

Circulatory system transports gases, Q. What are the three kinds of respira...

Q. What are the three kinds of respiration in which the circulatory system transports gases? The circulatory system has an important role in branchial respiration, cutaneous re

Which chemical causes tooth destruction, The bacteria that cause dental cav...

The bacteria that cause dental cavities in humans break down sugars, releasing what chemical which causes tooth destruction? a) Acids b) Bases c) Enzymes d) Monosaccha

What is diagnostic wax up, Diagnostic Wax Up A diagnostic wax up should...

Diagnostic Wax Up A diagnostic wax up should be done by you or got done from a laboratory for each and every case regardless of the extent or difficulty level of the case as it

Human impact on the environmetn, what are the two main pollutants that cont...

what are the two main pollutants that contribute to acidic rain?

Horse diseases-borna disease, Borna disease Borna disease is fatal neurolo...

Borna disease Borna disease is fatal neurological disease of horses and sheep caused by a virus (RNA) of the family Bornaviridae and the disease is characterized by sub acute meni

What is commensalism, What is commensalism? Commensalism is the ecologi...

What is commensalism? Commensalism is the ecological interaction in which one individual advantage while the other is neither benefited nor harmed. Commensalism is a harmonious

What is the difference among white and red blood cells, What is the differe...

What is the difference among white and red blood cells? What are leukocytes? Red blood cells are erythrocytes and white blood cells are the leukocytes. Leukocytes are cells

What is atomic constipation, Q. What is Atomic constipation? This type ...

Q. What is Atomic constipation? This type is most common; often it is called the "lazy .bowel". There is a loss of muscle tone causing weak peristalsis, the causes are: a) L

Disorder of skeleton and joints, DISORDER OF SKELETON AND JOINTS - 1 ...

DISORDER OF SKELETON AND JOINTS - 1 .      Sprain - It involves a stretching or tearing of tendons or ligaments. 2.      Arthritis - It is infalmation of joints.

What is bone grafting, What is Bone grafting The detailed description o...

What is Bone grafting The detailed description of the graft materials ranging from alloplastic, xenograft, allograft and autogenous grafts;  including their choice has been dis

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd