Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain for Procedure Quantitative Determination of Viable Microbes?
Now carry out the exercise following the steps included herewith:
1. Label the diluent tubes from 10-1 through 10-7 and prepare dilutions.
2. Label the nutrient agar plates as 10-5, 10-6 and 10-7 in duplicate.
3. Pour 0.1 ml of each dilution from 10-5 through 10-7 over the agar surface of labeled plates in the centre.
4. Spread the inoculum over the entire surface by means of a sterile bent glass rod (spreader) till it is absorbed completely. Spreader can be made sterile by dipping in alcohol followed by passing through a flame of Bunsen burner or by autoclaving.
5. Keep the plates for incubation at 37°C for 24 hours.
6. Following incubation, observe and made the colony count. Calculate colony forming units/ml of the original sample by multiplying the number of colonies counted by the dilution factor and divided by the volume used. Use only dilution plates yielding colonies between 30 and 300 for calculations. Plates with more than 300 colonies are designated as too numerous to count (TNTC) and with fewer than 30 colonies are designated as too few to count (TFTC).
Animal studies Most toxicological data for risk assessment are derived from the animal studies and it is, therefore, essential that these studies be conducted following widel
Differences between cells of Prokaryotes and Eukaryotes The prokaryotes also differ from the eukaryotes in many other ways, as you can see from Table. Table: Differences
Foods that are frequently incriminated in staphylococcal food poisoning include meat and meat products, poultry and egg products, egg, tuna, chicken, potato and macaroni, bakery pr
Explain Observation or Inference required for osazone test? 1. Crystals of different shapes will be seen. Glucose and fructose give needle shaped crystals and galactose gives f
Problem: Evaluation of disabilities due to occupational diseases (a) Describe the following terms: "impairment", "disability "and "handicap" (b) Show how the evaluation o
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
The man-made sources of air pollution are broadly categorized into three: Point or stationary sources: it includes such sources which pollute at particular. Pollutants from
Critical Thinking Why is it necessary to use the same restriction enzyme to cut two pieces of DNA that are to be joined together? As each restriction enzyme recognizes a specif
Lakes - Lentic Ecosystems Most lakes occur in regions which have recently been subjected to geological changes; say within the past 20,000 years. However, a few lakes, such a
Louis Pasteur (1860-1862) French): Even after the experiments of Spallanzani, the conflict of a biogenesis and biogenesis continued well into the middle of 19 th century till
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd