Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain for Procedure Quantitative Determination of Viable Microbes?
Now carry out the exercise following the steps included herewith:
1. Label the diluent tubes from 10-1 through 10-7 and prepare dilutions.
2. Label the nutrient agar plates as 10-5, 10-6 and 10-7 in duplicate.
3. Pour 0.1 ml of each dilution from 10-5 through 10-7 over the agar surface of labeled plates in the centre.
4. Spread the inoculum over the entire surface by means of a sterile bent glass rod (spreader) till it is absorbed completely. Spreader can be made sterile by dipping in alcohol followed by passing through a flame of Bunsen burner or by autoclaving.
5. Keep the plates for incubation at 37°C for 24 hours.
6. Following incubation, observe and made the colony count. Calculate colony forming units/ml of the original sample by multiplying the number of colonies counted by the dilution factor and divided by the volume used. Use only dilution plates yielding colonies between 30 and 300 for calculations. Plates with more than 300 colonies are designated as too numerous to count (TNTC) and with fewer than 30 colonies are designated as too few to count (TFTC).
At 1 AM, an impermeable membrane separates a 1 liter solution of 1M NaCl in the left compartment from a 1 liter solution containing both 1M NaCl and 1M KCl in the right compartment
phase of alpha taxonomy
M i croinjection method: In another approach fertilised eggs are microinjected by DNA. Microinjection requires excess of fertilized eggs. Gene transfer by microinjection is
Which of the following best explains the difference among dominant and recessive alleles? A. The recessive allele encodes a protein with normal activity whereas the dominant al
What is Tricuspid Atresia: Surgery for Single Ventricle Physiology In tricuspid atresia, the right atrium fails to open into right ventricle through a right atrioventricular va
Classification of Phytoestrogens? Phytoestrogens can be divided into 3 main classes: - Isoflavones, - Coumestans and - Lignans. All are diphenolic compounds with st
Secondary Growth in a Typical Dicotyledonous Stem In stem, the secondary growth in thickness in diameter is confined both intrastelar, i.e., in the stele and extrastelar regio
Q. How does the inflammation mechanism work? When some tissue injury other vasoactive and occurs histamine substances (called mediators of inflammation) are released, they caus
Explain the Bio availability of Vitamin A? By now it is clear that vitamin A is supplied in two forms. One form is retinol, from animal foods such as liver, fatty fish, eggs, a
Define the Magnesium Requirements in body? Let us learn about the magnesium requirements. Since plant foods are particularly high in magnesium, on a vegetarian diet with plenty
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd