Procedure for quantitative determination of viable microbes, Biology

Assignment Help:

Explain for Procedure Quantitative Determination of Viable Microbes?

Now carry out the exercise following the steps included herewith:

1. Label the diluent tubes from 10-1 through 10-7 and prepare dilutions.

2. Label the nutrient agar plates as 10-5, 10-6 and 10-7 in duplicate.

3. Pour 0.1 ml of each dilution from 10-5 through 10-7 over the agar surface of labeled plates in the centre.

4. Spread the inoculum over the entire surface by means of a sterile bent glass rod (spreader) till it is absorbed completely. Spreader can be made sterile by dipping in alcohol followed by passing through a flame of Bunsen burner or by autoclaving.

5. Keep the plates for incubation at 37°C for 24 hours.

6. Following incubation, observe and made the colony count. Calculate colony forming units/ml of the original sample by multiplying the number of colonies counted by the dilution factor and divided by the volume used. Use only dilution plates yielding colonies between 30 and 300 for calculations. Plates with more than 300 colonies are designated as too numerous to count (TNTC) and with fewer than 30 colonies are designated as too few to count (TFTC).


Related Discussions:- Procedure for quantitative determination of viable microbes

Examples of invertebrate nervous system, Q. Using examples of invertebrate ...

Q. Using examples of invertebrate nervous systems how can the process of evolutionary cephalization be described? Considering the example of invertebrates it is observed that e

Determine the function of osseointegration, Determine the function of Osseo...

Determine the function of Osseointegration Osseointegration is also a measure of implant stability, which occurs at two stages- primary and secondary. Primary stability has be

Definition of osseointegration in microscopic biophysical, Q. Definition of...

Q. Definition of Osseointegration in microscopic biophysical? Osseointegration implies that at light microscopic and electron microscopic levels, the identifiable components of

How to calculate the raw score for each scale, How to calculate the raw sco...

How to calculate the raw score for each scale The raw score for each scale is the sum of the 0, 1 and 2 item scores. Thus, the higher the score, the poorer the performance. Th

Solid or liquid at room temperature, do fats with one or more 'kinky' tail ...

do fats with one or more 'kinky' tail fatty acids tend to be solid or liquid at room temperature? These are found in triglycerides forming what? Solid fats or oils? Is it opposite

How are the heterotrophic beings divided, How are the heterotrophic beings ...

How are the heterotrophic beings divided in the ecological study of food interactions? The Heterotrophs are divided into consumers and decomposers. An ecosystem can survive wit

Anatomical coordinate systems, Brain anatomical coordinate systems are the ...

Brain anatomical coordinate systems are the first potential point of confusion, as there are different ones due to the fact that research is conducted in different species where d

Which term explains flow characteristic of blood, Which of the below terms ...

Which of the below terms is used to explain the study of factors which influence flow characteristics of blood as it moves through the body? Is it: a) Anginology b) Hemodyna

What is the function of the right ventricle, What is the function of the ri...

What is the function of the right ventricle? To where does the right ventricle pump the venous blood? The function of the right ventricle is to get venous blood from the right

What is hmp pathway, What is  HMP pathway The HMP pathway like glyco...

What is  HMP pathway The HMP pathway like glycolysis occurs in the cytosol of the cell. However, C02  which is not produced  in glycolysis, is a characteristic product in HMP

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd