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Explain Procedure for Spore Staining in a Given Bacterial Culture
Carry out the exercise following the steps enumerated herewith
1. Take a clean, non-greasy slide. Prepare bacterial smear by taking a small amount of culture and mixing it with a drop of water on a slide. Spread it in a rounded manner.
2. Air dry and heat fix the smear.
3. Keep the slide on a beaker having boiling water. Flood the smear with malachite green and allow the steam to pass through it for 4-5 minutes. Replenish the stain if it starts evaporating and drying.
4. Remove the slide from the beaker and allow it to cool.
5. Decolourize the smear by washing slowly under running tap water.
6. Counter stain the smear with safranin for 30 seconds.
7. Wash the slide again with tap water.
8. Blot dry the slide with blotting paper and observe under oil immersion.
What is HMP pathway? Give any two points of its significance The HMP is an alternate oxidative pathway for the metabolism of glucose. The significance of the pathway is that
Q. What is Pulmonary Edema? When the capillary pressure exceeds the plasma osmotic pressure, fluid first accumulates in the interstitial spaces. The components of the interstit
You ran both a body temperature and room temperature test for each enzyme you tested. Did the two temperatures seem to have an effect on the enzyme Exercise? Why or why not?
Explain the diagnostic set-up surgical stent The patient should understand the procedure and be warned of any complications. They should have agreed with the treatment plan, t
REPRODUCTIV E ISOLATION - According to the biological species concept, members of one species are reproductively isolated from members of all other species. Accordingly, re
Why do preserved foods not spoil? Plant and animal cells must stay in an isotonic, or neutral, solution to survive. When salt or sugar is added, more of the cells wither and di
Gene Project
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basis of genetic transplantation
Polyarthritis 1) Gonococcal - Therapeutic trial of pencillin may help in diagnosis of gonococcal infection. 2) Viral infections such as rubella and hepatitis B may have
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