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Explain Procedure for Spore Staining in a Given Bacterial Culture
Carry out the exercise following the steps enumerated herewith
1. Take a clean, non-greasy slide. Prepare bacterial smear by taking a small amount of culture and mixing it with a drop of water on a slide. Spread it in a rounded manner.
2. Air dry and heat fix the smear.
3. Keep the slide on a beaker having boiling water. Flood the smear with malachite green and allow the steam to pass through it for 4-5 minutes. Replenish the stain if it starts evaporating and drying.
4. Remove the slide from the beaker and allow it to cool.
5. Decolourize the smear by washing slowly under running tap water.
6. Counter stain the smear with safranin for 30 seconds.
7. Wash the slide again with tap water.
8. Blot dry the slide with blotting paper and observe under oil immersion.
Determine the term - coupling factors The coupling factors, also called the F l ATPase, present on the inner mitochondrial membrane facing the matrix side, are a very complex a
Q. How do taenias obtain food and make gas exchange? The Tapeworms have sucking and hooks structures on their heads (scolex) that fixate the parasite in the gut wall these stru
what are the characteristics of algae?
Explain Electron Microscope? Electron microscopes have higher magnification and resolving power than light microscopes. What do we mean by magnification and resolving power? W
Define Functions of sodium and chloride? So far you have learnt that most minerals participate in important functions of body as they support the activity of specific enzymes.
Q. What is Salty taste? Various ions both cations and anions are responsible for the salty taste. These include: K (Potassium), Na (Sodium), Li (Lithium), Cl, Br (Bromine), I (
Why is it important for core body temperature to stay more constant than skin temperature?
Viscosity and Plasticity of colloidal particle Various degrees of viscosity and plasticity are encountered in colloids. Viscosity may be described as resistance to pouring.
DNA damage caused by ultraviolet light: 1. Cyclobutane-type pyrimidine dimer is the major photoproduct formed 2. A second product, 6-4 photoproduct, is formed in about 10 % of UV i
Protein Glycosylation is the reaction in that a carbohydrate, example for a glycosyl donor that is attached to a hydroxyl or other functional set of another molecule a glycosyl acc
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