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Explain Procedure for Spore Staining in a Given Bacterial Culture
Carry out the exercise following the steps enumerated herewith
1. Take a clean, non-greasy slide. Prepare bacterial smear by taking a small amount of culture and mixing it with a drop of water on a slide. Spread it in a rounded manner.
2. Air dry and heat fix the smear.
3. Keep the slide on a beaker having boiling water. Flood the smear with malachite green and allow the steam to pass through it for 4-5 minutes. Replenish the stain if it starts evaporating and drying.
4. Remove the slide from the beaker and allow it to cool.
5. Decolourize the smear by washing slowly under running tap water.
6. Counter stain the smear with safranin for 30 seconds.
7. Wash the slide again with tap water.
8. Blot dry the slide with blotting paper and observe under oil immersion.
POST-OPERATIVE CARE OF PATIENTS The first few days following cardiac operations are the most critical in terms of the patient's survival. The safety with which a patient can
Which of the following statements concerning the inheritance of a dominant trait is true? Every affected person must have at least one affected parent. The trait is observed to ski
Define Erythropoietin A. acts by stimulating the production of red blood cells by the peritubular interstitial cells of the kidney cortex. B. is secreted by cells in the bon
A fruit fly has four pairs of chromosomes in its cells. At meiosis, how many dissimilar combinations of maternal and paternal chromosomes are possible in the gametes? In gamet
Fidelity - Synthetic Characters Fidelity refers to the degree to which a species is restricted in its occurrence to a particular kind of community. The species with low fideli
How potassium is essential for the following physiological functions It is vital for photosynthesis. When K + ion is deficient, photosynthesis declines and respiration incr
BRONCHOSCOPY: Bronchoscopy is the passage of lighted bronchoscope into the bronchial tree. Bronchoscopy may be performed with rigid steel of flexible fiberoptic instruments.
Estimate the molar mass of protein? An aqueous solution of the protein bovine serum albumin, containing 2:00 *10 -2 g of protein per cubic centimeter, has an osmotic pressure
Aujeszky's disease This is primarily a disease of pigs, which serve as the principal reservoir host. The secondary hosts include cattle, horse, sheep, goat, dog and cat but hu
habitat, habit, cell wall chemistry, reason why cell wall like this of Archaebacteria , eubacteria,fungi,algae,bryophytes ,pteridophyes, gymnosperms , angiosperms..
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