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Explain Procedure for Spore Staining in a Given Bacterial Culture
Carry out the exercise following the steps enumerated herewith
1. Take a clean, non-greasy slide. Prepare bacterial smear by taking a small amount of culture and mixing it with a drop of water on a slide. Spread it in a rounded manner.
2. Air dry and heat fix the smear.
3. Keep the slide on a beaker having boiling water. Flood the smear with malachite green and allow the steam to pass through it for 4-5 minutes. Replenish the stain if it starts evaporating and drying.
4. Remove the slide from the beaker and allow it to cool.
5. Decolourize the smear by washing slowly under running tap water.
6. Counter stain the smear with safranin for 30 seconds.
7. Wash the slide again with tap water.
8. Blot dry the slide with blotting paper and observe under oil immersion.
Explain the Gall bladder disease - Effect of Obesity? Obesity is one of the risk factors for formation of gallstones. The super saturation of bile with cholesterol in obese ind
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Importance Value Index (IVI) - Synthetic Characters In any study of community, the quantitative value of each of the frequency density, and cover has its own importance. But t
Types of individuals in Cnidaria There are two forms of individuals in Cnidaria: Polyp Medusa Polyp is tubular, the oral end being free carrying
Q. Explain Bruce Protocol? Bicycle Ergometry Most bicycle test are performed sitting upright, but supine bicycle test have become more popular. Treadmill Test The
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steps involved in glycolysis
Minerals and Trace Elements Oxygen, carbon, hydrogen and nitrogen are the most common elements that make up 96% of the total weight of a mammal. The next most abundant element
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