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Explain Procedure for Spore Staining in a Given Bacterial Culture
Carry out the exercise following the steps enumerated herewith
1. Take a clean, non-greasy slide. Prepare bacterial smear by taking a small amount of culture and mixing it with a drop of water on a slide. Spread it in a rounded manner.
2. Air dry and heat fix the smear.
3. Keep the slide on a beaker having boiling water. Flood the smear with malachite green and allow the steam to pass through it for 4-5 minutes. Replenish the stain if it starts evaporating and drying.
4. Remove the slide from the beaker and allow it to cool.
5. Decolourize the smear by washing slowly under running tap water.
6. Counter stain the smear with safranin for 30 seconds.
7. Wash the slide again with tap water.
8. Blot dry the slide with blotting paper and observe under oil immersion.
Specific Gravity The density of a substance is defined as mass per unit volume. The density of a substance is a characteristic property and has a definite value at a given te
Explain why it is essential that muscle cells, during anaerobic respiration, convert pyruvic acid (pyruvate) into lactic acid, even though the conversion results in an accumulation
Q. What are the periods of the second meiotic division? Meiosis II is divided into metaphase II, prophase II, anaphase II and telofase II.
Q. Is there an exchange of cells between the mother and the fetus through the placenta? Under usual conditions there is no passage of cells across the placenta during gestation
Restaurant deep fat frying evaluation A number of factors are studied when evaluating frying oils. During deep fat frying, the fat is exposed continuously to elevated temperat
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PULSE Pulse frequency and heart rate are identical i.e. 72. It can be felt in superficial arteries as in wrist (radial artery [thumb] and ulner artery of brachial art.),
How can the enzymes be classified? Explain giving examples. Based on structure, enzymes can be classified into monomeric enzymes and oligomeric compounds. Monomeric enzym
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Surplus red blood cells, required to meet an emergency, are generally stored in what organ of the human body? Is it the: a) Liver b) Spleen c) pancreas d) Kidneys
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