Procedure for preparation of selective & differential medium, Biology

Assignment Help:

Procedure for Preparation of Selective and Differential Medium

Now prepare the MacConkey agar medium following the steps given herewith:

1. Weigh and dissolve all ingredients except agar one by one in 500 ml of distilled water.

2. Make the volume to 1000 ml with distilled water and set the pH to 7.3.

3. Add agar and dissolve it by heating.

4. Autoclave the medium at 15 psi for 15-20 minutes.

5. After cooling to 45oC, dispense the medium aseptically into sterile petri plates.

6. Allow to solidify and then inoculate with the given cultures.

For inoculation, divide the plate into different sections by marking the bottom of the dish. Label each section with the name of the organism to be inoculated. Inoculate the plate with different organisms by making a single line of inoculation of each organism at appropriate section/place as shown in margin Figure.

7. Incubate the plates in inverted position for 24 hours at 37oC and then record observations.

 


Related Discussions:- Procedure for preparation of selective & differential medium

Illustrate the applications of vitamin B6, Illustrate the Applications of v...

Illustrate the Applications of vitamin B 6 The heating processes employed in the industry for the sterilization of milk based formulations will greatly reduce their vitamin B

Etiological factor of malabsorption syndrome, Q. Etiological factor of mala...

Q. Etiological factor of malabsorption syndrome? The causes cited for malabsorption can be associated with a number of diseases. Many of these diseases you may not know presen

Therapeutic dose of pyridoxine, Q. Therapeutic dose of pyridoxine? Ther...

Q. Therapeutic dose of pyridoxine? Therapeutic dose of pyridoxine (lg/day) is usually tried in all patients with hypermethioninernia and homocystinemia. For patients who do not

Differance among subphylum chaetognatha and urochordata, Explain differance...

Explain differance between Subphylum Chaetognatha and Subphylum Urochordata? Subphylum Chaetognatha: Larval stages of this small group share some of the characteristics with

Procedures for diagnosis - epidemiological data , Procedures for diagnosis ...

Procedures for diagnosis Epidemiological data:   The epidemiological information with regard to the history, symptoms, morbidity, mortality, seasonality, age, sex, breed and spe

Systolic dysfunction onset of symptoms, Q. Systolic dysfunction onset of sy...

Q. Systolic dysfunction onset of symptoms? In asymptomatic patients with normal LV function, the rate of progression to symptoms and/or LV Systolic dysfunction is about 4 per c

Explain the cultural practices of single crop, Explain the Cultural Practic...

Explain the Cultural Practices of single crop Continuous cultivation of a single crop over years on the same site causes accumulation of a particular group of microbes, which d

Citrate levels would be low in the cytosol, When the ratio of insulin to gl...

When the ratio of insulin to glucagon is >1, which is the following is true with regards to fatty acid synthesis? -malonyl CoA will inhibit carnitine palmitoyl transferase 1

Poultry and duck diseases-avian encephalomyelitis, Avian encephalomyelitis ...

Avian encephalomyelitis This is an acute infectious disease of birds (3 weeks old), which is caused by a member of the genus Enterovirus in the family Picornaviridae. Epid

Types of cells in phloem, Types of cells in Phloem The phloem consists...

Types of cells in Phloem The phloem consists of 4 types of cells: i).sieve elements, ii) companion cells, iii) phloem parenchyma and sometimes iv) phloem fibres. The metabolit

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd