Procedure for preparation of selective & differential medium, Biology

Assignment Help:

Procedure for Preparation of Selective and Differential Medium

Now prepare the MacConkey agar medium following the steps given herewith:

1. Weigh and dissolve all ingredients except agar one by one in 500 ml of distilled water.

2. Make the volume to 1000 ml with distilled water and set the pH to 7.3.

3. Add agar and dissolve it by heating.

4. Autoclave the medium at 15 psi for 15-20 minutes.

5. After cooling to 45oC, dispense the medium aseptically into sterile petri plates.

6. Allow to solidify and then inoculate with the given cultures.

For inoculation, divide the plate into different sections by marking the bottom of the dish. Label each section with the name of the organism to be inoculated. Inoculate the plate with different organisms by making a single line of inoculation of each organism at appropriate section/place as shown in margin Figure.

7. Incubate the plates in inverted position for 24 hours at 37oC and then record observations.

 


Related Discussions:- Procedure for preparation of selective & differential medium

Class turbellaria, plz answer me how I arrange my topic with its order ?

plz answer me how I arrange my topic with its order ?

Describe the phylogenetic system of classification, Q. Describe the phyloge...

Q. Describe the phylogenetic system of classification? 1) Hutchinson believed in Monophyletic origin of Angiosperms from a hypothetical group, which he' named, as Proangiosperm

What do you determine by mantle, What do you determine by Mantle? A thi...

What do you determine by Mantle? A thin sheetlike membranous extension of visceral mass of molluscs which forms two flaps of skin. Mantle secretes the shell on the dorsal side

Can you illustrate animal and plant cells, Q. What is the name of the cytop...

Q. What is the name of the cytoplasm division in the end of mitosis and what are the differences in this process between animal and plant cells? Cytoplasm division occurs afte

Complications of making a definite classification, Assume for this question...

Assume for this question that we are discussing a rare human disorder. Describe as detailed as possible the characteristics of this disorder if it is: autosomal dominant autosomal

Define body mass index (bmi), Define Body Mass Index (BMI)? Weight for ...

Define Body Mass Index (BMI)? Weight for height as a marker of nutritional status has the advantage that it is independent of other factors that affect foetal outcome such as m

Define altered glucose metabolism - nutrition during stress, Define Altered...

Define Altered Glucose Metabolism - Nutrition during Stress? Blood glucose levels are generally elevated in the infected patient but plasma insulin levels are normal or even hi

Two homologous human chromosomes, Two homologous human chromosomes have the...

Two homologous human chromosomes have the following structure: --A--B-.-C--D--E--F--G--H--I--J-- --A--D--C-.-B--E--F--G--J--I--H-- where the letters represent genetic markers and t

Explain glucose molecule runs the krebs cycle twice, Why can it be said tha...

Why can it be said that each glucose molecule runs the Krebs cycle twice? Every glucose molecule "cycles" the Krebs cycle twice because after glycolysis each used glucose has g

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd