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Define Principle of colorimetric method-2, 4 dinitrophenylhydrazine?
Ascorbic acid is oxidized to dehydro ascorbic acid by copper (Cu++) ions. 2, 4 dinitrophenyl hydrazine reacts with the ketone group of dehydro ascorbic acid under acidic conditions to give osazone. The depth of the yellowish orange coloured osazone produced upon acidification with sulphuric acid is directly proportional to the amount of ascorbic acid present and can be measured photometrically at 540 nm wavelength.
As you carry out the procedure in the laboratory you will understand the method even better. So then let's get started and practically learn the two methods we have studied above. There are four activities in this practical.
Define needs of Vitamins arid Minerals in Postoperative Nutritional Care? Vitamins are required for wound healing after a surgery. Vitamin C provides the cementing material of
Explain the Stages In Taxonomic Procedures? Divided into three levels or phases: 1) Alpha (α) phase, 2) Beta (β) phase, 3) Gamma (γ) phase. Alpha Taxonomy The
Q. How do antibodies work to neutralize antigens? The immunoglobulins or antibodies act to facilitate the destruction of antigens: they attract phagocytic leukocytes, they dire
Food Applications of Carrageenan Carrageenan consists of a family of hydrocolloids, which have different properties and it has a wide variety of uses. Some examples of properti
Autogenic and Allogenic Succession In many cases the living beings of an ecosystem, modify their environment considerably by their growth, death and decay. The changed condit
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Define Interactions between Volatile Substances and Proteins? Flavour binding may involve adsorption at the surface of food or penetration to the food interiority by diffusion
Hydrostatic Skeleton The functioning of the hydrostatic skeleton in an animal depends upon the musculature being arranged around an enclosed volume of fluid. After that, cont
Substances which are insoluble in a membrane are carried across membrane with concentration gradient by means of a carrier molecule in a process termed as: a) Diffusion. b)
According to the chemistry concept the existence of different kinds of crystal of the same chemical compound is known as polymorphism
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