Principle of colorimetric method-2, 4 dinitrophenylhydrazine, Biology

Assignment Help:

Define Principle of colorimetric method-2, 4 dinitrophenylhydrazine?

Ascorbic acid is oxidized to dehydro ascorbic acid by copper (Cu++) ions. 2, 4 dinitrophenyl hydrazine reacts with the ketone group of dehydro ascorbic acid under acidic conditions to give osazone. The depth of the yellowish orange coloured osazone produced upon acidification with sulphuric acid is directly proportional to the amount of ascorbic acid present and can be measured photometrically at 540 nm wavelength.

As you carry out the procedure in the laboratory you will understand the method even better. So then let's get started and practically learn the two methods we have studied above. There are four activities in this practical.


Related Discussions:- Principle of colorimetric method-2, 4 dinitrophenylhydrazine

What is heterophagic intracellular digestion, Heterophagic intracellular di...

Heterophagic intracellular digestion is the breaking into minute substances of external substances engulfed in the cell by pinocytosis or phagocytosis. Phagosomes or pinosomes fuse

Cryptosporidiosis, Cryptosporidiosis Cryptosporidiosis, a protozoan diseas...

Cryptosporidiosis Cryptosporidiosis, a protozoan disease of the intestinal tract, is caused by Cryptosporidium parvum and Cryptosporidium muris. It infects man, dog, cat, calves,

Define exclusion chromatography - basic separation technique, Define Exclus...

Define Exclusion chromatography - basic separation technique? It is a chromatographic process, in which separation of the sample components takes place according to the molecul

Define changing trends in dietary intake, Define Changing Trends in Dietary...

Define Changing Trends in Dietary Intake? As a practicing dietician, you must be sensitive to the society as a whole. The fat requirements have been worked out with a premise o

Respiration, what is the meaning of direct and indirect respiration

what is the meaning of direct and indirect respiration

Define economic evaluation of malnutrition, Define Economic Evaluation of M...

Define Economic Evaluation of Malnutrition? You must be familiar with a proverb frequently used in the field of economics, which says - "resources are limited and wants are unl

Inhibition of microorganisms in food, Q. Inhibition of microorganisms in fo...

Q. Inhibition of microorganisms in food? Microorganisms use our food supply as a source of nutrients and energy. They increase their numbers by utilizing nutrients. This can re

Cost-benefit relationship regarding sewage treatment, Q. What is the cost-b...

Q. What is the cost-benefit relationship regarding sewage treatment as a strategy to fight water pollution? To treat sewage is greatly cheaper for society. The non-treated sewa

What essential function do serve, Why do membranes figure so prominently in...

Why do membranes figure so prominently in eukaryotic cells? What essential function do they serve?

How is digestion performed in protozoans, How is digestion performed in pro...

How is digestion performed in protozoans? Digestion in protozoans is intracellular digestion: organic material is internalized and degraded inside the cell. Protozoans get

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd