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Define Preparation of Dilutions - Standard or Total Plate Counts?
1. Serial dilution of homogenized sample in an appropriate diluent.
Preparation of dilutions - Decimal dilutions are prepared in a diluent [peptone water (1%) and 0.5% NaCl] and some dilutions are plated. Selection of the dilutions to be plated is made on the basis of expected microbial load in the sample.
2. Plating on to suitable agar medium by pour plate or surface plating.
3. Incubation of plates at appropriate temperature (usually 35-37°C) for a given time (24-48 hours).
4. Counting of all visible colonies manually or using a Quebec or electronic counter.
5. Calculating CFU/ml or CFU/gm of the food sample.
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Pyruvate Dehydrogenase Complex The Pyruvate Dehydrogenase Complex (PDC) is one of the single central enzymes of aerobic metabolism. After completion of this animation you shoul
what is the function of cornified layer of the skin
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Q. What is the concept of universality of the genetic code? What are the exceptions to this universality? The genetic code is universal because the rules of protein codificatio
Centrifugal Pump: This is available for clinical perfusion from 1976 (Bio Medicus Pump). It is disposable, causes less blood trauma and reduces the risk of massive air embolism.
Define Precautions for Acid Fast Staining? 1. Do not allow carbol fuchsin to dry on heating. 2. Maintain aseptic conditions. 3. Decolourization should be done till no red
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Self-Pollination - Types of Pollination Self-pollination refers to the transfer of pollen grains from the anther to the stigma of the same flower. In chasmogamous flowers the
Types of Ovules Anatropous This is the most common type of ovule in angiosperms in which the ovule undergoes curvature such that the micropylar end comes to lie parallel
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