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Preparation - Culture Medium
Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.
Determine the Fransworth 100 Hue Panel Test This is a simple and useful test using colour chips arranged in a particular sequence. Colour deficient patients make errors in arra
ALCOHOLISM - Continual heavy consuming of alcohol is called alcoholism or drinking . Word alcohol is refered to C 2 H 5 OH or ethyl alcohol or ethanol or grain alcohol.
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