Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

How does self infection by tapeworms occur, How does self infection by tape...

How does self infection by tapeworms occur? Taeniasis patients might be develop the most severe form of the worm infection, cysticercosis, because their feces have eggs and pre

Describe about the halstead''s discriminating tests, Describe about the Hal...

Describe about the Halstead's discriminating tests Halstead's discriminating tests are viewed as a measure of adaptive abilities, of skills that ensured man's survival on the p

Deficiency diseases-parturient paresis , Parturient paresis (milk  fever, h...

Parturient paresis (milk  fever, hypocalcaemia) Parturient paresis is an acute to peracute non-febrile disease, which occurs in diary cows and buffaloes usually around the t

Explain adverse effects of abacavir, Adverse effects of Abacavir  In 3-...

Adverse effects of Abacavir  In 3-9% of patients, a severe hypersensitivity reaction, usually with fever and sometimes with respiratory or gastrointestinal symptoms, malaise an

Psychosocial problems, PSYCHOSOCIA L PROBLEMS  - 1 .       Accidental...

PSYCHOSOCIA L PROBLEMS  - 1 .       Accidental injury - Automobile accidents are no. 1 killers of adolescents, as they drive often fast. 2 .       Depression - I

Explain increased fibrinogen levels-thrombogenic factors, Explain Increased...

Explain Increased Fibrinogen Levels and Other Thrombogenic Factors ? Thsombogenesis is an important component in the pathological process of atherosclerosis and so it is not s

What are some factors that can lead to protein denaturizing, Protein denatu...

Protein denaturizing can be source by temperature variation, pH change, and changes in the concentration of surrounding solutes and by other processes. Most proteins are denatured

What are coacervates, What are coacervates? Coarcervates are small stru...

What are coacervates? Coarcervates are small structures made of the aggregation of organic molecules under water solution. By electrical attraction the molecules join into bigg

Explain carboxypeptidase, Carboxypeptidase The  inactive  zymogen  proc...

Carboxypeptidase The  inactive  zymogen  procarboxypeptidase  is  activated  by  trypsin .The further action on the polypeptides is carried out by carboxypeptidase which  attac

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd