Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Explain the histologic techniques, Explain the histologic techniques A...

Explain the histologic techniques A clinician has to base the diagnosis only on the clinical and radiographic findings as microbiologic and histologic techniques are restricte

Observing where soil bacteria live, Observing where soil bacteria live ...

Observing where soil bacteria live Dig up a clover, alfalfa or soy-bean plant. Carefully increase all the soil from the roots and see if you can find the little white nodules o

Define methods of quantitative analysis and colorimetry, Define Methods of ...

Define Methods of Quantitative Analysis and Colorimetry? Many methods of quantitative analysis are based upon the production of coloured solutions in such a way that the intens

Groups of living beings that form the protist kingdom, Q. Which are the gro...

Q. Which are the groups of living beings that form the protist kingdom? The protist kingdom includes algae and protozoans. (Two groups of fungi with similar characteristics to

What is the virus that causes flu, What is the virus that causes flu? Why d...

What is the virus that causes flu? Why doesn't the body create permanent immunity against that virus? How does the vaccine against flu work? Flu is a disease caused by the inf

Removal of impurities in water, Many common methods are employed for the re...

Many common methods are employed for the removal of impurities from water. Some of them are: Sedimentation Filtration Reverse osmosis Electro dialysis Aeration

Sample titration for determination of iodine number of lipid, Define Sample...

Define Sample titration for Determination of the Iodine Number of Lipids? With a clean dry pipette, accurately measure 0.3 mL of oil into a clean and dry iodination flask (Dupl

Difference between adrenergic and cholinergic fibre, Difference between Adr...

Difference between Adrenergic and Cholinergic Fibre- S.NO .   1. ADRENERGI C FIBRE   Release nor adrenaline at their CHOLINE

What is bile salts, What is Bile salts Bile salts have a considerable...

What is Bile salts Bile salts have a considerable ability to lower surface tension. By  this property, they prevent  the coming together of the small fat droplets in  the  in

What is the mechanism described by darwin, What is the mechanism described ...

What is the mechanism described by Darwin that eliminates species less adapted to environmental conditions? The mentioned mechanism is the natural selection.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd