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Preparation - Culture Medium
Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.
How do placental mammals reproduce? Placental mammals reproduce sexually, they have internal fecundation and they are viviparous, i.e., their embryo creates within the mother's
Subphylum Mandibulata Most of them have three pairs of walking legs, mandibles, compound eyes, antennas, some with wings. There are four classes. Crustacea - aquatic with
Describe the Positive End Expiratory Pressure (PEEP)? A positive pressure may be applied at the end of the expiratory phase during mechanical ventilation, instead of allowing t
i want about superclass pisces
Needlestick/sharp instrument injuries are of major concern to health care workers. All of the significant exposures reported in the academic year 2003/04 in our Faculty, were injur
characterstics and classification of vertebrates
Are there ribosomes in a plant cell? Yes, in fact there are ribosomes in both plant and animal cells. That is right. Ribosomes are the site of synthesis of polypeptide chain
Why is it necessary to have the second division in meiosis even though cells have already been reduced to haploid number after the first division?
Q. What is coronary disease? The Coronary disease, or the coronary insufficiency, is a disease in which there is total or partial obstruction of one or more of the arteries tha
what do primary tissues of a plant do
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