Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Determine the implant insertion, Implant insertion The technique for in...

Implant insertion The technique for insertion of the implant depends largely upon the system being used. In general, the final bone preparation site diameter is slightly smalle

Define the term - dna polymerase, Which of the following best describes the...

Which of the following best describes the reasons why the sliding clamp is able to confer processivity on DNA polymerase and not DNA primase? A. The sliding clamp coats the tem

Main means by which autotrophic beings obtain energy, What is the main mean...

What is the main means by which autotrophic beings obtain energy? The major means by which autotrophs obtain energy is photosynthesis (There are also chemosynthetic autotrophs)

Temperature - estuaries, Temperature - Estuaries Another importan...

Temperature - Estuaries Another important variable is temperature. The temperature of estuary keeps on fluctuating it heats up and cools down more rapidly under prevailin

Blackquarter, B l ac k q u ar t e r The disease is otherwise k...

B l ac k q u ar t e r The disease is otherwise known as black leg or emphysematous gangrene, and it is associated with severe toxaemia, emphysematous swelling of muscl

What is osseointegration in macroscopic biomechanical, Q. What is Osseointe...

Q. What is Osseointegration in macroscopic biomechanical? A fixture is osseointegrated if there is no progressive relative motion between the fixture and surrounding living bon

Illustrate about sarcoplasmic reticulum membranes, An increase in the calci...

An increase in the calcium conductance of all sarcoplasmic reticulum membranes of a skeletal muscle with no external forces on it leads to A. Increased binding of calcium ions

Animal versus plant metabolism, Which of the following BEST characterizes g...

Which of the following BEST characterizes gas exchange in animal versus plant metabolism: Animals take in O2 and release CO2. Plants take in and release both O2 and CO2. Both anima

Define the microbiological study of water, Define the Microbiological Study...

Define the Microbiological Study of Water? Water is a common carrier of infectious diseases. Even clean and clear water which looks pure may be contaminated with pathogenic mic

Define factors that affecting the bmr, Define Factors that Affecting the BM...

Define Factors that Affecting the BMR? Basal metabolic rate, we know, is the largest component of the daily energy demand representing 45 to 70 percent of daily total energy ex

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd