Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

What are the inorganic substances, What are the processes that autotrophic ...

What are the processes that autotrophic beings use to produce organic material from inorganic substances? Autotrophic beings make organic material by photosynthesis or by chemo

What is the phase of the menstrual cycle, Q. In general what is the phase o...

Q. In general what is the phase of the menstrual cycle when copulation may lead to fecundation? Although this is not a rule, to be effective fecundation in general must occur w

Name three characteristics of mitochondria, Name three characteristics of m...

Name three characteristics of mitochondria and chloroplasts that support the endosymbiotic hypothesis of eukaryotic evolution. Both organelles replicate independently of the ce

Explain about the micronutrient deficiencies, Explain about the Micronutrie...

Explain about the Micronutrient Deficiencies? Micronutrient deficiencies are more common during calamities. Can you guess, why? This is due to the lack of diversified food item

Explain the history of animal taxonomy, Explain the History of Animal Taxon...

Explain the History of Animal Taxonomy? The beginning of animal taxonomy was made by Aristotle (384-322 B.C.). He extensively studied about anatomy, embryology, habit and ecolo

What is the significance of protein for the cell, Q. How does the sodium-po...

Q. How does the sodium-potassium pump present in the cell membrane work? What is the significance of this protein for the cell? The sodium-potassium pump is the transport prote

After the blastula stage what is the next stage, Q. After the blastula stag...

Q. After the blastula stage what is the following stage of the embryonic development? What is the passage from blastula to the next stage called? The blastula turns into gastru

Explain the natural history of coronary artery diseases, Explain the NATURA...

Explain the NATURAL HISTORY OF CORONARY ARTERY DISEASES (GAD)? The natural history of CAD is very important from the preventive point or view. Though the usual manifestations o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd