Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Determine requirement of trace elements for infant, Determine Requirement o...

Determine Requirement of Trace elements for Infant? Most elements, such as zinc and iodine, hold a major significance in infant nutrition. Zinc is associated with growth while

Which hormone influence maturation of the ovarian follicles, Q. After mense...

Q. After menses what is the hormone that influences the maturation of the ovarian follicles? The maturation of the ovarian follicles after menses is stimulated by the action of

What is the prevention of perinatal transmission, Prevention of perinatal t...

Prevention of perinatal transmission  Zidovudine alone, started at 14-34 weeks of gestation and continued in the infant for the first 6 weeks of life, reduced HIV transmission

What is diverticular disease, Q. What is Diverticular Disease? A common...

Q. What is Diverticular Disease? A common disorder of the large bowel, diverticulosis, is an early stage of the disease. It can be identified in 15% of the people over the age

Can you define musculoskeletal system, Q. Which are the organs that are par...

Q. Which are the organs that are part of the musculoskeletal system? The key tissues and organs that are part of the musculoskeletal system in humans are the cartilages, the mu

Which are the phases of the menstrual cycle, Which are the phases of the me...

Which are the phases of the menstrual cycle? The menstrual cycle is separated into two main phases: the follicular (or menstrual) phase and the luteal (or secretory) phase.

Growing roots from different parts of plants, Growing roots from different ...

Growing roots from different parts of plants Secure a box of sand and place it away from direct sunlight. Wet the sand thoroughly and keep it moist. Plant the following thi

Explain process of glycosylation, Glycosylation: The covalent addition of ...

Glycosylation: The covalent addition of the sugar moities to N or O atoms present in the side chains of various amino acids of certain proteins, usually occuring within the Golgi

Diversified animal group of the planet, Q. Which arthropod class is the mai...

Q. Which arthropod class is the mainly diversified animal group of the planet? How can this evolutionary success be explained? The insects are the animal group with most divers

Campylobacter infection, C ampylobacter infection C a mpylobacter ...

C ampylobacter infection C a mpylobacter jejuni is the commonest species found in poultry. These are delicate organisms that may not survive outside the host unless prot

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd