Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Define the objectives in the biochemistry of the eye, Define the objectives...

Define the objectives in the biochemistry of the eye. After going by this section, you should be familiar with: a. Dynamic of vascular supply of eye; b. The cornea metabo

Determine the placental mammalian embryos, Which is the extraembryonic memb...

Which is the extraembryonic membrane whose function is to store nitrogen wastes of the embryo? Is this function present in placental mammalian embryos? The allantois is the ext

Explain the activity of decomposers, Why are producers the first trophic le...

Why are producers the first trophic level to advantage from the activity of decomposers? Decomposers return nutrients in dead tissues and wastes to the soil or water; producers

RNA synthesis, Distinguish features in the mechanisms of RNA synthesis, + (...

Distinguish features in the mechanisms of RNA synthesis, + (RNA), (-) RNA, and ds RNA viruses.

Osmoregulatory animal, Osmoregulatory Animal An osmoregulatory animal ...

Osmoregulatory Animal An osmoregulatory animal is generally in an osmotic steady state even though there may be hourly and daily variations in osmotic balance. The concentrati

Explain the oligosaccharides (dp: 3-9) - carbohydrates, Explain the Oligosa...

Explain the Oligosaccharides (DP: 3-9) - carbohydrates? Oligosaccharides consists of short chains of monosaccharide units joined by covalent bonds. The glycosidic bond may be α

What are the positions of myosin and actin molecules, Q. What are the posit...

Q. What are the positions of myosin and actin molecules in the sarcomere before and during the muscle contraction? Schematically actin filaments attached perpendicularly to bot

What is the group of the phylum chordata, What is the group of the phylum C...

What is the group of the phylum Chordata that first colonized the terrestrial environment? From which habitat did they come? Amphibians, partially aquatic partially terrestrial

Cardiac cycle, Cardiac Cycle Cardiac cycle has two phases-systole and...

Cardiac Cycle Cardiac cycle has two phases-systole and diastole. Ventricular systole and diastole occur as a result of depolarization and chamber volume and pressure. The di

Explain adverse effects of lactic acidosis, Adverse effects of Lactic acido...

Adverse effects of Lactic acidosis- Lactic acidosis and severe hepatomegaly with steatosis have been reported with use of other nucleoside analogs. The most common adverse effe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd