Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

What do you understand by melanocytes, Q. What are melanocytes? Melanoc...

Q. What are melanocytes? Melanocytes are epithelial cells of the skin specialized in secretion of melanin and Melanin is a pigment that besides coloring the iris of the eye and

Define about the absorption of iron, Define about the Absorption of Iron? ...

Define about the Absorption of Iron? Before it can be absorbed, iron whether it is in the form of haem or non-haem must be released from the food matrices where it is bond with

Neurotransmitters, Neurotransmitters We know that transmission of sign...

Neurotransmitters We know that transmission of signals from nerves to muscles is affected by acetylcholine a transmitter substance. Similarly neuron-neuron transmission is als

Define synthesis of hormones - vitamin c, Define Synthesis of Hormones - Vi...

Define Synthesis of Hormones - Vitamin C? Ascorbate is involved in the amidation, thereby conferring stability to hormones such as thyrotropin releasing hormone, adrenocorticot

Are nematodes triploblastic or diploblastic animals, Q. Are nematodes tripl...

Q. Are nematodes triploblastic or diploblastic animals? Just like platyhelminthes, nematodes are triploblastics that is they present three germ layers (ectoderm, endoderm and m

Centrifugal pump-type of blood pump, Centrifugal Pump: This is available ...

Centrifugal Pump: This is available for clinical perfusion from 1976 (Bio Medicus Pump). It is disposable, causes less blood trauma and reduces the risk of massive air embolism.

Determine about the open head injuries, Open Head Injuries Open head in...

Open Head Injuries Open head injuries are TBIs in which the skull is penetrated, as in gun shot or missile wounds, or in which fragment of bone penetrate the brain substance. O

Evaluate the magnitude of st depression, Q. Evaluate the Magnitude of ST De...

Q. Evaluate the Magnitude of ST Depression? It is intuitive that the magnitude of ST depression should correlate with the degree of the ischaemia. In patients with left main or

Striped muscles, STRIPED MUSCLES - These muscles are found in the li...

STRIPED MUSCLES - These muscles are found in the limbs, body walls, tongue, pharynx and beginning of oesophagus and are under the control of animal's will. These muscle f

Explain what is dna, Explain what is DNA? DNA : DNA is the source of t...

Explain what is DNA? DNA : DNA is the source of the information that directs the development of the cell. Although all segments of DNA are not active in every cell in the huma

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd