Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Explain the dna hybridisation, Explain the DNA Hybridisation? This tech...

Explain the DNA Hybridisation? This technique is used to study or compare the genetic affinity between two organism. In such studies the deoxyribonucleic acid (DNA) of both org

Intermediate filaments - role of cytoskeleton structures, Intermediate fila...

Intermediate filaments - Role of Cytoskeleton Structures These filaments are intermediate in size among microtubules and microfilaments and are 10 nm in diameter. Five classes

Define field test used to assess aerobic capacity, Define Field Test used t...

Define Field Test used to Assess Aerobic Capacity? A field test that has been used extensively in nutrition studies to assess aerobic capacity measures Heart Rate responses to

What characteristics of fatty acids are false, What characteristics of fatt...

What characteristics of fatty acids are false? (Dissolves in water, used for energy storage, used to build lipids, found in triglycerides, found in steroids)?

Explain cognitive - behavioural approaches, Cognitive - Behavioural Approac...

Cognitive - Behavioural Approaches They includepsychoeducatiorzal and rational-emotive  therapy. The goal is to identify problem behaviour and irrational beliefs and then to de

How different is the amphibian heart from the fish heart, Q How different i...

Q How different is the amphibian heart from the fish heart? The fish heart has only two chambers, a ventricle and an atrium, and the blood that comes to it is purely venous.

Enumerate the principles of suturing, Enumerate the principles of suturing ...

Enumerate the principles of suturing Principles of Suturing are as follows: - Sutures should always be inserted through the more mobile tissue flap first. - A circular fo

Change of health status over time, Change of Health Status Over Time Hea...

Change of Health Status Over Time Health of a nation can be gauged from the available information on death.Disaggregated data by causes of death is more reflective of the status

Supply of animals in lab, Supply of Animals: Lab animals must be obtained ...

Supply of Animals: Lab animals must be obtained from accredited dealers and by accredited dealers, we mean suppliers in the business of supplying animals for lab use, and not from

Fiehes test and aniline chloride test, Q. Fiehes test and Aniline chloride ...

Q. Fiehes test and Aniline chloride test? Determine the adulteration in the given honey sample by Fiehe's test and Aniline chloride test This activity will help you to: •

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd