Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

What is the importance of surface area, A. How is the villus the functional...

A. How is the villus the functional unit of the small intestine? In your answer, include a discussion of its structure and function and the enzymes found there. What is the importa

Explain strengthening the public health measures - anaemia, Explain Strengt...

Explain Strengthening the public health measures? Parasitic infestations and protozoal infections cause iron deficiency through loss of blood or destruction of red blood cells.

The process of glycolysis produces, In the presence of oxygen, the process ...

In the presence of oxygen, the process of glycolysis produces which of the following products? A) 2 glycerol B) 1 lactate C) 2 lactate D) 2 pyruvate E) 1 pyruvate

What are some functions of the epithelium, What are some functions of the e...

What are some functions of the epithelium? The epithelial tissues can do covering, impermeability and protection against the environment, for instance, in the skin, resorption

How do bacteria reproduce, How do bacteria reproduce? Bacteria replicat...

How do bacteria reproduce? Bacteria replicate by binary fission (scissiparity). Some bacteria though present a kind of sexual reproduction (transformation, transduction or conj

Explain zidovudine, Zidovudine Zidovudine is available alone and in fix...

Zidovudine Zidovudine is available alone and in fixed-dose combinations with lamivudine as Combivir and with lamivudine and abacavir as Trizivir. It can be given in combination

What is the digestive enzyme that acts within the stomach, What is the dige...

What is the digestive enzyme that acts within the stomach? Which type of food does it digest? What are the cells that produce that enzyme? The digestive enzyme that acts in the

Deficiency diseases-metabolic disorders, Metabolic Disorders The disea...

Metabolic Disorders The disease conditions, which are attributable to an imbalance between rates of input of dietary nutrients and the output of production, are defined as pro

Define terpene alcohols - non glyceride fractions, Define Terpene Alcohols ...

Define Terpene Alcohols - non glyceride fractions? Partly free, partly esterified e.g. ferulic acid; these are found in significant amounts in rice bran oil, wheal germ oil, so

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd