Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

AMOEBA, mORPHOLOGY OF AMOEBA AND ITS ADAPTATIONS

mORPHOLOGY OF AMOEBA AND ITS ADAPTATIONS

Chlorocruorins - respiratory pigments, Chlorocruorins - Respiratory Pigment...

Chlorocruorins - Respiratory Pigments These types of respiratory pigments are found in four polychaete families: Sabellidae, Serpulidae, Ampharetidae and Chlorhaemidae. The pi

How much time is required for a single cell, How much time is required for ...

How much time is required for a single cell of Detoxivicatium completeium to grown into a population large enough to fill the volume of the earth?

Haccp control measure, HACCP Control Measure HACCP Control Measure : ...

HACCP Control Measure HACCP Control Measure :  Any action or activity that can be used to prevent, eliminate or reduce a significant hazard.

What does an anther have on it, What does an anther have on it? The ant...

What does an anther have on it? The anther is the part of the flower that keeps the pollen. This and the filament both make up the stamen.

Explain the term ferns, Explain the term Ferns? Ferns are plants that...

Explain the term Ferns? Ferns are plants that have vessels that conduct water, minerals, and nutrients, and reproduce through spores, rather than seeds. Ferns are therefore o

Explain what is inversion, Explain what is Inversion ? Inversion is ano...

Explain what is Inversion ? Inversion is another way in which genetic recombination can occur, as seen below. In this case, the change occurs within a chromosome, rather than b

Procedure for spore staining in a given bacterial culture, Explain Procedur...

Explain Procedure for Spore Staining in a Given Bacterial Culture Carry out the exercise following the steps enumerated herewith 1. Take a clean, non-greasy slide. Prepare b

Similar organs and similar general shape be explained, How can the fact tha...

How can the fact that fishes and dolphins have similar organs and similar general shape be explained? Dolphins and Fishes have similar organs and shape because although they ha

Wbcs types, mode of action of wbcs types

mode of action of wbcs types

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd