Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Basic planes of cleavage, Basic Planes of Cleavage The basic planes a...

Basic Planes of Cleavage The basic planes along which the egg and its daughter blastomeres are divided during early cleavage are: A. Meridional Plane - the cleavag

Compound leaf, Compound leaf is the leaf in which the blade forms small le...

Compound leaf is the leaf in which the blade forms small leaflets. Compound leaves which have several small leaflets originating from the central axis are termed as pinnately comp

Explain spontaneous closure of defects in details, Explain Spontaneous Clos...

Explain Spontaneous Closure of Defects in details? Some defects have a tendency towards spontaneous closure and this can influence the timing of intervention. The defects known

Photochemical stage of the photosynthesis process, Q. What are the procedur...

Q. What are the procedures of the photochemical stage of the photosynthesis process? Photolysis of water, with liberation of molecular photophosphorylation and oxygen of ADP, w

Why is genetic homozygosity dangerous, Why is genetic homozygosity dangerou...

Why is genetic homozygosity dangerous to a nearly extinct species? Genetic homozygosity leaves no variation for natural selection to act on. Thus, a new disease could wipe

Dna vs protein, Describe the difference between dna and protein. (structura...

Describe the difference between dna and protein. (structural unit, linkage bond, primary and spatial structure)

Explain the process of fatty acid elongation, The process of fatty acid elo...

The process of fatty acid elongation is similar to fatty acid synthesis as it also requires both: -malonyl CoA and NADPH -malonyl CoA and NADH -Acetyl CoA and NADH

Pericardial valves: carpentier edwards, Pericardial Valves: Carpentier Edwa...

Pericardial Valves: Carpentier Edwards (Perimount Valve) :  Pericardium preserved in gluteraldehyde and mounted on flexible stent is available for mitral and aortic positions

State the aim of neuropsychological assessment, State the aim of Neuropsych...

State the aim of Neuropsychological assessment Neuropsychological assessments aim at specifying in as much detail as possible the functional deficits that exists in a manner th

Determine the structural classification of bone, Structural classification ...

Structural classification of bone Macroscopically, bone tissue can be classified into two types, compact and trabecular bone. Compact bone is dense, corticated and makes up the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd