Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Functions of colour in foods, Q. Functions of Colour in Foods? Let us n...

Q. Functions of Colour in Foods? Let us now look at the different functions of colour in details, which are enumerated herewith: 1. The maturity of many fruits and vegetable

Why enzymes work to speed up a chemical reaction, Enzymes work to speed up ...

Enzymes work to speed up a chemical reaction by: a. attaching coenzymes to the substrate b. heating up the substrate c. lowering the activation energy of the reaction d. supplyi

Microscope, How to calculate the magnifying power of a microscope i

How to calculate the magnifying power of a microscope i

Genomic clone, Genomic clone is the piece of a DNA taken from genome of a ...

Genomic clone is the piece of a DNA taken from genome of a cell or an animal, and spliced into the bacteriophage or other kind of cloning vector. A genomic clone might contain cod

Describe how potassium is essential, How potassium is essential for the fol...

How potassium is essential for the following physiological functions It is vital for photosynthesis. When K + ion is deficient, photosynthesis declines and respiration incr

Refining of crude oil, The crude oil obtained from earth's crust is refined...

The crude oil obtained from earth's crust is refined by following steps. Step 1: separation of water Cottrell's process is used for separation of water. Crude oil is an emu

Predict the genotypes and phenotypes of the f2, In rats, the gene pigment (...

In rats, the gene pigment (P) is dominant to no pigment (p). The gene for black (B) is dominant to the gene for cream (b). If a pigment gene (P) is absent, genes B and b are inoper

What is coarctation of aorta, What is Coarctation of Aorta ? More commo...

What is Coarctation of Aorta ? More common in males (3:l). Narrowing of aorta typically located near aortic attachment of ligamenturn arteriosum or PDA. It can be a localized

Which terrestrial vertebrate group is extremely rare deserts, Which terrest...

Which terrestrial vertebrate group is extremely rare in deserts? The Amphibians are terrestrial vertebrates very rare in desert environments (although there are a few species a

Theca - body form protozoans, Theca - body form Protozoans It is a clo...

Theca - body form Protozoans It is a close-fitting hard structure around the plasma membrane, mainly composed of cellulose. It can be compared with the ceil wall of a plant ce

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd