Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

Vitality and vigour - qualitative characters, Vitality and Vigour - Qualita...

Vitality and Vigour - Qualitative Characters Vitality is related to the condition of a plant and its capacity to complete its life cycle, while vigor refers more specifically

Determine about the soil and its importance, Determine about the Soil and i...

Determine about the Soil and its importance Everyone knows about soil as a resource which fulfils the basic requirement of human kind by supporting variety of plants and other

Explain about central nervous system, Central Nervous System (CNS) Cove...

Central Nervous System (CNS) Covering of the brain and spinal cord is called Meanings (membranes which cover and protect brain and spinal cord are called meanings) There is flu

Determine the postovulatory phase of x menstrual cycle, Healthy human femal...

Healthy human female X is 25 years old and not pregnant.  During the postovulatory phase of X's menstrual cycle,   A.  there are high blood plasma levels of LH and FSH.

Digestive system, describe secretory function of salivary gland

describe secretory function of salivary gland

Syngamy and triple fusion, Syngamy and Triple Fusion After traversing...

Syngamy and Triple Fusion After traversing through the stylar region, the ultimate destination of the pollen tube is to reach the female gametophyte and release the male game

Use of syphilis in pregnancy, Syphilis in Pregnancy Syphilis in pregna...

Syphilis in Pregnancy Syphilis in pregnant women should be treated with penicillin in doses appropriate to the stage of the disease. When pregnant women with syphilis are alle

Terrestrial adaptation of some species of the phylum, Q. How can the featur...

Q. How can the features of the arthropod exoskeleton describe the terrestrial adaptation of some species of the phylum? In the arthropod exoskeleton there is a layer of wax whi

Explain what is fungi, Explain what is Fungi? The fungi are spore-beari...

Explain what is Fungi? The fungi are spore-bearing eukaryotic organisms without chlorophyll and having absorptive nutrition. These reproduce sexually as well asexually. Primari

How substrate concentration affect the enzymatic reaction, How does the sub...

How does the substrate concentration affect the speed of enzymatic reactions? Initially as substrate concentration increases, the speed of the reaction enhances; this happens b

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd