Preparation - culture medium, Biology

Assignment Help:

Preparation - Culture Medium

Now that you are familiar with the constituents the preparation of medium is quite simple. Several plant tissue culture media are now available commercially in the form of dry powders, containing all ingredients except growth regulators, sucrose and agar. They are very convenient to prepare media for routine maintenance of cultures. Generally concentrated stock solutions of major inorganic nutrients (200 times concentrated expressed as 20 x) micro inorganic nutrients (200 x concentrated) iron (200 x concentrated) and organic nutrients except sucrose are prepared and stored in a refrigerator at 4? c. Separate stock solutions are prepared and stored for each growth regulator by dissolving it in a minimal quantity of appropriate solvent and adjusting the final volume with distilled water.


Related Discussions:- Preparation - culture medium

DNA damage caused by ultraviolet light, DNA damage caused by ultraviolet li...

DNA damage caused by ultraviolet light: 1. Cyclobutane-type pyrimidine dimer is the major photoproduct formed 2. A second product, 6-4 photoproduct, is formed in about 10 % of UV i

Explain antimicrobial prophylaxis, Explain Antimicrobial Prophylaxis An...

Explain Antimicrobial Prophylaxis Antimicrobial prophylaxis can decrease the incidence of infection, particularly surgical site infection, after certain operations, but this be

Define reaction of glucose & fructose with fehlings reagent, Define Reactio...

Define Reaction of glucose and fructose with Fehlings reagent? Lactose is a milk sugar. It is a reducing disaccharide having a free sugar group and can reduce Fehling's reagent

Explain the parasympathetic neurons, Which of the following serves as an ac...

Which of the following serves as an actuating signal, or as part of an actuating signal, in a negative feedback system? A. Action potentials in the fibers of the diaphragm musc

Xerophytes, Xerophytes Xerophytes are sometimes loosely defineri as 'pl...

Xerophytes Xerophytes are sometimes loosely defineri as 'plants of dry habitats'. But others have defined xerophytes as 'plants which grow on the substratum that usually beco

What is diarrhoea, What is diarrhoea? Diarrhoea is characterized by th...

What is diarrhoea? Diarrhoea is characterized by the frequent evacuation of liquid stools, usually exceeding 300 ml, accompanied by an excessive loss of fluids and electrolyte

Demonstrate the cell membrane, While working in the lab, you isolate a cell...

While working in the lab, you isolate a cell from a mouse and a cell from a human and fuse them together to form a live, hybrid cell. You then use an antibody that recognizes all m

Explain activators, Activators Activity  of many  enzymes is  influence...

Activators Activity  of many  enzymes is  influenced by  certain ions called as activators. Large number of enzymes such as hexokinase that require ATF are also in need of diva

Ameoba, what is the scientific name of ameoba

what is the scientific name of ameoba

Distinct ecological importance of species, The distinct ecological importan...

The distinct ecological importance of species supports functional diversity and has a direct bearing on the species composition of the site, and therefore on overall biodiversity.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd