Precaution - separation of amino acid by paper chromatogrphy, Biology

Assignment Help:

Define Precaution for Separation of Amino Acids by Paper Chromatography?

1. Hold the paper with the extra strip kept at one side. Handling of whatman paper with hand should be avoided.

2. The spotting of amino acid should not be more than 1 mm in diameter since large spots lead to diffused and large amino acid patterns with no sharp outline.

3. The petridish should be saturated with solvent vapours.

4. Solvent front should be marked immediately after removing teh chromatogram, since it disappears rapidly.

5. The outline of each amino acid should be immediately marked with pencil, Do not mark with ball point or ink pen.


Related Discussions:- Precaution - separation of amino acid by paper chromatogrphy

Explain the cultural practices of single crop, Explain the Cultural Practic...

Explain the Cultural Practices of single crop Continuous cultivation of a single crop over years on the same site causes accumulation of a particular group of microbes, which d

Dna, What is a helix?

What is a helix?

How is the structure of a mitochondrion well adapted, How is the structure ...

How is the structure of a mitochondrion well adapted for the activities it carries out? The mitochondrial membranes segregate the enzymes and reactants of the Krebs cycle, faci

On site sanitation, Introduction: There are different technologies avai...

Introduction: There are different technologies available for dealing with waste water management and excreta.  Some are suitable for dealing off-site sanitation and other can b

Genetics, polymorphism and its causes

polymorphism and its causes

How do white cells differ from red cells, How do white cells differ from re...

How do white cells differ from red cells (a) In their structure, (b) Their function?   a) White cells have nuclei, red cells do not have nucle

Asphyxia, buffalo can be dies due ashyxia

buffalo can be dies due ashyxia

Risk factor modification, Risk Factor Modification :  Even after CABG,...

Risk Factor Modification :  Even after CABG, patients are at risk of progression of native coronary artery disease and development of lesions in the conduits. Modification of

Define briefly the enzymatic proteins, Define Briefly the Enzymatic Protein...

Define Briefly the Enzymatic Proteins? The most varied and very highly specialized proteins are those along with catalytic activity - the enzymes. Virtually all the chemical re

Ribosomes in eukaryotic, Q. How dissimilar are the location of ribosomes in...

Q. How dissimilar are the location of ribosomes in eukaryotic and in prokaryotic cells? In eukaryotic cells they can also be found free in cytoplasm and mainly adhered to the e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd