Precaution - separation of amino acid by paper chromatogrphy, Biology

Assignment Help:

Define Precaution for Separation of Amino Acids by Paper Chromatography?

1. Hold the paper with the extra strip kept at one side. Handling of whatman paper with hand should be avoided.

2. The spotting of amino acid should not be more than 1 mm in diameter since large spots lead to diffused and large amino acid patterns with no sharp outline.

3. The petridish should be saturated with solvent vapours.

4. Solvent front should be marked immediately after removing teh chromatogram, since it disappears rapidly.

5. The outline of each amino acid should be immediately marked with pencil, Do not mark with ball point or ink pen.


Related Discussions:- Precaution - separation of amino acid by paper chromatogrphy

Explain the basic concept of proteins, Explain the Basic Concept of Protein...

Explain the Basic Concept of Proteins? Proteins, as we already knows by now are products of amino acids. Each molecule of protein is composed of many molecules of amino acids j

Nuclear transfer method for gene transfer, Nuclear transfer method: Nucle...

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human

What is thermoregulation in cold, What is Thermoregulation in Cold? Hea...

What is Thermoregulation in Cold? Heat production parallels the increase in O 2 uptake, the magnitude of which depends on the muscle mass .engaged in slivering or work and the

What do you understand by compound eye, What do you understand by Compound ...

What do you understand by Compound eye? The characteristic eye of the Arthropoda. It comprise many ommatidia, the basic optical unit of compund eye, grouped together to form th

Chemicals of life, give two examples of chemical reactions which are cataly...

give two examples of chemical reactions which are catalysed by enzymes in the course of brewing

Express how that system accomplishes that necessity, Name a system that acc...

Name a system that accomplishes that necessity and explain how that system accomplishes that necessity.

Show non-modifiable risk factors for cardiovascular disease, Q. Show Non-Mo...

Q. Show Non-Modifiable Risk Factors for cardiovascular disease? Age: Earlier men less than 55 years were more prone but now heart disease has caught up with a younger age-gro

Zoonotic diseases-classification in terms of reservoir host, Classification...

Classification in terms of reservoir host 1.  Anthropozoonoses: Infections transmitted from lower vertebrate to man, e.g., rabies, plague, leptospirosis, etc. 2.   Zooanthro

Head examination of new born, Head - check for size, shape or fontan...

Head - check for size, shape or fontanelles, caput, or any other abnormality. Fontanel - anterior fontanel is diamond shaped having size of 2.5 - 4.5 cms. It close

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd