Precaution - separation of amino acid by paper chromatogrphy, Biology

Assignment Help:

Define Precaution for Separation of Amino Acids by Paper Chromatography?

1. Hold the paper with the extra strip kept at one side. Handling of whatman paper with hand should be avoided.

2. The spotting of amino acid should not be more than 1 mm in diameter since large spots lead to diffused and large amino acid patterns with no sharp outline.

3. The petridish should be saturated with solvent vapours.

4. Solvent front should be marked immediately after removing teh chromatogram, since it disappears rapidly.

5. The outline of each amino acid should be immediately marked with pencil, Do not mark with ball point or ink pen.


Related Discussions:- Precaution - separation of amino acid by paper chromatogrphy

Define role of leptin in controlling gene expression, Define role of Leptin...

Define role of Leptin in controlling gene expression? Leptin is a protein product (containing 167 amino acids) of the ob gene expressed exclusively in the adipose tissue. This

Zoology, difference between one girl and lungs two ribosomes and lysosomes ...

difference between one girl and lungs two ribosomes and lysosomes ?

What is the climax stage of an ecological succession, What is the climax st...

What is the climax stage of an ecological succession? The climax stage is the stage of the ecological succession in which the community of an ecosystem becomes stable and does

Explain about the sugaring - methods of food processing, Explain about the ...

Explain about the Sugaring - methods of food processing? Water is withdrawn from the microbial cells when they are placed in a strong sugar solution (about 68%) and thus result

Define procedure for measurement of ph, Define Procedure for Measurement of...

Define Procedure for Measurement of pH? To ensure reliability of the pH readings, you should first calibrate the pH meter. You should adjust the pH meter so that the readings a

What is global warming, What is global warming? Global warming is the e...

What is global warming? Global warming is the enhance in the temperature of the planet due to accumulation of some gases in the atmosphere, especially gases that retain the sol

Protozoa, What is meant by encystment

What is meant by encystment

Explain the cultural practices of single crop, Explain the Cultural Practic...

Explain the Cultural Practices of single crop Continuous cultivation of a single crop over years on the same site causes accumulation of a particular group of microbes, which d

State the limitations of intra oral periapical radiographs, Limitations of ...

Limitations of Intra Oral Periapical Radiographs  It is not optimal in pre operative planning of cases with severely resorbed ridges  Anatomical structure of importance can'

Phycomycosis (zygomycosis), Phycomycosis (zygomycosis) Zygomycosis (pr...

Phycomycosis (zygomycosis) Zygomycosis (previously known phycomycosis) involves fungi belonging to genera Mucor, Rhizopus, Absidia, Entomophthora, Mortierella and Basidiobolou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd