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The PCR (polymerase chain reaction) is a biochemical technology in molecular biology to intensify a one or a little copies of a piece of DNA across several orders of magnitude generating thousands to millions of copies of an exacting DNA sequence. It is developed in the year of 1983 by Kary Mullis, PCR is now a common and frequent indispensable method used in biological and medical research labs for several of applications. These contains DNA cloning for sequencing and DNA-based phylogeny, the diagnosis of hereditary diseases; functional analysis of genes; the recognition of genetic fingerprints which is used in forensic sciences and paternity testing and the detection and diagnosis of infectious diseases. In the year of 1993, the Mullis was awarded the Nobel Prize in Chemistry along with Michael Smith for his work on polymerase chain reaction.
Define Disadvantages of using yeast as a source of protein? 1. Less protein yield (45-60%) 2. Growth rate is low (1-3 h) 3. High nucleic acid content leading to the forma
Synovial fluid Collection: The technique of obtaining synovial fluid is called arthrocentesis. It is used to characterize type of arthritis and to differentiate inflammatory
Bone Remodeling Bone remodeling differs from the other means of bone structure alteration in that osteoblasts and Osteoclasts do not act independently but are coupled and bone
DRU G NOMENCLATURE - Generally drug has three types of names - Chemical, Proprietary & Non-proprietary. Chemical Name - Indicates chemical nature. Proprietary Name -
Germ line gene therapy - This approach either targets to correct the totipotent cells within human conceptus or stimulate the genetic modifications within reproductive cells that
Explain about the Net Protein Ratio (NPR)? This method was introduced by Bender and Doell (1957) and is a modification of the PER method. In this method, an allowance is made f
Q. What are the main bryophyte groups? The major bryophyte groups are the mosses, the hornworts and the liverworts.
Explain Pennate diatomsrole in diatoms? Pennate diatoms have bilateral symmetry, which presents side-by-side mirror images if divided down the middle or centerline, as seen
Explain the Microscope? Microscope is a powerful and crucial basic tool in the field of microbiology. Microbiology, as we have already studied in our theory Course, is the scie
Q What are the three major groups into which mammals are divided? The three groups into which mammals are divided are: monotremes (or prototherian that is platypus), placental
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