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The PCR (polymerase chain reaction) is a biochemical technology in molecular biology to intensify a one or a little copies of a piece of DNA across several orders of magnitude generating thousands to millions of copies of an exacting DNA sequence. It is developed in the year of 1983 by Kary Mullis, PCR is now a common and frequent indispensable method used in biological and medical research labs for several of applications. These contains DNA cloning for sequencing and DNA-based phylogeny, the diagnosis of hereditary diseases; functional analysis of genes; the recognition of genetic fingerprints which is used in forensic sciences and paternity testing and the detection and diagnosis of infectious diseases. In the year of 1993, the Mullis was awarded the Nobel Prize in Chemistry along with Michael Smith for his work on polymerase chain reaction.
Phases of Karyokinesis: Karyokinesis can further be divided into prophase, metaphase, anaphase and telophase. 1-Prophase: (pro= before) Chromatin: During Interphase (non
Why in the preparation of a karyotype analysis is the use of a substance like colchicine interesting? Colchicine is a substance that disallows the formation of microtubules and
Lakes - Lentic Ecosystems Most lakes occur in regions which have recently been subjected to geological changes; say within the past 20,000 years. However, a few lakes, such a
Explain holding method of pasteurization In the holding method of pasteurization (62 o C for 30 minutes) or the high-temperature short-time (HTST), 71 o C for 15 minutes method
a) What process is responsible for the movement of carbon dioxide into a leaf? b) In what conditions might the same process cause oxygen to enter a leaf? (a) Diffusi
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(a) What is the distinction among 'breathing' and 'respiration'? (b) What part do 'ventilation' and 'gaseous exchange' play in these processes? (a) Breathing i
Q. Explain the Rapport Building? Maintaining good relationship with the client is very important in counselling. This will help you to gain trust, confidence of the client and
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