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The PCR (polymerase chain reaction) is a biochemical technology in molecular biology to intensify a one or a little copies of a piece of DNA across several orders of magnitude generating thousands to millions of copies of an exacting DNA sequence. It is developed in the year of 1983 by Kary Mullis, PCR is now a common and frequent indispensable method used in biological and medical research labs for several of applications. These contains DNA cloning for sequencing and DNA-based phylogeny, the diagnosis of hereditary diseases; functional analysis of genes; the recognition of genetic fingerprints which is used in forensic sciences and paternity testing and the detection and diagnosis of infectious diseases. In the year of 1993, the Mullis was awarded the Nobel Prize in Chemistry along with Michael Smith for his work on polymerase chain reaction.
In designing an experiment to find out whether light is required for photosynthesis (a) what is the principle of the design (b) what control would you use?
Study of tonana larva Tornaria larva is typical in the life-history of hemichordates. Examine the permanent slide and note the following features: I. The tornaria larva usu
Question 1: Describe the need for Harmonization in clinical trials? Brief on the Revised ICH terms of reference and explain the structure of ICH? Need for Harmonization i
Define Mini Nutritional Assessment (MNA) Tool? It is a comprehensive and simple tool, which is able to categorize the subjects into three different categories like well nourish
Viral vectors There are a large number of viral vectors available which can transfer the desired sequence properly into the host. Examples of the viral vectors are as follows
Q. Can you explain Aneurysm of Aorta? CXR findings are an enlargement of the involved portion of the aorta. A focal dilatation may simulate a mass or adenopathy. A more general
Q. What are the hormones secreted by the adrenal cortex? What are their respective functions? The cortical portion of the adrenals secretes hormones of the corticoid (or cortic
Hypothermia As the blood passes through the oxygenator, hypothermia machine allows the blood to cool to the required temperature. Generally, the temperature is brought dow
Q. Define Hepatic Triglyceride Synthesis? Fatty acid flux to the liver from adipose tissue appears to be an important determinant of hepatic triglyceride synthesis and VLDL sec
Q. Etiological factor of peptic ulcer? Peptic ulcer results when the neural and hormonal abnormality disrupts the factors that normally maintain mucosal integrity and permit pr
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