Pollen biology, Biology

Assignment Help:

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.


Related Discussions:- Pollen biology

Blotting, Blotting  is the technique for detecting one RNA within the mixtu...

Blotting  is the technique for detecting one RNA within the mixture of RNAs (a Northern blot) or one type of the DNA within of a mixture of DNAs (a Southern blot). A blot can prove

What is the pineal gland, What is the pineal gland? The pineal gland, a...

What is the pineal gland? The pineal gland, also called as pineal body or epiphysis, is situated centrally in the head. It secretes the hormone melatonin, a hormone formed at n

Light stratification - lake ecosystem, Light Stratification - Lake Ecosyste...

Light Stratification - Lake Ecosystem The penetration of light in water bodies is limited depending on the transparency of water and its ability to absorb light. On the basis

Nutritional deficiency, Iron deficiency should be treated with supplemental...

Iron deficiency should be treated with supplemental iron. • Osteoporosis should be treated with calcium and vitamin D supplements. • Depending on individual factors, patien

Define advantages of using bacteria as a source of protein, Define Advantag...

Define Advantages of using Bacteria as a source of protein? Advantages of using Bacteria as a source of protein are as follow: 1. High yield of protein (60-80%) 2.

Draw decanol, Draw decanol, plamitic acid (C16:0 fatty acid) and the result...

Draw decanol, plamitic acid (C16:0 fatty acid) and the resulting wax ester generated by a dehydration reaction between both molecules.

Zoology, regenerstion in invertebrates

regenerstion in invertebrates

Human activity lead to the extinction of a species, In what ways could huma...

In what ways could human activity lead to the extinction of a species in an area? Human activity could lead to extinction of a species by (a) over-hunting, e.g. elephants, rhin

Define importance of nutrient and drug interaction, Define Importance of Nu...

Define Importance of Nutrient and Drug Interaction? Certain foods, beverages, alcohol, caffeine, and even cigarettes can interact with medicines. This may make them less effect

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd