Pollen biology, Biology

Assignment Help:

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.


Related Discussions:- Pollen biology

Microbes, what microbe is the one that is shaped like a ball with spikes co...

what microbe is the one that is shaped like a ball with spikes comming out

Explain about vitamins, Vitamins Vitamins, as you may already know, der...

Vitamins Vitamins, as you may already know, derive its name from the word 'Vital Amine'. These are the organic compounds required for normal metabolism, growth and developme

Define the thin and thick filaments of a sarcomere, When the overlap betwee...

When the overlap between the thin and thick filaments of a sarcomere in a skeletal muscle is decreasing, A. the total length of the I band is increasing in the sarcomere. B.

Cobalt deficiency, Cobalt deficiency The deficiency of cobalt is more ...

Cobalt deficiency The deficiency of cobalt is more commonly seen in ruminants fed on diets deficient in this essential micronutrient. Cobalt is stored only in limited amounts

Death, DE A T H - It is terminal end of the degeneration changes of ...

DE A T H - It is terminal end of the degeneration changes of old age. Death occurs when vescular system & respiratory system stop working. Failure of nervous system ma

Explain homologous and heterologous immunoglobulins, What is the difference...

What is the difference between homologous and heterologous immunoglobulins? Homologous immunoglobulin is the human (from the similar species) immunoglobulin. In case of inocula

What is the window phase of an infection, Q. What is the window phase of an...

Q. What is the window phase of an infection? How is this concept important for the test of HIV infection in blood banks? The primary immune reply of the body facing any infecti

Explain how is cancer related to ras, Can someone explain how is cancer rel...

Can someone explain how is cancer related to ras and p53 gene? I know that the ras protein regulate cell growth and its mutation will cause cancer. How is the mutation caused?

Define nitrogen requirements in pregnancy and lactation, Define Nitrogen re...

Define Nitrogen requirements in Pregnancy and Lactation? The nitrogen accretion in the pregnant woman (assuming that the foetus weighs 3.3 kg at term), estimated by the Committ

Define antioxidant activity of manganese, Define Antioxidant Activity of ma...

Define Antioxidant Activity of manganese? As Mn is a component of mitochondrial Superoxide Dismutase (SOD), it can protect against oxidative damage. In-vitro experiments have i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd