Pollen biology, Biology

Assignment Help:

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.


Related Discussions:- Pollen biology

Define evolution of food processing, Define Evolution of food processing? ...

Define Evolution of food processing? 8000 - 7000 BC. Mankind first began farming, growing crops and raising animals for food instead of hunting and gathering for food.

Find equilibrium potential of a particular ion, Which of the following is N...

Which of the following is NOT required to determine equilibrium potential of a particular ion? Select one: size of the ion valence of the ion concentration gradient for

Explain polio, Explain Polio Adults who have not previously been immuni...

Explain Polio Adults who have not previously been immunized against polio should receive a primary series of inactivated polio vaccine (IPV) if traveling to areas where polio i

Flowering - development of plant, Flowering - Development of plant One...

Flowering - Development of plant One of the major changes that occur during the life cycle of a plant is the transition from vegetative stage to the flowering stage. In this t

Dideoxy sequencing, Dideoxy Sequencing is the enzymatic determination and ...

Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminati

Skeleton, what are the biological significance of skeleton

what are the biological significance of skeleton

Shoot multiplication -clonal propagation, Shoot Multiplication -Clonal Prop...

Shoot Multiplication -Clonal Propagation This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and

Explaintransketolase, ExplainTransketolase Transketolase with the help...

ExplainTransketolase Transketolase with the help of TPP  and Mg ++   is  required again. This  time  it  transfers carbon 1+2 from xylulose-5-phosphate  to erythrose-4-phospha

Pvcs during recovery, As the heart rapidly slows during recovery from exerc...

As the heart rapidly slows during recovery from exercise, PVCs commonly occur and usually have no clinical significance. This may be a time when metabolic adjustments in the heart

Animal nutrition, what steps can you take in order to assess why your cattl...

what steps can you take in order to assess why your cattle are not drinking enough water

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd