Pollen biology, Biology

Assignment Help:

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.


Related Discussions:- Pollen biology

Important causes of degradation of ecosystem, Important Causes of Degradati...

Important Causes of Degradation of Ecosystem The important causes that have lead to the degradation of the ecosystem. Deforestation Overgrazing Agriculture

Zygotic or suspensor polyembryony, Zygotic or Suspensor Polyembryony ...

Zygotic or Suspensor Polyembryony Cleavage of the apical cells of the globular or filamentous proembryo produced by the zygote may result in two or more embryos in a seed. Th

basic phases of pcr, What are three basic phases of PCR?  Which phase of t...

What are three basic phases of PCR?  Which phase of these three phases is used for analysis for the end-point PCR? Why? Which phase is used for analysis for the quantitative real-t

Explain protoderm in primary growth in shoot, Explain Protoderm in primary ...

Explain Protoderm in primary growth in shoot? The protoderm is one of the so-called "primary tissues" because it is formed first during germination and subsequent bud growth an

What are the part of the musculoskeletal system, Which are the organs that ...

Which are the organs that are part of the musculoskeletal system? The main organs and tissues that are part of the musculoskeletal system in humans are the cartilages, the bone

State the copious sterile saline irrigation, Using copious sterile saline i...

Using copious sterile saline irrigation This can be delivered from a sterile infusion bag in a pressure cuff or a peristaltic pump. The drills can be adequately cooled by spray

What factor helps us in identifying the type of bone graft, What factor hel...

What factor helps us in identifying the type of bone graft to be used? The following factors help us in identyfying the bone graft to be used: i) Whether graft is osteoinduc

Example of trial balance columns, Q. Example of Trial Balance columns? ...

Q. Example of Trial Balance columns? Start by extending all of Micro Train's revenue and expense account balances in the Adjusted Trial Balance columns to the Income Statement

Blood flow, if i start in the superior mesenteric vain where do i have to g...

if i start in the superior mesenteric vain where do i have to go through to get to the left anterior circumflex humeral artery. by the way of the hepatic portal vein and the heart

Differential reinforcement of other behaviour, Differential reinforcement o...

Differential reinforcement of other behaviour (DRO) This is used to decrease frequent behaviour by reinforcing any behaviour other than the undesired one. An instance would be r

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd