Pollen biology, Biology

Assignment Help:

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.


Related Discussions:- Pollen biology

Explain the biography of pedanius dioscorides, Explain the Biography of Ped...

Explain the Biography of Pedanius Dioscorides? Pedanius Dioscorides, a Cicilian Greek, who lived in the first century A.D. was the most important botanist after Theophrastus. H

Schematic representation of curser position of pulse wave, A lot of hemodyn...

A lot of hemodynamic information including maximum and mean velocity, maximum and mean pressure gradients and pressure half time can be obtained from Doppler trace that helps in

What is the mean of crp, CRP is a marker of systemic inflammation. As the r...

CRP is a marker of systemic inflammation. As the role of inflammation in the initiation and progression of atherosclerosis becomes better understood, CRP has gained prominence as a

Disposal-lab animals, Disposal : Opaque bags are the recommended for dispo...

Disposal : Opaque bags are the recommended for disposing off carcasses. You should also put freshly dissected animals and tissues into opaque plastic bags, seal them and dispose t

What is oligosaccharides , Oligosaccharides are short chains of m...

Oligosaccharides are short chains of monosaccharides linked together by glycosidic bonds. In case of oligosaccharides  linked to proteins (glycoproteins)  or lipids (glycolipids) o

Knocking and octane number, Mixture of gasoline and air is used as fuel in ...

Mixture of gasoline and air is used as fuel in internal combustion engine. It is highly compressed and then ignited. 1.      The process involves four strokes : 2.      The f

Explain karyotype - animal taxonomy, Explain Karyotype - Animal Taxonomy? ...

Explain Karyotype - Animal Taxonomy? In animals systematic, it is the karyotype, that is the chromosome number and structure which are used quite extensively either separately

Explain procedure for test of presence of coliform bacteria, Explain Proced...

Explain Procedure for the test of Presence of Coliform Bacteria? This test procedure is in continuation to the presumptive test procedure followed on last experiment. Now carry

Infectious and chronic diseases, Infectious and Chronic Diseases The m...

Infectious and Chronic Diseases The most prevalent diseases of poverty, many of which are also infectious by nature, are malaria, tuberculosis, respiratory infections, water b

Genetic material, Assuming that variability of populations were non-genetic...

Assuming that variability of populations were non-genetic, that is, not controlled by genetic material, once again chance events alone would determine which of the organisms would

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd