Pollen biology, Biology

Assignment Help:

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.


Related Discussions:- Pollen biology

Twins, how twins are produced and why

how twins are produced and why

Advantage and disadvantages of fensom and spanner, Advantage and disadvanta...

Advantage and disadvantages of Fensom and Spanner Electroosmotic Flow Hypothesis The model has several advantages over Munch's model. The presence of P- proteins, and the occl

Explain the serial dilution process - plate counts, Explain the Serial Dilu...

Explain the Serial Dilution Process - Plate Counts? Dilution factor is the reciprocal of the dilution used. As number of microorganisms in original sample is not known, the ori

Green fluorescent protein - mitochondrial inner membrane, You are studying ...

You are studying how proteins are targeted to the mitochondria. MIP1 is in the mitochondrial inner membrane, whereas MIP2 is targeted to the matrix. You are most interested in how

Determine the functional food in europe, Determine the Functional Food in E...

Determine the Functional Food in Europe? Functional foods are: Conventional/everyday foods consumed as a part of the normal diet; Composed of naturally-occurring co

What is revisions, Q. What is Revisions? Revision accounts for only a s...

Q. What is Revisions? Revision accounts for only a section of a genus or for the elements as restricted to a continent or smaller geographical area. Some revisions are based so

What is the structural representation of a carboxyl group, What is the stru...

What is the structural representation of a carboxyl group? Carboxyl groups have a carbon attached to single hydroxyl group by a simple bond and to one oxygen by a double bond.

Digestive system of class asteroidea, Digestive System of Class Asteroidea ...

Digestive System of Class Asteroidea Most asteroids are scavengers or carnivores and feed on snails, bivalves, polychaets, other echinoderms, fish, sponges, sea anemones and p

How much milk require by patient in a day, How many protein require patien...

How many protein require patient in a day Patients who require high protein diets are encouraged to drink 600-800 mL of milk a day. Now, you must be wondering how one can consu

Importance of research in nursing, Importance of Research in Nursing: ...

Importance of Research in Nursing: It is  the responsibility of the nursing profession  to discover, verify, structure and restructure the professional knowledge through  syst

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd