Pollen biology, Biology

Assignment Help:

Pollen Biology

Fluorescence microscopy coupled with biochemical analysis has helped resolve the differential activity of the enzyme, β-l, 3- glucanase that catalyses the dissolution of callose. The enzyme activity is relatively low during the first meiotic division but reaches its peak at the time of spore release. This sequence of events controlling the enzyme activity is important, as an early dissolution of callose may lead to sterility. Use of autoradiographic and fluorochromatic techniques demonstrated the reduced permeability of the callose wall and the resultant chemical isolation of microspores.

A combination of phase-contrast and fluorescence microscopy also revealed that the callose wall during exins formation serves as a mold or template. Factors that trigger the onset and regulation of meidsis in anther and ovule can be worked out by employing labeled reagents. Biochemical studies through isoenzyme markers have already demonstrated specific stages of meiotic process. There is an increase in the activity of acid phosphatase at zygotene, followed by subsequent decline. At pachytene, marked activation of endonucleases is recorded. Isoperoxidases serve as markers in normal pollen development in Mercurialis annua.


Related Discussions:- Pollen biology

Define hydration properties of proteins, Define Hydration Properties of Pro...

Define Hydration Properties of Proteins? General conformation of individual proteins in solution is largely dependent on the interaction with water. The progressive hydration o

Causes of hypoglycaemia, Since hypoglycaemia can occur in any diabetic pati...

Since hypoglycaemia can occur in any diabetic patient at any time, it is important for you to learn the causes of hypoglycaemia. Such knowledge will help you to educate the patient

Determine requirements of vitamins at high altitude, Determine requirements...

Determine requirements of Vitamins at high altitude? It is observed that requirement of vitamins is not different as compared to plains. In humans, exposure to high altitude ha

Explain voriconazole, Explain Voriconazole It has a spectrum of activit...

Explain Voriconazole It has a spectrum of activity similar  to itraconazole but appears to be more active against Aspergillus spp. And some species of  Candida, containing  C.

How folate is important for pregnant women, How Folate is Important For Pre...

How Folate is Important For Pregnant Women? Folate is also important for pregnant women. Low blood levels of folate during pregnancy can cause neural tube defects-anencephaly (

Law of diminishing return, Law of Diminishing Return A German scientis...

Law of Diminishing Return A German scientist Mitscherlich calculated the effect of different quantities of various growth factors on the yield of plants by means of a mathemat

Limb regeneration in amphibians, Limb Regeneration in Amphibians In v...

Limb Regeneration in Amphibians In vertebrates, the amphibians particularly the urodeles comprise spectacular power of regeneration. This power of regeneration has made them

Define protection against poisoning - function of vitamin e, Define Protect...

Define Protection against poisoning - Function of Vitamin E? 1) Protection against poisoning: It protects liver from injury due to carbon tetra chloride poisoning. 2) Pr

Explain advantages of using bacteria as a source of protein, Bacteria:- ...

Bacteria:- Advantages High yield of protein (60-80%) Can be grown on paraffin, cellulose waste and molasses. Growth rate is fast. Disadvantages  If the bacteri

Clinical features - rheumatic fever, The American Heart Association (AHA) h...

The American Heart Association (AHA) has recommended the revised Jones Criteria as a guide for ARF diagnosis. The same have been approved by WHO study group for the diagnosis of in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd