Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Rassenkreis and speciation, According to the current concept of speciation,...

According to the current concept of speciation, a widely distributed species should break up into partially isolated rule species. The different subspecies become differentiated fu

Describe promoter motifs commonly associated with eukaryotic, Gene expressi...

Gene expression must begin with the process of transcription a)  Describe the promoter motifs commonly associated with eukaryotic protein-coding genes, and explain their influen

Explain the appearance of the pupil, Explain the Appearance of the Pupil ...

Explain the Appearance of the Pupil The pupil should appear round and regular. The normal diameter  is  2-4 mm. On stimulation by  light,  the pupil  constricts, so as  to redu

Crisis due to deforestation, Crisis due to Deforestation Due to conti...

Crisis due to Deforestation Due to continuing deforestation we are faced with a major ecological and socio-economic crisis. To reverse this trend of deforestation a National

Explain gum tragacanth, Gum Tragacanth  Gum tragacanth, the exudation o...

Gum Tragacanth  Gum tragacanth, the exudation of Astragalus species, is defined as the "dried gummy exudate" obtained from Astragalus gummifer Labillardiere. Gum tragacanth is

Parasitic diseases - fascioliasis, P a r a s i t i c Diseases ...

P a r a s i t i c Diseases F a s cioliasis It is a liverfluke infestation characterized by hepatic insufficiency, bile duct obstruction, and poor production per

What are the corals, Q What are the corals? Corals are characterized by...

Q What are the corals? Corals are characterized by their polypoid shape, slow growth and sessility and secretion of a solid skeleton made of calcium carbonate. Numerous coral i

What are the main biological functions of water, Q. What are the main biolo...

Q. What are the main biological functions of water? Ans. Water is the basic solvent for chemical reactions of living beings; it is the main means of substance transportati

What is the results of congenital pulmonary stenosis, What is the Results o...

What is the Results of Congenital Pulmonary Stenosis? Early mortality for open pulmonary valvotomy in neonates varies between 6 and 10 per cent. For infants and children, surgi

Explain fluconazole, Fluconazole Fluconazole is active against most  Ca...

Fluconazole Fluconazole is active against most  Candida species other than C. krusei, which is intrinsically resistant, and many strains of C. glabrata, which are increasingly

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd