Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Heteroduplex dna, Heteroduplex Dna  is generated by the base pairing betwee...

Heteroduplex Dna  is generated by the base pairing between complementary single strands derived from the different parental duplex molecules; heteroduplex DNA molecules occuring du

Name the parts of plant tissue, Which is the plant tissue responsible for t...

Which is the plant tissue responsible for the filling of the space between other tissues? The plant-filling tissue is generically known as parenchyma. The plant parenchyma can

What is phenotypic ratio, The F2 phenotypic ratio from a dihybrid cross wit...

The F2 phenotypic ratio from a dihybrid cross with epistasis was 9:3:4. If instead of a dihybrid cross, a double heterozygote was test crossed (crossed to a homozygous recessive in

What is biological magnification, a) What is biological magnification? ...

a) What is biological magnification? b) Describe how DDT as a water pollutant undergoes biological magnification?

Defects of heart, DEFECT S OF HEART 1 .      Blue Baby syndrome (Cya...

DEFECT S OF HEART 1 .      Blue Baby syndrome (Cyanosis) - Due to persisting foramen ovalis in atrial septum even after birth, the impure blood from right auricles comes to

Amino acid sequence, Amino acid sequence  is also known as the primary stru...

Amino acid sequence  is also known as the primary structure of a protein/polypeptide; the series of amino acids in a protein/polypeptide controlled by the series of DNA bases.

Protein prediction., I am trying to get help on the creation of a dataset f...

I am trying to get help on the creation of a dataset for protein prediction in matlab

Autotomy and regeneration, Autotomy and Regeneration Shedding of body...

Autotomy and Regeneration Shedding of body parts in self-defense to avert the attention of the predator-enemy or in any other emergency is a type of autotomy (auto: self, tom

Protazoa, In what order did the four phyla of protazoa evolve and why in th...

In what order did the four phyla of protazoa evolve and why in this order?

Tolerance range, what is the tolerance range of man and tillapia

what is the tolerance range of man and tillapia

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd