Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Aims and applications of epidemiology, Problem 1: Show the aims and app...

Problem 1: Show the aims and applications of Epidemiology. Definition of Epidemiology. List aims of Epidemiology List applicationsof Epidemiology Problem 2

Define precaution-estimation of nitrogen and protein content, Define Precau...

Define Precautions - Estimation of Nitrogen and Protein Content? 1. The apparatus should be airtight to prevent leakage of NH 3 . 2. The tip of the delivery tube should be i

Angiotensin - vertebrates, Normal 0 false false false E...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Deficiency diseases-iodine deficiency, Iodine  deficiency Iodine is an...

Iodine  deficiency Iodine is an important component of thyroid hormones, which play active role in several physiological process such as control of basal metabolic rate, oxidat

Determine st-segment elevation, Q. Determine ST-Segment Elevation? Ans...

Q. Determine ST-Segment Elevation? Ans. Exercise induced ST-segment elevation is common in subjects with post MI stress tests in leads where Q-waves are present. It has be

Problem of survival among cheetahs, Once quite widespread throughout Asia a...

Once quite widespread throughout Asia and Africa, there has been a large reduction of the cheetah's habitat and numbers though it is still seen in widely separated areas of Africa.

What are the antigens and antibodies of abo blood system, What are the anti...

What are the antigens and antibodies of each blood type of the ABO blood system? Type A: antigen A, antibody anti-B. Type B: antigen B, antibody anti-A. Type AB: antigens A and

HEALTHY DIET, EXPLAIN THE CONSTITUENT OF FOOD GROUPS AND THEIR SOURCES

EXPLAIN THE CONSTITUENT OF FOOD GROUPS AND THEIR SOURCES

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd