Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

How is energy transferred along a food chain?, How is energy transferred al...

How is energy transferred along a food chain? The energy flux beside a food chain is always unidirectional, from the producers to the decomposers.

What is eucalyptus dive, To verify the effect of intra-specific competition...

To verify the effect of intra-specific competition on the growth of saplings of Eucalyptus dives, an experiment was designed in which two sets of pots were used. In the first set o

What we need undergloves, Q. What we need Undergloves? These are a cott...

Q. What we need Undergloves? These are a cotton glove worn under the treatment glove for those operators experiencing discomfort to the skin on the hands. These must be replace

Lampbrush chromosomes, LAMPBRUS H CHROMOSOMES Discovered by Rucker...

LAMPBRUS H CHROMOSOMES Discovered by Ruckert . Lampbrush Chromosomes were seen in Oocytes of several different animals like shark, amphibians, reptiles and birds. (Most

Explain the evolution of the vascular plant body, Explain the Evolution of ...

Explain the Evolution of the Vascular Plant Body? Vascular Plant anatomy reflects adaptation to life on land. In an aquatic environment, the photosynthetic surfaces are support

Define precipitation or solubility of proteins, Define Precipitation or Sol...

Define Precipitation or Solubility of Proteins? Most of the functional properties are dependent on the degree to which the proteins are soluble. The solubility behaviour provid

Phylum chordata, main subdivision of phylum chordata

main subdivision of phylum chordata

Determine the assess mobility of prosthesis, Assess Mobility of Prosthesis ...

Assess Mobility of Prosthesis All implant-supported prostheses must be stable.  To detect mobility, place an explorer or scaler under the embrasures and apply gentle pressure i

Define the single cell proteins (scp), Define the Single Cell Proteins (SCP...

Define the Single Cell Proteins (SCP)? You may have heard of SCP. What is a single cell protein? Let's find out. The term SCP was coined by Prof. Caroll Wilson (MIT) in 1966. I

How dna-rna hybridization occurs, Q. How DNA-RNA hybridization occurs B...

Q. How DNA-RNA hybridization occurs Both DNA and RNA are able to form hybrids in solution with other RNA or DNA molecules which have complementary base pairing. Double-stranded

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd