Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Biphasic graph, how can i explain the biphasic graph i get fom combination ...

how can i explain the biphasic graph i get fom combination of two drugs?

A decrease in parasympathetic discharge to the heart, A decrease in parasym...

A decrease in parasympathetic discharge to the heart leads to A. a decrease in the conductance of F-channels in SA node cells. B. an increase in the conductance of potassium

Spermatogonia - spermatozoa, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 Spermatogonia - Spe

Cnidarians: obelia, why is obelia considered to be of special interest in Z...

why is obelia considered to be of special interest in Zoology as an animal showing an intermediate grade of organisation

Symptoms of chronic gastritis, Q. Symptoms of chronic gastritis? These ...

Q. Symptoms of chronic gastritis? These include anorexia, chronic fatigue, and feeling of fullness, belching, vague epigastric pain, nausea and vomiting and passage of black ta

Describe the importance of neuropsychological tests, Describe the importanc...

Describe the importance of Neuropsychological tests Neuropsychological tests are generally thought of as assessment instrument for a variety of cognitive, perceptual, and motor

Circulatory systems in animals, Circulatory Systems in Animals In anim...

Circulatory Systems in Animals In animals that have efficient circulatory systems and readily permeable vascular skins, gas exchange occurs through the integument. Thus we fin

Haversian canals and the volkmann canals of the bones, Q. What are the Have...

Q. What are the Haversian canals and the Volkmann's canals of the bones? Is the osseous tissue vascularized? The Haversian canals are longitudinal canals present in the osseous

Functions of synergids, Functions of Synergids Ultra structurally the ...

Functions of Synergids Ultra structurally the structure and concentration of cell organelles reveals that synergids are metabolically active. The following Wee functions are a

Which of the groups is not ionizable, Which of the following groups is NOT ...

Which of the following groups is NOT ionizable? Select one: a. Guanidinium b. Imidazole c. Phosphoryl d. Amine e. Aldehyde

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd