Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

How to increase the levels of physical activity, How to increase the levels...

How to increase the levels of physical activity? The benefits of physical fitness should be spread among the public through mass media. 1. Physical activity should be encour

Describe the general guidelines for use of bone grafting, General guideline...

General guidelines for use of BONE GRAFTING 1. The surgical site should be prepared, that is it should be free of granulation tissue. 2. For autogenous grafts- the donor sit

Tools of lower paieolithic age, Tools of lower paieolithic age Pleistoc...

Tools of lower paieolithic age Pleistocene times there were huge herds of very large mammals. Bones of such large mammals were found associated with the human fossil finds and

Describe new cardio-vascular risk factors, Describe New Cardio-vascular Ris...

Describe New Cardio-vascular Risk Factors ? The major risk factors contributing to the development of atherosclerotic plaques in blood vessels have been known for many years. H

Spina bifida cystica, Spina Bifida Cystica There is a protrusion of me...

Spina Bifida Cystica There is a protrusion of meninges and spinal cord a) Meningocele- it is relatively uncommon lesion (4-5 per cent). In this there is protrusion of me

Process of evolutionary change, In that Unit you also learnt about Darwin, ...

In that Unit you also learnt about Darwin, who based on his extensive observations and interpretations showed that evolution is a simple fact of nature. It was Theodosius Dobzhansk

Digestive enzymes, Digestive Enzymes Now let us consider the general p...

Digestive Enzymes Now let us consider the general principles of digestion that are applicable t6all animals. We will start with the digestive enzymes that breakdown the large

What is retrograde peri-implantitis, Retrograde Peri-Implantitis It has...

Retrograde Peri-Implantitis It has been described by Misch as implant failure probably due to bone microfractures caused by premature implant loading or overloading, other form

Define phenolic acids and derivatives, Define Phenolic Acids and Derivative...

Define Phenolic Acids and Derivatives? Two families of phenolic acids are widely distributed in plants: a range of benzoic acid derivatives and those derived from cinnamic acid

Synthesis and storage of hormone, Synthesis and Storage of Hormone The...

Synthesis and Storage of Hormone The synthesis and storage of hormone depends on the nature of the hormone. Steroid hormones are secreted in diffuse molecular form and usually

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd