Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Providing critical resources - conservation of wildlife, Providing Critical...

Providing Critical Resources - Conservation of Wildlife Another way to improve the habitat of a threatened species is to determine which resource is limiting the population si

Describe the factors that affect cardiac, Describe the factors that affect ...

Describe the factors that affect cardiac output in a female athlete who is speed skating toward the finish line in an Olympic race.

Objectives of dietary management of myocardial infarction, Q. Objectives of...

Q. Objectives of dietary management of myocardial infarction? The objectives of dietary management of myocardial infarction patients are as follows: - To provide rest to the

The advantages of human milk over cow milk, a) What are the advantages of h...

a) What are the advantages of human milk over cows' milk for feeding babies? b) Apart from the composition of the milk, what are the other advantages of breast- feeding?

What is pulmonary veins in normal pulmonary vasculature, Q. What is Pulmona...

Q. What is Pulmonary Veins in Normal Pulmonary Vasculature? The right and left upper lobe or superior pulmonary veins descend lateral to the arteries, cross in front of the hil

Enumerate the history of neuropsychological, Enumerate the history of neuro...

Enumerate the history of neuropsychological The typical neuropsychological exam begins with a careful history taking. Areas of interest include: Medical history of patie

Excretory system - kidney, Kidney - 2 in number. Dark red. Sl...

Kidney - 2 in number. Dark red. Slightly flattened. Bean shaped. 10 cm. long, 5 cm wide and 4 cm thick (In rabbit 20-25 mm). Weighing 150 gm in male 135 gm in fema

Chromosomes with spindle fiber, Chromosomes with spindle fiber: The kineto...

Chromosomes with spindle fiber: The kinetochore fibers of spindle attach to the kinetochore region (specialized area in centromere) of chromosome, and align them at the equator of

State the posterior superior alveolar nerve, Posterior superior alveolar ne...

Posterior superior alveolar nerve and vessels It is found in the  posterior aspect of the maxilla running between the bone and the lining of the maxillary sinus may be injured

Procedure for use microbiological laboratory equipment, Define Procedure fo...

Define Procedure for use Microbiological Laboratory Equipments? Now carry out this activity following the steps given herewith: 1. Observe each instrument (as mentioned abov

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd