Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Explain urine urea nitrogen - nutrition during stress, Explain Urine Urea N...

Explain Urine Urea Nitrogen - Nutrition during Stress? 5 gm/24 hrs. = No stress 5 to 10 gm/24 hr = mild hyper-metabolism or level 1 stress 10 to 15 gml24 hrs = moderat

Portion of double-stranded dna, Suppose that a portion of double-stranded D...

Suppose that a portion of double-stranded DNA in the middle of a large gene is being transcribed by an RNA polymerase. As the polymerase moves through the sequence of six bases sho

Determine the food sources of iodine, Determine the Food Sources of Iodine?...

Determine the Food Sources of Iodine? Please note that unlike other minerals studied so far, like selenium, the iodine concentration in foods is highly variable and also depend

Brackish water-marine water-types of water found on earth, Brackish Water ...

Brackish Water The content of dissolved salts in brackish water is higher than the fresh water and ranges between 0.5 to 35%. These waters of intermediate salinity range are d

Common disorders of coronary heart diseases, Q. Common Disorders of Coronar...

Q. Common Disorders of Coronary Heart Diseases? We shall deal with different types of heart diseases, their- etiology, symptoms, complications, as well as, nutrition and dietar

Explain procedure for detection of metanil yellow, Explain Procedure for De...

Explain Procedure for Detection of Metanil Yellow? Carry out the exercise following the steps enumerated herewith. 1. Suspend the food sample in water and shake vigorously.

Leaves give off water vapour, Leaves give off water vapour Use two same...

Leaves give off water vapour Use two same pots of soil, one with a small plant and the other without. Cover the soil in every pot with cardboard as shown in the diagram after w

Indications for surgery- pulmonary stenosis, Indications for Surgery :  Co...

Indications for Surgery :  Congenital pulmonic stenosis is the most common lesion requiring relief. Very rarely it could be due to rheumatic involvement along with disease of othe

Ventricular septal defect, A newborn baby with a patent foramen ovale or a ...

A newborn baby with a patent foramen ovale or a ventricular septal defect might be cyanotic (blue). Will a two-year-old with these defects also be cyanotic? Explain your answer.

Explain in brief - chitinous, Explain in brief Chitinous? A structure w...

Explain in brief Chitinous? A structure which is composed of chitin, a complex carbohydrate composed of linearly arranged Nacetyl-glucosamine units. Chitin is a characteristic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd