Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Role of dietitian in health care, Role of Dietitian in Health Care The ...

Role of Dietitian in Health Care The role of the dietitian has come a long way since the early 1900s. Their role is still unknown to a  lot of people. Some think that dietitian

Radiobiology, Radiobiology : This is the study of effects of radiations on ...

Radiobiology : This is the study of effects of radiations on living organisms. Radiobiology is a branch of science which concerned with the action of ions. Radiobiology is the radi

How many meters long is the molecule, E. coli DNA has a MW of about 2.7 x 1...

E. coli DNA has a MW of about 2.7 x 10^9 Daltons. How many meters long is this molecule and what does this tell you about the state of the intracellular DNA? ( The lenght of a sing

What is interpretation of stress echocardiography, Q. What is Interpretatio...

Q. What is Interpretation of Stress Echocardiography? The standard approach to interpretation is qualitative. The attendant need for training and problems posed for reproducibi

VASCULAR PLANTS., WHAT TRAITS ALLOWED VASCULAR PLANTS TO GROW TALL

WHAT TRAITS ALLOWED VASCULAR PLANTS TO GROW TALL

Fat burn, how to burn more and more adipose tissues

how to burn more and more adipose tissues

Define traditional cycle of root canal treatment, Define Traditional Cycle ...

Define Traditional Cycle of Root Canal Treatment The traditional cycle of Root Canal Treatment a) Dig in the tooth to make a big hole b) Then, look through this hole to f

Lipids, How are lipids classified?

How are lipids classified?

Determine some assessment measures of zinc, Determine some assessment measu...

Determine some assessment measures of Zinc? Measurement of zinc in plasma: This is the most common method. Fasting concentrations of less than 70 μg/litre suggests deficie

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd