Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

BIOLOGY, 100 WORDS NOT COMPLETED

100 WORDS NOT COMPLETED

What are diseases of the connective tissue, Q. What are diseases of the con...

Q. What are diseases of the connective tissue? What are some of them? Diseases of the connective tissue are acquired or hereditary diseases numerous of autoimmune cause charact

What is menu planning, What is Menu Planning? Any individual who carrie...

What is Menu Planning? Any individual who carries the responsibility of providing meals has to take decisions regarding what to serve, how much to serve, how much to spend, whe

Define the parathyroid gland cells, Which of the following serves as a sens...

Which of the following serves as a sensor, or as part of a sensor, that functions in a negative feedback system? A. CaSRs (Calcium-Sensing Receptors) located in the plasma memb

Hearing, HEARIN G  - Sound waves struck ear drum, it starts vibratio...

HEARIN G  - Sound waves struck ear drum, it starts vibrations, these vibrations are carried to MIS → f. ovalis → S. vestibule →  Hellicotrema → S. Tympani → f. rotundus → mi

Determine the food-searching activity, The Svalbard ptarmigan (Lagopus mutu...

The Svalbard ptarmigan (Lagopus mutus hyperboreus) is a small herbivorous bird that lives permanently on the island chain of Svalbard. Svalbard is located in the Arctic Circle, app

What is the major cell organelle involved in cell digestion, Q. What is the...

Q. What is the major cell organelle involved in cell digestion and what are the properties of that organelle that enable it to do the task? The organelles accountable for intra

Why do roots of many swamp plants have a special morphology, Why do roots o...

Why do roots of many swamp plants have a special morphology? Swamp and marsh plants usually present supporting roots that ramify from portions of the stem above the ground help

Nutrient and storage protein -biological function of protein, Define Nutrie...

Define Nutrient and Storage protein - biological function of protein? These proteins store amino acids. Ovalbumin is the protein of egg white, employed as an amino acid source

Explain functional property of emulsification, Explain Functional property ...

Explain Functional property of Emulsification Mode of action  Proteins stabilize fat emulsions Food system Sausages, soups, cakes, salad dressings, infant foods

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd