Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Explain Genetic equilibrium for genes with two alleles, What is the mathema...

What is the mathematical expression of the genetic equilibrium for genes with two alleles? Is this statistical distribution the same as the statistical distribution of the respecti

Possible recombinant genotypes, A woman is heterozygous for three X-linked ...

A woman is heterozygous for three X-linked loci: AaBbDd. She has seven sons with the following genotypes: two are X ABd Y three are X abD Y one is X ABD Y one is X Abd

Protein folding, Protein Folding With all the known information of prot...

Protein Folding With all the known information of protein structure from X-ray and NMR studies and one might expect in which we would know how proteins fold up within structure

Biosphere, The biosphere is composed of all living organism. This sphere in...

The biosphere is composed of all living organism. This sphere includes all the microorganism, plants and animals of earth. In fact biosphere involves interaction of living beings w

Dna vs protein, Describe the difference between dna and protein. (structura...

Describe the difference between dna and protein. (structural unit, linkage bond, primary and spatial structure)

Determine the human genome project, List three important discoveries that r...

List three important discoveries that resulted from the Human Genome Project. Answers should contain three of the following: Only about 2 percent of the human genome encodes p

Discuss about nerve to mylohyoid, Nerve to Mylohyoid Motor branch of i...

Nerve to Mylohyoid Motor branch of inferior dental nerve which descends in a groove on the medial surface of the mandibular ramus. Surgical intervention in this area may lead

Body cavity and coelom - metazoa, Body Cavity and Coelom - Metazoa Vac...

Body Cavity and Coelom - Metazoa Vacuoles, spaces, lacunae and cavities have been of importance in all organisms, may it a be plant or animal. All animals have cavities. The c

Benefits of haccp, The benefits of HACCP  can be  summarized  as under. ...

The benefits of HACCP  can be  summarized  as under. a)  Benefits to Consumers Reduced risk of food borne diseases Increased confidence in food supply Increased awar

Use pcr to sequence the particular gene, You wish to use PCR to sequence a ...

You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd