Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Will sedantary lifestyle increased risk for cad, Q. Will Sedantary Lifestyl...

Q. Will Sedantary Lifestyle increased risk for CAD? A sedantary life style is associated with increased risk for CAD. Sedentary persons have almost double the risk for CAD deat

Describe about the detectors used in hplc, Question 1 Explain pharmacokine...

Question 1 Explain pharmacokinetic parameters observed in plasma concentration time curve Question 2 What is Gas Chromatography? Mention the quantitative applications of gas

General guidelines of maintaining records of patients, General Guidelines ...

General Guidelines While working in the ward, you are always required to record. You should follow the general guidelines while recording which are as follows: All entri

Microorganisms, advantages and disadvantages of protozoa

advantages and disadvantages of protozoa

How different are the respiration in fishes, How different are the respirat...

How different are the respiration in fishes and the respiration in adult amphibians? In fishes gas exchange is completed by direct contact of water with the branchiae (gills).

Explain root amputation, Explain the Root amputation, Hemisectioning and Bi...

Explain the Root amputation, Hemisectioning and Bicuspidization Root amputation: removal of one or more roots of a multirooted tooth "upper teeth". Hemisectioning: surgic

Echinoderms, What is the classification scheme for echinoderms?

What is the classification scheme for echinoderms?

What are some fungal diseases transmitted by animal feces, What are some fu...

What are some fungal diseases transmitted by animal feces? Bat and pigeon feces can carry Histoplasma capsulatum, the fungus agent of histoplasmosis. The infection is transmitt

Explain the executive functions - psychological tests, Explain the Executiv...

Explain the Executive Functions - Psychological tests Executive functions are those involved in the planning, organisation, regulation and monitoring of goal directed behaviour

Patterns of cleavage, P A TTERNS OF CLEAVAGE - Patterns of cleavage d...

P A TTERNS OF CLEAVAGE - Patterns of cleavage depends upon amount of yolk & distribution of yolk. These are following types - 1 .       RADIAL CLEAVAGE PATTERN -

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd