Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

What is smooth muscle, What is Smooth Muscle? Smooth muscle provides th...

What is Smooth Muscle? Smooth muscle provides the contractile force for movement in internal organs under control of the involuntary or autonomic nervous system. Smooth muscle

Ethylene - apical dominance, Ethylene - Apical Dominance Ethylene also...

Ethylene - Apical Dominance Ethylene also plays a role in the inhibition of lateral bud growth. It is present in those tissues like shoot apex where auxins are also present. I

Explain phylum zooflagellata, Phylum Zooflagellata (Animal Flagellates)...

Phylum Zooflagellata (Animal Flagellates) 1) At some stage of life-cycle they have one or more flagella. 2) They feed heterotrophically by absorption or phagocytosis, and

Explain the conveyor (or band) dryers, Explain the Conveyor (or Band) Dryer...

Explain the Conveyor (or Band) Dryers? In the conveyor (or band) dryer, the product is distributed on a moving belt, typically of a perforated plate, that passes through a tunn

Which represents the least potential electromotive force, In a neuron with ...

In a neuron with a resting potential of -65 mV, the distribution of which ion across the neuronal membrane represents the LEAST potential electromotive force (EMF)? A. Potassium

Heredity and variations, HEREDITY 1.         Like begets like i.e; ever...

HEREDITY 1.         Like begets like i.e; every living organism reproduces its own kind. Frog reproduce frogs, rats reproduce rats only. 2.         Heredity includes the cha

Carbohydrates, Carbohydrates These form about 1%part of protoplasm, the...

Carbohydrates These form about 1%part of protoplasm, they are comparatively simpler compounds of carbon, hydrogen and oxygen in the ratio 1:2:1 The ratio hydrogen to oxygen is

Calcium - mineral elements, CALCIUM It is the most abundant mineral of...

CALCIUM It is the most abundant mineral of animal body. Calcium is available in all types of vegetables, grain, milk, cheese, eggs, fish and butter. By its deficiency r

Mastitis, M a s t iti s Mastitis is one the important diseases in ...

M a s t iti s Mastitis is one the important diseases in cows that has serious economic impact on livestock production. The inflammation of mammary gland is associated with

Human aging, what is the process called free radicals damage body molecules...

what is the process called free radicals damage body molecules

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd