Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

What are the archenteron and the blastopore, What are the archenteron and t...

What are the archenteron and the blastopore? What is the stage of the embryonic development in which these structures are formed? What are the destinations of the archenteron and o

Do plants present only sexual reproduction, Q. Do plants present only sexua...

Q. Do plants present only sexual reproduction? There are asexual forms of reproduction in plants few naturally detached pieces of root, leaves or limbs develop into another com

Examples on some plants species, examples on woody perennial Angiosperm spe...

examples on woody perennial Angiosperm species and herbaceous annual angiosperm species. thanks

#title.germ cell development, Ask questionhow are germ cells immortal #Mini...

Ask questionhow are germ cells immortal #Minimum 100 words accepted#

Malnutrition related diabetes mellitus, Q. Explain about Malnutrition Relat...

Q. Explain about Malnutrition Related Diabetes Mellitus? This type of diabetes has been categorized as a separate entity. 'if is 'Men seen commonly in developing countries, 1n

The hydrogen ion concentration of stomach, What is the hydrogen ion concent...

What is the hydrogen ion concentration of stomach acid pH=1.6?

Necrotic enteritis, N e c r otic enteritis It can be designated as ...

N e c r otic enteritis It can be designated as enterotoxaemia of chickens, turkeys and ducks caused by Clostridium perfringens. The toxin released by this spore-bearing a

Define reagents for measurement of ph, Define Reagents for Measurement of p...

Define Reagents for Measurement of pH? Buffer solutions of pH 4, pH 7 and pH 9.18 for calibration and an unknown solution (i.e. solution of unknown pH) You can prepare these

What is physiologic adaptation, Physiologic Adaptation Modeling is a su...

Physiologic Adaptation Modeling is a surface specific activity (apposition or resorption) that produces a net change in the size and/or shape of the bone. It is an uncoupled pr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd