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Phase Contrast MicroscopyPhase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).
It is a known fact that a light wave passing through a transparent object suffers a change in phase.The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.
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Glycogen is a huge polymer of glucose residues linked by α1-4 glycosidic bonds with branches every 10 residues or so by α1-6 glycosidic bonds. Glycogen gives an significant energy
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Mechanisms that Affect the Heart Rate Two different mechanisms affect the heart rate. Nerve impulse to the pacemaker region and hormonal influences. A branch of the vagues ner
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