Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

What are the genotypes, What are the genotypes and respective blood types o...

What are the genotypes and respective blood types of the ABO system? Since the alleles are IA, IB and i the possible genotypes are IAIA (blood type A), IAIB (blood type AB), IB

Process for estimation of sucrose by fehling soxhlet method, Explain Proced...

Explain Procedure for Estimation of Sucrose by Fehling Soxhlet Method? Follow the proceedure enumerated herewith and carry out the experiment: 1. Standard titration (Standar

Intermediate products of anaerobic respiration, (a) What are the intermedia...

(a) What are the intermediate products of anaerobic respiration in an active muscle? (b) Which of them is associated with oxygen debt? (c) In what way is th

Defination of behaviour change communication, Q. Defination of Behaviour Ch...

Q. Defination of Behaviour Change Communication? Behaviour Change Communication (BCC) is an interactive process with communities to develop specific messages and methods using

Common disease of camels, COMMON DISEASE OF CAMELS - 1 .       SURRA ...

COMMON DISEASE OF CAMELS - 1 .       SURRA - Surra = Rotten. Spread by biting & blood sucking flies, dropping neck, half closed eyes are common symptoms. 2 .

How a sample contaiinng this isotope decay, Please help with the following:...

Please help with the following: 32P is an unstable isotope of phosphorus that has a half-life of 14.29 days. Atoms of this isotope undergo beta decay in which a neutron decays i

Cnidarian, what process are nematocysts formed by the cnidoblast?

what process are nematocysts formed by the cnidoblast?

What are the reasons of food processing, What are the reasons of Food Proce...

What are the reasons of Food Processing? The reasons for food processing may vary, but the main objectives are to: Preserve the nutritive quality of food by preventing th

Define food effect in the survival & growth of microorganism, Define Food E...

Define Food Effects in the Survival & Growth of Microorganism? Nature of the food also affects the microbial survival and growth. Each food has its own characteristics which de

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd