Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Chilling and flower induction, Chilling and Flower Induction Some plan...

Chilling and Flower Induction Some plants flower only after passing a winter season. For example, winter wheat is sown in the autumn for harvest in the following summer. It ne

Explain carbonating - method of food preservation, Explain Carbonating - me...

Explain Carbonating - method of food preservation? Carbonated water is the water in which carbon dioxide gas has been dissolved under pressure. By eliminating oxygen, carbonate

Hormones secreted by thyroid gland, Hormone s . The thyroid gland secretes...

Hormone s . The thyroid gland secretes following hormones. Thyroxine (tetraiodothyronine or T 4 )' and tri-iodothyronine or T 3 are secreted by the thyroid follicular cells.

Symptoms of celiac disease, Q. Symptoms of celiac disease? The list of ...

Q. Symptoms of celiac disease? The list of symptoms as mentioned in various sources for celiac disease includes: • Digestive symptoms - diarrhoea, abdominal pain, abdominal

Strategies for genetic characterization and conservation, Strategies for ge...

Strategies for genetic characterization and conservation - During last 20 years concerted efforts have been made to create awareness among all the developmental organizations in th

What is serum sickness, When an individual is exposed to foreign serum anti...

When an individual is exposed to foreign serum antigen then a combination of symptoms are produced which is known as serum sickness.

Explain future challenges of connecting models and data, Explain Future cha...

Explain Future challenges of connecting models and data? Since biological systems are complex, models of biological systems are also complex, and matching models and data is a

What is the essential condition for protein to be identical, What is the es...

What is the essential condition for a protein to be identical to another protein? For a protein to be identical to another protein it is essential for the sequence of amino aci

Effect of ph or nutrient availability, Effect of pH or Nutrient Availabilit...

Effect of pH or Nutrient Availability One of the greatest influence of pH on plant growth is through its effect on the nutrient availability. When base saturation is less than

Amniocentesis, Amniocentesis is a way of prenatal testing in which the amn...

Amniocentesis is a way of prenatal testing in which the amniotic fluid is withdrawn from the uterus by the help of a needle. The fluid and the fetal cells it contains are analyzed

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd