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Phase Contrast MicroscopyPhase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).
It is a known fact that a light wave passing through a transparent object suffers a change in phase.The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.
What is meant by substrates of enzymatic reactions? Substrates are reagent molecules upon which enzymes act. The enzyme has spatial binding sites for the attachment of its s
General Characteristics of Protozoans The protozoans, are eukaryotes that form a group of about 80,000 single-celled organisms under the kingdom Protista. The unicellular leve
Haemocyanin - Respiratory Pigments This pigment is found in several arthropods and molluscs. Haemocyanins do not consist of haee groups. The metal they consist of is copper, w
9. A simple and inexpensive screening method has been developed to identify individuals with HIV infection at routine medical check-ups. To study the sensitivity and specificity, t
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Define Shell Fish as a rich source of protein? Information on shellfish is fragmentary and incomplete. In shell fish, the shell comprises of a large portion of live weight of t
The Cori cycle a) Pyruvate formed from glucose is converted to lactate by lactate dehydrogenase in the muscle cell. b) Lactate is released into the blood and taken up b
Explain the process of diagnosis of cholera Diagnosis: Cholera can be confirmed only by the isolation of the causative organism from the diarrheic stools of infected individual
Define Source of Electrons - Growth of Microorganism? Depending upon the source of electrons used by microorganisms, these are known as - a) Lithotrophs (rock eaters) - use
mode of nutrition : out side the body
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