Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Define effect of dietary fibre expect physiological effects, Define effect ...

Define effect of dietary fibre expect physiological effects? In addition to all the physiological effects mentioned above, dietary fibre may exert other effects. Acetate and pr

General process of post-hatching growth in insects, General process of post...

General process of post-hatching growth in insects While a young insect hatches out from an egg it is covered by a firm, inflexible, sclerotized cuticle, which because of its

protraction from hiv , Protraction from HIV  Under the following condi...

Protraction from HIV  Under the following conditions HIV is not transmitted 1) The secretions and body fluids like Saliva, Tears, Sweat, Faeces, and Urine have negligible HIV c

Nutrient cycles, Nutrient Cycles All ecosystems have certain common ba...

Nutrient Cycles All ecosystems have certain common basic features of structure and function. They all have living and non-living components through which there is a flow of en

Open or blunderbuss apex or divergent roots, Open or Blunderbuss Apex or di...

Open or Blunderbuss Apex or divergent roots The mechanical cleaning & shaping of teeth with blunderbuss canal apex are very difficult if not impossibly orthogratly due to 1

Vitamin - d, VITAMIN - D Also known as Calciferol / Anti ricket vit...

VITAMIN - D Also known as Calciferol / Anti ricket vitamin / sun shine vitamin. It is necessary for bones & teeth. It is synthesized in presence of UV rays of sunl

What resources are competed for by animals, What resources are competed for...

What resources are competed for by (a) animals, (b) plants?  (a) Animals compete for food, mates and shelter.  (b) Plants compete for light, water and minerals.

Explain about the supply curve or supply schedule, Explain about the supply...

Explain about the supply curve or supply schedule. The supply curve or schedule shows the total quantity of a good or a service supplied within aggregate, by whole sellers or f

Biological responses controlled by phytochrome, Biological Responses Contro...

Biological Responses Controlled by Phytochrome Phytochrome responses are those which are controlled reversibly by red and far-red light. These can be broadly categorised as:

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd