Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

What is the vacuum puff drying, What is the Vacuum Puff Drying? The vac...

What is the Vacuum Puff Drying? The vacuum puff process has been developed for drying liquids under vacuum. The development of processes for drying liquids under vacuum came fr

What is fern allies - equisetum, What is Fern Allies - Equisetum ? The ...

What is Fern Allies - Equisetum ? The earliest land plants are thought to have evolved from green algal ancestors, since they share many features. Both green algae and plants h

Types of stem cells-pulp tissue revascularization, Embryonic stem cells: c...

Embryonic stem cells: can differentiate into all cell types of the specialized embryonic tissues when given sufficient and necessary stimulation for a specific cell type, e.g. Plu

What is waist-to-hip ratio, What is Waist-to-Hip Ratio? Waist-to-Hip Ra...

What is Waist-to-Hip Ratio? Waist-to-Hip Ratio (WHR) is defined as the measurement of waist circumference divided by hip circumference. For example, if a person's waist measure

Explain prosthetic device infections, Explain Prosthetic device infections ...

Explain Prosthetic device infections Some infectious disease specialists recommend antimicrobial prophylaxis before process in patients with vascular grafts or orthopedic pros

Atrial arrhythmias-peri operative problems, Atrial Arrhythmias :  Atl...

Atrial Arrhythmias :  Atlial fibrillation is the most common rhythm abnormality after CABG. Paroxysmal atrial tachycardia and atrial flutter are less common. Increased sympat

Three phases into which the hiv infection is often divided, Q. What are the...

Q. What are the three phases into which the HIV infection is often divided? The HIV infection is frequently divided into three phases the acute phase, from the infection to 1 u

Which blood vessels carry venous blood to the heart, Q. What are the blood ...

Q. What are the blood vessels that carry venous blood to the heart? What is the blood vessel that collects arterial blood from the heart? The blood vessels that debouch in the

What do you mean by periodical, Q. What do you mean by Periodical? A pe...

Q. What do you mean by Periodical? A periodical is a publication appearing at regular intervals. Each issue is called a number and collectively these numbers comprise a volume.

Which type of gamete do the male respectively produce, Concerning the sex c...

Concerning the sex chromosomes of the XY system which type of gamete do the male and the female individuals respectively produce? The individual of the male sex is XY so he for

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd