Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Describe the number of implants, Number of Implants The use of more imp...

Number of Implants The use of more implants decreases the number of pontics and the associated mechanics and strains on the prosthesis, and dissipates stresses more effectively

Endoplasm and ectoplasm, Normal 0 false false false EN-...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain caspofungin, Explain Caspofungin It is available only for intra...

Explain Caspofungin It is available only for intravenous administration and is FDA-approved for treatment of esophageal candidiasis, candidemia, and intra-abdominal abscesses,

Define isotope dilution method, Define Isotope Dilution Method? The mea...

Define Isotope Dilution Method? The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance i

Phosphorus cycle - nutrient cycles, Phosphorus Cycle - Nutrient Cycles ...

Phosphorus Cycle - Nutrient Cycles Phosphorus is a very important nutrient because of its role in the form of phosphate, in reactions that store and release energy. The availa

Definition of diabetes mellitus, Q. DEFINITION OF DIABETES MELLITUS? Th...

Q. DEFINITION OF DIABETES MELLITUS? The word "diabetes" is derived from the Greek word meaning "a siphon". The patients of diabetes had polyuria (passing excessive urine) and "

Why humans good epithelial surfaces for respiration, Why is the skin on wor...

Why is the skin on worms, the gills in fish, and the lungs in humans good epithelial surfaces for respiration. Be specific.

Lipids, Lipids All animal tissues contain lipids or fats as essential...

Lipids All animal tissues contain lipids or fats as essential components of cell membrane. It is also stored in certain tissues. Lipids are body's chief source of energy and

What is the final comparative balance of these processes?, How the water ab...

How the water absorption volume and the water transpiration volume comparatively vary in plants in a day? What is the final comparative balance of these processes? Throughout t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd