Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

What factor cause the measurement to change, During gym class Sally noticed...

During gym class Sally noticed that her friend Melissa always ran faster than her. Sally knew that they exercised equally, so she wondered what could cause Melissa to run so fast.

Show cardiovascular risk factors, Q. Show Cardiovascular Risk Factors? ...

Q. Show Cardiovascular Risk Factors? It must be coming in your mind several times that why do some people suffer from heart disease while others do not? Well the most obvious r

Ecretory system, excretory product of marine teleost

excretory product of marine teleost

Define the growth charts of infant, Define the Growth Charts of Infant? ...

Define the Growth Charts of Infant? Besides identifying growth faltering, growth charts also provide the following information: 1) The growth is considered normal or satisfa

What is the meaning of paraplegia, What is the meaning of Paraplegia Pa...

What is the meaning of Paraplegia Paraplegia (from the Greek para, "alongside of," and plegia,  "stroke") is a condition in which both lower limbs are paralyzed (quadriplegia i

Iodine - mineral elements, IODINE The element is available is drinking ...

IODINE The element is available is drinking water, vegetables and fish. Table salts are also compulsorily iodized to provide iodine in the diet. All sea foods are rich in

Economically important fermentation products-rice beer, Normal 0 ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Respiratory organs, Respiratory Organs These are the organs regarded ...

Respiratory Organs These are the organs regarded with the gaseous exchange, i.e., intake .of oxygen and output of carbon dioxide. They have generally greater rate of gas exch

How can the reactions of the nervous system be classified, Q. Concerning vo...

Q. Concerning volition of the individual how can the reactions of the nervous system be classified? The efferences (reactions) of the nervous system can be classified into volu

Define x-ray chest and coronary angiography, Q. Define X-Ray Chest and Coro...

Q. Define X-Ray Chest and Coronary Angiography? X-Ray Chest It shows cardiomegaly with left ventricle (LV) and left atrium (LA) enlargement. There may be signs of pulmona

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd