Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

What is the difference between rna and dna, Q. Concerning the nitrogen- con...

Q. Concerning the nitrogen- containing bases that participate in nucleotides, what is the difference between RNA and DNA? In DNA nucleotides can be formed of thymine (T), adeni

Illustarte the utilization of soy protein concentrate, Utilization of Soy P...

Utilization of Soy Protein Concentrate Soy protein concentrates are used in food products for  their nutritional characteristics or for their functional properties or for both.

How fever, How Fever,Illness,Infections,Injury affects the BMR? Any ill...

How Fever,Illness,Infections,Injury affects the BMR? Any illness or fever caused by an illness influences the metabolic rate. Fevers increase the metabolic rate by about 7% for

How are animals divided according to their digestive process, Q. How are an...

Q. How are animals divided according to their kind of digestive process? At a distance from sponges that do not have a digestive cavity where extracellular digestion takes plac

Explain lactate dehydrogenase, Lactate dehydrogenase (LDH) is one of the im...

Lactate dehydrogenase (LDH) is one of the important enzymes in glycolysis which catalyzes the interconversion of pyruvate and lactate as follows:   Functional lactate d

Physiology of the heart, The heart is the muscular pump that pushes blood t...

The heart is the muscular pump that pushes blood through the circulatory system. Each heartbeat can be felt as an arterial pulse. The heart valves when closed prevent the blood fro

Explain the methodology for seliwanoff test, Explain the Methodology for se...

Explain the Methodology for seliwanoff test? Take 5 ml of seliwanoff's reagent in a test tube. Add 5-6 drops of the glucose solution and heat the mixture to boiling for about 3

Explain the virtual dissection of the rat, Explain the Virtual Dissection o...

Explain the Virtual Dissection of the Rat in multimedia tutorials? This animation will teach you about basic mammalian anatomy. The major organ systems in a rat are very simila

Difference between ribosome and plant virus, Difference between Ribosome an...

Difference between Ribosome and plant virus -   RIBOSOME (RNA + Protein)   PLANT VIRUS (RNA + Protein)   1. Does not mul

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd