Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Explain the principles of isolated soybean proteins, Principles of Isolated...

Principles of Isolated soybean proteins The basic principles of ISP production are simple. Soybean protein isolates are obtained by selective solubilisation of  the protein (e.

Cell, What is a Cell? The body of every living being is made up a livin...

What is a Cell? The body of every living being is made up a living matter or bio matter. In the organisms these matters occur only in the form of minute membrane bound unit lum

Competence of larval tissue or multiple responses, Competence of Larval Tis...

Competence of Larval Tissue or Multiple Responses The responsiveness of different larval tissues to thyroxine is markedly different. The tissues of the tail becomes necrotic

Explain restricted fragment polymorphism, Q. Explain Restricted fragment po...

Q. Explain Restricted fragment polymorphism? This method makes use of a group of enzymes termed restriction enzymes which recognise particular sequence of DNA and act as "molec

Determine nutritional requirements for high altitude, Determine Nutritional...

Determine Nutritional Requirements for High Altitude? High terrestrial altitudes and mountains have aroused great fascination and cham for mankind. Every year millions of peopl

Why is it dangerous to talk while eating, Q. Why is it dangerous to talk wh...

Q. Why is it dangerous to talk while eating? If a person talks when eating, food may be inhaled into the lungs. Generally after food is swallowed, it passes into pharynx and th

Define the role of addition of acetyl groups to histones, Creb binding prot...

Creb binding protein (CBP) is a histone acetyl transferase (HAT) protein. What role does the  addition of acetyl groups to histones play during development? A. The addition of

Infective endocarditis, Despite improvements in health care, the incidence ...

Despite improvements in health care, the incidence of infective endocarditis has not decreased over the past decades. Infective endocarditis is lethal if not aggressively treated w

Chromophore - development of plant, Chromophore - Development of plant ...

Chromophore - Development of plant The chromophore is a tetrapyrrole molecule like chlorophyll, but unlike chlorophyll it is an open tetrapyrrole and contains no metal ion. It

Telophase of karyokinesis, - The chromosomes at each pole uncoil and elonga...

- The chromosomes at each pole uncoil and elongate to form the chromatin. - A nucleolus reappears at each pole. - Spindle fibers and asters disappear and centrioles split. - A nu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd