Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Explain briefly Creutzfeldt-jakob disease (cjd), Normal 0 false...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Explain highly active antiretroviral therapy, Highly active antiretroviral ...

Highly active antiretroviral therapy (HAART) - The treatment of HIV infection requires combination therapy known as highly active antiretroviral therapy (HAART) along with inte

Single pair of alleles, Give three examples of human disorders which are ca...

Give three examples of human disorders which are caused by the action of a single pair of alleles. In every case say whether the harmful allele is dominant or recessive to the non-

Effect of water pollution, (i) Effect of Human Health: (a) Mercury co...

(i) Effect of Human Health: (a) Mercury compounds in waste water in converted into methyl mercury by Bacterial action which causes numbness of limbs, lips and tongue, deaf

Neutralize acid rain because water is buffered, Select all that are true or...

Select all that are true or correct regarding a buffer: alternately donating and accepting ions a set of chemicals that work together to resist pH change often a weak acid or base

Explain about precautionary principle, Q. Explain about precautionary princ...

Q. Explain about precautionary principle? The ethical values of biodiversity highlights the intrinsic value of biodiversity for its own sake and it is independent of the varied

Define introduction of lipids, Define Introduction of Lipids? You have ...

Define Introduction of Lipids? You have studied that lipids together with carbohydrates and proteins are important as food for many animals. Lipids include neutral fats, phosph

Protozoa, way of remember phylum protozoa

way of remember phylum protozoa

Psilopsida, why sporophyte of psilopsida is rootless

why sporophyte of psilopsida is rootless

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd