Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

What are the main human diseases caused by prions, Q. What are the main hum...

Q. What are the main human diseases caused by prions? The major known human diseases of such type are the Creutzfeldt-Jacob disease (CJD), the kuru and the Gerstmann-Sträussle-

Respiration, comparitive chart on different types of respiration obserbed i...

comparitive chart on different types of respiration obserbed in different type of respiration organ of respiration , types of respiration,energy obtained , by product formed

Application of derivative, Give some real world sample problem involving us...

Give some real world sample problem involving use of derivative

Explain the basic function and use of glycolysis, Q. What is the glycolysis...

Q. What is the glycolysis? And what are the products of this process? Glycolysis, the first stage of the aerobic cell respiration is a process in which glucose is degraded brok

Demand for health care in the presence of health insurance, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define isolated soybean proteins in dairy type products, Define isolated so...

Define isolated soybean proteins in Dairy type products? Soybean protein isolates are used in non-dairy coffee whiteners, liquid whipped toppings, emulsified sour cream or chee

MACRO TAXONOMY, WHAT IS CHARACTER SELECTION CRITERI

WHAT IS CHARACTER SELECTION CRITERIA

Social determinants of health - transport, Social Determinants of Health - ...

Social Determinants of Health - Transport Rising worldwide incomes have led to a massive use of automobiles and in particular cars. Cars are seen as a threat to health because

State the definition of bio-medical waste, State the definition of Bio-medi...

State the definition of Bio-medical waste 'Bio-medical waste' means any solid and/or liquid waste including its container and any intermediate product, which is generated durin

Explain fungal infections, Fungal Infections Intravenous infusion of am...

Fungal Infections Intravenous infusion of amphotericin B deoxycholate  frequently causes fever and chills, and sometimes headache, nausea, vomiting, hypotension and tachypnea,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd