Phase contrast microscopy, Biology

Assignment Help:

Phase Contrast Microscopy

Phase contrast microscope has the resolving power similar to ordinary light microscope. However, it enables us to observe the cellular organelles in living cell. The working of this microscope is based on the principle of phase contrast.

To understand phase contrast microscopy you should know that whenever two light waves interfere with each other, the resultant light wave may have either greater or lesser amplitude depending on the phases of the light waves (Figure shown below).

988_phase microscope1.jpg


It is a known fact that a light wave passing through a transparent object suffers a change in phase.

The living cells are heterogeneous in their cellular components having different thickness. Due to this difference, each component has different refractive index. Hence, when a light wave passes through the cell, a change in phase is produced due to differences of thickness and refractive indices of cellular components. The phase contrast microscope exaggerates the small difference between the phases and enables one to distinguish the adjacent structures.


Related Discussions:- Phase contrast microscopy

Explain precautions for performing completed coliform test, Explain Precaut...

Explain Precautions for Performing the Completed Coliform Test 1. Aseptic conditions should be adopted while performing the experiment. 2. Destaining is the critical step in

What are the cells that produce the myelin sheath, What are the cells that ...

What are the cells that produce the myelin sheath? Of which substance is the myelin sheath formed? In the central nervous system (CNS) the myelin sheath is made by apposition o

Difference between primary ecological succession, Q. What is the difference...

Q. What is the difference between primary ecological succession and secondary ecological succession? The Primary ecological succession is the changing sequence of communities f

Describe the recovery process of an extracellular product, Question 1: ...

Question 1: Describe the recovery process of an extracellular product through downstream processing. Different criteria's for the choice of recovery process Brief on

Excretory organs, why the excretory organ of prawn is called ''green gland'...

why the excretory organ of prawn is called ''green gland''?

Describe the impact and burden of heart diseases, Describe the IMPACT AND B...

Describe the IMPACT AND BURDEN OF HEART DISEASES ? No discussion on the epidemiology of heart diseases will be complete without a look at the tremendous burden put by different

Haccp control measure, HACCP Control Measure HACCP Control Measure : ...

HACCP Control Measure HACCP Control Measure :  Any action or activity that can be used to prevent, eliminate or reduce a significant hazard.

Analysis of different milk samples, could you please give me a data in the ...

could you please give me a data in the form of tabular column based on milk analysis which can be done in home itself?

Define sex as a determinants of nutrient requirements, Define Sex as a dete...

Define Sex as a determinants of nutrient requirements? There are some differences in requirements between the two sexes. However, except for iron, these are apparent rather tha

What are the organic chemical groups, Q. What are the organic chemical grou...

Q. What are the organic chemical groups that describe carbohydrates? How are carbohydrates classified according to the presence of those groups? Carbohydrates are also known as

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd